LOCUS       sequence-394747-       10579 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Multiplexed CRISPR array expressing 7 sgRNA in a lentiviral 
            backbone.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10579)
  AUTHORS   Teh SSK, Bowland K, Halper-Stromberg E, Kotwal A, Bennett A, Skaist 
            A, Tang J, Cai F, Macoretta A, Liang H, Kamiyama H, Wheelan S, Lin 
            MT, Hruban RH, Hung CF, Goldstein M, Scharpf RB, Roberts NJ, 
            Eshleman JR
  TITLE     CRISPR-Cas9 for selective targeting of somatic mutations in 
            pancreatic cancers.
  JOURNAL   NAR Cancer. 2024 Jun 19;6(2):zcae028. doi: 10.1093/narcan/zcae028. 
            eCollection 2024 Jun.
  PUBMED    38915758
REFERENCE   2  (bases 1 to 10579)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "NAR 
            Cancer."; date: "2024-06-19"; volume: "6(2):zcae028. doi"; pages: " 
            10.1093/narcan/zcae028. eCollection 2024 Jun"
COMMENT     Sequence Label: lentiGuide-puro_Panc480-MT7
FEATURES             Location/Qualifiers
     source          1..10579
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     155..174
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(393..497)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(523..978)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(660..681)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     872..891
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     1105..1127
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     1119..1136
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     1120..1136
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     1146..1165
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        1146..1164
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     rep_origin      1185..1320
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     polyA_signal    1360..1481
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(1371..1390)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     1425..1444
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     LTR             1559..1792
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     misc_feature    1864..2452
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     CDS             1970..1981
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     2379..2399
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(2468..3067)
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(2551..2571)
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     primer_bind     3048..3067
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     promoter        complement(3080..4258)
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          3089..4027
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     complement(3112..3132)
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     misc_feature    4397..4514
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     misc_RNA        complement(4570..4645)
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     promoter        complement(4667..4915)
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     misc_RNA        complement(4950..5025)
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     promoter        complement(5047..5295)
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     misc_RNA        complement(5330..5405)
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     promoter        complement(5427..5675)
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     misc_RNA        complement(5691..5766)
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     promoter        complement(5788..6036)
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     misc_RNA        complement(6102..6177)
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     promoter        complement(6199..6447)
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     misc_RNA        complement(6463..6538)
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     promoter        complement(6560..6808)
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     misc_RNA        complement(6823..6898)
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     promoter        complement(6920..7168)
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     CDS             complement(7349..7393)
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    7578..7811
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     misc_feature    8304..8429
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     LTR             8476..8656
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     promoter        complement(8657..8883)
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     primer_bind     complement(8911..8931)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(8911..8929)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(8950..8966)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8950..8966)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(8963..8985)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    8974..8990
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(8998..9004,9005..9022,9023..9028))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9043..9064
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(9181..9198)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(9352..9940)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(9432..9451)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(10111..10579,1..323,324..392))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
ORIGIN
        1 gtgttatcac tcatggttat ggcagcactg cataattctc ttactgtcat gccatccgta
       61 agatgctttt ctgtgactgg tgagtactca accaagtcat tctgagaata gtgtatgcgg
      121 cgaccgagtt gctcttgccc ggcgtcaata cgggataata ccgcgccaca tagcagaact
      181 ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa aactctcaag gatcttaccg
      241 ctgttgagat ccagttcgat gtaacccact cgtgcaccca actgatcttc agcatctttt
      301 actttcacca gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga
      361 ataagggcga cacggaaatg ttgaatactc atactcttcc tttttcaata ttattgaagc
      421 atttatcagg gttattgtct catgagcgga tacatatttg aatgtattta gaaaaataaa
      481 caaatagggg ttccgcgcac atttccccga aaagtgccac ctaaattgta agcgttaata
      541 ttttgttaaa attcgcgtta aatttttgtt aaatcagctc attttttaac caataggccg
      601 aaatcggcaa aatcccttat aaatcaaaag aatagaccga gatagggttg agtgttgttc
      661 cagtttggaa caagagtcca ctattaaaga acgtggactc caacgtcaaa gggcgaaaaa
      721 ccgtctatca gggcgatggc ccactacgtg aaccatcacc ctaatcaagt tttttggggt
      781 cgaggtgccg taaagcacta aatcggaacc ctaaagggag cccccgattt agagcttgac
      841 ggggaaagcc ggcgaacgtg gcgagaaagg aagggaagaa agcgaaagga gcgggcgcta
      901 gggcgctggc aagtgtagcg gtcacgctgc gcgtaaccac cacacccgcc gcgcttaatg
      961 cgccgctaca gggcgcgtcc cattcgccat tcaggctgcg caactgttgg gaagggcgat
     1021 cggtgcgggc ctcttcgcta ttacgccagc tggcgaaagg gggatgtgct gcaaggcgat
     1081 taagttgggt aacgccaggg ttttcccagt cacgacgttg taaaacgacg gccagtgagc
     1141 gcgcgtaata cgactcacta tagggcgaat tgggtacgtc cctaggcctc caaaaaagcc
     1201 tcctcactac ttctggaata gctcagaggc cgaggcggcc tcggcctctg cataaataaa
     1261 aaaaattagt cagccatggg gcggagaatg ggcggaactg ggcggagtta ggggcgggat
     1321 gggcggagtt aggggcggga tagctagagc cagacatgat aagatacatt gatgagtttg
     1381 gacaaaccac aactagaatg cagtgaaaaa aatgctttat ttgtgaaatt tgtgatgcta
     1441 ttgctttatt tgtaaccatt ataagctgca ataaacaagt tcctctcact ctctgatatt
     1501 catttctttg caagttataa atactgaata ataagatgac atgaactact atacgtactg
     1561 ctagagattt tccacactga ctaaaagggt ctgagggatc tctagttacc agagtcacac
     1621 aacagacggg cacacactac ttgaagcact caaggcaagc tttattgagg cttaagcagt
     1681 gggttcccta gttagccaga gagctcccag gctcagatct ggtctaacca gagagaccca
     1741 gtacaagcaa aaagcagatc ttgtcttcgt tgggagtgaa ttagcccttc cagtcccccc
     1801 ttttctttta aaaagtggct aagatctaca gctgccttgt aagtcattgg tcttaaagtc
     1861 gacgcgggga ggcggcccaa agggagatcc gactcgtctg agggcgaagg cgaagacgcg
     1921 gaagaggccg cagagccggc agcaggccgc gggaaggaag gtccgctgga ttgagggccg
     1981 aagggacgta gcagaaggac gtcccgcgca gaatccaggt ggcaacacag gcgagcagcc
     2041 aaggaaagga cgatgatttc cccgacaaca ccacggaatt gtcagtgccc aacagccgag
     2101 cccctgtcca gcagcgggca aggcaggcgg cgatgagttc cgccgtggca atagggaggg
     2161 ggaaagcgaa agtcccggaa aggagctgac aggtggtggc aatgccccaa ccagtggggg
     2221 ttgcgtcagc aaacacagtg cacaccacgc cacgttgcct gacaacgggc cacaactcct
     2281 cataaagaga cagcaaccag gatttataca aggaggagaa aatgaaagcc atacgggaag
     2341 caatagcatg atacaaaggc attaaagcag cgtatccaca tagcgtaaaa ggagcaacat
     2401 agttaagaat accagtcaat ctttcacaaa ttttgtaatc cagaggttga ttgtcgactt
     2461 aacgcgttca ggcaccgggc ttgcgggtca tgcaccaggt gcgcggtcct tcgggcacct
     2521 cgacgtcggc ggtgacggtg aagccgagcc gctcgtagaa ggggaggttg cggggcgcgg
     2581 aggtctccag gaaggcgggc accccggcgc gctcggccgc ctccactccg gggagcacga
     2641 cggcgctgcc cagacccttg ccctggtggt cgggcgagac tccgacggtg gccaggaacc
     2701 acgcgggctc cttgggccgg tgcggcgcca ggaggccttc catctgttgc tgcgcggcca
     2761 gccgggaacc gctcaactcg gccatgcgcg ggccgatctc ggcgaacacc gcccccgctt
     2821 cgacgctctc cggcgtggtc cagaccgcca cggcggcgcc gtcgtccgcg acccacacct
     2881 tgccgatgtc gagcccgacg cgcgtgagga agagttcttg cagctcggtg acccgctcga
     2941 tgtggcggtc cggatcgacg gtgtggcgcg tggcggggta gtcggcgaac gcggcggcga
     3001 gggtgcgtac ggccctgggg acgtcgtcgc gggtggcgag gcgcaccgtg ggcttgtact
     3061 cggtcatggt ggccgtacgt cacgacacct gaaatggaag aaaaaaactt tgaaccactg
     3121 tctgaggctt gagaatgaac caagatccaa actcaaaaag ggcaaattcc aaggagaatt
     3181 acatcaagtg ccaagctggc ctaacttcag tctccaccca ctcagtgtgg ggaaactcca
     3241 tcgcataaaa cccctccccc caacctaaag acgacgtact ccaaaagctc gagaactaat
     3301 cgaggtgcct ggacggcgcc cggtactccg tggagtcaca tgaagcgacg gctgaggacg
     3361 gaaaggccct tttcctttgt gtgggtgact cacccgcccg ctctcccgag cgccgcgtcc
     3421 tccattttga gctccctgca gcagggccgg gaagcggcca tctttccgct cacgcaactg
     3481 gtgccgaccg ggccagcctt gccgcccagg gcggggcgat acacggcggc gcgaggccag
     3541 gcaccagagc aggccggcca gcttgagact acccccgtcc gattctcggt ggccgcgctc
     3601 gcaggccccg cctcgccgaa catgtgcgct gggacgcacg ggccccgtcg ccgcccgcgg
     3661 ccccaaaaac cgaaatacca gtgtgcagat cttggcccgc atttacaaga ctatcttgcc
     3721 agaaaaaaag cgtcgcagca ggtcatcaaa aattttaaat ggctagagac ttatcgaaag
     3781 cagcgagaca ggcgcgaagg tgccaccaga ttcgcacgcg gcggccccag cgcccaggcc
     3841 aggcctcaac tcaagcacga ggcgaagggg ctccttaagc gcaaggcctc gaactctccc
     3901 acccacttcc aacccgaagc tcgggatcaa gaatcacgta ctgcagccag gtggaagtaa
     3961 ttcaaggcac gcaagggcca taacccgtaa agaggccagg cccgcgggaa ccacacacgg
     4021 cacttacctg tgttctggcg gcaaacccgt tgcgaaaaag aacgttcacg gcgactactg
     4081 cacttatata cggttctccc ccaccctcgg gaaaaaggcg gagccagtac acgacatcac
     4141 tttcccagtt taccccgcgc caccttctct aggcaccgga tcaattgccg acccctcccc
     4201 ccaacttctc ggggactgtg ggcgatgtgc gctctgccca ctgacgggca ccggagccaa
     4261 ttcccactcc tttcaagacc tagaaggtcc attagctgca aagattcctc tctgtttaaa
     4321 actttatcca tctttgcacc cgggccccct cgagccggcg ccaaagtgga tctctgctgt
     4381 ccctgtaata aacccgaaaa ttttgaattt ttgtaatttg tttttgtaat tctttagttt
     4441 gtatgtctgt tgctattatg tctactattc tttcccctgc actgtacccc ccaatccccc
     4501 cttttctttt aaaattgtgg atgaatactg ccatttgtct caagatctag ttacgccaag
     4561 cttaaaaaag caccgactcg gtgccacttt ttcaagttga taacggacta gccttatttt
     4621 aacttgctat ttctagctct aaaacatacg ctcttggccc cctaacggtg tttcgtcctt
     4681 tccacaagat atataaagcc aagaaatcta aatactttca agttacggta agcatatgat
     4741 agtccatttt aaaacataat tttaaaactg caaactaccc aagaaattat tactttctac
     4801 gtcacgtatt ttgtactaat atctttgtgt ttacagtcaa attaattcta attatctctc
     4861 taacagcctt gtatcgtata tgcaaatatg aaggaatcat gggaaatagg ccctcttcct
     4921 gcccgacctt aaaaaagcac cgaaaaaaag caccgactcg gtgccacttt ttcaagttga
     4981 taacggacta gccttatttt aacttgctat ttctagctct aaaacaaggg agataagatg
     5041 tgcaccggtg tttcgtcctt tccacaagat atataaagcc aagaaatcta aatactttca
     5101 agttacggta agcatatgat agtccatttt aaaacataat tttaaaactg caaactaccc
     5161 aagaaattat tactttctac gtcacgtatt ttgtactaat atctttgtgt ttacagtcaa
     5221 attaattcta attatctctc taacagcctt gtatcgtata tgcaaatatg aaggaatcat
     5281 gggaaatagg ccctcttcct gcccgacctt aaaaaagcac cgaaaaaaag caccgactcg
     5341 gtgccacttt ttcaagttga taacggacta gccttatttt aacttgctat ttctagctct
     5401 aaaactgtca tggtttagac acctccggtg tttcgtcctt tccacaagat atataaagcc
     5461 aagaaatcta aatactttca agttacggta agcatatgat agtccatttt aaaacataat
     5521 tttaaaactg caaactaccc aagaaattat tactttctac gtcacgtatt ttgtactaat
     5581 atctttgtgt ttacagtcaa attaattcta attatctctc taacagcctt gtatcgtata
     5641 tgcaaatatg aaggaatcat gggaaatagg ccctctctgg gtcgaaaaaa gcaccgactc
     5701 ggtgccactt tttcaagttg ataacggact agccttattt taacttgcta tttctagctc
     5761 taaaacagct ttcagtcata cattagcggt gtttcgtcct ttccacaaga tatataaagc
     5821 caagaaatcg aaatactttc aagttacggt aagcatatga tagtccattt taaaacataa
     5881 ttttaaaact gcaaactacc caagaaatta ttactttcta cgtcacgtat tttgtactaa
     5941 tatctttgtg tttacagtca aattaattct aattatctct ctaacagcct tgtatcgtat
     6001 atgcaaatat gaaggaatca tgggaaatag gccctctctg ggtcccctcg gggttgggag
     6061 gtgggtctga ctcaagatct agttacgcca agcttaaaaa agcaccgact cggtgccact
     6121 ttttcaagtt gataacggac tagccttatt ttaacttgct atttctagct ctaaaacaca
     6181 actgtgaaga tgattcccgg tgtttcgtcc tttccacaag atatataaag ccaagaaatc
     6241 gaaatacttt caagttacgg taagcatatg atagtccatt ttaaaacata attttaaaac
     6301 tgcaaactac ccaagaaatt attactttct acgtcacgta ttttgtacta atatctttgt
     6361 gtttacagtc aaattaattc taattatctc tctaacagcc ttgtatcgta tatgcaaata
     6421 tgaaggaatc atgggaaata ggccctctct gggtcgaaaa aagcaccgac tcggtgccac
     6481 tttttcaagt tgataacgga ctagccttat tttaacttgc tatttctagc tctaaaacgt
     6541 tagaggtggc aggatattcg gtgtttcgtc ctttccacaa gatatataaa gccaagaaat
     6601 cgaaatactt tcaagttacg gtaagcatat gatagtccat tttaaaacat aattttaaaa
     6661 ctgcaaacta cccaagaaat tattactttc tacgtcacgt attttgtact aatatctttg
     6721 tgtttacagt caaattaatt ctaattatct ctctaacagc cttgtatcgt atatgcaaat
     6781 atgaaggaat catgggaaat aggccctctc tgggtcaaaa aagcaccgac tcggtgccac
     6841 tttttcaagt tgataacgga ctagccttat tttaacttgc tatttctagc tctaaaactc
     6901 cacctttgac tggactgacg gtgtttcgtc ctttccacaa gatatataaa gccaagaaat
     6961 cgaaatactt tcaagttacg gtaagcatat gatagtccat tttaaaacat aattttaaaa
     7021 ctgcaaacta cccaagaaat tattactttc tacgtcacgt attttgtact aatatctttg
     7081 tgtttacagt caaattaatt ctaattatct ctctaacagc cttgtatcgt atatgcaaat
     7141 atgaaggaat catgggaaat aggccctctc tgggtcccct cggggttggg aggtgggtct
     7201 gaaacgacga taatggtgaa tatccctgcc taactctatt cactatagaa agtacagcaa
     7261 aaactattct taaacctacc aagcctccta ctatcattat gaataatttt atataccaca
     7321 gccaatttgt tatgttaaac caattccaca aacttgccca tttatctaat tccaataatt
     7381 cttgttcatt cttttcttgc tggttttgcg attcttcaat taaggagtgt attaagcttg
     7441 tgtaattgtt aatttctctg tcccactcca tccaggtcgt gtgattccaa atctgttcca
     7501 gagatttatt actccaacta gcattccaag gcacagcagt ggtgcaaatg agttttccag
     7561 agcaacccca aatccccagg agctgttgat cctttaggta tctttccaca gccaggattc
     7621 ttgcctggag ctgcttgatg ccccagactg tgagttgcaa cagatgctgt tgcgcctcaa
     7681 tagccctcag caaattgttc tgctgctgca ctataccaga caataattgt ctggcctgta
     7741 ccgtcagcgt cattgacgct gcgcccatag tgcttcctgc tgctcccaag aacccaagga
     7801 acaaagctcc tattcccact gctctttttt ctctctgcac cactcttctc tttgccttgg
     7861 tgggtgctac tcctaatggt tcaattttta ctactttata tttatataat tcacttctcc
     7921 aattgtccct catatctcct cctccaggtc tgaagatcag cggccgcttg ctgtgcggtg
     7981 gtcttacttt tgttttgctc ttcctctatc ttgtctaaag cttccttggt gtcttttatc
     8041 tctatccttt gatgcacaca atagagggtt gctactgtat tatataatga tctaagttct
     8101 tctgatcctg tctgaaggga tggttgtagc tgtcccagta tttgtctaca gccttctgat
     8161 gtttctaaca ggccaggatt aactgcgaat cgttctagct ccctgcttgc ccatactata
     8221 tgttttaatt tatatttttt ctttccccct ggccttaacc gaattttttc ccatcgcgat
     8281 ctaattctcc cccgcttaat actgacgctc tcgcacccat ctctctcctt ctagcctccg
     8341 ctagtcaaaa tttttggcgt actcaccagt cgccgcccct cgcctcttgc cgtgcgcgct
     8401 tcagcaagcc gagtcctgcg tcgagagagc tcctctggtt tccctttcgc tttcaagtcc
     8461 ctgttcgggc gccactgcta gagattttcc acactgacta aaagggtctg agggatctct
     8521 agttaccaga gtcacacaac agacgggcac acactacttg aagcactcaa ggcaagcttt
     8581 attgaggctt aagcagtggg ttccctagtt agccagagag ctcccaggct cagatctggt
     8641 ctaaccagag agacccgttt atgtatcgag ctaggcactt aaatacaata tctctgcaat
     8701 gcggcaattc agtggttcgt ccaatccatg tcagacccgt ctgttgcctt cctaataagg
     8761 cacgatcgta ccaccttact tccaccaatc ggcatgcacg gtgctttttc tctccttgta
     8821 aggcatgttg ctaactcatc gttaccatgt tgcaagacta caagagtatt gcataagact
     8881 acattaagct tgcagctcca gcttttgttc cctttagtga gggttaattg cgcgcttggc
     8941 gtaatcatgg tcatagctgt ttcctgtgtg aaattgttat ccgctcacaa ttccacacaa
     9001 catacgagcc ggaagcataa agtgtaaagc ctggggtgcc taatgagtga gctaactcac
     9061 attaattgcg ttgcgctcac tgcccgcttt ccagtcggga aacctgtcgt gccagctgca
     9121 ttaatgaatc ggccaacgcg cggggagagg cggtttgcgt attgggcgct cttccgcttc
     9181 ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat cagctcactc
     9241 aaaggcggta atacggttat ccacagaatc aggggataac gcaggaaaga acatgtgagc
     9301 aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag
     9361 gctccgcccc cctgacgagc atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc
     9421 gacaggacta taaagatacc aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt
     9481 tccgaccctg ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct
     9541 ttctcatagc tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg
     9601 ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc ttatccggta actatcgtct
     9661 tgagtccaac ccggtaagac acgacttatc gccactggca gcagccactg gtaacaggat
     9721 tagcagagcg aggtatgtag gcggtgctac agagttcttg aagtggtggc ctaactacgg
     9781 ctacactaga agaacagtat ttggtatctg cgctctgctg aagccagtta ccttcggaaa
     9841 aagagttggt agctcttgat ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt
     9901 ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc
     9961 tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt
    10021 atcaaaaagg atcttcacct agatcctttt aaattaaaaa tgaagtttta aatcaatcta
    10081 aagtatatat gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg aggcacctat
    10141 ctcagcgatc tgtctatttc gttcatccat agttgcctga ctccccgtcg tgtagataac
    10201 tacgatacgg gagggcttac catctggccc cagtgctgca atgataccgc gagacccacg
    10261 ctcaccggct ccagatttat cagcaataaa ccagccagcc ggaagggccg agcgcagaag
    10321 tggtcctgca actttatccg cctccatcca gtctattaat tgttgccggg aagctagagt
    10381 aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc attgctacag gcatcgtggt
    10441 gtcacgctcg tcgtttggta tggcttcatt cagctccggt tcccaacgat caaggcgagt
    10501 tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc cgatcgttgt
    10561 cagaagtaag ttggccgca
//
