LOCUS       sequence-394742-        4889 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Plasmid expressing an antigen targeting the Kvbeta2 potassium 
            channel subunit.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4889)
  TITLE     Trimmer lab antigen plasmids
REFERENCE   2  (bases 1 to 4889)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: KvB2/RBG4
FEATURES             Location/Qualifiers
     source          1..4889
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    2..18
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     7..29
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     26..42
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     26..42
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     promoter        63..81
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     enhancer        176..555
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        556..759
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     710..730
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     756..780
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     polyA_signal    1988..2122
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(2025..2044)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     2079..2098
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(2140..2159)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(2141..2159)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(2166..2183)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(2166..2182)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2175..2197)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      2324..2779
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(2411..2430)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     2621..2642
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        2805..2909
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(2910..2978,2979..3770)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(3128..3147)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      3941..4529
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4430..4449
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     4683..4700
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    4817..4838
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(4853..4858,4859..4876,4877..4883)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
ORIGIN
        1 attgtgagcg gataacaatt tcacacagga aacagctatg accatgatta cgccaagctc
       61 gaaattaacc ctcactaaag ggaacaaaag ctggagcttg gcccattgca tacgttgtat
      121 ccatatcata atatgtacat ttatattggc tcatgtccaa cattaccgcc atgttgacat
      181 tgattattga ctagttatta atagtaatca attacggggt cattagttca tagcccatat
      241 atggagttcc gcgttacata acttacggta aatggcccgc ctggctgacc gcccaacgac
      301 ccccgcccat tgacgtcaat aatgacgtat gttcccatag taacgccaat agggactttc
      361 cattgacgtc aatgggtgga gtatttacgg taaactgccc acttggcagt acatcaagtg
      421 tatcatatgc caagtacgcc ccctattgac gtcaatgacg gtaaatggcc cgcctggcat
      481 tatgcccagt acatgacctt atgggacttt cctacttggc agtacatcta cgtattagtc
      541 atcgctatta ccatggtgat gcggttttgg cagtacatca atgggcgtgg atagcggttt
      601 gactcacggg gatttccaag tctccacccc attgacgtca atgggagttt gttttggcac
      661 caaaatcaac gggactttcc aaaatgtcgt aacaactccg ccccattgac gcaaatgggc
      721 ggtaggcgtg tacggtggga ggtctatata agcagagctc gtttagtgaa ccgtcagatc
      781 gcctggagac gccatccacg ctgttttgac ctccatagaa gacaccggga ccgatccagc
      841 ctgactctag ataagtgagg ccggccccat gtatccggaa tcaaccacgg ggtctccagc
      901 tcgactctcc ctgcggcaga caggctcccc cgggatgatc tacagtactc ggtatgggag
      961 tcccaaaaga cagctccagt tttacaggaa tctgggcaaa tctggccttc gggtctcctg
     1021 cctggggctt ggaacatggg tgaccttcgg aggccagatc acagatgaga tggcagagca
     1081 cctaatgacc ttggcctatg acaatggcat caacctgttc gatacggcgg aggtctacgc
     1141 agctggcaag gctgaagtgg tattagggaa catcattaag aagaaggggt ggagacggtc
     1201 cagccttgtc atcaccacca agatcttctg gggcggaaag gcagagaccg agagaggcct
     1261 ttcccggaag cacataatcg aaggactgaa agcttccctg gagaggctgc agctggagta
     1321 cgtggatgtg gtttttgcca accgcccaga ccccaacaca cccatggaag agactgtgcg
     1381 ggccatgacc catgtcatca accaagggat ggccatgtac tggggcacat cacgctggag
     1441 ctccatggag atcatggagg cctactcggt ggctcggcag ttcaacctga tcccgcccat
     1501 ctgcgagcaa gcggaatacc acatgttcca gagggagaag gtagaggtcc agctgccaga
     1561 gctgttccac aagataggag taggtgccat gacctggtcc cctctggcct gcggcattgt
     1621 ctcagggaag tatgacagtg gcatcccacc ctactccaga gcctctctga agggctacca
     1681 gtggttgaag gacaagatcc tgagtgagga gggtcgccgc cagcaagcca aactgaagga
     1741 actgcaggcc attgcagagc gcctaggctg caccctccca cagctggcca tagcctggtg
     1801 cctgaggaac gagggcgtca gctccgtgct cctgggtgct tccaatgcag aacaacttat
     1861 ggagaacatt ggagcaatac aggtccttcc aaaattgtcg tcctccatcg tccacgagat
     1921 cgacagcatt ctgggcaata aaccctacag caaaaaggac tatagatcct aagtcatcga
     1981 taccgtcaac ttgtttattg cagcttataa tggttacaaa taaagcaata gcatcacaaa
     2041 tttcacaaat aaagcatttt tttcactgca ttctagttgt ggtttgtcca aactcatcaa
     2101 tgtatcttat catgtctgga tccccgggta cccaattcgc cctatagtga gtcgtattac
     2161 aattcactgg ccgtcgtttt acaacgtcgt gactgggaaa accctggcgt tacccaactt
     2221 aatcgccttg cagcacatcc ccctttcgcc agctggcgta atagcgaaga ggcccgcacc
     2281 gatcgccctt cccaacagtt gcgcagcctg aatggcgaat gggacgcgcc ctgtagcggc
     2341 gcattaagcg cggcgggtgt ggtggttacg cgcagcgtga ccgctacact tgccagcgcc
     2401 ctagcgcccg ctcctttcgc tttcttccct tcctttctcg ccacgttcgc cggctttccc
     2461 cgtcaagctc taaatcgggg gctcccttta gggttccgat ttagtgcttt acggcacctc
     2521 gaccccaaaa aacttgatta gggtgatggt tcacgtagtg ggccatcgcc ctgatagacg
     2581 gtttttcgcc ctttgacgtt ggagtccacg ttctttaata gtggactctt gttccaaact
     2641 ggaacaacac tcaaccctat ctcggtctat tcttttgatt tataagggat tttgccgatt
     2701 tcggcctatt ggttaaaaaa tgagctgatt taacaaaaat ttaacgcgaa ttttaacaaa
     2761 atattaacgc ttacaattta ggtggcactt ttcggggaaa tgtgcgcgga acccctattt
     2821 gtttattttt ctaaatacat tcaaatatgt atccgctcat gagacaataa ccctgataaa
     2881 tgcttcaata atattgaaaa aggaagagta tgagtattca acatttccgt gtcgccctta
     2941 ttcccttttt tgcggcattt tgccttcctg tttttgctca cccagaaacg ctggtgaaag
     3001 taaaagatgc tgaagatcag ttgggtgcac gagtgggtta catcgaactg gatctcaaca
     3061 gcggtaagat ccttgagagt tttcgccccg aagaacgttt tccaatgatg agcactttta
     3121 aagttctgct atgtggcgcg gtattatccc gtattgacgc cgggcaagag caactcggtc
     3181 gccgcataca ctattctcag aatgacttgg ttgagtactc accagtcaca gaaaagcatc
     3241 ttacggatgg catgacagta agagaattat gcagtgctgc cataaccatg agtgataaca
     3301 ctgcggccaa cttacttctg acaacgatcg gaggaccgaa ggagctaacc gcttttttgc
     3361 acaacatggg ggatcatgta actcgccttg atcgttggga accggagctg aatgaagcca
     3421 taccaaacga cgagcgtgac accacgatgc ctgtagcaat ggcaacaacg ttgcgcaaac
     3481 tattaactgg cgaactactt actctagctt cccggcaaca attaatagac tggatggagg
     3541 cggataaagt tgcaggacca cttctgcgct cggcccttcc ggctggctgg tttattgctg
     3601 ataaatctgg agccggtgag cgtgggtctc gcggtatcat tgcagcactg gggccagatg
     3661 gtaagccctc ccgtatcgta gttatctaca cgacggggag tcaggcaact atggatgaac
     3721 gaaatagaca gatcgctgag ataggtgcct cactgattaa gcattggtaa ctgtcagacc
     3781 aagtttactc atatatactt tagattgatt taaaacttca tttttaattt aaaaggatct
     3841 aggtgaagat cctttttgat aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc
     3901 actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc
     3961 gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg
     4021 atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa
     4081 atactgttct tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc
     4141 ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt
     4201 gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa
     4261 cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc
     4321 tacagcgtga gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc
     4381 cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct
     4441 ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat
     4501 gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc
     4561 tggccttttg ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg
     4621 ataaccgtat taccgccttt gagtgagctg ataccgctcg ccgcagccga acgaccgagc
     4681 gcagcgagtc agtgagcgag gaagcggaag agcgcccaat acgcaaaccg cctctccccg
     4741 cgcgttggcc gattcattaa tgcagctggc acgacaggtt tcccgactgg aaagcgggca
     4801 gtgagcgcaa cgcaattaat gtgagttagc tcactcatta ggcaccccag gctttacact
     4861 ttatgcttcc ggctcgtatg ttgtgtgga
//
