LOCUS       sequence-394751-         564 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  For protein expression. Human DNPH1 is an N-glycosidase with 
            specificity toward 5-hydroxymethyl uridine monophosphate. Pet28a 
            plasmid, KAN resistant, N-terminal TEV-cleavable 6xHis tag..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 564)
  AUTHORS   Wagner AG, Eskandari R, Schramm VL
  TITLE     An enzyme-coupled microplate assay for activity and inhibition of 
            hmdUMP hydrolysis by DNPH1.
  JOURNAL   Anal Biochem. 2023 Jul 1;672:115171. doi: 10.1016/j.ab.2023.115171. 
            Epub 2023 May 3.
  PUBMED    37142196
REFERENCE   2  (bases 1 to 564)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Anal 
            Biochem."; date: "2023-07-1"; volume: "672:115171. doi"; pages: " 
            10.1016/j.ab.2023.115171. Epub 2023 May 3"
COMMENT     Sequence Label: His-DNPH1
FEATURES             Location/Qualifiers
     source          1..564
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             1..18
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             19..39
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
ORIGIN
        1 catcaccacc accaccatga aaatctatac ttccaatcca tggctgcggc tatggttccg
       61 ggccgcagcg aaagctggga aagaggcgag ccgggtagac cggcactcta cttctgcggc
      121 agcattcgtg gcggccgcga agatcgtacc ctgtacgagc gcatcgtgtc tcgtctgcgt
      181 cgttttggta ctgtgttgac cgaacatgtg gctgcggcgg agctgggtgc gcgtggcgag
      241 gaagcggcgg gtggtgatcg tcttatccac gaacaagacc tggaatggct gcagcaggcg
      301 gacgttgtag ttgccgaggt cacccagccg tcgctgggcg ttggttatga gctgggtcgc
      361 gcagtcgcgt ttaacaaacg tatcctgtgt ctatttcgtc cacagtccgg tcgcgtgttg
      421 tctgcaatga ttcgcggtgc agccgacggc agccgtttcc aggtttggga ttatgaagag
      481 ggtgaggtgg aagcgttgct ggaccgttat ttcgaggctg acccgcctgg tcaagtggcc
      541 gcgagcccgg atccgacgac ctaa
//
