LOCUS       sequence-394677-         687 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  FIP200 NTD-MBP mammalian expression.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 687)
  AUTHORS   Chen M, Ren X, Nguyen TN, Khuu G, Cook ASI, Lazarou M, Hurley JH
  TITLE     Structure and activation of the human autophagy-initiating 
            ULK1C:PI3KC3-C1 supercomplex
  JOURNAL   bioRxiv 2023.06.01.543278
REFERENCE   2  (bases 1 to 687)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRxiv 
            2023.06.01.543278"
COMMENT     Sequence Label: pCAG-FIP200  (1-640)-GST
FEATURES             Location/Qualifiers
     source          1..687
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             1..21
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
     CDS             25..678
                     /codon_start=1
                     /product="glutathione S-transferase from Schistosoma 
                     japonicum"
                     /label=GST
                     /translation="MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKK
                     FELGLEFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVLDIRY
                     GVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTHPDFMLYDALDVVL
                     YMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIAWPLQGWQATFGGGDHPPK"
     primer_bind     636..658
                     /label=pGEX 5'
                     /note="pGEX vectors, Glutathione-S-transferase, forward 
                     primer"
ORIGIN
        1 gaaaacctgt acttccaatc cggtatgtcc cctatactag gttattggaa aattaagggc
       61 cttgtgcaac ccactcgact tcttttggaa tatcttgaag aaaaatatga agagcatttg
      121 tatgagcgcg atgaaggtga taaatggcga aacaaaaagt ttgaattggg tttggagttt
      181 cccaatcttc cttattatat tgatggtgat gttaaattaa cacagtctat ggccatcata
      241 cgttatatag ctgacaagca caacatgttg ggtggttgtc caaaagagcg tgcagagatt
      301 tcaatgcttg aaggagcggt tttggatatt agatacggtg tttcgagaat tgcatatagt
      361 aaagactttg aaactctcaa agttgatttt cttagcaagc tacctgaaat gctgaaaatg
      421 ttcgaagatc gtttatgtca taaaacatat ttaaatggtg atcatgtaac ccatcctgac
      481 ttcatgttgt atgacgctct tgatgttgtt ttatacatgg acccaatgtg cctggatgcg
      541 ttcccaaaat tagtttgttt taaaaaacgt attgaagcta tcccacaaat tgataagtac
      601 ttgaaatcca gcaagtatat agcatggcct ttgcagggct ggcaagccac gtttggtggt
      661 ggcgaccatc ctccaaaaat tgactag
//
