LOCUS       sequence-39464-e        7138 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Retroviral expression of mouse Glis1.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7138)
  AUTHORS   Maekawa M, Yamaguchi K, Nakamura T, Shibukawa R, Kodanaka I, 
            Ichisaka T, Kawamura Y, Mochizuki H, Goshima N, Yamanaka S
  TITLE     Direct reprogramming of somatic cells is promoted by maternal 
            transcription factor Glis1.
  JOURNAL   Nature. 2011  . 474(7350):225-9.
  PUBMED    21654807
REFERENCE   2  (bases 1 to 7138)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nature. 
            2011  . 474(7350):225-9."
COMMENT     Sequence Label: pMXs-mGLIS1
FEATURES             Location/Qualifiers
     source          1..7138
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             22..615
                     /note="long terminal repeat from Moloney murine leukemia 
                     virus"
     misc_feature    678..1035
                     /label=MMLV Psi
                     /note="packaging signal of Moloney murine leukemia virus 
                     (MMLV)"
     CDS             1100..1516
                     /label=gag (truncated)
                     /note="truncated Moloney murine leukemia virus (MMLV) gag 
                     gene lacking the start codon"
     primer_bind     1446..1468
                     /label=pLXSN 5'
                     /note="Murine stem cell virus, forward primer. Also called 
                     MSCV"
     primer_bind     1484..1500
                     /label=pBABE 5'
                     /note="Psi packaging signal, forward primer for pBABE 
                     vectors"
     misc_feature    1526..1900
                     /label=pol region
                     /note="Moloney murine leukemia virus (MMLV) pol region 
                     containing the splice acceptor site"
     primer_bind     1838..1854
                     /label=pBMN 5'
                     /note="MMLV sequence, for inserts in pBMN retroviral 
                     vector"
     protein_bind    1951..1975
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(4364..4388)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     LTR             4583..5176
                     /note="long terminal repeat from Moloney murine leukemia 
                     virus"
     primer_bind     complement(5242..5259)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(5413..6001)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(5493..5512)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(6165..6956,6957..7025))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     6788..6807
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(7026..7130)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 cccgaaaagt gccacctgca taatgaaaga ccccacctgt aggtttggca agctagctta
       61 agtaacgcca ttttgcaagg catggaaaaa tacataactg agaatagaaa agttcagatc
      121 aaggtcagga acagatggaa cagctgaata tgggccaaag cggatatctg tggtaagcag
      181 ttcctgcccc ggctcagggc caagaacaga tggaacagct gaatatgggc caaacaggat
      241 atctgtggta agcagttcct gccccggctc agggccaaga acagatggtc cccagatgcg
      301 gtccagccct cagcagtttc tagagaacca tcagatgttt ccagggtgcc ccaaggacct
      361 gaaatgaccc tgtgccttat ttgaactaac caatcagttc gcttctcgct tctgttcgcg
      421 cgcttctgct ccccgagctc aataaaagag cccacaaccc ctcactcggg gcgccagtcc
      481 tccgattgac tgagtcgccc gggtacccgt gtatccaata aaccctcttg cagttgcatc
      541 cgacttgtgg tctcgctgtt ccttgggagg gtctcctctg agtgattgac tacccgtcag
      601 cgggggtctt tcatttgggg gctcgtccgg gatcgggaga cccctgccca gggaccaccg
      661 acccaccacc gggaggtaag ctggccagca acttatctgt gtctgtccga ttgtctagtg
      721 tctatgactg attttatgcg cctgcgtcgg tactagttag ctaactagct ctgtatctgg
      781 cggacccgtg gtggaactga cgagttcgga acacccggcc gcaaccctgg gagacgtccc
      841 agggatctcg ggggccgttt ttgtggcccg acctgagtcc aaaaatcccg atcgttttgg
      901 actctttggt gcacccccct tagaggaggg atatgtggtt ctggtaggag acgagaacct
      961 aaaacagttc ccgcctccgt ctgaattttt gctttcggtt tgggaccgaa gccgcgccgc
     1021 gcgtcttgtc tgctgcagca tcgttctgtg ttgtctctgt ctgactgtgt ttctgtattt
     1081 gtctgaaaat atgggcccgg gccagactgt taccactccc ttaagtttga ccttaggtca
     1141 ctggaaagat gtcgagcgga tcgctcacaa ccagtcggta gatgtcaaga agagacgttg
     1201 ggttaccttc tgctctgcag aatggccaac ctttaacgtc ggatggccgc gagacggcac
     1261 ctttaaccga gacctcatca cccaggttaa gatcaaggtc ttttcacctg gcccgcatgg
     1321 acacccagac caggtcccct acatcgtgac ctgggaagcc ttggcttttg acccccctcc
     1381 ctgggtcaag ccctttgtac accctaagcc tccgcctcct cttcctccat ccgccccgtc
     1441 tctccccctt gaacctcctc gttcgacccc gcctcgatcc tccctttatc cagccctcac
     1501 tccttctcta ggcgccccca tatggccata tgagatctta tatggggcac ccccgcccct
     1561 tgtaaacttc cctgaccctg acatgacaag agttactaac agcccctctc tccaagctca
     1621 cttacaggct ctctacttag tccagcacga agtctggaga cctctggcgg cagcctacca
     1681 agaacaactg gaccgaccgg tggtacctca cccttaccga gtcggcgaca cagtgtgggt
     1741 ccgccgacac cagactaaga acctagaacc tcgctggaaa ggaccttaca cagtcctgct
     1801 gaccaccccc accgccctca aagtagacgg catcgcagct tggatacacg ccgcccacgt
     1861 gaaggctgcc gaccccgggg gtggaccatc ctctagactg ccggatctag ctagttaatt
     1921 aaggatccca gtgtggtggt acgggaaatc acaagtttgt acaaaaaagc aggcttcgaa
     1981 ggagatagaa ccatgcattg cgaggtggcc gaggcacttt cggacaagag gccaaaggag
     2041 gcccctggtg ctcctggcca gggccgcggg cctgtcagcc tgggagcgca catggccttc
     2101 aggattgctg tgagtggtgg cggctgcggg gacgggaacc cgctagacct gctgcctcgg
     2161 ctaccggtgc caccaccacg tgcccacgat ctccttcggc cccggagccc tcgagactat
     2221 ggtgtgtcca agaccggcag cgggaaggtg aacgggagct acgggcacag ctcagagaag
     2281 agcctgctgg acctggacct ggccgagggt cccagcccct cctgccacca gggtctgttt
     2341 cttcctgcag ggaccccacc accccggggt cacccccctg tctgtgagaa gctgctgcac
     2401 ttcccccacc caaacaggtc acccagacct caggctacgt ttgtgaacgg cagcctccca
     2461 gccgctcagc acatcaagca agaagcccta ccggactacc aggccatggt cagcgcccac
     2521 acacccctgc ccacccactg ccgagcccca tcgtccatgg gtctgccctc agacctggac
     2581 tttccagacc gaggcctcac caaccctgca ccttcctgct accttctggg caatgaaccc
     2641 atctcagacc tgggtcccca acccgaggcc cacctccccg agggcagcct gaaacgctgc
     2701 tgcctcctgg gcctgccccc cacctcttca gcctcctcct caccctgtgc ctcctcagat
     2761 atcaatcctg tcatccactc ctcccagaca gctctagtta gctgtgtaaa tggactccga
     2821 agcccacctc tgccgggaga cctggggggc cctcccaagc ggtcacggcc cgggcctgca
     2881 tccagtgacg gccaggaggg cagcttgcag cttgaagcat gccggaagtc aggcttcctg
     2941 aagcaggagc ccatggacga gttttcagag ctttttgctc cacaccacca gggtttgcca
     3001 cccccttacc ccttgcctca gttgccaact ggccccggcc tcggaggcct agggctgggc
     3061 ctggcaggta ggatggttgc cggtcggcag gcatgccgct gggtggactg ctgcgcagcc
     3121 tacgagcagc aggaggagct ggtgcggcac atcgagaaga gccacatcga ccagcgcaag
     3181 ggcgaagact tcacctgctt ctgggccggg tgtgtgcggc gctacaagcc cttcaatgcc
     3241 cgctacaagc tgctcatcca catgagggta cactcaggcg agaagcccaa caagtgcatg
     3301 ttcgaaggct gcagtaaagc cttttcccgt ctggagaacc tgaagatcca tctgcggagc
     3361 cacacaggcg agaaaccata cctgtgccag cacccaggct gccagaaggc cttcagcaac
     3421 tccagcgacc gtgccaagca ccaacgcacc cacctcgaca cgaagccata tgcttgtcag
     3481 atccctggct gctccaagcg ctacacggac cccagctccc tccgcaagca cgtgaaggcc
     3541 cactcagcca aagagcagca ggtgcgtaag aagctgcaca caggtgccga cccagaggct
     3601 gatgttctgt ccgagtgtct gtccctgcag cagctccaag catccacact gttgccggcc
     3661 agcagaggga agggcagcca aaccctgagc caggagctcc tcccaggtgt gtatcctggc
     3721 tccgtcaccc cacaaaacgg gcttgcttca ggcatcctgt ccccctccca cgatgtccct
     3781 tccaggcacc acccactgga ggtccccact ggttcccacc accacctgtc ccctctgccc
     3841 acagctgaga gcaccaggga tggcctgggg cccagtctcc tttcacccat ggtcagccca
     3901 ctgaaggggc ttggtccccc accgctacca ccagcctccc agagtcagtc tccaggggga
     3961 cagtcattct ctacagtccc cagcaagcct acctacccat ccttccaaag cccaccacct
     4021 ctgcccagcc cccaaggcta ccaaggcagt ttccattcca tccagaactg cttcccctac
     4081 gctgactgct accgggccac tgagccagca gcctccaggg atggactggt gggtgatgcc
     4141 cacggtttca accccttgcg acccagcaca tactccagcc tcagcacacc tttatccgca
     4201 ccaggctacg agaccctggc agaaacgccg tgtcccccag cgctgcagcc acagccagct
     4261 gaagacctgg tacctagtgg tcctgaggac tgtggcttct tccccaatgg ggcctttgac
     4321 cactgtctga gtcacatccc gtccatctac actgacacct aagacccagc tttcttgtac
     4381 aaagtggtga tattccagct gagcgccggt cgctaccatt accagttggt ctggtgtcaa
     4441 aaataataat aaccgggcag gccatgtctg cccgtatttc gcgtaaggaa atccattatg
     4501 tactatttaa actcgagcgg ccgccagcac agtggtcgac gataaaataa aagattttat
     4561 ttagtctcca gaaaaagggg ggaatgaaag accccacctg taggtttggc aagctagctt
     4621 aagtaacgcc attttgcaag gcatggaaaa atacataact gagaatagag aagttcagat
     4681 caaggtcagg aacagatgga acagctgaat atgggccaaa caggatatct gtggtaagca
     4741 gttcctgccc cggctcaggg ccaagaacag atggaacagc tgaatatggg ccaaacagga
     4801 tatctgtggt aagcagttcc tgccccggct cagggccaag aacagatggt ccccagatgc
     4861 ggtccagccc tcagcagttt ctagagaacc atcagatgtt tccagggtgc cccaaggacc
     4921 tgaaatgacc ctgtgcctta tttgaactaa ccaatcagtt cgcttctcgc ttctgttcgc
     4981 gcgcttctgc tccccgagct caataaaaga gcccacaacc cctcactcgg ggcgccagtc
     5041 ctccgattga ctgagtcgcc cgggtacccg tgtatccaat aaaccctctt gcagttgcat
     5101 ccgacttgtg gtctcgctgt tccttgggag ggtctcctct gagtgattga ctacccgtca
     5161 gcgggggtct ttcattctgc attaatgaat cggccaacgc gcggggagag gcggtttgcg
     5221 tattgggcgc tcttccgctt cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg
     5281 gcgagcggta tcagctcact caaaggcggt aatacggtta tccacagaat caggggataa
     5341 cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc
     5401 gttgctggcg tttttccata ggctccgccc ccctgacgag catcacaaaa atcgacgctc
     5461 aagtcagagg tggcgaaacc cgacaggact ataaagatac caggcgtttc cccctggaag
     5521 ctccctcgtg cgctctcctg ttccgaccct gccgcttacc ggatacctgt ccgcctttct
     5581 cccttcggga agcgtggcgc tttctcatag ctcacgctgt aggtatctca gttcggtgta
     5641 ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc gttcagcccg accgctgcgc
     5701 cttatccggt aactatcgtc ttgagtccaa cccggtaaga cacgacttat cgccactggc
     5761 agcagccact ggtaacagga ttagcagagc gaggtatgta ggcggtgcta cagagttctt
     5821 gaagtggtgg cctaactacg gctacactag aaggacagta tttggtatct gcgctctgct
     5881 gaagccagtt accttcggaa aaagagttgg tagctcttga tccggcaaac aaaccaccgc
     5941 tggtagcggt ggtttttttg tttgcaagca gcagattacg cgcagaaaaa aaggatctca
     6001 agaagatcct ttgatctttt ctacggggtc tgacgctcag tggaacgaaa actcacgtta
     6061 agggattttg gtcatgagat tatcaaaaag gatcttcacc tagatccttt tgcggccggc
     6121 cgcaaatcaa tctaaagtat atatgagtaa acttggtctg acagttacca atgcttaatc
     6181 agtgaggcac ctatctcagc gatctgtcta tttcgttcat ccatagttgc ctgactcccc
     6241 gtcgtgtaga taactacgat acgggagggc ttaccatctg gccccagtgc tgcaatgata
     6301 ccgcgagacc cacgctcacc ggctccagat ttatcagcaa taaaccagcc agccggaagg
     6361 gccgagcgca gaagtggtcc tgcaacttta tccgcctcca tccagtctat taattgttgc
     6421 cgggaagcta gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt tgccattgct
     6481 acaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc cggttcccaa
     6541 cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa aagcggttag ctccttcggt
     6601 cctccgatcg ttgtcagaag taagttggcc gcagtgttat cactcatggt tatggcagca
     6661 ctgcataatt ctcttactgt catgccatcc gtaagatgct tttctgtgac tggtgagtac
     6721 tcaaccaagt cattctgaga atagtgtatg cggcgaccga gttgctcttg cccggcgtca
     6781 acacgggata ataccgcgcc acatagcaga actttaaaag tgctcatcat tggaaaacgt
     6841 tcttcggggc gaaaactctc aaggatctta ccgctgttga gatccagttc gatgtaaccc
     6901 actcgtgcac ccaactgatc ttcagcatct tttactttca ccagcgtttc tgggtgagca
     6961 aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa atgttgaata
     7021 ctcatactct tcctttttca atattattga agcatttatc agggttattg tctcatgagc
     7081 ggatacatat ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg cacatttc
//
