LOCUS       sequence-394761-         819 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  For protein expression. E. coli 2-deoxy-D-ribose-5-phosphate 
            aldolase. Converts dR5P to G3P and acetaldehyde. Pet28a plasmid, KAN
            resistant, N-terminal TEV-cleavable 6xHis tag..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 819)
  AUTHORS   Wagner AG, Eskandari R, Schramm VL
  TITLE     An enzyme-coupled microplate assay for activity and inhibition of 
            hmdUMP hydrolysis by DNPH1.
  JOURNAL   Anal Biochem. 2023 Jul 1;672:115171. doi: 10.1016/j.ab.2023.115171. 
            Epub 2023 May 3.
  PUBMED    37142196
REFERENCE   2  (bases 1 to 819)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Anal 
            Biochem."; date: "2023-07-1"; volume: "672:115171. doi"; pages: " 
            10.1016/j.ab.2023.115171. Epub 2023 May 3"
COMMENT     Sequence Label: His-DERA
FEATURES             Location/Qualifiers
     source          1..819
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             1..18
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             19..39
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
ORIGIN
        1 catcaccacc accaccacga gaacctgtac ttccagagca tgaccgatct gaaagcgagc
       61 agcctgcgtg cgctgaagct gatggatctg accaccctga acgacgatga caccgacgaa
      121 aaagtgattg cgctgtgcca ccaagcgaag accccggttg gtaacaccgc ggcgatctgc
      181 atttatccgc gtttcatccc gattgcgcgt aagaccctga aagagcaagg caccccggaa
      241 atccgtattg cgaccgtgac caactttccg cacggtaacg atgacatcga tattgcgctg
      301 gcggagaccc gtgcggcgat tgcgtacggc gcggatgaag ttgacgtggt tttcccgtat
      361 cgtgcgctga tggcgggcaa cgagcaagtg ggttttgacc tggttaaggc gtgcaaagaa
      421 gcgtgcgcgg cggcgaacgt gctgctgaaa gttatcattg agaccggtga actgaaggat
      481 gaggcgctga tccgtaaagc gagcgaaatc agcattaagg cgggcgcgga cttcattaag
      541 accagcaccg gtaaagttgc ggttaacgcg accccggaga gcgcgcgtat catgatggaa
      601 gtgattcgtg atatgggcgt ggagaagacc gttggtttta agccggcggg tggcgttcgt
      661 accgcggagg atgcgcaaaa gtacctggcg atcgcggacg agctgtttgg tgcggattgg
      721 gcggatgcgc gtcactatcg ttttggtgcg agcagcctgc tggcgagcct gctgaaagcg
      781 ctgggtcatg gtgatggcaa gagcgcgagc agctactaa
//
