LOCUS       sequence-394838-        8180 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Contains 250 bp region of Pxyl promoter; insensitive to xylose..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8180)
  AUTHORS   Rados T, Andre K, Cerletti M, Bisson A
  TITLE     A sweet new set of inducible and constitutive promoters in Haloferax
            volcanii.
  JOURNAL   Front Microbiol. 2023 Aug 10;14:1204876. doi: 
            10.3389/fmicb.2023.1204876. eCollection 2023.
  PUBMED    37637112
REFERENCE   2  (bases 1 to 8180)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Front 
            Microbiol."; date: "2023-08-10"; volume: "14:1204876. doi"; pages: "
            10.3389/fmicb.2023.1204876. eCollection 2023"
COMMENT     Sequence Label: pAL250
FEATURES             Location/Qualifiers
     source          1..8180
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     448..467
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     4453..4470
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    4587..4608
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(4623..4628,4629..4646,4647..4653)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    4661..4677
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     4666..4688
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     4685..4701
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     4685..4701
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     4720..4740
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        4722..4740
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     4799..4815
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     4799..4815
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     primer_bind     complement(5082..5101)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(5083..5101)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(5111..5128)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(5111..5127)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5120..5142)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      5269..5627
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5356..5375)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     5566..5587
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        7003..7107
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(7108..7176,7177..7968)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(7326..7345)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      join(8139..8180,1..547)
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac caactctttt
       61 tccgaaggta actggcttca gcagagcgca gataccaaat actgttcttc tagtgtagcc
      121 gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg ctctgctaat
      181 cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgggt tggactcaag
      241 acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc
      301 cagcttggag cgaacgacct acaccgaact gagataccta cagcgtgagc tatgagaaag
      361 cgccacgctt cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac
      421 aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg
      481 gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct
      541 atggaaaaac gccagcaacg cggccttttt acggttcctg gccttttgct ggccttttgc
      601 tcacatgagc ttctttgatt cgagccattt gttcctcgcc ggttaggtgg gagagttcgc
      661 ccgattcgag tagttggttc acctcggggt aactgagttc gtccggctcg cgctcccgta
      721 actcgtcgag cggctcgccc cgttttacat taacctcgac ctcgacctcc tcgaccgaat
      781 acccgcgctg gaggtatgcc tgtctaacac cgctcgcggc aatcccctcg aaagcgtcct
      841 ttgccaaatc ggacggttcg acggtgtcgg tgatcccgag atataggaga gcgaacgacc
      901 acgacaggac gtttagcgta tgctctttcc cgtctaaatc gccgtcctcg aaggcttccg
      961 ttctaaggtc ctcgtcgagt tccgaaacca ggagcggcat atcatgtagg gcttcatgga
     1021 gccgctcgac tatcgctcgc cggcgttcgt atcctgcctg tttgctatac tccttcggtt
     1081 tttggagata ccggcggtcg gccggtgaca aaatccctct tgccctttcg tcaagactca
     1141 tgctttacca atcacatctt catgtccgta atgctttata cgttgtgccc taaagcggga
     1201 tatatgtctg ccacggacac gcggattccg gtgtcgaagg atgtgcggcg cgacctccgg
     1261 gttctcaagg cgcgggaagg ccgacggtca tacgatgaga caattgcggt ggtgcttgac
     1321 gcatatctct ctgagaaggt ggattaataa actatagtcg tgactagcga actagttgaa
     1381 tcaaacgacg ccgggagcac acgagtctgc caggacttcg ctcctatcgg cgtcgaagga
     1441 tgcttacgcg gacacggcgg gcgggtggcc cgttgtccta actcaaccaa tgtggttggg
     1501 gcttatggga ttatcggatt ttcacccggc gttagtgttc gcacaaaagc gacactaaat
     1561 gtacgatacg gacgatacga cggcacacga accgactgag aagcgaagcc ttcgctttgc
     1621 gactgacctc gaggcggacg tctcgcttac agactggacc gcccttcggg ttcatcgagt
     1681 aatcttcgac gagacagaga gtcaatttgc gcttctcgag gcactacaaa cagcggcgga
     1741 cggtgtcgac atactcgagt ttgaacaccg actgtcgagt tgtgggtttg acgtcgatgg
     1801 gtcagtcgtg aaacttgcac gacgagctgt cgccggcgag agacgggctc tgtgggactt
     1861 gattcggtat gcgcgacggt tgggacatga tgagatggga cagccacaga gcgccttaga
     1921 ggacgctctc gcgttcactc gtctattggg ctatagcaac cctcaacaga cgatagcaat
     1981 cggtctcaat gcggctttca gagagacgcg gcgcgaccag gccgacgaga ttatcgagta
     2041 cgttctaacg ctgtctgaga gcgtcgacgt ggtccttggt gggtcagaga tcgaccgagt
     2101 gtacttgcac aactactacg gcgatattct cccatcgagt gttacgaatc cgtgcaacga
     2161 cacccgtaat gattactcca ttacgacgct cgtcgacgtg gttgggacgg acggacttgc
     2221 tgtcgagata cttcggacgg taggtgagac tgaaagcgga cacatggcct acagcgccct
     2281 acaatcggcg ttcagtcagt acgataggac cacggtctcg cggcgattga acgagaagct
     2341 aatcgagtat ggtctttgtg agaagtacgg cgctcgcgga aacctacgaa tcgagattct
     2401 cgacaaagga gcgttgtacc tcgaagaatt agccaaagag aaagagcgac aaaaggccct
     2461 tcaggactac gttacgaaca ccccaaaacc aaaccacaat acccgtgtaa tcacggaccg
     2521 ccctcgggga gggagggagg acgaccaccg accgaccgcc gaaggtgagg cgtcgacgac
     2581 gccggacgga cgggttcgct tacctcggta tcacggtgtg gactatcttt cgtacgcaga
     2641 gacggtctca gcggcgaaga caccgccgaa gaaaggcgtt agtgtgacaa actaccctgt
     2701 cgaacccaaa tctgaccggg gagaagccgg gtggggctac cttgaggacg agaatacgct
     2761 cgtcgtctct gcagagtacg actctgccat gtctcatgtg gtgatgattg cacgagcgtt
     2821 actcgatcct aagacgttcg accaggtcct taccgaggac cgtctcgcgg aacttgatgg
     2881 actaatcgaa gatgggacgt acgttcgtgg gtctcgaaac ctcgggtggt tggctgactc
     2941 tgtcgactcg gcgggtgagt acgtcgacgt cctcgaggac gcgagagacg agctactcga
     3001 catgacgcgg tcacttgcac atgaggacta cgagtgtgag acgtccgagt acctgagtcg
     3061 aatcacgaaa acggcgatgg gtctcgcggg aaccgcgttt cacgtcctcg atttactcga
     3121 tattgacgtc gtttgggagg ctcgcttacc cgactacaac cgacacccgg agcgatacgg
     3181 ggaggacaat gccgagttgt tagcaacgac gcttgcgaag aacgcgccga ttgcggcgac
     3241 gtacgggaac cacgtagtcc ggcgactgtt gtttgaagac cgagacgaga agcgacgtca
     3301 gtcgttcgac cctgtcgtcg acgcgagcaa tccgtatgcg aacctgattg cgtcgatctc
     3361 ggtggttggt gacttcggga atcgggcgga ccacgtcgcc ggcgtaatag aagaccgact
     3421 gtcgaaccct ggggagattc acgaggacgc gccggagatt gcgctcaaag tccctgtggt
     3481 cgtcgagcat ggtccgagta cggttgcacg agtaacacgg gcaatgtgtc gggcgaaagg
     3541 tctcgacgcg acgagagacg cgatacggct attctctggg tttgcacgga caccgtacga
     3601 cgtagcccac gcaatcgggc gagggttgtc tcaagaagcg acaccgagag agattcggag
     3661 tagcgaagta cggctttcac tcgcgagtct cccgtcgaag cgtctcttag aggacgcaac
     3721 ccccacggtt cgggcgatga ttagtacgtt gctcgcgacg aatctgagcc ttagcaagac
     3781 agagttagcc gagaaagcgg gaatctcgac gcagtcggtt cggaaccact taccgacact
     3841 tgtagcaatg gggttagtcg acgagaccga gggtcagtgc cgactgaacc tttcgttcac
     3901 agacgagcaa gaacgccaca agcggattct tccatcgttc atgctgaccg agtcgttcac
     3961 tcgagacgtc ctctttgaga cgctagaagc gcttgatgag ttgaccgagg aggtaatcga
     4021 gatatgggta caatgcggac ctaacgggct accggacatc gaccgtctcc atacgatgta
     4081 cgactgggta gactgggtac gtcccttact gtcgagtcta accgaagaac gagacgagac
     4141 ggttcagacg ctgtctattg ggccggagat tgcacagcaa cccctcacgg aggcaggttc
     4201 gtgattgaac taacctttgg taatatgttc gtgatatgcg ttgtattcgg gtatctcgta
     4261 atctcgtggt atctctcgag acagtacgtt catgattaga gtagggtcga cgaactctga
     4321 acctatgaat cgcggtcgga caacaacccc ccatgcatgt tctttcctgc gttatcccct
     4381 gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg ccgcagccga
     4441 acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcccaat acgcaaaccg
     4501 cctctccccg cgcgttggcc gattcattaa tgcagctggc acgacaggtt tcccgactgg
     4561 aaagcgggca gtgagcgcaa cgcaattaat gtgagttagc tcactcatta ggcaccccag
     4621 gctttacact ttatgcttcc ggctcgtatg ttgtgtggaa ttgtgagcgg ataacaattt
     4681 cacacaggaa acagctatga ccatgattac gccaagcgcg caattaaccc tcactaaagg
     4741 gaacaaaagc tggagctcca ccgcgccgaa aaatgcgatg gtccagaggt gcggccgctc
     4801 tagaactagt ggatccatat gcagtatcct cattaccagc gattgtaata aaagtacgtc
     4861 ccggtatggg cggcgcgggg caatgcagaa aaccccgaga cgtcggcggg ttcggtctcg
     4921 aactcccggc cgggtgtagt tcgctcgtcc gttgacgacg agcgccgacg agcgcgagat
     4981 gacaggcaga agaggcgaca cgagcccgac gcgtgtgacg tagagagaca cacgaattcc
     5041 cagtaacaga acacgccgag tcggcgggtg tacccaattc gccctatagt gagtcgtatt
     5101 acgcgcgctc actggccgtc gttttacaac gtcgtgactg ggaaaaccct ggcgttaccc
     5161 aacttaatcg ccttgcagca catccccctt tcgccagctg gcgtaatagc gaagaggccc
     5221 gcaccgatcg cccttcccaa cagttgcgca gcctgaatgg cgaatgggac gcgccctgta
     5281 gcggcgcatt aagcgcggcg ggtgtggtgg ttacgcgcag cgtgaccgct acacttgcca
     5341 gcgccctagc gcccgctcct ttcgctttct tcccttcctt tctcgccacg ttcgccggct
     5401 ttccccgtca agctctaaat cgggggctcc ctttagggtt ccgatttagt gctttacggc
     5461 acctcgaccc caaaaaactt gattagggtg atggttcacg tagtgggcca tcgccctgat
     5521 agacggtttt tcgccctttg acgttggagt ccacgttctt taatagtgga ctcttgttcc
     5581 aaactggaac aacactcaac cctatctcgg tctattcttt tgatttagat cccgtggata
     5641 aaacccctcg ttgacgccgc gggcagcgtc gtgatggcgg tcacacccgg gctttcgtgg
     5701 cagtacgctg gcccgaacag caactactat gcgttcggaa gccgcactct gcagccatgg
     5761 cgaacgcagc actcatcgag gcgctccgag ccgccgacgc ggtccagttc ggcgagttcg
     5821 agctctccca cggcggcacg tcgagctact acgtggacaa gtacctcttc gagaccgacc
     5881 cccggtgtct gaagctcatc gccgaagcgt tcgcagagcg cgtcgcggac gacgacaaac
     5941 tggccggcgt cgcgctcggc gcggtcccgc tcgtggccgc caccgcggtc gagacgaaca
     6001 agccgtacgt catcgcccgc aagaaggcaa aggagtacgg gacggcaaag cgcatcgagg
     6061 gcgcgctcga cgagggcgag gaagtcgtcg tcctcgaaga catcgcgacg accggccaga
     6121 gcgccgtcga cgccgtggag gcgctccgcg aggccggcgc agtcgtcgac cgcgtcgtcg
     6181 tcgtcgtgga ccgacaggag ggcgcggcgg agctgctggc cgaccacgac atcgcactgg
     6241 agtcgcttct caccgccgag gacctgctgg ccgacgccga cggctaatct agatgagcgg
     6301 cgaggagctt tctgggtccg aaaccgaggg cgacgccgcg gtccggttcg tcctcgtcgc
     6361 cgccgtcgcc gacaaccgcg tcatcggccg cgacggcgac atgccgtggc acctccccga
     6421 ggacctgaaa cagttcaagc ggacgacgac cggccacccg gtcgtgatgg gtcgcaagac
     6481 ctacgagagc atcgcccgcc aactcggcgg cccgcttccg gagcgtcaca gcgtcgtgct
     6541 gacgacccgc gacctcgacc tccccgaggg cgctgaagcg gtcgagtcgg tcgagtcggc
     6601 ggtcgccgcc gccgaggacg cggccgacga gatgggcgtc gagaccgtct acgtcgtcgg
     6661 cggcgcgacc gtctacgagc agttcctcgg ccgcgccgcg gggctggtgc tcaccgaact
     6721 cgacgcggca cacgaaggcg acacgcggtt ccccgagtgg gaccgcggga agtgggtcga
     6781 gaccgaccgc gacgaccgcg acggcttttc gttcgtcacg tacgagcgca aacagcccgc
     6841 cgcggctgac cgcggcgcgg aggaatccga tgagtaagct taagggattt tgccgatttc
     6901 ggcctattgg ttaaaaaatg agctgattta acaaaaattt aacgcgaatt ttaacaaaat
     6961 attaacgctt acaatttagg tggcactttt cggggaaatg tgcgcggaac ccctatttgt
     7021 ttatttttct aaatacattc aaatatgtat ccgctcatga gacaataacc ctgataaatg
     7081 cttcaataat attgaaaaag gaagagtatg agtattcaac atttccgtgt cgcccttatt
     7141 cccttttttg cggcattttg ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta
     7201 aaagatgctg aagatcagtt gggtgcacga gtgggttaca tcgaactgga tctcaacagc
     7261 ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc caatgatgag cacttttaaa
     7321 gttctgctat gtggcgcggt attatcccgt attgacgccg ggcaagagca actcggtcgc
     7381 cgcatacact attctcagaa tgacttggtt gagtactcac cagtcacaga aaagcatctt
     7441 acggatggca tgacagtaag agaattatgc agtgctgcca taaccatgag tgataacact
     7501 gcggccaact tacttctgac aacgatcgga ggaccgaagg agctaaccgc ttttttgcac
     7561 aacatggggg atcatgtaac tcgccttgat cgttgggaac cggagctgaa tgaagccata
     7621 ccaaacgacg agcgtgacac cacgatgcct gtagcaatgg caacaacgtt gcgcaaacta
     7681 ttaactggcg aactacttac tctagcttcc cggcaacaat taatagactg gatggaggcg
     7741 gataaagttg caggaccact tctgcgctcg gcccttccgg ctggctggtt tattgctgat
     7801 aaatctggag ccggtgagcg tgggtctcgc ggtatcattg cagcactggg gccagatggt
     7861 aagccctccc gtatcgtagt tatctacacg acggggagtc aggcaactat ggatgaacga
     7921 aatagacaga tcgctgagat aggtgcctca ctgattaagc attggtaact gtcagaccaa
     7981 gtttactcat atatacttta gattgattta aaacttcatt tttaatttaa aaggatctag
     8041 gtgaagatcc tttttgataa tctcatgacc aaaatccctt aacgtgagtt ttcgttccac
     8101 tgagcgtcag accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc
     8161 gtaatctgct gcttgcaaac
//
