LOCUS       sequence-394689-         489 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Mammalian expression of TwinStrep-Flag tagged ATG13 (363-517).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 489)
  AUTHORS   Chen M, Ren X, Nguyen TN, Khuu G, Cook ASI, Lazarou M, Hurley JH
  TITLE     Structure and activation of the human autophagy-initiating 
            ULK1C:PI3KC3-C1 supercomplex
  JOURNAL   bioRxiv 2023.06.01.543278
REFERENCE   2  (bases 1 to 489)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRxiv 
            2023.06.01.543278"
COMMENT     Sequence Label: pCAG-TSF-ATG13 (363-517)
FEATURES             Location/Qualifiers
     source          1..489
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             1..21
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
ORIGIN
        1 gaaaacctgt acttccaatc ccacgatgtc ctggaaacca tcttcgtgag aaaagtcggc
       61 gcttttgtga acaagcccat taatcaggtc acactcacct ctctggacat ccctttcgct
      121 atgtttgcac caaagaacct cgagctggaa gacactgatc ccatggtgaa tcctccagac
      181 tcacccgaga cagaaagtcc tctccagggc agtctgcaca gcgatggaag ctccggtggc
      241 tcttcaggca acacacatga cgatttcgtc atgattgact tcaaacccgc tttttccaag
      301 gacgatatcc tccctatgga tctgggaacc ttctacaggg agtttcagaa tccccctcag
      361 ctcagtagcc tgagcattga catcggtgcc cagtccatgg ctgaggacct cgattctctg
      421 cctgaaaaac tggctgtgca cgagaagaac gtcagagaat tcgacgcatt tgtggagacc
      481 ctgcagtaa
//
