LOCUS       sequence-394847-        5594 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Contains ACS2 promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5594)
  AUTHORS   Calabria J, Wang L, Rast-Somssich MI, Chen HW, Watt M, Persson S, 
            Andersen TG, Idnurm A, Somssich M
  TITLE     Resolving spatially distinct phytohormone response zones in 
            Arabidopsis thaliana roots colonized by Fusarium oxysporum.
  JOURNAL   J Exp Bot. 2025 Jan 17:erae516. doi: 10.1093/jxb/erae516.
  PUBMED    39823275
REFERENCE   2  (bases 1 to 5594)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Exp Bot. 
            2025 Jan 17:erae516. doi: 10.1093/jxb/erae516."
COMMENT     Sequence Label: pGG-PIP50
FEATURES             Location/Qualifiers
     source          1..5594
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(10..28)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        96..200
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(201..269,270..1061)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(419..438)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      1232..1820
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1721..1740
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     1974..1991
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    2108..2129
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(2144..2149,2150..2167,2168..2174)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2182..2198
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        2212..2230
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     2212..2229
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(5180..5199)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(5181..5199)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(5209..5231)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     complement(5425..5444)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     5544..5566
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
ORIGIN
        1 gacgaaaggg cctcgtgata cgcctatttt tataggttaa tgtcatgata ataatggttt
       61 cttagacgtc aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt
      121 tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat
      181 aatattgaaa aaggaagagt atgagtattc aacatttccg tgtcgccctt attccctttt
      241 ttgcggcatt ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg
      301 ctgaagatca gttgggtgca cgagtgggtt acatcgaact ggatctcaac agcggtaaga
      361 tccttgagag ttttcgcccc gaagaacgtt ttccaatgat gagcactttt aaagttctgc
      421 tatgtggcgc ggtattatcc cgtattgacg ccgggcaaga gcaactcggt cgccgcatac
      481 actattctca gaatgacttg gttgagtact caccagtcac agaaaagcat cttacggatg
      541 gcatgacagt aagagaatta tgcagtgctg ccataaccat gagtgataac actgcggcca
      601 acttacttct gacaacgatc ggaggaccga aggagctaac cgcttttttg cacaacatgg
      661 gggatcatgt aactcgcctt gatcgttggg aaccggagct gaatgaagcc ataccaaacg
      721 acgagcgtga caccacgatg cctgtagcaa tggcaacaac gttgcgcaaa ctattaactg
      781 gcgaactact tactctagct tcccggcaac aattaataga ctggatggag gcggataaag
      841 ttgcaggacc acttctgcgc tcggcccttc cggctggctg gtttattgct gataaatctg
      901 gagccggtga gcgtggctct cgaggtatca ttgcagcact ggggccagat ggtaagccct
      961 cccgtatcgt agttatctac acgacgggga gtcaggcaac tatggatgaa cgaaatagac
     1021 agatcgctga gataggtgcc tcactgatta agcattggta actgtcagac caagtttact
     1081 catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga
     1141 tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt
     1201 cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct
     1261 gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc
     1321 taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgttc
     1381 ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc
     1441 tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg
     1501 ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt
     1561 cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg
     1621 agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg
     1681 gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt
     1741 atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag
     1801 gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt
     1861 gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta
     1921 ttaccgcctt tgagtgagct gataccgctc gccgcagccg aacgaccgag cgcagcgagt
     1981 cagtgagcga ggaagcggaa gagcgcccaa tacgcaaacc gcctctcccc gcgcgttggc
     2041 cgattcatta atgcagctgg cacgacaggt ttcccgactg gaaagcgggc agtgagcgca
     2101 acgcaattaa tgtgagttag ctcactcatt aggcacccca ggctttacac tttatgcttc
     2161 cggctcgtat gttgtgtgga attgtgagcg gataacaatt tcacacgagc tatttaggtg
     2221 acactataga agtgaagctt ggtctcaacc tcggtcgatg taaatggatt aaattttata
     2281 atggtttgtg tacgtgtgag ggttttccac gagtgagagt gaaagcgtgg gttatacgtg
     2341 tcttctgctt gatttttgat tttttcgtta catgacaaca atattttatt tcaaaaatat
     2401 atcattcatg aaaatagata ttttgtttgc agttatagct aaactagata attaaatacc
     2461 tttttagata gtcacacgat aaatttaata ccaaaaaaat ggaaagtatt atacaaaact
     2521 aaaagaggga aaagtattcg cgtaaggaat aagatgcgca agggattaat ggaccgacca
     2581 tgtcccccca tatgcacgtc ggtggaatgt agtagtactc ccatgcattg cggtacacta
     2641 tatggacaat gcacttatga cccgtattgg tatgggtttc tgatggatat taaggtcaaa
     2701 cttaaatggg ccacaggcca taagcccatt caaatttgta actggactat gatcactgtg
     2761 aagtcgtgaa tcttcttctc ttaaaaacaa aactttgaca tgatcactgt gaagtcgtgc
     2821 actgtaggca tgtcacgtgt tgatggatga ttagtggaaa gggattggaa gaagcgtgtc
     2881 caattagtcg acagctgcgg aatggaaaaa ctgagtcagc atttatgtta agaagtcaaa
     2941 cctacgcctc gcatggtttg ttagttaatt tgacttattg accagtcaaa gcatgctttg
     3001 acattttagt gatacacaaa ttttataata taaatccgca aacactagat tatattcaca
     3061 cttctcttta attagtgttt ggttccacga ttttcaacgg ccgattgtac tttttggttg
     3121 caaaatttta caaaacggta ccttttgttt tgtacatttt aaaaatcaac ttattattta
     3181 ttggcatcta tagttaggga aaacgtttca attgaacatg tacgtgtgca taaatcaaaa
     3241 ttttatttaa aatatacaga ttcgcttaag gagtataata acactccacg aacgaaattg
     3301 ttatgtatat attacatata taatttgtaa tcagtgaatt aagacattca aataattgaa
     3361 attaattaaa cttttaccaa gaaaataaat ttgataaata ataattgaaa ttttggaatg
     3421 atatcaaaac tgccgaaagc acatatgtcg gtgttggtag ttggcgggcc aattagtata
     3481 cgaatacgaa ctccttgttc ggtgtttctt ttacaaatta ttttctttta ttcataatct
     3541 atttctaatt cattagatgt ttcttacctg catttcatga gaaacacatc caaaaaaaaa
     3601 aactatttac aaacaaaaag ttcttcttag acaaaataat atattcacaa agctacaatg
     3661 tttccaaact tgataagaga atattagttt aggactgtct ttaattattg tttacacaag
     3721 ggattcgtag gatcccaaaa aacaatgggt tcgttggttt tgtttgaaag tgtagatgta
     3781 acgactccaa atagccaaaa gttgatatct tcagtctata ttattgagat cttatgtccc
     3841 ccctgtaatt aatttttcca gcaatgcgtg cttaaagaat attgtttgag actttagtta
     3901 ttgtaatacg acattagtat aagtagagcc aaaatcagat tttaatatct ttagtcttaa
     3961 gacatgaaac aagattaaga aaataccttg ttttcaaaga aaacggttat aaaaaggagg
     4021 atttgagttt ttgacattca gacgataaaa attatgaact aggtctagtc acgtggtcga
     4081 cgcgtgagag tttccggcgt gaactgcaag taaaatcacg tagagcatgt gattgacttg
     4141 accaaagagt ccaaacccac caactacaaa aaaaaatcaa gatataaata actaactctc
     4201 actagtcact aatataattt ttcattacaa ttcatatatg atctactaac aattgttgtg
     4261 gttatacaaa caaaaaattt atttttcgta agacggaaat tttgaaatca aattcctcaa
     4321 cactcaaatg aatattcagt agtagtttat caatgactag attagatatt tcttaacgcc
     4381 agtcaaattt tggaattatg tggaggacgt acgtttatac atgtgcagac tacaacatac
     4441 caaatgtttt attaaaccaa attacaatgt tgcaaattgg tctattcttt tgaataatct
     4501 gatacatttt atctcataac tttcttcctt tttatttgaa ttcaatcaaa taatattctc
     4561 cacatcccca accttctttt tttttttgca tgactaagta gtttaaggtc aacatttttc
     4621 ataagaagtt gcttagaaat agccttgggt tcaaataaaa tacacatgat tttcccgttt
     4681 ccaccaataa atcccaatgg attttaatac tgaaacggaa atcaatgcga aactattgga
     4741 gtaagaccaa tttattcatc ttaatctacc aaattcggat acgatatgtt taatacaagg
     4801 gagattgatg ctagcaaaac acaaccatct taattttttt ttttttttta attagaaatt
     4861 cccttcccaa atggtaattc aatcgtaaca aaagtacgtt ttgaaatatt gttttggatg
     4921 gagatttttt ccttggttcg cttgttactt ttcacttgtt tcatcaaatc ctaactcctt
     4981 ttattttgga ccccacatca actttatttg gtgtcctcaa ggtttctgtt tcaactccta
     5041 tataaaagca aataactcat acgttaatta gtacacacca caaaaacttg tcataagatc
     5101 aatatcgata cccccaaaaa aaaaaaaaac agctacaaag aagtgagaat tgacacagca
     5161 acatgagacc gaattctcgc cctatagtga gtcgtattac ctggccgtcg ttttacaacg
     5221 tcgtgactgg gaaaaccctg gcgttaccca acttaatcgc cttgcagcac atcccccttt
     5281 cgccagctgg cgtaatagcg aagaggcccg caccgatcgc ccttcccaac agttgcgcag
     5341 cctgaatggc gaatggcgcc tgatgcggta ttttctcctt acgcatctgt gcggtatttc
     5401 acaccgcata tggtgcactc tcagtacaat ctgctctgat gccgcatagt taagccagcc
     5461 ccgacacccg ccaacacccg ctgacgcgcc ctgacgggct tgtctgctcc cggcatccgc
     5521 ttacagacaa gctgtgaccg tctccgggag ctgcatgtgt cagaggtttt caccgtcatc
     5581 accgaaacgc gcga
//
