LOCUS       sequence-394800-        7238 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Contains RBOHF promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7238)
  AUTHORS   Calabria J, Wang L, Rast-Somssich MI, Chen HW, Watt M, Persson S, 
            Andersen TG, Idnurm A, Somssich M
  TITLE     Resolving spatially distinct phytohormone response zones in 
            Arabidopsis thaliana roots colonized by Fusarium oxysporum.
  JOURNAL   J Exp Bot. 2025 Jan 17:erae516. doi: 10.1093/jxb/erae516.
  PUBMED    39823275
REFERENCE   2  (bases 1 to 7238)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Exp Bot. 
            2025 Jan 17:erae516. doi: 10.1093/jxb/erae516."
COMMENT     Sequence Label: pGG-PIP32
FEATURES             Location/Qualifiers
     source          1..7238
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(10..28)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        96..200
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(201..269,270..1061)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(419..438)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      1232..1820
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1721..1740
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     1974..1991
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    2108..2129
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(2144..2149,2150..2167,2168..2174)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2182..2198
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        2212..2230
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     2212..2229
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(6824..6843)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(6825..6843)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(6853..6875)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     complement(7069..7088)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     7188..7210
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
ORIGIN
        1 gacgaaaggg cctcgtgata cgcctatttt tataggttaa tgtcatgata ataatggttt
       61 cttagacgtc aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt
      121 tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat
      181 aatattgaaa aaggaagagt atgagtattc aacatttccg tgtcgccctt attccctttt
      241 ttgcggcatt ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg
      301 ctgaagatca gttgggtgca cgagtgggtt acatcgaact ggatctcaac agcggtaaga
      361 tccttgagag ttttcgcccc gaagaacgtt ttccaatgat gagcactttt aaagttctgc
      421 tatgtggcgc ggtattatcc cgtattgacg ccgggcaaga gcaactcggt cgccgcatac
      481 actattctca gaatgacttg gttgagtact caccagtcac agaaaagcat cttacggatg
      541 gcatgacagt aagagaatta tgcagtgctg ccataaccat gagtgataac actgcggcca
      601 acttacttct gacaacgatc ggaggaccga aggagctaac cgcttttttg cacaacatgg
      661 gggatcatgt aactcgcctt gatcgttggg aaccggagct gaatgaagcc ataccaaacg
      721 acgagcgtga caccacgatg cctgtagcaa tggcaacaac gttgcgcaaa ctattaactg
      781 gcgaactact tactctagct tcccggcaac aattaataga ctggatggag gcggataaag
      841 ttgcaggacc acttctgcgc tcggcccttc cggctggctg gtttattgct gataaatctg
      901 gagccggtga gcgtggctct cgaggtatca ttgcagcact ggggccagat ggtaagccct
      961 cccgtatcgt agttatctac acgacgggga gtcaggcaac tatggatgaa cgaaatagac
     1021 agatcgctga gataggtgcc tcactgatta agcattggta actgtcagac caagtttact
     1081 catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga
     1141 tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt
     1201 cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct
     1261 gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc
     1321 taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgttc
     1381 ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc
     1441 tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg
     1501 ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt
     1561 cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg
     1621 agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg
     1681 gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt
     1741 atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag
     1801 gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt
     1861 gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta
     1921 ttaccgcctt tgagtgagct gataccgctc gccgcagccg aacgaccgag cgcagcgagt
     1981 cagtgagcga ggaagcggaa gagcgcccaa tacgcaaacc gcctctcccc gcgcgttggc
     2041 cgattcatta atgcagctgg cacgacaggt ttcccgactg gaaagcgggc agtgagcgca
     2101 acgcaattaa tgtgagttag ctcactcatt aggcacccca ggctttacac tttatgcttc
     2161 cggctcgtat gttgtgtgga attgtgagcg gataacaatt tcacacgagc tatttaggtg
     2221 acactataga agtgaagctt ggtctcaacc taccggttga aaataagagt ggtggatcca
     2281 tataaaccaa tcttgattac tcaaaagaaa ccggtttggt tttacaaggg gacataacgg
     2341 cgtcgtagga gtcgtctgta aatacgaatg gtgtcacggt gtaaggtaag ttcttctcta
     2401 tgtggacagt gtttaggtgc agaagactat atatattgta ttgccttcaa tgttgtaaca
     2461 tttttttttt tcagttgtag ttgtagccac acagttcttt ttaaggtctg ttgtgtttaa
     2521 agatttttaa ttctacgagg tcaatgataa aattctcaac ttgagtgtcg tttgtatatt
     2581 ttgggtggga aaggttcgaa ctctcgaccc caatccgctt cttctctgct ctttggctgc
     2641 tgctaaatgc cctgaccgtc tgcttcgaag ttaattgcta ggcataacaa acaagaaaat
     2701 ttttgtgttt gttgaggacg taaaatgcac gtaccacaca tgtgtaatgt actaatgtgg
     2761 ttgtatatga aaataattat tataacctaa gataatatat taaatttgga aagccatcat
     2821 gaagtcggtt ctaattcaaa cttacttaat cgtttatgta tgtggtgtca caccgatcac
     2881 gatcggttag tttgtgtttc tcaagtgttc tattattata tacttttcaa aagatttgtt
     2941 aaatttattg tttgtttaat tggttacttt tgagtttttc gcaaaggaac agcggagatt
     3001 aatatatgag ttctatagcc aacataaatt taatcaacca aattttacca accttttttc
     3061 ttttaaaatg tattctccta tcaaatagta cacgagataa acaattctaa tctatttaaa
     3121 gaaaaaaatg atatttaaaa ccttatacct aatgaaaaca ctttgaacac atgataaatc
     3181 attaataata attttgttct aacttaattc catttattgc tttatttatt tttctatatt
     3241 ttgctgtgaa attaggataa tttttgttga tgtaaagtta ctacaaaaaa atcttgtttg
     3301 tatatcacta ctagagtttg agaatgttag ttaacaacca aagaaaaatg gttgtcttaa
     3361 agggacaatt gttgtatttt cactctaatg aaggagttag gatggtaaag agaacatgac
     3421 aattcaaagt caactaagta actacaataa ataatttcat agatgacata gtttctctcc
     3481 catgtacaag aagtggttgt ttcattgact tggaagatat atggtgggcc catttcacgc
     3541 actatattgg gcctcaatcg cgttattttt cttctagaag cccaaaagtt tttgtgtact
     3601 atacatgcat gaacgtcaaa aattattact attagttcaa cgaatttata ctatggtcta
     3661 tggacttggt gacaaaagta aactatgaaa tactagacaa ttattcgtaa ttgtttttat
     3721 ccgcttgact ttgaatattt cccatatatg ataaattttc attaacttga gtgaataacc
     3781 aaaatctaac taatatataa ttatcggcaa gatatcagat ttttatgtgt aggaaaacca
     3841 aagaagaaga aaacgaacaa accaaaagtt aagacttttc tttactgttc ggtacggtat
     3901 gcttcaagtt ttaatcatcc tacatatatt taagatgtgt gtagtcgtcg agtagtaaca
     3961 cttatcatat tttctaatga atctaaacta tgaaactatt ttgacaaaga atcctaataa
     4021 aaatcgaacc taatcaagaa gaaaacgaat ttgctatatc ataaaaaaat taccactagg
     4081 ccactacttt ctaaacttaa agttaatcca tcatatttcc aaatcgtttg gtaatcaatg
     4141 gatcggataa atgaactgat tagtgattac gtactatata attaacagtc atgaaaagca
     4201 aaccgcaaac gatacgtcaa attttaaaac ttgaaaagtc aagcagtggg ttaactttct
     4261 ttagatttat gaaacaatgt tctatattct aaatagtttc ttacagtaaa attcaaatct
     4321 atatatacac aaatagaaaa atgattggtt tttgtaactt gggttggtag aatattcata
     4381 tcacatttga catttctatt cttcaagaat aacttatggg agaagttact ttcgttcgaa
     4441 aactataaaa gttgcagaag atagtgaaga tagttgcaga attgtattat atatgttata
     4501 cccagttatg tgtgcatgtg tgtacatata tatatgtgta cactaataca cacgtttttc
     4561 tatgaaatac gagtttgttc aaaaacatct gacttttgtt cattcatata aaatgttaaa
     4621 cgaacttttc ctacttttta gtgaatatat ttcgtcaaat tgaaattgat gccaaaatta
     4681 ggtataacgt gaaaaacgta tatgtttttt aaaaatgaga ttagttacgt ttgaaaatca
     4741 aatcttttca catttcactt tcatacttgg agtttttgtt ttgttttata acttgtgtat
     4801 ttactactac aactgtttgc acaacataac attgaattga aatctttata tattaaaatg
     4861 aaaacactca accccttaaa atagaaatgg ttcacattcg agtttggtta ttattttctg
     4921 ataactgaat tagatgccga accaaaacca tacaaatttc cgagagatgt agaatgatgg
     4981 gcattttaat cgtaccaaac agaaccgaac caaaccaaac aaccgaaccg tagtgacgta
     5041 gtctacatct aacccatttg gttaaacttt tttcaaccct aatggttcgg tttgaaccat
     5101 taaccctaac tgttttttaa tttataatta atttcgttag gtagataata ttacatgctt
     5161 gattttggtt atttatcaaa actaagttta cttagttctt tcaatcctta aatctctaat
     5221 tctaagctac aattttataa ggtatggaaa aaactattca tatctcattt ctattccgtt
     5281 acttttcaaa aatttcaagt tttttttgac taagaaaaga ttttataaaa aaaatatggc
     5341 taataaccac aacgtttttc ttgagtatta ttattaatag actaaacaaa tacaaataaa
     5401 gtttacccca ctcgaatata aatatttttg aggaaatgta tctactatct tcttaaacaa
     5461 cgtcgtgtca ttactcaaaa gtgtccaacg ggcaatacaa gattatgatg gacgacccat
     5521 gattaaacat gttcgatcta atctaaaatc tttttagtct atattagtga agcactgaaa
     5581 acaaaatgtt gttatcaaat ttgatttgtt cttgttaaca ataaattctt ttcaaaagtg
     5641 agcaaaatat ttgtagtcgt ggacttaaca acaatacatc aatcgctaat aaaacactgg
     5701 cttccacaaa tgatgtataa aaactatatt caaagtcgtc gtccttgttt tttctctcat
     5761 cagttcacaa acaagctcaa ctttggataa aacgcgtcgg acgtacgaaa aaaagaagac
     5821 ttgcagagaa acaacagtca cacacacaat cacctcagat ttaaaaatat tcctttggta
     5881 ttacttgaac taacgtcatc cgagttgttt ctatatatat gtacatatat tctttacatg
     5941 ttatttatag ctaattggaa gtttttagtg cttattgcta gctactaatt aataacgagt
     6001 ttaattctaa tgaaaattgg acacaaaatc tgatactaag ttgcctatat aagattagaa
     6061 atttaaacta ataacagtta catatataca aaatcgtgtt gtacctcata taattatggt
     6121 gcatttgaat aagatatcta ttgaaaaata tataatgaaa aattaaattg gtactctaat
     6181 caaaaaaaaa aaaaatcttt aacacatcaa atgttcaatt tttttaaaag tccaaatcgt
     6241 tttgatattg ttttttattg aagagcaaac tactataaga aaaatatttg aagaaacata
     6301 gcattttccc atgcataaac tagaatgacc aacaaagata ctctcaaagt ctcacttgtc
     6361 taaaaaacag ataaaaatca tttccaattt tgagaaaagg aaaaaaaaac aactccaata
     6421 atactttttg attttctttt cgttgcatta aaataaatgt ggcaaaaatc caataatatt
     6481 actattataa gttaaaaaaa ctcaaaaaaa aaaaaaagta tattccgtca actttttctc
     6541 agtccggttc aatcatctcc atcatcgttg atctctctct ctccgtcaac gttctctcta
     6601 taaagcagag agtttcacag cgcgtgaaaa atggcttttc ccacttagcc acaacgacgc
     6661 gtttcaattc tccatctctc cctctctctc tctctttctt caaagattcc accaacctat
     6721 acatatactt atatataatc tatgctacgt gtatatactt ccgatatcct tcaaccaact
     6781 ctttgaattc cgactttgga tctaacatga gaccgaattc tcgccctata gtgagtcgta
     6841 ttacctggcc gtcgttttac aacgtcgtga ctgggaaaac cctggcgtta cccaacttaa
     6901 tcgccttgca gcacatcccc ctttcgccag ctggcgtaat agcgaagagg cccgcaccga
     6961 tcgcccttcc caacagttgc gcagcctgaa tggcgaatgg cgcctgatgc ggtattttct
     7021 ccttacgcat ctgtgcggta tttcacaccg catatggtgc actctcagta caatctgctc
     7081 tgatgccgca tagttaagcc agccccgaca cccgccaaca cccgctgacg cgccctgacg
     7141 ggcttgtctg ctcccggcat ccgcttacag acaagctgtg accgtctccg ggagctgcat
     7201 gtgtcagagg ttttcaccgt catcaccgaa acgcgcga
//
