LOCUS       sequence-394718-         705 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Mammalian expression of GST tagged ATG14.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 705)
  AUTHORS   Chen M, Ren X, Nguyen TN, Khuu G, Cook ASI, Lazarou M, Hurley JH
  TITLE     Structure and activation of the human autophagy-initiating 
            ULK1C:PI3KC3-C1 supercomplex
  JOURNAL   bioRxiv 2023.06.01.543278
REFERENCE   2  (bases 1 to 705)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRxiv 
            2023.06.01.543278"
COMMENT     Sequence Label: pCAG-GST-ATG14
FEATURES             Location/Qualifiers
     source          1..705
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             1..657
                     /codon_start=1
                     /product="glutathione S-transferase from Schistosoma 
                     japonicum"
                     /label=GST
                     /translation="MASPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNK
                     KFELGLEFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVLDIR
                     YGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTHPDFMLYDALDVV
                     LYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIAWPLQGWQATFGGGDHPPK"
     primer_bind     615..637
                     /label=pGEX 5'
                     /note="pGEX vectors, Glutathione-S-transferase, forward 
                     primer"
     CDS             685..705
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
ORIGIN
        1 atggcatccc ctatactagg ttattggaaa attaagggcc ttgtgcaacc cactcgactt
       61 cttttggaat atcttgaaga aaaatatgaa gagcatttgt atgagcgcga tgaaggtgat
      121 aaatggcgaa acaaaaagtt tgaattgggt ttggagtttc ccaatcttcc ttattatatt
      181 gatggtgatg ttaaattaac acagtctatg gccatcatac gttatatagc tgacaagcac
      241 aacatgttgg gtggttgtcc aaaagagcgt gcagagattt caatgcttga aggagcggtt
      301 ttggatatta gatacggtgt ttcgagaatt gcatatagta aagactttga aactctcaaa
      361 gttgattttc ttagcaagct acctgaaatg ctgaaaatgt tcgaagatcg tttatgtcat
      421 aaaacatatt taaatggtga tcatgtaacc catcctgact tcatgttgta tgacgctctt
      481 gatgttgttt tatacatgga cccaatgtgc ctggatgcgt tcccaaaatt agtttgtttt
      541 aaaaaacgta ttgaagctat cccacaaatt gataagtact tgaaatccag caagtatata
      601 gcatggcctt tgcagggctg gcaagccacg tttggtggtg gcgaccatcc tccaaaatcg
      661 gatgattacg atatcccaac gaccgaaaac ctgtattttc agggt
//
