LOCUS       sequence-394739-       10871 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  pBAD322-MycaIDCas-PaqCI, arapBAD promoter expressing type I-D 
            Cascade and one spacer array of McCAST (Tn7575), the single spacer 
            is replaced with PaqCI entry site..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10871)
  AUTHORS   Hsieh SC, Peters JE
  TITLE     Discovery and characterization of novel type I-D CRISPR-guided 
            transposons identified among diverse Tn7-like elements in 
            cyanobacteria.
  JOURNAL   Nucleic Acids Res. 2023 Jan 25;51(2):765-782. doi: 
            10.1093/nar/gkac1216.
  PUBMED    36537206
REFERENCE   2  (bases 1 to 10871)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10"; journalName: 
            "Nucleic Acids Res"; date: "2023-01-25- 25"; volume: "51"; issue: 
            "2"; pages: "765-782"
COMMENT     Sequence Label: pOPO685
FEATURES             Location/Qualifiers
     source          1..10871
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(90..968)
                     /codon_start=1
                     /gene="araC"
                     /product="L-arabinose regulatory protein"
                     /label=araC
                     /translation="MAEAQNDPLLPGYSFNAHLVAGLTPIEANGYLDFFIDRPLGMKGY
                     ILNLTIRGQGVVKNQGREFVCRPGDILLFPPGEIHHYGRHPEAREWYHQWVYFRPRAYW
                     HEWLNWPSIFANTGFFRPDEAHQPHFSDLFGQIINAGQGEGRYSELLAINLLEQLLLRR
                     MEAINESLHPPMDNRVREACQYISDHLADSNFDIASVAQHVCLSPSRLSHLFRQQLGIS
                     VLSWREDQRISQAKLLLSTTRMPIATVGRNVGFDDQLYFSRVFKKCTGASPSEFRAGCE
                     EKVNDVAVKLS"
     promoter        995..1279
                     /gene="araBAD"
                     /label=araBAD promoter
                     /note="promoter of the L-arabinose operon of E. coli; the 
                     araC regulatory gene is transcribed in the opposite 
                     direction (Guzman et al., 1995)"
     primer_bind     1202..1221
                     /label=pBAD Forward
                     /note="For vectors with E. coli araBAD promoter, forward 
                     primer"
     primer_bind     complement(7311..7328)
                     /label=pBAD Reverse
                     /note="For vectors with E. coli araBAD promoter, reverse 
                     primer"
     terminator      7481..7567
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      7659..7686
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     promoter        7705..7796
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(7797..7865,7866..8657)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(8015..8034)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      8699..9154
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(8786..8805)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     8996..9017
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     rep_origin      9265..9853
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     9754..9773
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     10007..10024
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    10039..10179
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(10105..10124)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     10265..10287
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     CDS             complement(10281..10472)
                     /codon_start=1
                     /gene="rop"
                     /product="Rop protein, which maintains plasmids at low copy
                     number"
                     /label=rop
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
ORIGIN
        1 gcataatgtg cctgtcaaat ggacgaagca gggattctgc aaaccctatg ctactccgtc
       61 aagccgtcaa ttgtctgatt cgttaccaat tatgacaact tgacggctac atcattcact
      121 ttttcttcac aaccggcacg gaactcgctc gggctggccc cggtgcattt tttaaatacc
      181 cgcgagaaat agagttgatc gtcaaaacca acattgcgac cgacggtggc gataggcatc
      241 cgggtggtgc tcaaaagcag cttcgcctgg ctgatacgtt ggtcctcgcg ccagcttaag
      301 acgctaatcc ctaactgctg gcggaaaaga tgtgacagac gcgacggcga caagcaaaca
      361 tgctgtgcga cgctggcgat atcaaaattg ctgtctgcca ggtgatcgct gatgtactga
      421 caagcctcgc gtacccgatt atccatcggt ggatggagcg actcgttaat cgcttccatg
      481 cgccgcagta acaattgctc aagcagattt atcgccagca gctccgaata gcgcccttcc
      541 ccttgcccgg cgttaatgat ttgcccaaac aggtcgctga aatgcggctg gtgcgcttca
      601 tccgggcgaa agaaccccgt attggcaaat attgacggcc agttaagcca ttcatgccag
      661 taggcgcgcg gacgaaagta aacccactgg tgataccatt cgcgagcctc cggatgacga
      721 ccgtagtgat gaatctctcc tggcgggaac agcaaaatat cacccggtcg gcaaacaaat
      781 tctcgtccct gatttttcac caccccctga ccgcgaatgg tgagattgag aatataacct
      841 ttcattccca gcggtcggtc gataaaaaaa tcgagataac cgttggcctc aatcggcgtt
      901 aaacccgcca ccagatgggc attaaacgag tatcccggca gcaggggatc attttgcgct
      961 tcagccatac ttttcatact cccgccattc agagaagaaa ccaattgtcc atattgcatc
     1021 agacattgcc gtcactgcgt cttttactgg ctcttctcgc taaccaaacc ggtaaccccg
     1081 cttattaaaa gcattctgta acaaagcggg accaaagcca tgacaaaaac gcgtaacaaa
     1141 agtgtctata atcacggcag aaaagtccac attgattatt tgcacggcgt cacactttgc
     1201 tatgccatag catttttatc cataagatta gcggatccta cctgacgctt tttatcgcaa
     1261 ctctctactg tttctccata cccgtttttt tgggctagca ggaggaattc accatgaagc
     1321 ccttactcca gactcttcta cttcaaactc taccgtctga cattgatcca gtattgctgg
     1381 cttatgttga gactgtatta cctgctgtag aacaggagtt cggtctgatt ccagtttcta
     1441 acgatgttag tcaaactgag caaagcttgc tggttcacat tctcaatagt ttgctgactg
     1501 cctggaactt gttcccgttc ctatcctcaa aattggcgct atcagacaac gagaaacgat
     1561 tgctttgctt gagtcttatc tttcaaaatg acaacagtaa gtgccagagc cgagagaata
     1621 attcgcctcc tacgcttggt gttaatgaaa ccctaaaaca gtaccgagca tttggagaca
     1681 aactaaatct ggcggcgttc tggactgagt ggcgtgagta tttgacagat atagtttatt
     1741 taacccaaag cacccaaccg catgctttgt caatcaagaa gactgctctc aaaattcctg
     1801 ctaatcgcct agagttgcct ctacgtccat tgcttctgtt tggggcgatc gctgcccagc
     1861 ttactgatcc cgctgatatt gtcatcaaac cggaaggcaa tgtcttaaaa gaatgcttga
     1921 aacgtctggg tgttcagcat caactcactt atcatcgagt acgcgcctgc acaggactct
     1981 taactgaggg catccacaat gccattatgc gttttgtaca acctctcgac tggcaatcca
     2041 ttctgttctt tgcccaaggg atcgtttatt tggttccctc tgacagcaaa ctgcttgatt
     2101 gctcaaaact acaaaccttt atctgggagc agattagtga gcagttagca atcagtatgc
     2161 ttcgaggaaa agttggtttc aagcgagatg gcaaagggat gaaagctgcg cctcaaacgc
     2221 tggagctatt ctcaccagct caattcattc ggaatctacc cactgtcatt aatgcctgtg
     2281 tcaagaatga gacggaacct gcaacaccta aacgactggc aaatcttgac cttgatccaa
     2341 cagatcgaga atttttagct tgtggggcgg atctaagaag cgatcgcatt gcagaattta
     2401 tccttttctt gcagaagaag ttttttggga ctaatcctga atttatcgat cgagttctcg
     2461 accaactggg cattcaatct gccatcactc cagaacagac tcaaatcttc tcaagcgggg
     2521 taaatagggg atggtatcac gcagccgctt attacgtcgc cagtaaccct cacttagact
     2581 cagatgatgt aacgatcaag cttcaggaga tcgccaatca actagtggac tgggctgaag
     2641 agaaagaact ttttccacct caaatcagtc cgactcgtga ggttttcaat caatacttag
     2701 ctcagtactt ggaattccca aggtggaata attcagcgcc atcctttcaa catgaattgg
     2761 caacgtatgt agcggcaaaa acaaaagccg ctaaacaacc aatttgctcc ctcagttcca
     2821 gtgaatttga ctcagaagac cagctagatt ctgttgtgct attcaagcct cagcagtata
     2881 gcaacaagaa tcccctcgga gggtggcaac tgaagcgagg aatttctaaa ctctggtcat
     2941 tagagatgct tctgcggcag tctttctggt cagtttccgc aggcaagctg gaagagcgac
     3001 aacctgtctt tctgtacttt tttcccagtt tggttcattc ccctcaggtc gcaaaagcga
     3061 ttcgtatcct agtcaatcaa cttaaacgag tcaatttgtg gaaagtttgt cgattctggc
     3121 aggtacacgg gatgaatgct aataccctgc gcttctactc ttggctgaag tctgatccca
     3181 gaccagacga taatggtaaa cgtgatctcc ctttcctagc aatcacttac accaccacag
     3241 ctggtaaaac gacgacacag gcttggattg aacctgcttt tttggtattg gcgctctcac
     3301 ttgtgctggg cattaaagta gtcgctacgg caagtccagt accactttat aacaggagta
     3361 gcgagttccc cgaaacagtg aagatagacg gacctgcgag cttctggagc ctgttgggtt
     3421 tgccaaccag cattcggctg gaagagaaga tccagggacg ggtcaaacac ctgaatgatt
     3481 ggctcgatcg cctactaatt gcttacagta ttcaccttga ctgtcaagcg aatccccctg
     3541 actctcgttg gcaattgttt cccggtacag tgcaggctat aacaactgat gtctcagcga
     3601 tttttagcct tgctcattcg cattttcaaa gtctttcgga tgacagagtt caacgatact
     3661 ggcactttgc tcaagtatgg gcagcaggag attcaaacat ggaacagcaa ttggagctaa
     3721 ctcagcgact ggtccaggaa tatcgcgcgt tctaccaagt tcatgtagcg aaagcaaccc
     3781 acgcgatcgt gctcccgctg ctaaaggttt tagaggagat tctttcaact cctagtgatc
     3841 tgccaattga aactctgata ttacagacat cgggacggtt acatgacgct ttgaagcgac
     3901 aggaacccta caaacgcccc ctgattatgg atcagtcttt atcaattgag attcgtgtca
     3961 ataacgagat tcaggcaatt caacgatttg tcaccacttg catcaatgat ttatttttag
     4021 ctcagtacaa aggcgatcgc gccctactgc aagaacaccg taaccgaatt aaatcgggtg
     4081 cagagtttgc gtatcgttta ctagctatgc aggagccaaa accaaaaacg aaaaagcaag
     4141 aaattgaagg agcacagata tgagtgtgtt agaagaattg aaacctcaat tccattcggc
     4201 ttttccccgt ctggcatccg ggaagtatgt tcatgtcttc ctggtacgtc acagtcaatc
     4261 ctttcccgtg tttcaaacag atggtgtcct caatacggct cgaactcaag ctggcttgga
     4321 ttccaaagat gataaatcta ggcaatcgat caaccgattg gtcatgttca agcgcaagca
     4381 agtaacaccc gaacgactgg ttggacgaca actgctacga tcgctaggac taaccagtgc
     4441 tgtagaaggc gaagtcaact tttgcgaata caacagtcag aaatcgtgca agaaatgccc
     4501 agactgtatt atctacggat tcggggttgg agacaaaggt gcagaacgct cgaaagtcta
     4561 ctcagactca gcgttctcgc tcagtcctta tgaaacttcc caccgcagtt tcaccttcaa
     4621 tgcgccgtca gaaaatggca cgatgagtga aaagggagaa ctgcgaagtt cgatcaacga
     4681 tctagaccat gtcctgccgg aagtgatgtt tcctagtgtt gaaacgctgc gtgatcccac
     4741 atttgagggg tttatttatg tgctgggcaa cctgattcgc acgcgccgct atggaggtca
     4801 agaatcccga accgggacga tgatgaatca cattgttggt attgcctttt gcgatggtga
     4861 agttttcagc aacttgtatt tcacacaagc gctatatgat gccttgaatg caaaggggga
     4921 tatttgcgtg acgatcgagg actttcgaca tgaggcagca agggtggcaa aaaactgtct
     4981 gaaacaggag ccagttcgca gcatcacaat ttttggggct gaactcgatg ccttgctcga
     5041 agaagtgacg ggcatctatc aggatgaagt taaacagcgc cttattatga ccacgcttta
     5101 ccaacagacc cagatctaca gtgaaagcta cggagcaatt tttcctgaga agaagaaaga
     5161 ggaaaagaaa aaagcacagc aaggctcttc gatctgaggg atggtggaat aatggttttc
     5221 tatttttgcc atttaacctt gcatgacaat gttttctttg cctcacggga gatgggaact
     5281 ctctatgaga cagaaaaata cctgcacaat tgggcactta gctatgcgct atttgaaagc
     5341 gcctacattc ctagacctta tcgattggaa ggggcagtcg cccagcgtcc tacgtatcta
     5401 gagattgata gtgagcaaaa cctgttacat ctgaatcagc aaggaattta tgtttttccc
     5461 gccctgccgc ttcagtggac atatcaggtc aatacattca atgtgtctca agccttctac
     5521 catcaaaaat caagacggtt gggctcggga ggacatattc tcaattaccc aatcagctat
     5581 ggacgggcaa aggaactcgc agttggcagc aagtacagaa ccttcatcat tgctcctgcc
     5641 aaaacgagaa tcccccgctg gataagacta ggcaaatggt cagcgaaaat tcgagtcgag
     5701 atcatcccca ttcctaaaag tggaattcaa agggcagcag gcgagtatgt ttctgagcat
     5761 cccctcaatc ctgtagatct ctctaaaacg acccgattat tgctgtataa ccggattgtc
     5821 atgccacctt ctagtctggt gagccaggca aaattgttgg gtgactattg ggagatctca
     5881 aaaccagctc agtgggctaa attgtctgag tggtcaagct tagagaattt gcctgaaacg
     5941 atttgtttgc caagaggagt tgcttatgga gctaacactc tcgttaccac accttgaagc
     6001 gacagcattg cagacattgg tgattaaact cggggctgcg ggcaatggag atttttcctt
     6061 atcctgtagc cgagcacttc atgctcaagt tctgaaatgg tttcagttag gacaaccaca
     6121 aatttctcaa gcaattcatg atagtcagga gtctcccatc agcctctcag gactaatggg
     6181 caagcgcttt ggaagagttg tgcaggaggg agatgaattc tactttcggg ttgggttact
     6241 gaatggaaat ctgcttgaac cgttgttgaa agggctagag cagtgggaaa gtcaagtagt
     6301 gctcctttct aaatttcctt ttgttatcaa agatatcaat atgttgccag gtagccactt
     6361 tctggtcggc tcttcagact acactctgct tgcccaaatg ccttcagacg caaaagcgct
     6421 aacactaagg ttcctctctc cgactagctt taagctcgct caaaatcggt acattcaacc
     6481 agttccattg cccgattcag tttttggaag tttacagcgt cgctggaata cctttgctcc
     6541 caaagagtta cggtttccca aaactcaatg ggcgggattg atttgtgact ttgaaattaa
     6601 atcccagcgg ctaaatctgg acaggacagt tgagctgggt tccgttggat gggttagata
     6661 ccagtttccc gatttgcaac aagctagaat tgcaaacgtt ttggctcact ttgccttctt
     6721 ctcaggagta ggacgcaaaa cggcaatggg catgggattg actctactta aaggaacaac
     6781 tccaagttga gtgtgaccaa aactagggca tagacttgtg gcagactgca gcaagttcca
     6841 aatcttcaga aggtttgcta aaactattaa aatgcttaag actcaaggct ctactcattt
     6901 ctcatgatat aaataagttc aataattcct ggctttaagg gctgaatctt aactttccta
     6961 gaatcagctt tgccaaagac atggttaaga ttttgtcacg gttggctctg agtatgctaa
     7021 aggctcacga aacatccccc ggtttgcggg actgaaaggt aagcaggtgt aaggccacct
     7081 gcgtaaaact cgtgaagcat ctcccgattt gcgggattgt aaaattatac gacttcaccc
     7141 ataggttgaa ctttaagtcg tgatcagaac acaacacgcg agaagaacca gaatggcaag
     7201 tattactgcg atcgcttagt ggagtggatt ctaggtaccc ggggatcctc tagagtcgac
     7261 ctgcaggcat gcaagcttgg ctgttttggc ggatgagaga agattttcag cctgatacag
     7321 attaaatcag aacgcagaag cggtctgata aaacagaatt tgcctggcgg cagtagcgcg
     7381 gtggtcccac ctgaccccat gccgaactca gaagtgaaac gccgtagcgc cgatggtagt
     7441 gtggggtctc cccatgcgag agtagggaac tgccaggcat caaataaaac gaaaggctca
     7501 gtcgaaagac tgggcctttc gttttatctg ttgtttgtcg gtgaacgctc tcctgagtag
     7561 gacaaatccg ccgggagcgg atttgaacgt tgcgaagcaa cggcccggag ggtggcgggc
     7621 aggacgcccg ccataaactg ccaggcatca aattaagcag aaggccatcc tgacggatgg
     7681 cctttttgcg tttctacaaa ctcttttgtt tatttttcta aatacattca aatatgtatc
     7741 cgctcatgag acaataaccc tgataaatgc ttcaataata ttgaaaaagg aagagtatga
     7801 gtattcaaca tttccgtgtc gcccttattc ccttttttgc ggcattttgc cttcctgttt
     7861 ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga agatcagttg ggtgcacgag
     7921 tgggttacat cgaactggat ctcaacagcg gtaagatcct tgagagtttt cgccccgaag
     7981 aacgttttcc aatgatgagc acttttaaag ttctgctatg tggcgcggta ttatcccgtg
     8041 ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat gacttggttg
     8101 agtactcacc agtcacagaa aagcatctta cggatggcat gacagtaaga gaattatgca
     8161 gtgctgccat aaccatgagt gataacactg cggccaactt acttctgaca acgatcggag
     8221 gaccgaagga gctaaccgct tttttgcaca acatggggga tcatgtaact cgccttgatc
     8281 gttgggaacc ggagctgaat gaagccatac caaacgacga gcgtgacacc acgatgcctg
     8341 tagcaatggc aacaacgttg cgcaaactat taactggcga actacttact ctagcttccc
     8401 ggcaacaatt aatagactgg atggaggcgg ataaagttgc aggaccactt ctgcgctcgg
     8461 cccttccggc tggctggttt attgctgata aatctggagc cggtgagcgt gggtctcgcg
     8521 gtatcattgc agcactgggg ccagatggta agccctcccg tatcgtagtt atctacacga
     8581 cggggagtca ggcaactatg gatgaacgaa atagacagat cgctgagata ggtgcctcac
     8641 tgattaagca ttggtaactg tcagaccaag tttactcata tatactttag attgatttac
     8701 gcgccctgta gcggcgcatt aagcgcggcg ggtgtggtgg ttacgcgcag cgtgaccgct
     8761 acacttgcca gcgccctagc gcccgctcct ttcgctttct tcccttcctt tctcgccacg
     8821 ttcgccggct ttccccgtca agctctaaat cgggggctcc ctttagggtt ccgatttagt
     8881 gctttacggc acctcgaccc caaaaaactt gatttgggtg atggttcacg tagtgggcca
     8941 tcgccctgat agacggtttt tcgccctttg acgttggagt ccacgttctt taatagtgga
     9001 ctcttgttcc aaactggaac aacactcaac cctatctcgg gctattcttt tgatttataa
     9061 gggattttgc cgatttcggc ctattggtta aaaaatgagc tgatttaaca aaaatttaac
     9121 gcgaatttta acaaaatatt aacgtttaca atttaaaagg atctaggtga agatcctttt
     9181 tgataatctc atgaccaaaa tcccttaacg tgagttttcg ttccactgag cgtcagaccc
     9241 cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt
     9301 gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac
     9361 tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg tccttctagt
     9421 gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct
     9481 gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga
     9541 ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac
     9601 acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagctatg
     9661 agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa gcggcagggt
     9721 cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc
     9781 tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt caggggggcg
     9841 gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct tttgctggcc
     9901 ttttgctcac atgttctttc ctgcgttatc ccctgattct gtggataacc gtattaccgc
     9961 ctttgagtga gctgataccg ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag
    10021 cgaggaagcg gaagagcgcc tgatgcggta ttttctcctt acgcatctgt gcggtatttc
    10081 acaccgcata tggtgcactc tcagtacaat ctgctctgat gccgcatagt taagccagta
    10141 tacactccgc tatcgctacg tgactgggtc atggctgcgc cccgacaccc gccaacaccc
    10201 gctgacgcgc cctgacgggc ttgtctgctc ccggcatccg cttacagaca agctgtgacc
    10261 gtctccggga gctgcatgtg tcagaggttt tcaccgtcat caccgaaacg cgcgaggcag
    10321 ctgcggtaaa gctcatcagc gtggtcgtga agcgattcac agatgtctgc ctgttcatcc
    10381 gcgtccagct cgttgagttt ctccagaagc gttaatgtct ggcttctgat aaagcgggcc
    10441 atgttaaggg cggttttttc ctgtttggtc actgatgcct ccgtgtaagg gggatttctg
    10501 ttcatggggg taatgatacc gatgaaacga gagaggatgc tcacgatacg ggttactgat
    10561 gatgaacatg cccggttact ggaacgttgt gagggtaaac aactggcggt atggatgcgg
    10621 cgggaccaga gaaaaatcac tcagggtcaa tgccagctag cagcacgcca tagtgactgg
    10681 cgatgctgtc ggaatggacg atatcccgca agaggcccgg cagtaccggc ataaccaagc
    10741 ctatgcctac agcatccagg gtgacggtgc cgaggatgac gatgagcgca ttgttagatt
    10801 tcatacacgg tgcctgactg cgttagcaat ttaactgtga taaactaccg cattaaagct
    10861 agcttatcga t
//
