LOCUS       sequence-39469-e         609 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expression of the eGFP reporter gene was not detected in the brain 
            and eye..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 609)
  AUTHORS   Portales-Casamar E, Swanson DJ, Liu L, de Leeuw CN, Banks KG, Ho Sui
            SJ, Fulton DL, Ali J, Amirabbasi M, Arenillas DJ, Babyak N, Black 
            SF, Bonaguro RJ, Brauer E, Candido TR, Castellarin M, Chen J, Chen 
            Y, Cheng JC, Chopra V, Docking TR, Dreolini L, D'Souza CA, Flynn EK,
            Glenn R, Hatakka K, Hearty TG, Imanian B, Jiang S, Khorasan-zadeh S,
            Komljenovic I, Laprise S, Liao NY, Lim JS, Lithwick S, Liu F, Liu J,
            Lu M, McConechy M, McLeod AJ, Milisavljevic M, Mis J, O'Connor K, 
            Palma B, Palmquist DL, Schmouth JF, Swanson MI, Tam B, Ticoll A, 
            Turner JL, Varhol R, Vermeulen J, Watkins RF, Wilson G, Wong BK, 
            Wong SH, Wong TY, Yang GS, Ypsilanti AR, Jones SJ, Holt RA, 
            Goldowitz D, Wasserman WW, Simpson EM
  TITLE     A regulatory toolbox of MiniPromoters to drive selective expression 
            in the brain.
  JOURNAL   Proc Natl Acad Sci U S A. 2010 Sep 21. 107(38):16589-94.
  PUBMED    20807748
REFERENCE   2  (bases 1 to 609)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A. 2010 Sep 21. 107(38):16589-94."
COMMENT     Sequence Label: pEMS1360
FEATURES             Location/Qualifiers
     source          1..609
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      15..24
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 ctcgcccttg ctcaccatgg tggcggaagt tcctatacct tttgaagaat aggaacttcg
       61 gcgcgcctga gggcgggcat ggctggggtg agctctctgg tcccacctgg ccatttatgc
      121 cctggtcctg aatggggtgg gtccaggcag cacgggtaat cacatttgtc tagcggggaa
      181 tccaattttc cctcccacaa gcttgcgccc caggccaagc actcagcctg ctgccctcct
      241 ctaattggca ctaatgacac atggacgtca tcagtgggag ttgaagtaga caggcggatg
      301 ctgtcccttc tgagatggct ctgaagagcc cgtttgtaca atactagcag gtgggcccat
      361 ttcaaaccaa aaataagaca atctccaaat agtccctgtg tttagacact ctctccacgg
      421 cctgctcttc ccacttttgt ctcctctcaa tttttccaca gcctccagct ggtaccactg
      481 ctgctccaga aggcgcgaga gcatcatagg gagtggggga catgggcgtg gggtgcattg
      541 ctggggacag acggagctgg agctgcacag gggataggca ggctgcaggg tgcactgctg
      601 ggggtgaca
//
