LOCUS       sequence-394709-         594 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Mammalian expression of GFP tagged ATG13 (1-197).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 594)
  AUTHORS   Chen M, Ren X, Nguyen TN, Khuu G, Cook ASI, Lazarou M, Hurley JH
  TITLE     Structure and activation of the human autophagy-initiating 
            ULK1C:PI3KC3-C1 supercomplex
  JOURNAL   bioRxiv 2023.06.01.543278
REFERENCE   2  (bases 1 to 594)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRxiv 
            2023.06.01.543278"
COMMENT     Sequence Label: pCAG-msfGFP-ATG13 (1-197)
FEATURES             Location/Qualifiers
     source          1..594
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             527..538
                     /codon_start=1
                     /product="WELQut protease recognition and cleavage site"
                     /label=WELQut site
                     /translation="WELQ"
ORIGIN
        1 atggagaccg acctcaacag ccaggaccgt aaagacctcg acaagttcat taagtttttc
       61 gccctcaaga ccgtgcaggt gatcgtgcag gcccggctgg gagagaaaat ctgcacacgc
      121 agctcctctt cacccaccgg ttctgactgg ttcaacctgg ctattaagga tatccctgaa
      181 gtgactcacg aagcaaagaa agcactcgct ggacagctgc cagcagtggg tcgttcaatg
      241 tgtgtcgaga tctccctgaa gacatctgaa ggagactcaa tggagctcga aatttggtgc
      301 ctggagatga acgaaaagtg tgataaagag atcaaagtga gctacaccgt ctataatcgt
      361 ctgtccctgc tcctgaaatc tctcctggcc atcactcggg tgacacctgc ttaccgcctc
      421 agtaggaagc agggtcatga atatgtgatt ctgtacagga tctatttcgg cgaggtccag
      481 ctctccggac tgggtgaagg ctttcagact gtgcgtgtcg gtaccgtggg aactccagtc
      541 ggcacaatta ccctctcttg cgcataccgt atcaatctgg ccttcatgtc atga
//
