LOCUS       sequence-394835-       10410 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Negative selection blasticidin resistant Tet-inducible Matreyek 
            Bxb1(GA) landing pad.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10410)
  AUTHORS   Roelle SM, Kamath ND, Matreyek KA
  TITLE     Mammalian Genomic Manipulation with Orthogonal Bxb1 DNA Recombinase 
            Sites for the Functional Characterization of Protein Variants.
  JOURNAL   ACS Synth Biol. 2023 Nov 3. doi: 10.1021/acssynbio.3c00355.
  PUBMED    37922210
REFERENCE   2  (bases 1 to 10410)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "ACS Synth 
            Biol. 2023 Nov 3. doi: 10.1021/acssynbio.3c00355."
COMMENT     Sequence Label: 
            G417A_pLenti-Tet-BattP(GA)-BFP-2A-iCasp9-2A-Blast_rtTA3
FEATURES             Location/Qualifiers
     source          1..10410
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             join(1..69,70..861)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(219..238)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      1032..1620
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1521..1540
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     1774..1791
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    1908..1929
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(1944..1949,1950..1967,1968..1974)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    1982..1998
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     1987..2009
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     2006..2022
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     2006..2022
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     2041..2061
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        2043..2061
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        2089..2315
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             2316..2496
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    2543..2668
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    3161..3394
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             3579..3623
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    3890..4006
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        4149..4498
                     /label=TRE3GV promoter
                     /note="3rd-generation Tet-responsive promoter that can be 
                     activated by binding of Tet-On(R) 3G, optimized for 
                     retroviral and lentiviral vectors"
     protein_bind    4157..4175
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    4193..4211
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    4229..4247
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    4265..4283
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    4301..4319
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    4337..4355
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    4373..4391
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    4500..4547
                     /label=Bxb1 attP
                     /bound_moiety="Mycobacteriophage Bxb1 integrase"
                     /note="Minimal attP site for the Mycopbacteriophage Bxb1 
                     integrase (Voutev and Mann, 2017)"
     regulatory      4548..4557
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             4554..5264
                     /codon_start=1
                     /product="enhanced monomeric blue fluorescent protein 
                     (Subach et al., 2011)"
                     /label=mTagBFP2
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELIKENMHMKLYMEGTVDNHHFKCTSEGEGKPYEGTQTM
                     RIKVVEGGPLPFAFDILATSFLYGSKTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGG
                     VLTATQDTSLQDGCLIYNVKIRGVNFTSNGPVMQKKTLGWEAFTETLYPADGGLEGRND
                     MALKLVGGSHLIANAKTTYRSKKPAKNLKMPGVYYVDYRLERIKEANNETYVEQHEVAV
                     ARYCDLPSKLGHKLN"
     CDS             5274..5330
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             5346..5666
                     /codon_start=1
                     /product="F36V mutant of FK506-binding protein FKBP12"
                     /label=DmrB
                     /note="binds synthetic ligands that do not bind wild-type 
                     FKBP"
                     /translation="GVQVETISPGDGRTFPKRGQTCVVHYTGMLEDGKKVDSSRDRNKP
                     FKFMLGKQEVIRGWEEGVAQMSVGQRAKLTISPDYAYGATGHPGIIPPHATLVFDVELL
                     KLE"
     CDS             complement(6018..6029)
                     /codon_start=1
                     /product="WELQut protease recognition and cleavage site"
                     /label=WELQut site
                     /translation="WELQ"
     CDS             6543..6569
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             6588..6641
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             6642..7037
                     /codon_start=1
                     /gene="Aspergillus terreus BSD"
                     /product="blasticidin S deaminase"
                     /label=BSD
                     /note="confers resistance to blasticidin"
                     /translation="AKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTGV
                     NVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGIK
                     AIVKDSDGQPTAVGIRELLPSGYVWEG"
     primer_bind     complement(7130..7149)
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     polyA_signal    7195..7250
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(7249..7268)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     enhancer        7584..7887
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        7888..8091
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     protein_bind    8196..8220
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             8223..8930
                     /codon_start=1
                     /product="reverse tetracycline transactivator 3"
                     /label=rtTA3
                     /note="activates expression from Tet-responsive promoters 
                     in the presence of doxycycline"
                     /translation="MSRLDKSKVINGALELLNGVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALPIEMLDRHHTHFCPLEGESWQDFLRNNAKSYRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEEQEHQVAKEERETPTT
                     DSMPPLLRQAIELFDRQGAEPAFLFGLELIICGLEKQLKCESGGPTDALDDFDLDMLPA
                     DALDDFDLDMLPG"
     LTR             9017..9250
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    9322..9443
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(9359..9378)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     9413..9432
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      9483..9618
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(9638..9657)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(9639..9657)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(9667..9684)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(9667..9683)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(9676..9698)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      9825..10280
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(9912..9931)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     10122..10143
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        10306..10410
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 atgagtattc aacatttccg tgtcgccctt attccctttt ttgcggcatt ttgccttcct
       61 gtttttgctc acccagaaac gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca
      121 cgagtgggtt acatcgaact ggatctcaac agcggtaaga tccttgagag ttttcgcccc
      181 gaagaacgtt ttccaatgat gagcactttt aaagttctgc tatgtggcgc ggtattatcc
      241 cgtattgacg ccgggcaaga gcaactcggt cgccgcatac actattctca gaatgacttg
      301 gttgagtact caccagtcac agaaaagcat cttacggatg gcatgacagt aagagaatta
      361 tgcagtgctg ccataaccat gagtgataac actgcggcca acttacttct gacaacgatc
      421 ggaggaccga aggagctaac cgcttttttg cacaacatgg gggatcatgt aactcgcctt
      481 gatcgttggg aaccggagct gaatgaagcc ataccaaacg acgagcgtga caccacgatg
      541 cctgtagcaa tggcaacaac gttgcgcaaa ctattaactg gcgaactact tactctagct
      601 tcccggcaac aattaataga ctggatggag gcggataaag ttgcaggacc acttctgcgc
      661 tcggcccttc cggctggctg gtttattgct gataaatctg gagccggtga gcgtgggtct
      721 cgcggtatca ttgcagcact ggggccagat ggtaagccct cccgtatcgt agttatctac
      781 acgacgggga gtcaggcaac tatggatgaa cgaaatagac agatcgctga gataggtgcc
      841 tcactgatta agcattggta actgtcagac caagtttact catatatact ttagattgat
      901 ttaaaacttc atttttaatt taaaaggatc taggtgaaga tcctttttga taatctcatg
      961 accaaaatcc cttaacgtga gttttcgttc cactgagcgt cagaccccgt agaaaagatc
     1021 aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa
     1081 ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag
     1141 gtaactggct tcagcagagc gcagatacca aatactgttc ttctagtgta gccgtagtta
     1201 ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta
     1261 ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag
     1321 ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg
     1381 gagcgaacga cctacaccga actgagatac ctacagcgtg agctatgaga aagcgccacg
     1441 cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag
     1501 cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc
     1561 cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa
     1621 aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg
     1681 ttctttcctg cgttatcccc tgattctgtg gataaccgta ttaccgcctt tgagtgagct
     1741 gataccgctc gccgcagccg aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa
     1801 gagcgcccaa tacgcaaacc gcctctcccc gcgcgttggc cgattcatta atgcagctgg
     1861 cacgacaggt ttcccgactg gaaagcgggc agtgagcgca acgcaattaa tgtgagttag
     1921 ctcactcatt aggcacccca ggctttacac tttatgcttc cggctcgtat gttgtgtgga
     1981 attgtgagcg gataacaatt tcacacagga aacagctatg accatgatta cgccaagcgc
     2041 gcaattaacc ctcactaaag ggaacaaaag ctggagctgc aagcttaatg tagtcttatg
     2101 caatactctt gtagtcttgc aacatggtaa cgatgagtta gcaacatgcc ttacaaggag
     2161 agaaaaagca ccgtgcatgc cgattggtgg aagtaaggtg gtacgatcgt gccttattag
     2221 gaaggcaaca gacgggtctg acatggattg gacgaaccac tgaattgccg cattgcagag
     2281 atattgtatt taagtgccta gctcgataca taaacgggtc tctctggtta gaccagatct
     2341 gagcctggga gctctctggc taactaggga acccactgct taagcctcaa taaagcttgc
     2401 cttgagtgct tcaagtagtg tgtgcccgtc tgttgtgtga ctctggtaac tagagatccc
     2461 tcagaccctt ttagtcagtg tggaaaatct ctagcagtgg cgcccgaaca gggacttgaa
     2521 agcgaaaggg aaaccagagg agctctctcg acgcaggact cggcttgctg aagcgcgcac
     2581 ggcaagaggc gaggggcggc gactggtgag tacgccaaaa attttgacta gcggaggcta
     2641 gaaggagaga gatgggtgcg agagcgtcag tattaagcgg gggagaatta gatcgcgatg
     2701 ggaaaaaatt cggttaaggc cagggggaaa gaaaaaatat aaattaaaac atatagtatg
     2761 ggcaagcagg gagctagaac gattcgcagt taatcctggc ctgttagaaa catcagaagg
     2821 ctgtagacaa atactgggac agctacaacc atcccttcag acaggatcag aagaacttag
     2881 atcattatat aatacagtag caaccctcta ttgtgtgcat caaaggatag agataaaaga
     2941 caccaaggaa gctttagaca agatagagga agagcaaaac aaaagtaaga ccaccgcaca
     3001 gcaagcggcc gctgatcttc agacctggag gaggagatat gagggacaat tggagaagtg
     3061 aattatataa atataaagta gtaaaaattg aaccattagg agtagcaccc accaaggcaa
     3121 agagaagagt ggtgcagaga gaaaaaagag cagtgggaat aggagctttg ttccttgggt
     3181 tcttgggagc agcaggaagc actatgggcg cagcgtcaat gacgctgacg gtacaggcca
     3241 gacaattatt gtctggtata gtgcagcagc agaacaattt gctgagggct attgaggcgc
     3301 aacagcatct gttgcaactc acagtctggg gcatcaagca gctccaggca agaatcctgg
     3361 ctgtggaaag atacctaaag gatcaacagc tcctggggat ttggggttgc tctggaaaac
     3421 tcatttgcac cactgctgtg ccttggaatg ctagttggag taataaatct ctggaacaga
     3481 tttggaatca cacgacctgg atggagtggg acagagaaat taacaattac acaagcttaa
     3541 tacactcctt aattgaagaa tcgcaaaacc agcaagaaaa gaatgaacaa gaattattgg
     3601 aattagataa atgggcaagt ttgtggaatt ggtttaacat aacaaattgg ctgtggtata
     3661 taaaattatt cataatgata gtaggaggct tggtaggttt aagaatagtt tttgctgtac
     3721 tttctatagt gaatagagtt aggcagggat attcaccatt atcgtttcag acccacctcc
     3781 caaccccgag gggacccgac aggcccgaag gaatagaaga agaaggtgga gagagagaca
     3841 gagacagatc cattcgatta gtgaacggat ctcgacggta tcggttaact tttaaaagaa
     3901 aaggggggat tggggggtac agtgcagggg aaagaatagt agacataata gcaacagaca
     3961 tacaaactaa agaattacaa aaacaaatta caaaattcaa aattttatcg ataagcttgg
     4021 gagttcaggg acagcagaga tccagtttat cggatgttta taaattgcga tgaggccctt
     4081 tcgtcttcag cagagatcca gtttatcgat attaaggctt atgcgatgag gccctttcgt
     4141 cttcactcga gtttactccc tatcagtgat agagaacgta tgaagagttt actccctatc
     4201 agtgatagag aacgtatgca gactttactc cctatcagtg atagagaacg tataaggagt
     4261 ttactcccta tcagtgatag agaacgtatg accagtttac tccctatcag tgatagagaa
     4321 cgtatctaca gtttactccc tatcagtgat agagaacgta tatccagttt actccctatc
     4381 agtgatagag aacgtatgtc gaggtaggcg tgtacggtgg gcgcctataa aagcagagct
     4441 cgtttagtga accgtcagat cgcctggagc aattccacaa cacttttgtc ttatacttgg
     4501 gtttgtctgg tcaaccaccg cggactcagt ggtgtacggt acaaaccgcc accatggtgt
     4561 ctaagggcga agagctgatt aaggagaaca tgcacatgaa gctgtacatg gagggcaccg
     4621 tggacaacca tcacttcaag tgcacatccg agggcgaagg caagccctac gagggcaccc
     4681 agaccatgag aatcaaggtg gtcgagggcg gccctctccc cttcgccttc gacatcctgg
     4741 ctactagctt cctctacggc agcaagacct tcatcaacca cacccagggc atccccgact
     4801 tcttcaagca gtccttccct gagggcttca catgggagag agtcaccaca tacgaagacg
     4861 ggggcgtgct gaccgctacc caggacacca gcctccagga cggctgcctc atctacaacg
     4921 tcaagatcag aggggtgaac ttcacatcca acggccctgt gatgcagaag aaaacactcg
     4981 gctgggaggc cttcaccgag acgctgtacc ccgctgacgg cggcctggaa ggcagaaacg
     5041 acatggccct gaagctcgtg ggcgggagcc atctgatcgc aaacgccaag accacatata
     5101 gatccaagaa acccgctaag aacctcaaga tgcctggcgt ctactatgtg gactacagac
     5161 tggaaagaat caaggaggcc aacaacgaga cctacgtcga gcagcacgag gtggcagtgg
     5221 ccagatactg cgacctccct agcaaactgg ggcacaagct taatgggagc ggagctacta
     5281 acttcagcct gctgaagcag gctggagacg tggaggagaa ccctggacct tccgccaccc
     5341 tcgagggagt gcaggtggag actatctccc caggagacgg gcgcaccttc cccaagcgcg
     5401 gccagacctg cgtggtgcac tacaccggga tgcttgaaga tggaaagaaa gttgattcct
     5461 cccgggacag aaacaagccc tttaagttta tgctaggcaa gcaggaggtg atccgaggct
     5521 gggaagaagg ggttgcccag atgagtgtgg gtcagagagc caaactgact atatctccag
     5581 attatgccta tggtgccact gggcacccag gcatcatccc accacatgcc actctcgtct
     5641 tcgatgtgga gcttctaaaa ctggaatctg gcggtggatc cggagtcgac ggatttggtg
     5701 atgtcggtgc tcttgagagt ttgaggggaa atgcagattt ggcttacatc ctgagcatgg
     5761 agccctgtgg ccactgcctc attatcaaca atgtgaactt ctgccgtgag tccgggctcc
     5821 gcacccgcac tggctccaac atcgactgtg agaagttgcg gcgtcgcttc tcctcgctgc
     5881 atttcatggt ggaggtgaag ggcgacctga ctgccaagaa aatggtgctg gctttgctgg
     5941 agctggcgcg gcaggaccac ggtgctctgg actgctgcgt ggtggtcatt ctctctcacg
     6001 gctgtcaggc cagccacctg cagttcccag gggctgtcta cggcacagat ggatgccctg
     6061 tgtcggtcga gaagattgtg aacatcttca atgggaccag ctgccccagc ctgggaggga
     6121 agcccaagct ctttttcatc caggcctgtg gtggggagca gaaagaccat gggtttgagg
     6181 tggcctccac ttcccctgaa gacgagtccc ctggcagtaa ccccgagcca gatgccaccc
     6241 cgttccagga aggtttgagg accttcgacc agctggacgc catatctagt ttgcccacac
     6301 ccagtgacat ctttgtgtcc tactctactt tcccaggttt tgtttcctgg agggacccca
     6361 agagtggctc ctggtacgtt gagaccctgg acgacatctt tgagcagtgg gctcactctg
     6421 aagacctgca gtccctcctg cttagggtcg ctaatgctgt ttcggtgaaa gggatttata
     6481 aacagatgcc tggttgcttt aatttcctcc ggaaaaaact tttctttaaa acatcagtcg
     6541 actatccgta cgacgtacca gactacgcac tcgactctgg cagcggagag ggcagaggaa
     6601 gtcttctaac atgcggtgac gtggaggaga atcccggccc tgccaagcct ttgtctcaag
     6661 aagaatccac cctcattgaa agagcaacgg ctacaatcaa cagcatcccc atctctgaag
     6721 actacagcgt cgccagcgca gctctctcta gcgacggccg catcttcact ggtgtcaatg
     6781 tatatcattt tactggggga ccttgtgcag aactcgtggt gctgggcact gctgctgctg
     6841 cggcagctgg caacctgact tgtatcgtcg cgatcggaaa tgagaacagg ggcatcttga
     6901 gcccctgcgg acggtgccga caggtgcttc tcgatctgca tcctgggatc aaagccatag
     6961 tgaaggacag tgatggacag ccgacggcag ttgggattcg tgaattgctg ccctctggtt
     7021 atgtgtggga gggctagtaa acgcgtgaat tcactcctca ggtgcaggct gcctatcaga
     7081 aggtggtggc tggtgtggcc aatgccctgg ctcacaaata ccactgagat ctttttccct
     7141 ctgccaaaaa ttatggggac atcatgaagc cccttgagca tctgacttct ggctaataaa
     7201 ggaaatttat tttcattgca atagtgtgtt ggaatttttt gtgtctctca ctcggaagga
     7261 catatgggag ggcaaatcat ttaaaacatc agaatgagta tttggtttag agtttggcaa
     7321 catatgccca tatgctggct gccatgaaca aaggttggct ataaagaggt catcagtata
     7381 tgaaacagcc ccctgctgtc cattccttat tccatagaaa agccttgact tgaggttaga
     7441 ttttttttat attttgtttt gtgttatttt tttctttaac atccctaaaa ttttccttac
     7501 atgttttact agccagattt ttcctcctct cctgactact cccagtcata gctgtccctc
     7561 ttctcttatg gagatctaaa ccgcgttaca taacttacgg taaatggccc gcctggctga
     7621 ccgcccaacg acccccgccc attgacgtca ataatgacgt atgttcccat agtaacgcca
     7681 atagggactt tccattgacg tcaatgggtg gagtatttac ggtaaactgc ccacttggca
     7741 gtacatcaag tgtatcatat gccaagtacg ccccctattg acgtcaatga cggtaaatgg
     7801 cccgcctggc attatgccca gtacatgacc ttatgggact ttcctacttg gcagtacatc
     7861 tacgtattag tcatcgctat taccatggtg atgcggtttt ggcagtacat caatgggcgt
     7921 ggatagcggt ttgactcacg gggatttcca agtctccacc ccattgacgt caatgggagt
     7981 ttgttttggc accaaaatca acgggacttt ccaaaatgtc gtaacaactc cgccccattg
     8041 acgcaaatgg gcggtaggcg tgtacggtgg gaggtctata taagcagagc tcgtttagtg
     8101 aaccgtcaga tcgcctggag acgccatcca cgctgttttg acctccatag aagacaccga
     8161 ctctagtcca gtgtggtgga attctgcaga tatcaacaag tttgtacaaa aaagttggca
     8221 ccatgtctag actggacaag agcaaagtca taaacggagc tctggaatta ctcaatggtg
     8281 tcggtatcga aggcctgacg acaaggaaac tcgctcaaaa gctgggagtt gagcagccta
     8341 ccctgtactg gcacgtgaag aacaagcggg ccctgctcga tgccctgcca atcgagatgc
     8401 tggacaggca tcatacccac ttctgccccc tggaaggcga gtcatggcaa gactttctgc
     8461 ggaacaacgc caagtcatac cgctgtgctc tcctctcaca tcgcgacggg gctaaagtgc
     8521 atctcggcac ccgcccaaca gagaaacagt acgaaaccct ggaaaatcag ctcgcgttcc
     8581 tgtgtcagca aggcttctcc ctggagaacg cactgtacgc tctgtccgcc gtgggccact
     8641 ttacactggg ctgcgtattg gaggaacagg agcatcaagt agcaaaagag gaaagagaga
     8701 cacctaccac cgattctatg cccccacttc tgagacaagc aattgagctg ttcgaccggc
     8761 agggagccga acctgccttc cttttcggcc tggaactaat catatgtggc ctggagaaac
     8821 agctaaagtg cgaaagcggc gggccgaccg acgcccttga cgattttgac ttagacatgc
     8881 tcccagccga tgcccttgac gattttgacc ttgacatgct ccccgggtaa gcacaattcg
     8941 agctcggtac ctttaagacc aatgacttac aaggcagctg tagatcttag ccacttttta
     9001 aaagaaaagg ggggactgga agggctagct cactcccaac gaagacaaga tctgcttttt
     9061 gcttgtactg ggtctctctg gttagaccag atctgagcct gggagctctc tggctaacta
     9121 gggaacccac tgcttaagcc tcaataaagc ttgccttgag tgcttcaagt agtgtgtgcc
     9181 cgtctgttgt gtgactctgg taactagaga tccctcagac ccttttagtc agtgtggaaa
     9241 atctctagca gtagtagttc atgtcatctt attattcagt atttataact tgcaaagaaa
     9301 tgaatatcag agagtgagag gaacttgttt attgcagctt ataatggtta caaataaagc
     9361 aatagcatca caaatttcac aaataaagca tttttttcac tgcattctag ttgtggtttg
     9421 tccaaactca tcaatgtatc ttatcatgtc tggctctagc tatcccgccc ctaactccgc
     9481 ccatcccgcc cctaactccg cccagttccg cccattctcc gccccatggc tgactaattt
     9541 tttttattta tgcagaggcc gaggccgcct cggcctctga gctattccag aagtagtgag
     9601 gaggcttttt tggaggccta gggacgtacc caattcgccc tatagtgagt cgtattacgc
     9661 gcgctcactg gccgtcgttt tacaacgtcg tgactgggaa aaccctggcg ttacccaact
     9721 taatcgcctt gcagcacatc cccctttcgc cagctggcgt aatagcgaag aggcccgcac
     9781 cgatcgccct tcccaacagt tgcgcagcct gaatggcgaa tgggacgcgc cctgtagcgg
     9841 cgcattaagc gcggcgggtg tggtggttac gcgcagcgtg accgctacac ttgccagcgc
     9901 cctagcgccc gctcctttcg ctttcttccc ttcctttctc gccacgttcg ccggctttcc
     9961 ccgtcaagct ctaaatcggg ggctcccttt agggttccga tttagtgctt tacggcacct
    10021 cgaccccaaa aaacttgatt agggtgatgg ttcacgtagt gggccatcgc cctgatagac
    10081 ggtttttcgc cctttgacgt tggagtccac gttctttaat agtggactct tgttccaaac
    10141 tggaacaaca ctcaacccta tctcggtcta ttcttttgat ttataaggga ttttgccgat
    10201 ttcggcctat tggttaaaaa atgagctgat ttaacaaaaa tttaacgcga attttaacaa
    10261 aatattaacg cttacaattt aggtggcact tttcggggaa atgtgcgcgg aacccctatt
    10321 tgtttatttt tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa
    10381 atgcttcaat aatattgaaa aaggaagagt
//
