LOCUS       sequence-394839-        9105 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Plasmid self-excising recombination plasmid for Matreyek Bxb1(GT) 
            landing pad.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9105)
  AUTHORS   Roelle SM, Kamath ND, Matreyek KA
  TITLE     Mammalian Genomic Manipulation with Orthogonal Bxb1 DNA Recombinase 
            Sites for the Functional Characterization of Protein Variants.
  JOURNAL   ACS Synth Biol. 2023 Nov 3. doi: 10.1021/acssynbio.3c00355.
  PUBMED    37922210
REFERENCE   2  (bases 1 to 9105)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "ACS Synth 
            Biol. 2023 Nov 3. doi: 10.1021/acssynbio.3c00355."
COMMENT     Sequence Label: 
            G718A_BattB(GA)_TKterm_AttB(GT)_sGFP-PTEN-IRES-mCherry-P2A-HygroR_no
            Term_attP(GA)_IRES-UnaG-2A-Puro-Term
FEATURES             Location/Qualifiers
     source          1..9105
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    25..41
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(49..55,56..73,74..79))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    94..115
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(232..249)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(403..991)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(483..502)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(1162..1953,1954..2022))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     1785..1804
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(2023..2127)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(2153..2608)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(2290..2311)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     2502..2521
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     2735..2757
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     2749..2766
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     2750..2766
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     2773..2792
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        2773..2791
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    2845..2882
                     /label=Bxb1 attB
                     /bound_moiety="Mycobacteriophage Bxb1 integrase"
                     /note="Minimal attB site for the Mycopbacteriophage Bxb1 
                     integrase (Voutev and Mann, 2017)"
     primer_bind     complement(2928..2947)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    2972..3019
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     protein_bind    3217..3254
                     /label=Bxb1 attB
                     /bound_moiety="Mycobacteriophage Bxb1 integrase"
                     /note="Minimal attB site for the Mycopbacteriophage Bxb1 
                     integrase (Voutev and Mann, 2017)"
     CDS             3288..3335
                     /codon_start=1
                     /product="11th beta-strand of superfolder GFP"
                     /label=GFP11
                     /note="associates with GFP(1-10), consisting of the first 
                     10 beta-strands, to reconstitute fluorescent GFP (Cabantous
                     et al., 2005; Kamiyama et al., 2016)"
                     /translation="RDHMVLHEYVNAAGIT"
     misc_feature    join(4696..5270,5271..5273,5274..5282)
                     /label=IRES2
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(5428..5446)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     5696..5715
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     CDS             5979..6035
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             6048..7067
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="KPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRGYV
                     LRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLPET
                     ELPAVLQPVAEAMDAIAATDLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQTV
                     MDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMFGD
                     SQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDGNF
                     DDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAKE"
     protein_bind    7100..7147
                     /label=Bxb1 attP
                     /bound_moiety="Mycobacteriophage Bxb1 integrase"
                     /note="Minimal attP site for the Mycopbacteriophage Bxb1 
                     integrase (Voutev and Mann, 2017)"
     primer_bind     complement(7172..7188)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7172..7188)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     misc_feature    join(7213..7787,7788..7790,7791..7799)
                     /label=IRES2
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     CDS             8223..8279
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     primer_bind     8773..8793
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     polyA_signal    8984..9105
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(9021..9040)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     9075..9094
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
ORIGIN
        1 ggagatcccc tttagtgagg gaaattgtta tccgctcaca attccacaca acatacgagc
       61 cggaagcata aagtgtaaag cctggggtgc ctaatgagtg agctaactca cattaattgc
      121 gttgcgctca ctgcccgctt tccagtcggg aaacctgtcg tgccagctgc attaatgaat
      181 cggccaacgc gcggggagag gcggtttgcg tattgggcgc tcttccgctt cctcgctcac
      241 tgactcgctg cactcggtcg ttcggctgcg gcgagcggta tcagctcact caaaggcggt
      301 aatacggtta tccacagaat caggggataa cgcaggaaag aacatgtgag caaaaggcca
      361 gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg tttttccata ggctccgccc
      421 ccctgacgag catcacaaaa atcgacgctc aagtcagagg tggcgaaacc cgacaggact
      481 ataaagatac caggcgtttc cccctggaag ctccctcgtg cgctctcctg ttccgaccct
      541 gccgcttacc ggatacctgt ccgcctttct cccttcggga agcgtggcgc tttctcatag
      601 ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca
      661 cgaacccccc gttcagcccg accgctgcgc cttatccggt aactatcgtc ttgagtccaa
      721 cccggtaaga cacgacttat cgccactggc agcagccact ggtaacagga ttagcagagc
      781 gaggtatgta ggcggtgcta cagagttctt gaagtggtgg cctaactacg gctacactag
      841 aagaacagta tttggtatct gcgctctgct gaagccagtt accttcggaa aaagagttgg
      901 tagctcttga tccggcaaac aaaccaccgc tggtagcggt ggtttttttg tttgcaagca
      961 gcagattacg cgcagaaaaa aaggatctca agaagatcct ttgatctttt ctacggggtc
     1021 tgacgctcag tggaacgaaa actcacgtta agggattttg gtcatgagat tatcaaaaag
     1081 gatcttcacc tagatccttt taaattaaaa atgaagtttt aaatcaatct aaagtatata
     1141 tgagtaaact tggtctgaca gttaccaatg cttaatcagt gaggcaccta tctcagcgat
     1201 ctgtctattt cgttcatcca tagttgcctg actccccgtc gtgtagataa ctacgatacg
     1261 ggagggctta ccatctggcc ccagtgctgc aatgataccg cgagacccac gctcaccggc
     1321 tccagattta tcagcaataa accagccagc cggaagggcc gagcgcagaa gtggtcctgc
     1381 aactttatcc gcctccatcc agtctattaa ttgttgccgg gaagctagag taagtagttc
     1441 gccagttaat agtttgcgca acgttgttgc cattgctaca ggcatcgtgg tgtcacgctc
     1501 gtcgtttggt atggcttcat tcagctccgg ttcccaacga tcaaggcgag ttacatgatc
     1561 ccccatgttg tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg tcagaagtaa
     1621 gttggccgca gtgttatcac tcatggttat ggcagcactg cataattctc ttactgtcat
     1681 gccatccgta agatgctttt ctgtgactgg tgagtactca accaagtcat tctgagaata
     1741 gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaata cgggataata ccgcgccaca
     1801 tagcagaact ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa aactctcaag
     1861 gatcttaccg ctgttgagat ccagttcgat gtaacccact cgtgcaccca actgatcttc
     1921 agcatctttt actttcacca gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc
     1981 aaaaaaggga ataagggcga cacggaaatg ttgaatactc atactcttcc tttttcaata
     2041 ttattgaagc atttatcagg gttattgtct catgagcgga tacatatttg aatgtattta
     2101 gaaaaataaa caaatagggg ttccgcgcac atttccccga aaagtgccac ctaaattgta
     2161 agcgttaata ttttgttaaa attcgcgtta aatttttgtt aaatcagctc attttttaac
     2221 caataggccg aaatcggcaa aatcccttat aaatcaaaag aatagaccga gatagggttg
     2281 agtgttgttc cagtttggaa caagagtcca ctattaaaga acgtggactc caacgtcaaa
     2341 gggcgaaaaa ccgtctatca gggcgatggc ccactacgtg aaccatcacc ctaatcaagt
     2401 tttttggggt cgaggtgccg taaagcacta aatcggaacc ctaaagggag cccccgattt
     2461 agagcttgac ggggaaagcc ggcgaacgtg gcgagaaagg aagggaagaa agcgaaagga
     2521 gcgggcgcta gggcgctggc aagtgtagcg gtcacgctgc gcgtaaccac cacacccgcc
     2581 gcgcttaatg cgccgctaca gggcgcgtcc cattcgccat tcaggctgcg caactgttgg
     2641 gaagggcgat cggtgcgggc ctcttcgcta ttacgccagc tggcgaaagg gggatgtgct
     2701 gcaaggcgat taagttgggt aacgccaggg ttttcccagt cacgacgttg taaaacgacg
     2761 gccagtgaat tgtaatacga ctcactatag ggcgaattgg gtaccgggcc ccccctcgag
     2821 gtcgacgatg taggtcacgg caccggcttg tcgacgacgg cggactccgt cgtcaggatc
     2881 atccttccgg actcggagtt cttcgcccac cctaggggga ggctaactga aacacggaag
     2941 gagacaatac cggaaggaac ccgcgctatg acggcaataa aaagacagaa taaaacgcac
     3001 ggtgttgggt cgtttgttca taaacgcggg gttcggtccc agggctggca ctctgtcgat
     3061 accccaccga gaccccattg gggccaatac gcccgcgttt cttccttttc cccaccccac
     3121 cccccaagtt cgggtgaagg cccagggctc gcagccaacg tcggggcggc aggccctgcc
     3181 atagcctcag gttactcata tataagcgct cgagccggct tgtcgacgac ggcggtctcc
     3241 gtcgtcagga tcatccgcag attataccgc aactacacgc caccatgaga gatcacatgg
     3301 tgctgcacga atacgttaat gccgccggta taaccacgcg ttctagaggc agcggagcca
     3361 ccatgacagc catcatcaaa gagatcgtta gcagaaacaa aaggagatat caagaggatg
     3421 gattcgactt agacttgacc tatatttatc caaacattat tgctatggga tttcctgcag
     3481 aaagacttga aggcgtatac aggaacaata ttgatgatgt agtaaggttt ttggattcaa
     3541 agcataaaaa ccattacaag atatacaatc tttgtgctga aagacattat gacaccgcca
     3601 aatttaattg cagagttgca caatatcctt ttgaagacca taacccacca cagctagaac
     3661 ttatcaaacc cttttgtgaa gatcttgacc aatggctaag tgaagatgac aatcatgttg
     3721 cagcaattca ctgtaaagct ggaaagggac gaactggtgt aatgatatgt gcatatttat
     3781 tacatcgggg caaattttta aaggcacaag aggccctaga tttctatggg gaagtaagga
     3841 ccagagacaa aaagggagta actattccca gtcagaggcg ctatgtgtat tattatagct
     3901 acctgttaaa gaatcatctg gattatagac cagtggcact gttgtttcac aagatgatgt
     3961 ttgaaactat tccaatgttc agtggcggaa cttgcaatcc tcagtttgtg gtctgccagc
     4021 taaaggtgaa gatatattcc tccaattcag gacccacacg acgggaagac aagttcatgt
     4081 actttgagtt ccctcagccg ttacctgtgt gtggtgatat caaagtagag ttcttccaca
     4141 aacagaacaa gatgctaaaa aaggacaaaa tgtttcactt ttgggtaaat acattcttca
     4201 taccaggacc agaggaaacc tcagaaaaag tagaaaatgg aagtctatgt gatcaagaaa
     4261 tcgatagcat ttgcagtata gagcgtgcag ataatgacaa ggaatatcta gtacttactt
     4321 taacaaaaaa tgatcttgac aaagcaaata aagacaaagc caaccgatac ttttctccaa
     4381 attttaaggt gaagctgtac ttcacaaaaa cagtagagga gccgtcaaat ccagaggcta
     4441 gcagttcaac ttctgtaaca ccagatgtta gtgacaatga acctgatcat tatagatatt
     4501 ctgacaccac tgactctgat ccagagaatg aaccttttga tgaagatcag catacacaaa
     4561 ttacaaaagt ctagtaactt aagaattcac cggtctgacc tccttctccc tctcttcagg
     4621 tctgcagtcg acggtaccgc gggcccggga tccaccggat ctagataact gatcactgtg
     4681 gaatcagaga gactgcccct ctccctctcc cccccctaac gttactggcc gaagccgctt
     4741 ggaataaggc cggtgtgcgt ttgtctatat gttattttcc accatattgc cgtcttttgg
     4801 caatgtgagg gcccggaaac ctggccctgt cttcttgacg agcattccta ggggtctttc
     4861 ccctctcgcc aaaggaatgc aaggtctgtt gaatgtcgtg aaggaagcag ttcctctgga
     4921 agcttcttga agacaaacaa cgtctgtagc gaccctttgc aggcagcgga accccccacc
     4981 tggcgacagg tgtctctgcg gccaaaagcc acgtgtataa gatacacctg caaaggcggc
     5041 acaaccccag tgccacgttg tgagttggat agttgtggaa agagtcaaat ggctctcctc
     5101 aagcgtattc aacaaggggc tgaaggatgc ccagaaggta ccccattgta tgggatctga
     5161 tctggggcct cggtgcacat gctttacatg tgtttagtcg aggttaaaaa aacgtctagg
     5221 ccccccgaac cacggggacg tggttttcct ttgaaaaaca cgatgataat atggccacaa
     5281 ccatggtgag caagggcgag gaggataaca tggccatcat caaggagttc atgcgcttca
     5341 aggtgcacat ggagggctcc gtgaacggcc acgagttcga gatcgagggc gagggcgagg
     5401 gccgccccta cgagggcacc cagaccgcca agctgaaggt gaccaagggt ggccccctgc
     5461 ccttcgcctg ggacatcctg tcccctcagt tcatgtacgg ctccaaggcc tacgtgaagc
     5521 accccgccga catccccgac tacttgaagc tgtccttccc cgagggcttc aagtgggagc
     5581 gcgtgatgaa cttcgaggac ggcggcgtgg tgaccgtgac ccaggactcc tccctgcagg
     5641 acggcgagtt catctacaag gtgaagctgc gcggcaccaa cttcccctcc gacggccccg
     5701 taatgcagaa gaagaccatg ggctgggagg cctcctccga gcggatgtac cccgaggacg
     5761 gcgccctgaa gggcgagatc aagcagaggc tgaagctgaa ggacggcggc cactacgacg
     5821 ctgaggtcaa gaccacctac aaggccaaga agcccgtgca gctgcccggc gcctacaacg
     5881 tcaacatcaa gttggacatc acctcccaca acgaggacta caccatcgtg gaacagtacg
     5941 aacgcgccga gggccgccac tccaccggcg gcagcggagc tactaacttc agcctgctga
     6001 agcaggctgg agacgtggag gagaaccctg gacctgatag atccggaaag cctgaactca
     6061 ccgcgacgtc tgtcgagaag tttctgatcg aaaagttcga cagcgtctcc gacctgatgc
     6121 agctctcgga gggcgaagaa tctcgtgctt tcagcttcga tgtaggaggg cgtggatatg
     6181 tcctgcgggt aaatagctgc gccgatggtt tctacaaaga tcgttatgtt tatcggcact
     6241 ttgcatcggc cgcgctcccg attccggaag tgcttgacat tggggaattc agcgagagcc
     6301 tgacctattg catctcccgc cgtgcacagg gtgtcacgtt gcaagacctg cctgaaaccg
     6361 aactgcccgc tgttctgcag ccggtcgcgg aggccatgga tgcgatcgct gcgaccgatc
     6421 ttagccagac gagcgggttc ggcccattcg gaccgcaagg aatcggtcaa tacactacat
     6481 ggcgtgattt catatgcgcg attgctgatc cccatgtgta tcactggcaa actgtgatgg
     6541 acgacaccgt cagtgcgtcc gtcgcgcagg ctctcgatga gctgatgctt tgggccgagg
     6601 actgccccga agtccggcac ctcgtgcacg cggatttcgg ctccaacaat gtcctgacgg
     6661 acaatggccg cataacagcg gtcattgact ggagcgaggc gatgttcggg gattcccaat
     6721 acgaggtcgc caacatcttc ttctggaggc cgtggttggc ttgtatggag cagcagacgc
     6781 gctacttcga gcggaggcat ccggagcttg caggatcgcc gcggctccgg gcgtatatgc
     6841 tccgcattgg tcttgaccaa ctctatcaga gcttggttga cggcaatttc gatgatgcag
     6901 cttgggcgca gggtcgatgc gacgcaatcg tccgatccgg agccgggact gtcgggcgta
     6961 cacaaatcgc ccgcagaagc gcggccgtct ggaccgatgg ctgtgtagaa gtactcgccg
     7021 atagtggaaa ccgacgcccc agcactcgtc cgagggcaaa ggaataatag ggatccggag
     7081 atccccttta gtgagggttg gtttgtctgg tcaaccaccg cggactcagt ggtgtacggt
     7141 acaaaccaat ttcgagcttg gcgtaatcat ggtcatagct gtttcctgtg tactgtggaa
     7201 tcagagagac tgcccctctc cctctccccc ccctaacgtt actggccgaa gccgcttgga
     7261 ataaggccgg tgtgcgtttg tctatatgtt attttccacc atattgccgt cttttggcaa
     7321 tgtgagggcc cggaaacctg gccctgtctt cttgacgagc attcctaggg gtctttcccc
     7381 tctcgccaaa ggaatgcaag gtctgttgaa tgtcgtgaag gaagcagttc ctctggaagc
     7441 ttcttgaaga caaacaacgt ctgtagcgac cctttgcagg cagcggaacc ccccacctgg
     7501 cgacaggtgc ctctgcggcc aaaagccacg tgtataagat acacctgcaa aggcggcaca
     7561 accccagtgc cacgttgtga gttggatagt tgtggaaaga gtcaaatggc tctcctcaag
     7621 cgtattcaac aaggggctga aggatgccca gaaggtaccc cattgtatgg gatctgatct
     7681 ggggcctcgg tgcacatgct ttacatgtgt ttagtcgagg ttaaaaaaac gtctaggccc
     7741 cccgaaccac ggggacgtgg ttttcctttg aaaaacacga tgataatatg gccacaacca
     7801 tggttgaaaa atttgttggt acgtggaaaa tagcggattc tcataatttt ggcgaatatc
     7861 tgaaggctat cggggcgcct aaagagctga gtgatggagg cgatgccacg actacaactc
     7921 tgtatatttc acagaaggac ggtgataaaa tgaccgttaa gatcgagaat ggcccgccca
     7981 ccttcctgga tacacaggta aagtttaaac ttggtgaaga atttgatgag ttcccgagcg
     8041 acagacgcaa aggtgttaaa tccgtagtca acttggtcgg agaaaaactc gtatatgtcc
     8101 aaaaatggga tggcaaagag acaacctacg tgcgtgaaat taaagacggg aaactggttg
     8161 tgacgctgac aatgggcgac gtggtggcag tgcgctcgta ccggcgtgca accgaaagcg
     8221 gagctactaa cttcagcctg ctgaagcagg ctggagacgt ggaggagaac cctggaccta
     8281 ccgagtacaa gcccacggtg cgcctcgcca cccgcgacga cgtccccagg gccgtacgca
     8341 ccctcgccgc cgcgttcgcc gactaccccg ccacgcgcca caccgtcgat ccggaccgcc
     8401 acatcgagcg ggtcactgag ctgcaagaac tcttcctcac gcgcgtcggg ctcgacatcg
     8461 gcaaggtgtg ggtcgcggac gacggcgccg cggtggcggt ctggaccacg ccggagagcg
     8521 tcgaagcggg ggcggtgttc gccgagatcg gcccgcgcat ggccgagttg agcggttccc
     8581 ggctggccgc gcagcaacag atggaaggcc tcctggcgcc gcaccggccc aaggagcccg
     8641 cgtggttcct ggccaccgtc ggcgtctcgc ccgaccacca gggcaagggt ctgggcagcg
     8701 ccgtcgtgct ccccggagtg gaggcggccg agcgcgccgg ggtgcccgcc ttcctggaga
     8761 cctccgcgcc ccgcaacctc cccttctacg agcggctcgg cttcaccgtc accgccgacg
     8821 tcgaggtgcc cgaaggaccg cgcacctggt gcatgacccg caagtaatag ggatcctgat
     8881 cataatcagc cataccacat ttgtagaggt tttacttgct ttaaaaaacc tcccacacct
     8941 ccccctgaac ctgaaacata aaatgaatgc aattgttgtt gttaacttgt ttattgcagc
     9001 ttataatggt tacaaataaa gcaatagcat cacaaatttc acaaataaag catttttttc
     9061 actgcattct agttgtggtt tgtccaaact catcaatgta tctta
//
