LOCUS       sequence-394764-         780 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  For protein expression. A. gambiae deoxynucleoside kinase. 
            Phosphorylates a wide variety of nucleosides at the 5-hydroxyl 
            position. Pet28a plasmid, KAN resistant, N-terminal TEV-cleavable 
            6xHis tag..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 780)
  AUTHORS   Wagner AG, Eskandari R, Schramm VL
  TITLE     An enzyme-coupled microplate assay for activity and inhibition of 
            hmdUMP hydrolysis by DNPH1.
  JOURNAL   Anal Biochem. 2023 Jul 1;672:115171. doi: 10.1016/j.ab.2023.115171. 
            Epub 2023 May 3.
  PUBMED    37142196
REFERENCE   2  (bases 1 to 780)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Anal 
            Biochem."; date: "2023-07-1"; volume: "672:115171. doi"; pages: " 
            10.1016/j.ab.2023.115171. Epub 2023 May 3"
COMMENT     Sequence Label: His-AgdNK
FEATURES             Location/Qualifiers
     source          1..780
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             1..18
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             19..39
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
ORIGIN
        1 caccaccacc accaccacga aaacctgtac ttccagagca tgccgccaat cgcgagcgag
       61 aagctgggtg cgagcggcaa gaaaccgttc accgtgtttg ttgagggtaa catcggtagc
      121 ggcaagacca cctttctgaa ccacttccaa aaatttaacg acatttgcct gctgaccgag
      181 ccggtggaaa agtggcgtaa ctgcggtggc gttaacctgc tggatctgat gtacaaagag
      241 agccaccgtt gggcgatgcc gttccagacc tatgtgaccc tgaccatgct ggacatgcac
      301 acctgccaaa ccgataagag cgtgaaactg atggaacgta gcctgttcag cgcgcgtaac
      361 tgctttgttg agagcatgct ggcgagcggt agcctgcacc agggcatgta caacgtgctg
      421 caagaatggt atgactttat ctgctgcaac atccacattc aggcggatct gattgtttac
      481 ctgcaaacca gcccggaagt ggtttatgag cgtatgaagc agcgtgcgcg tagcgaggaa
      541 agctgcgttc cgctggagta cctgaaagag ctgcacgaac tgcacgagaa ctggctgatt
      601 catggtgcga gcccgcgtcc ggcgccggtg ctggttctga acgcggacct ggatctgaac
      661 accatcggcg cggagtatga acgtagcgaa accagcattc tgaaaccgat cctgattgag
      721 aacaccaacc aacacgcgat tctgaccagc ccggcgaagc gtgcgaaaac cgacttctaa
//
