LOCUS       sequence-394683-        1149 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Mammalian expression of ATG13 C-terminal and ULK1 MIT domain fusion 
            complex.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1149)
  AUTHORS   Chen M, Ren X, Nguyen TN, Khuu G, Cook ASI, Lazarou M, Hurley JH
  TITLE     Structure and activation of the human autophagy-initiating 
            ULK1C:PI3KC3-C1 supercomplex
  JOURNAL   bioRxiv 2023.06.01.543278
REFERENCE   2  (bases 1 to 1149)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRxiv 
            2023.06.01.543278"
COMMENT     Sequence Label: pCAG-TSF-ATG13 (363-517)-ULK1 (836-1050)
FEATURES             Location/Qualifiers
     source          1..1149
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             1..21
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
ORIGIN
        1 gaaaacctgt acttccaatc ccacgatgtc ctggaaacca tcttcgtgag aaaagtcggc
       61 gcttttgtga acaagcccat taatcaggtc acactcacct ctctggacat ccctttcgct
      121 atgtttgcac caaagaacct cgagctggaa gacactgatc ccatggtgaa tcctccagac
      181 tcacccgaga cagaaagtcc tctccagggc agtctgcaca gcgatggaag ctccggtggc
      241 tcttcaggca acacacatga cgatttcgtc atgattgact tcaaacccgc tttttccaag
      301 gacgatatcc tccctatgga tctgggaacc ttctacaggg agtttcagaa tccccctcag
      361 ctcagtagcc tgagcattga catcggtgcc cagtccatgg ctgaggacct cgattctctg
      421 cctgaaaaac tggctgtgca cgagaagaac gtcagagaat tcgacgcatt tgtggagacc
      481 ctgcagggta gcgacgaagc gatggagcag gaacacactg agatcctgag gggtctcaga
      541 ttcaccctgc tctttgtgca gcatgtcctg gaaattgctg cactcaaggg tagtgctagc
      601 gaggcagctg gtggaccaga ataccagctg caggagagcg tggtcgccga ccagatctct
      661 ctgctctcaa gggaatgggg cttcgctgag cagctggtcc tctatctgaa ggtggccgaa
      721 ctgctcagta gcggcctgca gtccgccatc gatcagatta gagctggaaa gctctgcctg
      781 tcctctactg tgaaacaggt ggtccggcgc ctgaacgagc tctacaaggc cagtgtggtc
      841 agctgtcagg gactctctct gcgtctccag cggttctttc tggacaaaca gcgcctgctc
      901 gataggatcc actcaattac agctgaacgc ctgatcttca gccatgcagt gcagatggtc
      961 cagtccgcag ccctggacga aatgtttcag cacagggagg gatgcgtccc cagataccat
     1021 aaggctctgc tcctgctcga gggtctgcag cacatgctca gcgaccaggc cgatatcgaa
     1081 aatgtgacta agtgcaaact gtgtattgag aggagactct ccgctctgct cacaggcatc
     1141 tgtgcatga
//
