LOCUS       sequence-394711-        1167 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Mammalian expression of ATG13 (363-517) with MBP tag.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1167)
  AUTHORS   Chen M, Ren X, Nguyen TN, Khuu G, Cook ASI, Lazarou M, Hurley JH
  TITLE     Structure and activation of the human autophagy-initiating 
            ULK1C:PI3KC3-C1 supercomplex
  JOURNAL   bioRxiv 2023.06.01.543278
REFERENCE   2  (bases 1 to 1167)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRxiv 
            2023.06.01.543278"
COMMENT     Sequence Label: pCAG-ATG13 (363-517)-MBP
FEATURES             Location/Qualifiers
     source          1..1167
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             1..21
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
     CDS             25..1125
                     /codon_start=1
                     /gene="malE (mutated)"
                     /product="maltose binding protein from E. coli"
                     /label=MBP
                     /note="This version of the gene does not encode a signal 
                     sequence, so MBP will remain in the cytosol."
                     /translation="MKIEEGKLVIWINGDKGYNGLAEVGKKFEKDTGIKVTVEHPDKLE
                     EKFPQVAATGDGPDIIFWAHDRFGGYAQSGLLAEITPDKAFQDKLYPFTWDAVRYNGKL
                     IAYPIAVEALSLIYNKDLLPNPPKTWEEIPALDKELKAKGKSALMFNLQEPYFTWPLIA
                     ADGGYAFKYENGKYDIKDVGVDNAGAKAGLTFLVDLIKNKHMNADTDYSIAEAAFNKGE
                     TAMTINGPWAWSNIDTSKVNYGVTVLPTFKGQPSKPFVGVLSAGINAASPNKELAKEFL
                     ENYLLTDEGLEAVNKDKPLGAVALKSYEEELAKDPRIAATMENAQKGEIMPNIPQMSAF
                     WYAVRTAVINAASGRQTVDEALKDAQT"
     primer_bind     1099..1122
                     /label=MBP-F
                     /note="Maltose binding protein, forward primer"
     CDS             1135..1164
                     /codon_start=1
                     /product="10xHis affinity tag"
                     /label=10xHis
                     /translation="HHHHHHHHHH"
ORIGIN
        1 gaaaacctgt acttccaatc cggtatgaaa atcgaagaag gtaaactggt aatctggatt
       61 aacggcgata aaggctataa cggtctcgct gaagtcggta agaaattcga gaaagatacc
      121 ggaattaaag tcaccgttga gcatccggat aaactggaag agaaattccc acaggttgcg
      181 gcaactggcg atggccctga cattatcttc tgggcacacg accgctttgg tggctacgct
      241 caatctggcc tgttggctga aatcaccccg gacaaagcgt tccaggacaa gctgtatccg
      301 tttacctggg atgccgtacg ttacaacggc aagctgattg cttacccgat cgctgttgaa
      361 gcgttatcgc tgatttataa caaagatctg ctgccgaacc cgccaaaaac ctgggaagag
      421 atcccggcgc tggataaaga actgaaagcg aaaggtaaga gcgcgctgat gttcaacctg
      481 caagaaccgt acttcacctg gccgctgatt gctgctgacg ggggttatgc gttcaagtat
      541 gaaaacggca agtacgacat taaagacgtg ggcgtggata acgctggcgc gaaagcgggt
      601 ctgaccttcc tggttgacct gattaaaaac aaacacatga atgcagacac cgattactcc
      661 atcgcagaag ctgcctttaa taaaggcgaa acagcgatga ccatcaacgg cccgtgggca
      721 tggtccaaca tcgacaccag caaagtgaat tatggtgtaa cggtactgcc gaccttcaag
      781 ggtcaaccat ccaaaccgtt cgttggcgtg ctgagcgcag gtattaacgc cgccagtccg
      841 aacaaagagc tggcaaaaga gttcctcgaa aactatctgc tgactgatga aggtctggaa
      901 gcggttaata aagacaaacc gctgggtgcc gtagcgctga agtcttacga ggaagagttg
      961 gcgaaagatc cacgtattgc cgccactatg gaaaacgccc agaaaggtga aatcatgccg
     1021 aacatcccgc agatgtccgc tttctggtat gccgtgcgta ctgcggtgat caacgccgcc
     1081 agcggtcgtc agactgtcga tgaagccctg aaagacgcgc agactggcag tagccaccat
     1141 caccatcacc atcatcacca tcactag
//
