LOCUS       sequence-394837-        8718 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Inducible promoter Pxyl for Haloferax volcanii.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8718)
  AUTHORS   Rados T, Andre K, Cerletti M, Bisson A
  TITLE     A sweet new set of inducible and constitutive promoters in Haloferax
            volcanii.
  JOURNAL   Front Microbiol. 2023 Aug 10;14:1204876. doi: 
            10.3389/fmicb.2023.1204876. eCollection 2023.
  PUBMED    37637112
REFERENCE   2  (bases 1 to 8718)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Front 
            Microbiol."; date: "2023-08-10"; volume: "14:1204876. doi"; pages: "
            10.3389/fmicb.2023.1204876. eCollection 2023"
COMMENT     Sequence Label: pAL750
FEATURES             Location/Qualifiers
     source          1..8718
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     3140..3157
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    3274..3295
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(3310..3315,3316..3333,3334..3340)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    3348..3364
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     3353..3375
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     3372..3388
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     3372..3388
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     3407..3427
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        3409..3427
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     3486..3502
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     3486..3502
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     primer_bind     complement(4307..4326)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(4308..4326)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(4336..4353)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(4336..4352)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4345..4367)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      4494..4852
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4581..4600)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4791..4812
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        6228..6332
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(6333..6401,6402..7193)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(6551..6570)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      7364..7952
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7853..7872
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 gcttgacgca tatctctctg agaaggtgga ttaataaact atagtcgtga ctagcgaact
       61 agttgaatca aacgacgccg ggagcacacg agtctgccag gacttcgctc ctatcggcgt
      121 cgaaggatgc ttacgcggac acggcgggcg ggtggcccgt tgtcctaact caaccaatgt
      181 ggttggggct tatgggatta tcggattttc acccggcgtt agtgttcgca caaaagcgac
      241 actaaatgta cgatacggac gatacgacgg cacacgaacc gactgagaag cgaagccttc
      301 gctttgcgac tgacctcgag gcggacgtct cgcttacaga ctggaccgcc cttcgggttc
      361 atcgagtaat cttcgacgag acagagagtc aatttgcgct tctcgaggca ctacaaacag
      421 cggcggacgg tgtcgacata ctcgagtttg aacaccgact gtcgagttgt gggtttgacg
      481 tcgatgggtc agtcgtgaaa cttgcacgac gagctgtcgc cggcgagaga cgggctctgt
      541 gggacttgat tcggtatgcg cgacggttgg gacatgatga gatgggacag ccacagagcg
      601 ccttagagga cgctctcgcg ttcactcgtc tattgggcta tagcaaccct caacagacga
      661 tagcaatcgg tctcaatgcg gctttcagag agacgcggcg cgaccaggcc gacgagatta
      721 tcgagtacgt tctaacgctg tctgagagcg tcgacgtggt ccttggtggg tcagagatcg
      781 accgagtgta cttgcacaac tactacggcg atattctccc atcgagtgtt acgaatccgt
      841 gcaacgacac ccgtaatgat tactccatta cgacgctcgt cgacgtggtt gggacggacg
      901 gacttgctgt cgagatactt cggacggtag gtgagactga aagcggacac atggcctaca
      961 gcgccctaca atcggcgttc agtcagtacg ataggaccac ggtctcgcgg cgattgaacg
     1021 agaagctaat cgagtatggt ctttgtgaga agtacggcgc tcgcggaaac ctacgaatcg
     1081 agattctcga caaaggagcg ttgtacctcg aagaattagc caaagagaaa gagcgacaaa
     1141 aggcccttca ggactacgtt acgaacaccc caaaaccaaa ccacaatacc cgtgtaatca
     1201 cggaccgccc tcggggaggg agggaggacg accaccgacc gaccgccgaa ggtgaggcgt
     1261 cgacgacgcc ggacggacgg gttcgcttac ctcggtatca cggtgtggac tatctttcgt
     1321 acgcagagac ggtctcagcg gcgaagacac cgccgaagaa aggcgttagt gtgacaaact
     1381 accctgtcga acccaaatct gaccggggag aagccgggtg gggctacctt gaggacgaga
     1441 atacgctcgt cgtctctgca gagtacgact ctgccatgtc tcatgtggtg atgattgcac
     1501 gagcgttact cgatcctaag acgttcgacc aggtccttac cgaggaccgt ctcgcggaac
     1561 ttgatggact aatcgaagat gggacgtacg ttcgtgggtc tcgaaacctc gggtggttgg
     1621 ctgactctgt cgactcggcg ggtgagtacg tcgacgtcct cgaggacgcg agagacgagc
     1681 tactcgacat gacgcggtca cttgcacatg aggactacga gtgtgagacg tccgagtacc
     1741 tgagtcgaat cacgaaaacg gcgatgggtc tcgcgggaac cgcgtttcac gtcctcgatt
     1801 tactcgatat tgacgtcgtt tgggaggctc gcttacccga ctacaaccga cacccggagc
     1861 gatacgggga ggacaatgcc gagttgttag caacgacgct tgcgaagaac gcgccgattg
     1921 cggcgacgta cgggaaccac gtagtccggc gactgttgtt tgaagaccga gacgagaagc
     1981 gacgtcagtc gttcgaccct gtcgtcgacg cgagcaatcc gtatgcgaac ctgattgcgt
     2041 cgatctcggt ggttggtgac ttcgggaatc gggcggacca cgtcgccggc gtaatagaag
     2101 accgactgtc gaaccctggg gagattcacg aggacgcgcc ggagattgcg ctcaaagtcc
     2161 ctgtggtcgt cgagcatggt ccgagtacgg ttgcacgagt aacacgggca atgtgtcggg
     2221 cgaaaggtct cgacgcgacg agagacgcga tacggctatt ctctgggttt gcacggacac
     2281 cgtacgacgt agcccacgca atcgggcgag ggttgtctca agaagcgaca ccgagagaga
     2341 ttcggagtag cgaagtacgg ctttcactcg cgagtctccc gtcgaagcgt ctcttagagg
     2401 acgcaacccc cacggttcgg gcgatgatta gtacgttgct cgcgacgaat ctgagcctta
     2461 gcaagacaga gttagccgag aaagcgggaa tctcgacgca gtcggttcgg aaccacttac
     2521 cgacacttgt agcaatgggg ttagtcgacg agaccgaggg tcagtgccga ctgaaccttt
     2581 cgttcacaga cgagcaagaa cgccacaagc ggattcttcc atcgttcatg ctgaccgagt
     2641 cgttcactcg agacgtcctc tttgagacgc tagaagcgct tgatgagttg accgaggagg
     2701 taatcgagat atgggtacaa tgcggaccta acgggctacc ggacatcgac cgtctccata
     2761 cgatgtacga ctgggtagac tgggtacgtc ccttactgtc gagtctaacc gaagaacgag
     2821 acgagacggt tcagacgctg tctattgggc cggagattgc acagcaaccc ctcacggagg
     2881 caggttcgtg attgaactaa cctttggtaa tatgttcgtg atatgcgttg tattcgggta
     2941 tctcgtaatc tcgtggtatc tctcgagaca gtacgttcat gattagagta gggtcgacga
     3001 actctgaacc tatgaatcgc ggtcggacaa caacccccca tgcatgttct ttcctgcgtt
     3061 atcccctgat tctgtggata accgtattac cgcctttgag tgagctgata ccgctcgccg
     3121 cagccgaacg accgagcgca gcgagtcagt gagcgaggaa gcggaagagc gcccaatacg
     3181 caaaccgcct ctccccgcgc gttggccgat tcattaatgc agctggcacg acaggtttcc
     3241 cgactggaaa gcgggcagtg agcgcaacgc aattaatgtg agttagctca ctcattaggc
     3301 accccaggct ttacacttta tgcttccggc tcgtatgttg tgtggaattg tgagcggata
     3361 acaatttcac acaggaaaca gctatgacca tgattacgcc aagcgcgcaa ttaaccctca
     3421 ctaaagggaa caaaagctgg agctccaccg cgccgaaaaa tgcgatggtc cagaggtgcg
     3481 gccgctctag aactagtgga tcctcatatg cagtatcctc attaccagcg attgtaataa
     3541 aagtacgtcc cggtatgggc ggcgcggggc aatgcagaaa accccgagac gtcggcgggt
     3601 tcggtctcga actcccggcc gggtgtagtt cgctcgtccg ttgacgacga gcgccgacga
     3661 gcgcgagatg acaggcagaa gaggcgacac gagcccgacg cgtgtgacgt agagagacac
     3721 acgaattccc agtaacagaa cacgccgagt cggcgggtcg aatcggcaac gcggcgacga
     3781 gacggccgtg tcggagccca cgagcgcgga ctgacgaccc acaccggtgt cgtcgaaaac
     3841 gaccaccgag agagacaagt cgattattac ggctcgtctc ggccgataat caggaacgag
     3901 tgggccgaga gttcgccaga cactgccgtt ttctatcaca gaatgcaatc acagcacgca
     3961 cgaacgagag agtccgggaa cagcccacgc gacgtgcggt gcgccggttt tagattacac
     4021 acatacgctt gtaattcggg tggccgacgc cgagaagggg ggcggggttc ccgagagtcc
     4081 ggagcaagag cgaacgcggg ggacgagtca ctcggcctcg cccttcgttc cctattacag
     4141 aacgattgtc cagcagtctc cggcgtctgc cggcggcaaa cctgtcggaa gcgacatcga
     4201 aactcccgtc gggaggggct agcgcgcgct cacgagagcg cgtcgatggc gaaacgggag
     4261 acgagagacg ggagacggga gacgaaagac gggggtaccc aattcgccct atagtgagtc
     4321 gtattacgcg cgctcactgg ccgtcgtttt acaacgtcgt gactgggaaa accctggcgt
     4381 tacccaactt aatcgccttg cagcacatcc ccctttcgcc agctggcgta atagcgaaga
     4441 ggcccgcacc gatcgccctt cccaacagtt gcgcagcctg aatggcgaat gggacgcgcc
     4501 ctgtagcggc gcattaagcg cggcgggtgt ggtggttacg cgcagcgtga ccgctacact
     4561 tgccagcgcc ctagcgcccg ctcctttcgc tttcttccct tcctttctcg ccacgttcgc
     4621 cggctttccc cgtcaagctc taaatcgggg gctcccttta gggttccgat ttagtgcttt
     4681 acggcacctc gaccccaaaa aacttgatta gggtgatggt tcacgtagtg ggccatcgcc
     4741 ctgatagacg gtttttcgcc ctttgacgtt ggagtccacg ttctttaata gtggactctt
     4801 gttccaaact ggaacaacac tcaaccctat ctcggtctat tcttttgatt tagatcccgt
     4861 ggataaaacc cctcgttgac gccgcgggca gcgtcgtgat ggcggtcaca cccgggcttt
     4921 cgtggcagta cgctggcccg aacagcaact actatgcgtt cggaagccgc actctgcagc
     4981 catggcgaac gcagcactca tcgaggcgct ccgagccgcc gacgcggtcc agttcggcga
     5041 gttcgagctc tcccacggcg gcacgtcgag ctactacgtg gacaagtacc tcttcgagac
     5101 cgacccccgg tgtctgaagc tcatcgccga agcgttcgca gagcgcgtcg cggacgacga
     5161 caaactggcc ggcgtcgcgc tcggcgcggt cccgctcgtg gccgccaccg cggtcgagac
     5221 gaacaagccg tacgtcatcg cccgcaagaa ggcaaaggag tacgggacgg caaagcgcat
     5281 cgagggcgcg ctcgacgagg gcgaggaagt cgtcgtcctc gaagacatcg cgacgaccgg
     5341 ccagagcgcc gtcgacgccg tggaggcgct ccgcgaggcc ggcgcagtcg tcgaccgcgt
     5401 cgtcgtcgtc gtggaccgac aggagggcgc ggcggagctg ctggccgacc acgacatcgc
     5461 actggagtcg cttctcaccg ccgaggacct gctggccgac gccgacggct aatctagatg
     5521 agcggcgagg agctttctgg gtccgaaacc gagggcgacg ccgcggtccg gttcgtcctc
     5581 gtcgccgccg tcgccgacaa ccgcgtcatc ggccgcgacg gcgacatgcc gtggcacctc
     5641 cccgaggacc tgaaacagtt caagcggacg acgaccggcc acccggtcgt gatgggtcgc
     5701 aagacctacg agagcatcgc ccgccaactc ggcggcccgc ttccggagcg tcacagcgtc
     5761 gtgctgacga cccgcgacct cgacctcccc gagggcgctg aagcggtcga gtcggtcgag
     5821 tcggcggtcg ccgccgccga ggacgcggcc gacgagatgg gcgtcgagac cgtctacgtc
     5881 gtcggcggcg cgaccgtcta cgagcagttc ctcggccgcg ccgcggggct ggtgctcacc
     5941 gaactcgacg cggcacacga aggcgacacg cggttccccg agtgggaccg cgggaagtgg
     6001 gtcgagaccg accgcgacga ccgcgacggc ttttcgttcg tcacgtacga gcgcaaacag
     6061 cccgccgcgg ctgaccgcgg cgcggaggaa tccgatgagt aagcttaagg gattttgccg
     6121 atttcggcct attggttaaa aaatgagctg atttaacaaa aatttaacgc gaattttaac
     6181 aaaatattaa cgcttacaat ttaggtggca cttttcgggg aaatgtgcgc ggaaccccta
     6241 tttgtttatt tttctaaata cattcaaata tgtatccgct catgagacaa taaccctgat
     6301 aaatgcttca ataatattga aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc
     6361 ttattccctt ttttgcggca ttttgccttc ctgtttttgc tcacccagaa acgctggtga
     6421 aagtaaaaga tgctgaagat cagttgggtg cacgagtggg ttacatcgaa ctggatctca
     6481 acagcggtaa gatccttgag agttttcgcc ccgaagaacg ttttccaatg atgagcactt
     6541 ttaaagttct gctatgtggc gcggtattat cccgtattga cgccgggcaa gagcaactcg
     6601 gtcgccgcat acactattct cagaatgact tggttgagta ctcaccagtc acagaaaagc
     6661 atcttacgga tggcatgaca gtaagagaat tatgcagtgc tgccataacc atgagtgata
     6721 acactgcggc caacttactt ctgacaacga tcggaggacc gaaggagcta accgcttttt
     6781 tgcacaacat gggggatcat gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag
     6841 ccataccaaa cgacgagcgt gacaccacga tgcctgtagc aatggcaaca acgttgcgca
     6901 aactattaac tggcgaacta cttactctag cttcccggca acaattaata gactggatgg
     6961 aggcggataa agttgcagga ccacttctgc gctcggccct tccggctggc tggtttattg
     7021 ctgataaatc tggagccggt gagcgtgggt ctcgcggtat cattgcagca ctggggccag
     7081 atggtaagcc ctcccgtatc gtagttatct acacgacggg gagtcaggca actatggatg
     7141 aacgaaatag acagatcgct gagataggtg cctcactgat taagcattgg taactgtcag
     7201 accaagttta ctcatatata ctttagattg atttaaaact tcatttttaa tttaaaagga
     7261 tctaggtgaa gatccttttt gataatctca tgaccaaaat cccttaacgt gagttttcgt
     7321 tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc
     7381 tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc
     7441 cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac
     7501 caaatactgt tcttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac
     7561 cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt
     7621 cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct
     7681 gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat
     7741 acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag gcggacaggt
     7801 atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg
     7861 cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt
     7921 gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc tttttacggt
     7981 tcctggcctt ttgctggcct tttgctcaca tgagcttctt tgattcgagc catttgttcc
     8041 tcgccggtta ggtgggagag ttcgcccgat tcgagtagtt ggttcacctc ggggtaactg
     8101 agttcgtccg gctcgcgctc ccgtaactcg tcgagcggct cgccccgttt tacattaacc
     8161 tcgacctcga cctcctcgac cgaatacccg cgctggaggt atgcctgtct aacaccgctc
     8221 gcggcaatcc cctcgaaagc gtcctttgcc aaatcggacg gttcgacggt gtcggtgatc
     8281 ccgagatata ggagagcgaa cgaccacgac aggacgttta gcgtatgctc tttcccgtct
     8341 aaatcgccgt cctcgaaggc ttccgttcta aggtcctcgt cgagttccga aaccaggagc
     8401 ggcatatcat gtagggcttc atggagccgc tcgactatcg ctcgccggcg ttcgtatcct
     8461 gcctgtttgc tatactcctt cggtttttgg agataccggc ggtcggccgg tgacaaaatc
     8521 cctcttgccc tttcgtcaag actcatgctt taccaatcac atcttcatgt ccgtaatgct
     8581 ttatacgttg tgccctaaag cgggatatat gtctgccacg gacacgcgga ttccggtgtc
     8641 gaaggatgtg cggcgcgacc tccgggttct caaggcgcgg gaaggccgac ggtcatacga
     8701 tgagacaatt gcggtggt
//
