LOCUS       sequence-394678-        1920 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  FIP200 NTD-TwinStrep-Flag mammalian expression.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1920)
  AUTHORS   Chen M, Ren X, Nguyen TN, Khuu G, Cook ASI, Lazarou M, Hurley JH
  TITLE     Structure and activation of the human autophagy-initiating 
            ULK1C:PI3KC3-C1 supercomplex
  JOURNAL   bioRxiv 2023.06.01.543278
REFERENCE   2  (bases 1 to 1920)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRxiv 
            2023.06.01.543278"
COMMENT     Sequence Label: pCAG-FIP200 (1-640)-TSF
FEATURES             Location/Qualifiers
     source          1..1920
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 atgaagctct atgtgtttct cgtgaatacc ggcactaccc tgacctttga taccgaactg
       61 accgtccaga ccgtggctga cctgaagcat gctatccagt ctaaatacaa gatcgcaatt
      121 cagcaccagg tgctggtggt caacggcgga gagtgcatgg ccgctgatcg cagggtctgt
      181 acctattcag ccggcaccga cactaaccca atcttcctct ttaataagga gatgattctg
      241 tgcgatagac cccctgctat ccccaagacc acttttagca ctgagaacga catggaaatc
      301 aaagtggagg aatccctgat gatgccagcc gtgttccaca ccgtcgcttc tcgcactcag
      361 ctggcactcg agatgtacga agtggccaag aaactgtgct ctttctgtga gggtctcgtc
      421 cacgatgaac atctgcagca ccagggctgg gcagccatta tggctaacct ggaggactgt
      481 tcaaatagtt atcagaaact gctcttcaag tttgagtcta tctactcaaa ctatctgcag
      541 agcatcgaag acattaaact gaagctcaca cacctgggaa ccgcagtgag cgtcatggcc
      601 aagatccctc tgctcgagtg cctgactagg cattcctaca gagaatgtct gggtaggctc
      661 gatagtctgc cagaacacga ggacagcgag aaagccgaaa tgaagcgctc tacagagctg
      721 gtgctctcac ctgacatgcc aaggacaacc aacgaaagtc tgctcaccag cttcccaaag
      781 tccgtggagc atgtctcccc cgacactgcc gatgctgaat ctggaaagga gattagagaa
      841 agttgccaga gcaccgtgca ccagcaggac gagactacaa ttgacactaa ggacggcgat
      901 ctgcccttct ttaacgtcag tctgctcgat tggatcaacg tccaggacag acctaatgat
      961 gtggagtccc tggtccggaa gtgctttgat agcatgtccc gtctggaccc acgtatcatt
     1021 cgtcctttca tcgcagagtg tcgtcagacc attgccaagc tggacaacca gaatatgaaa
     1081 gctattaagg gtctggaaga tcggctctac gccctggacc agatgatcgc tagctgtgga
     1141 cgcctggtga acgagcagaa ggaactcgct cagggtttcc tggcaaacca gaaaagagcc
     1201 gagaatctca aggatgcatc agtgctgcct gacctgtgcc tcagtcatgc caatcagctg
     1261 atgattatgc tccagaacca ccgcaagctg ctcgacatca aacagaagtg taccactgct
     1321 aaacaggagc tggcaaacaa tctccatgtg aggctgaagt ggtgctgttt cgtcatgctg
     1381 cacgcagacc aggatggcga gaagctccag gccctgctca ggctggtgat tgagctgctc
     1441 gaaagagtga agatcgtcga ggctctgtcc accgtcccac agatgtactg cctggcagtg
     1501 gtcgaggtgg tcagacgtaa aatgtttatc aagcactaca gagaatgggc aggtgccctc
     1561 gtgaaagatg gcaagcgtct gtatgaagcc gagaaatcta agcgtgagtc attcggcaaa
     1621 ctgtttcgga agagcttcct ccgtaaccgg ctgttccgcg gactcgacag ttggccaccc
     1681 agcttttgca cccagaaacc taggaagttc gactgtgagc tgccagatat ctccctcaag
     1741 gacctgcagt ttctccagtc cttctgccca tctgaggtgc agccctttct gagggtccct
     1801 ctgctctgtg acttcgaacc tctgcaccag catgtgctgg ccctccacaa cctcgtcaag
     1861 gctgcacaga gtctggatga gatgagccag acaatcaccg acctgctcag cgaacagaag
//
