LOCUS       sequence-394849-        8887 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  TALE fused to N-terminal part of split mVenus.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8887)
  AUTHORS   Lungu C, Pinter S, Broche J, Rathert P, Jeltsch A
  TITLE     Modular fluorescence complementation sensors for live cell detection
            of epigenetic signals at endogenous genomic sites.
  JOURNAL   Nat Commun. 2017 Sep 21;8(1):649. doi: 10.1038/s41467-017-00457-z.
  PUBMED    28935858
REFERENCE   2  (bases 1 to 8887)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2017-09-21"; volume: "8(1):649. doi"; pages: " 
            10.1038/s41467-017-00457-z"
COMMENT     Sequence Label: pTH-PanCen TALE-mVenusN
FEATURES             Location/Qualifiers
     source          1..8887
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(44..63)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        235..614
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        615..818
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     769..789
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     protein_bind    820..838
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    841..859
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     869..893
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     regulatory      1005..1014
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             1014..1079
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     CDS             1086..1106
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             join(3645..3647,3648..4160)
                     /codon_start=1
                     /product="N-terminal fragment of mVenus for use in 
                     bimolecular fluorescence complementation (BiFC) (Kodama and
                     Hu, 2010)"
                     /label=VN173
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     LICTTGKLPVPWPTLVTTLGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYITADKQKNGIKA
                     NFKIRHNIE"
     primer_bind     complement(3687..3708)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(3948..3967)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     complement(4309..4326)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    4315..4539
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      4585..5013
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4672..4691)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4882..4903
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(5022..5042)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        5027..5356
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      5207..5342
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     5269..5288
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     CDS             5405..6430
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     CDS             5405..6430
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     polyA_signal    6560..6681
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6597..6616)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6651..6670
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(6730..6746)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6730..6746)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(6743..6765)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    6754..6770
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(6778..6784,6785..6802,6803..6808))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6823..6844
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(6961..6978)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7132..7720)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7212..7231)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7891..8682,8683..8751))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8514..8533
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8752..8856)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg
       61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
      121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattccatg aagaatctgc
      181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt
      241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata
      301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
      361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
      421 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt
      481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
      541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
      601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg
      661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc
      721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg
      781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ccctatcagt gatagagatc
      841 tccctatcag tgatagagat cgtcgacgag ctcgtttagt gaaccgtcag atcgcctgga
      901 gacgccatcc acgctgtttt gacctccata gaagacaccg ggaccgatcc agcctggcgc
      961 gccggttctg gatccggttc tcaattgggt tctaccggtt aggggccacc atggactata
     1021 aggaccacga cggagactac aaggatcatg atattgatta caaagacgat gacgataaga
     1081 tggccccaaa gaagaagcgg aaggtcggta tccacggagt cccagcagcc gtagatttga
     1141 gaactttggg atattcacag cagcagcagg aaaagatcaa gcccaaagtg aggtcgacag
     1201 tcgcgcagca tcacgaagcg ctggtgggtc atgggtttac acatgcccac atcgtagcct
     1261 tgtcgcagca ccctgcagcc cttggcacgg tcgccgtcaa gtaccaggac atgattgcgg
     1321 cgttgccgga agccacacat gaggcgatcg tcggtgtggg gaaacagtgg agcggagccc
     1381 gagcgcttga ggccctgttg acggtcgcgg gagagctgag agggcctccc cttcagctgg
     1441 acacgggcca gttgctgaag atcgcgaagc ggggaggagt cacggcggtc gaggcggtgc
     1501 acgcgtggcg caatgcgctc acgggagcac ccctcaacct gaccccagag caggtcgtgg
     1561 caattgcgag caacatcggg ggaaagcagg cactcgaaac cgtccagagg ttgctgcctg
     1621 tgctgtgcca agcgcacgga cttacgccag agcaggtcgt ggcaattgcg agcaaccacg
     1681 ggggaaagca ggcactcgaa accgtccaga ggttgctgcc tgtgctgtgc caagcgcacg
     1741 gactaacccc agagcaggtc gtggcaattg cgagcaacat cgggggaaag caggcactcg
     1801 aaaccgtcca gaggttgctg cctgtgctgt gccaagcgca cgggttgacc ccagagcagg
     1861 tcgtggcaat tgcgagccat gacgggggaa agcaggcact cgaaaccgtc cagaggttgc
     1921 tgcctgtgct gtgccaagcg cacggcctga ccccagagca ggtcgtggca attgcgagca
     1981 acatcggggg aaagcaggca ctcgaaaccg tccagaggtt gctgcctgtg ctgtgccaag
     2041 cgcacggact gacaccagag caggtcgtgg caattgcgag caaccacggg ggaaagcagg
     2101 cactcgaaac cgtccagagg ttgctgcctg tgctgtgcca agcgcacgga cttacacccg
     2161 aacaagtcgt ggcaattgcg agcaacatcg ggggaaagca ggcactcgaa accgtccaga
     2221 ggttgctgcc tgtgctgtgc caagcgcacg gacttacgcc agagcaggtc gtggcaattg
     2281 cgagcaacat cgggggaaag caggcactcg aaaccgtcca gaggttgctg cctgtgctgt
     2341 gccaagcgca cggactaacc ccagagcagg tcgtggcaat tgcgagcaac cacgggggaa
     2401 agcaggcact cgaaaccgtc cagaggttgc tgcctgtgct gtgccaagcg cacgggttga
     2461 ccccagagca ggtcgtggca attgcgagcc atgacggggg aaagcaggca ctcgaaaccg
     2521 tccagaggtt gctgcctgtg ctgtgccaag cgcacggcct gaccccagag caggtcgtgg
     2581 caattgcgag caacatcggg ggaaagcagg cactcgaaac cgtccagagg ttgctgcctg
     2641 tgctgtgcca agcgcacgga ctgacaccag agcaggtcgt ggcaattgcg agcaacggag
     2701 ggggaaagca ggcactcgaa accgtccaga ggttgctgcc tgtgctgtgc caagcgcacg
     2761 gcctcacccc agagcaggtc gtggcaattg cgagcaacgg agggggaaag caggcactcg
     2821 aaaccgtcca gaggttgctg cctgtgctgt gccaagcgca cggacttacg ccagagcagg
     2881 tcgtggcaat tgcgagccat gacgggggaa agcaggcact cgaaaccgtc cagaggttgc
     2941 tgcctgtgct gtgccaagcg cacggactaa ccccagagca ggtcgtggca attgcgagca
     3001 acggaggggg aaagcaggca ctcgaaaccg tccagaggtt gctgcctgtg ctgtgccaag
     3061 cgcacgggtt gaccccagag caggtcgtgg caattgcgag ccatgacggg ggaaagcagg
     3121 cactcgaaac cgtccagagg ttgctgcctg tgctgtgcca agcgcacggc ctgaccccag
     3181 agcaggtcgt ggcaattgcg agcaacatcg ggggaaagca ggcactcgaa accgtccaga
     3241 ggttgctgcc tgtgctgtgc caagcgcacg gactgacacc agagcaggtc gtggcaattg
     3301 cgagcaacca cgggggaaag caggcactcg aaaccgtcca gaggttgctg cctgtgctgt
     3361 gccaagcgca cggactcacg cctgagcagg tagtggctat tgcatccaac atcgggggca
     3421 gacccgcact ggagtcaatc gtggcccagc tttcgaggcc ggaccccgcg ctggccgcac
     3481 tcactaatga tcatcttgta gcgctggcct gcctcggcgg acgacccgcc ttggatgcgg
     3541 tgaagaaggg gctcccgcac gcgcctgcat tgattaagcg gaccaacaga aggattcccg
     3601 agaggacatc acatcgagtg gcaggcctgc agggaagtgg aagtgtgagc aagggcgagg
     3661 agctgttcac cggggtggtg cccatcctgg tcgagctgga cggcgacgta aacggccaca
     3721 agttcagcgt gtccggcgag ggcgagggcg atgccaccta cggcaagctg accctgaagc
     3781 tcatctgcac caccggcaag ctgcccgtgc cctggcccac cctcgtgacc accctcggct
     3841 acggcctgca gtgcttcgcc cgctaccccg accacatgaa gcagcacgac ttcttcaagt
     3901 ccgccatgcc cgaaggctac gtccaggagc gcaccatctt cttcaaggac gacggcaact
     3961 acaagacccg cgccgaggtg aagttcgagg gcgacaccct ggtgaaccgc atcgagctga
     4021 agggcatcga cttcaaggag gacggcaaca tcctggggca caagctggag tacaactaca
     4081 acagccacaa cgtctatatc accgccgaca agcagaagaa cggcatcaag gccaacttca
     4141 agatccgcca caacatcgag gacggcggcg tgcagctcgc cgaccactac cagcagaaca
     4201 cccccatcgg cgacggcccc gtgctgctgc ccgacaacca ctacctgagc taccagtcca
     4261 agctgagcaa agactaatct agagggcccg tttaaacccg ctgatcagcc tcgactgtgc
     4321 cttctagttg ccagccatct gttgtttgcc cctcccccgt gccttccttg accctggaag
     4381 gtgccactcc cactgtcctt tcctaataaa atgaggaaat tgcatcgcat tgtctgagta
     4441 ggtgtcattc tattctgggg ggtggggtgg ggcaggacag caagggggag gattgggaag
     4501 acaatagcag gcatgctggg gatgcggtgg gctctatggc ttctgaggcg gaaagaacca
     4561 gctggggctc tagggggtat ccccacgcgc cctgtagcgg cgcattaagc gcggcgggtg
     4621 tggtggttac gcgcagcgtg accgctacac ttgccagcgc cctagcgccc gctcctttcg
     4681 ctttcttccc ttcctttctc gccacgttcg ccggctttcc ccgtcaagct ctaaatcggg
     4741 ggctcccttt agggttccga tttagtgctt tacggcacct cgaccccaaa aaacttgatt
     4801 agggtgatgg ttcacgtagt gggccatcgc cctgatagac ggtttttcgc cctttgacgt
     4861 tggagtccac gttctttaat agtggactct tgttccaaac tggaacaaca ctcaacccta
     4921 tctcggtcta ttcttttgat ttataaggga ttttgccgat ttcggcctat tggttaaaaa
     4981 atgagctgat ttaacaaaaa tttaacgcga attaattctg tggaatgtgt gtcagttagg
     5041 gtgtggaaag tccccaggct ccccagcagg cagaagtatg caaagcatgc atctcaatta
     5101 gtcagcaacc aggtgtggaa agtccccagg ctccccagca ggcagaagta tgcaaagcat
     5161 gcatctcaat tagtcagcaa ccatagtccc gcccctaact ccgcccatcc cgcccctaac
     5221 tccgcccagt tccgcccatt ctccgcccca tggctgacta atttttttta tttatgcaga
     5281 ggccgaggcc gcctctgcct ctgagctatt ccagaagtag tgaggaggct tttttggagg
     5341 cctaggcttt tgcaaaaagc tcccgggagc ttgtatatcc attttcggat ctgatcagca
     5401 cgtgatgaaa aagcctgaac tcaccgcgac gtctgtcgag aagtttctga tcgaaaagtt
     5461 cgacagcgtc tccgacctga tgcagctctc ggagggcgaa gaatctcgtg ctttcagctt
     5521 cgatgtagga gggcgtggat atgtcctgcg ggtaaatagc tgcgccgatg gtttctacaa
     5581 agatcgttat gtttatcggc actttgcatc ggccgcgctc ccgattccgg aagtgcttga
     5641 cattggggaa ttcagcgaga gcctgaccta ttgcatctcc cgccgtgcac agggtgtcac
     5701 gttgcaagac ctgcctgaaa ccgaactgcc cgctgttctg cagccggtcg cggaggccat
     5761 ggatgcgatc gctgcggccg atcttagcca gacgagcggg ttcggcccat tcggaccgca
     5821 aggaatcggt caatacacta catggcgtga tttcatatgc gcgattgctg atccccatgt
     5881 gtatcactgg caaactgtga tggacgacac cgtcagtgcg tccgtcgcgc aggctctcga
     5941 tgagctgatg ctttgggccg aggactgccc cgaagtccgg cacctcgtgc acgcggattt
     6001 cggctccaac aatgtcctga cggacaatgg ccgcataaca gcggtcattg actggagcga
     6061 ggcgatgttc ggggattccc aatacgaggt cgccaacatc ttcttctgga ggccgtggtt
     6121 ggcttgtatg gagcagcaga cgcgctactt cgagcggagg catccggagc ttgcaggatc
     6181 gccgcggctc cgggcgtata tgctccgcat tggtcttgac caactctatc agagcttggt
     6241 tgacggcaat ttcgatgatg cagcttgggc gcagggtcga tgcgacgcaa tcgtccgatc
     6301 cggagccggg actgtcgggc gtacacaaat cgcccgcaga agcgcggccg tctggaccga
     6361 tggctgtgta gaagtactcg ccgatagtgg aaaccgacgc cccagcactc gtccgagggc
     6421 aaaggaatag cacgtgctac gagatttcga ttccaccgcc gccttctatg aaaggttggg
     6481 cttcggaatc gttttccggg acgccggctg gatgatcctc cagcgcgggg atctcatgct
     6541 ggagttcttc gcccacccca acttgtttat tgcagcttat aatggttaca aataaagcaa
     6601 tagcatcaca aatttcacaa ataaagcatt tttttcactg cattctagtt gtggtttgtc
     6661 caaactcatc aatgtatctt atcatgtctg tataccgtcg acctctagct agagcttggc
     6721 gtaatcatgg tcatagctgt ttcctgtgtg aaattgttat ccgctcacaa ttccacacaa
     6781 catacgagcc ggaagcataa agtgtaaagc ctggggtgcc taatgagtga gctaactcac
     6841 attaattgcg ttgcgctcac tgcccgcttt ccagtcggga aacctgtcgt gccagctgca
     6901 ttaatgaatc ggccaacgcg cggggagagg cggtttgcgt attgggcgct cttccgcttc
     6961 ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat cagctcactc
     7021 aaaggcggta atacggttat ccacagaatc aggggataac gcaggaaaga acatgtgagc
     7081 aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag
     7141 gctccgcccc cctgacgagc atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc
     7201 gacaggacta taaagatacc aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt
     7261 tccgaccctg ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct
     7321 ttctcatagc tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg
     7381 ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc ttatccggta actatcgtct
     7441 tgagtccaac ccggtaagac acgacttatc gccactggca gcagccactg gtaacaggat
     7501 tagcagagcg aggtatgtag gcggtgctac agagttcttg aagtggtggc ctaactacgg
     7561 ctacactaga agaacagtat ttggtatctg cgctctgctg aagccagtta ccttcggaaa
     7621 aagagttggt agctcttgat ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt
     7681 ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc
     7741 tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt
     7801 atcaaaaagg atcttcacct agatcctttt aaattaaaaa tgaagtttta aatcaatcta
     7861 aagtatatat gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg aggcacctat
     7921 ctcagcgatc tgtctatttc gttcatccat agttgcctga ctccccgtcg tgtagataac
     7981 tacgatacgg gagggcttac catctggccc cagtgctgca atgataccgc gagaaccacg
     8041 ctcaccggct ccagatttat cagcaataaa ccagccagcc ggaagggccg agcgcagaag
     8101 tggtcctgca actttatccg cctccatcca gtctattaat tgttgccggg aagctagagt
     8161 aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc attgctacag gcatcgtggt
     8221 gtcacgctcg tcgtttggta tggcttcatt cagctccggt tcccaacgat caaggcgagt
     8281 tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc cgatcgttgt
     8341 cagaagtaag ttggccgcag tgttatcact catggttatg gcagcactgc ataattctct
     8401 tactgtcatg ccatccgtaa gatgcttttc tgtgactggt gagtactcaa ccaagtcatt
     8461 ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg gcgtcaatac gggataatac
     8521 cgcgccacat agcagaactt taaaagtgct catcattgga aaacgttctt cggggcgaaa
     8581 actctcaagg atcttaccgc tgttgagatc cagttcgatg taacccactc gtgcacccaa
     8641 ctgatcttca gcatctttta ctttcaccag cgtttctggg tgagcaaaaa caggaaggca
     8701 aaatgccgca aaaaagggaa taagggcgac acggaaatgt tgaatactca tactcttcct
     8761 ttttaaatat tattgaagca tttatcaggg ttattgtctc atgagcggat acatatttga
     8821 atgtatttag aaaaataaac aaataggggt tccgcgcaca tttccccgaa aagtgccacc
     8881 tgacgtc
//
