LOCUS       sequence-394765-        4996 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Contains RBOHD promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4996)
  AUTHORS   Calabria J, Wang L, Rast-Somssich MI, Chen HW, Watt M, Persson S, 
            Andersen TG, Idnurm A, Somssich M
  TITLE     Resolving spatially distinct phytohormone response zones in 
            Arabidopsis thaliana roots colonized by Fusarium oxysporum.
  JOURNAL   J Exp Bot. 2025 Jan 17:erae516. doi: 10.1093/jxb/erae516.
  PUBMED    39823275
REFERENCE   2  (bases 1 to 4996)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Exp Bot. 
            2025 Jan 17:erae516. doi: 10.1093/jxb/erae516."
COMMENT     Sequence Label: pGG-PIP31
FEATURES             Location/Qualifiers
     source          1..4996
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(10..28)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        96..200
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(201..269,270..1061)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(419..438)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      1232..1820
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1721..1740
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     1974..1991
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    2108..2129
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(2144..2149,2150..2167,2168..2174)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2182..2198
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        2212..2230
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     2212..2229
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(4582..4601)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(4583..4601)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(4611..4633)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     complement(4827..4846)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     4946..4968
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
ORIGIN
        1 gacgaaaggg cctcgtgata cgcctatttt tataggttaa tgtcatgata ataatggttt
       61 cttagacgtc aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt
      121 tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat
      181 aatattgaaa aaggaagagt atgagtattc aacatttccg tgtcgccctt attccctttt
      241 ttgcggcatt ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg
      301 ctgaagatca gttgggtgca cgagtgggtt acatcgaact ggatctcaac agcggtaaga
      361 tccttgagag ttttcgcccc gaagaacgtt ttccaatgat gagcactttt aaagttctgc
      421 tatgtggcgc ggtattatcc cgtattgacg ccgggcaaga gcaactcggt cgccgcatac
      481 actattctca gaatgacttg gttgagtact caccagtcac agaaaagcat cttacggatg
      541 gcatgacagt aagagaatta tgcagtgctg ccataaccat gagtgataac actgcggcca
      601 acttacttct gacaacgatc ggaggaccga aggagctaac cgcttttttg cacaacatgg
      661 gggatcatgt aactcgcctt gatcgttggg aaccggagct gaatgaagcc ataccaaacg
      721 acgagcgtga caccacgatg cctgtagcaa tggcaacaac gttgcgcaaa ctattaactg
      781 gcgaactact tactctagct tcccggcaac aattaataga ctggatggag gcggataaag
      841 ttgcaggacc acttctgcgc tcggcccttc cggctggctg gtttattgct gataaatctg
      901 gagccggtga gcgtggctct cgaggtatca ttgcagcact ggggccagat ggtaagccct
      961 cccgtatcgt agttatctac acgacgggga gtcaggcaac tatggatgaa cgaaatagac
     1021 agatcgctga gataggtgcc tcactgatta agcattggta actgtcagac caagtttact
     1081 catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga
     1141 tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt
     1201 cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct
     1261 gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc
     1321 taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgttc
     1381 ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc
     1441 tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg
     1501 ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt
     1561 cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg
     1621 agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg
     1681 gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt
     1741 atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag
     1801 gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt
     1861 gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta
     1921 ttaccgcctt tgagtgagct gataccgctc gccgcagccg aacgaccgag cgcagcgagt
     1981 cagtgagcga ggaagcggaa gagcgcccaa tacgcaaacc gcctctcccc gcgcgttggc
     2041 cgattcatta atgcagctgg cacgacaggt ttcccgactg gaaagcgggc agtgagcgca
     2101 acgcaattaa tgtgagttag ctcactcatt aggcacccca ggctttacac tttatgcttc
     2161 cggctcgtat gttgtgtgga attgtgagcg gataacaatt tcacacgagc tatttaggtg
     2221 acactataga agtgaagctt ggtctcaacc tgacttgtta aattgctctc ttagtcttat
     2281 cataccttca tctcatatcc attataatca tctttagcaa tactatttta tttttaacta
     2341 cgaaaaataa accttgttac gccgttatta acagtgatca tttttttcgt taaagaaatt
     2401 taacattcaa aaaagataaa gaatgatcac caataagcgt catgatcttc cgtatggtga
     2461 tgataaacat cgtttgcagt tttgttgtat agtacaacgt atatgtagcc gtaacgtgtg
     2521 aatagtgtat ggttagggtc attttatttg tgaactacca caattttgat agcagtggag
     2581 ataagtcaaa ggttcgtctt ctaattaatt aacagcgatt attagactat ctaacttttc
     2641 ttcttttctt attttgataa taactatata tcttctttag ccaccttact ttctcatcgg
     2701 gttctattta aatagggaca aagaaaacaa accatcgatt tgtactatgt gctcaattat
     2761 atcatatgtt ttgtctccat ctatgtatct atttctcttt tccatttggt aaattattta
     2821 agacccttaa actgactttt ctcgtgggcg gcgtcgaagt tttggttaac agacaaaaca
     2881 tctgttcatg atgcctgcat aactctatgt ttttatacta ctcatttgcc ctctatttca
     2941 tgctactctt aaaaatagct aaataaaaat acttcaatat tagaaaaaaa atcttcttga
     3001 tgtcaaaaat acactttaga tatcccctct agttcttgtg atgctaatat attacaaaat
     3061 tgtttatata tatagaaata ttacctctag caatacaaaa atcttgttat atcaattaaa
     3121 tataacacta tccgactagt gtttactaaa ataacacata atttttaatt atgatctttg
     3181 aattttagtt tatgtattac gctttataat tttgttatca aaaactataa aagctcaaaa
     3241 gtgagcatct ataccctaat tccgttttta tgcatacact taggtttatt tttattacga
     3301 gaatctgtgt ttagtatata taaaatagag tttatgaatg actaagttaa atacttcaat
     3361 actaattaaa gtaagtcgta ttgatgcaga caatataaac tttagatatc tccgctagtt
     3421 tgtataatgc taatagattc aaattgtctt tatgtagagg aattacctca aataacataa
     3481 aattttcatt ttattaatta aatctcattt tgtcatttca aaagtcattt taattactgt
     3541 gttatttgtc taaagtatac tccaaacaag aaaccaaagt agaaggtcag tgcccaagag
     3601 acaaataaac ttaagcatgt ccactctttt tagcgatcgg atcgcttatc tgttcttatc
     3661 cattatggaa catgaacgat gttcctttaa gactaccaca ttcaaacgaa aaataagtca
     3721 tcgatgatag actggatagt tatagtttga tgatctagct aattgacttt catatttaca
     3781 taaaacagaa atgtgttttt atcaaaaaag aaaaaagaaa aaacagaaaa tgaacaatac
     3841 atctcacatg gagtttgtac aagaatccat aataattttt tgttcggtac agtaggttag
     3901 atatacattt gaatacagtt tacaagccaa atgttagaag caacatattt atatagtatc
     3961 atcaatatac gatccaaaat gaattataga ttgacttatg gaaaagttag atactatatg
     4021 catgattgcc aaatacattg aaaaataaca atttaagttc ttcaaacaaa agtttataaa
     4081 tgactcggtg tttgacataa ctcggggttg tttttctttg ttaattcaca cacggggttg
     4141 gtgtataaat gacttcggtg tttgaaatgt atgacacggt ataaataaaa aaggtaagga
     4201 agaaatgaag aatagaagaa gaacaaaaaa aaaaaggaaa ttaaaaaaaa gtcaaaataa
     4261 atggtcagta aaggattttg accagaccgg agcggtccat ccatagtttc ccactacctt
     4321 ccctgattta gcacagaaga atggcaattt gcatgccatt tatttcatct attcctcctt
     4381 cactctataa atatatttta aacaaaatca atttcttcca tacacaaaaa tcaaacacct
     4441 tttgagagcg gttatttttt ctctatcaac taatacagta accttacggg tgtttatttg
     4501 tatagatctc tgtggttttc ttggccaaat ctagtgagat ctttttggtt tctcgaattc
     4561 gaacatgaga ccgaattctc gccctatagt gagtcgtatt acctggccgt cgttttacaa
     4621 cgtcgtgact gggaaaaccc tggcgttacc caacttaatc gccttgcagc acatccccct
     4681 ttcgccagct ggcgtaatag cgaagaggcc cgcaccgatc gcccttccca acagttgcgc
     4741 agcctgaatg gcgaatggcg cctgatgcgg tattttctcc ttacgcatct gtgcggtatt
     4801 tcacaccgca tatggtgcac tctcagtaca atctgctctg atgccgcata gttaagccag
     4861 ccccgacacc cgccaacacc cgctgacgcg ccctgacggg cttgtctgct cccggcatcc
     4921 gcttacagac aagctgtgac cgtctccggg agctgcatgt gtcagaggtt ttcaccgtca
     4981 tcaccgaaac gcgcga
//
