LOCUS       sequence-394853-        7936 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  over expression vector for killifish lhb tagged with GFP.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7936)
  AUTHORS   Moses E, Atlan T, Sun X, Franek R, Siddiqui A, Marinov GK, Shifman 
            S, Zucker DM, Oron-Gottesman A, Greenleaf WJ, Cohen E, Ram O, Harel 
            I
  TITLE     The killifish germline regulates longevity and somatic repair in a 
            sex-specific manner.
  JOURNAL   Nat Aging. 2024 Jun;4(6):791-813. doi: 10.1038/s43587-024-00632-0. 
            Epub 2024 May 15.
  PUBMED    38750187
REFERENCE   2  (bases 1 to 7936)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s43587-024-00632-0"; journalName: "Nat Aging"; date: 
            "2024-06"; volume: "4"; issue: "6"; pages: "791-813"
COMMENT     Sequence Label: NFlhb-t2a-GFP
FEATURES             Location/Qualifiers
     source          1..7936
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      23..158
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     polyA_signal    198..319
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(209..228)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     263..282
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     LTR             391..624
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     misc_feature    711..1299
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     CDS             817..828
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     1226..1246
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(join(1315..2028,2029..2031,2032..2034))
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             complement(join(1318..2028,2029..2031,2032..2034))
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(1360..1381)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     1709..1728
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     1968..1989
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     CDS             complement(2041..2094)
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     regulatory      2532..2541
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     complement(2610..2634)
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     promoter        complement(2631..2834)
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     complement(2660..2680)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     enhancer        2835..3138
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     misc_feature    3161..3277
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     CDS             complement(3544..3588)
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    3773..4006
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     misc_feature    4499..4624
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     LTR             4671..4851
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     promoter        complement(4852..5078)
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     primer_bind     complement(5106..5126)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(5106..5124)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(5145..5161)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5145..5161)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(5158..5180)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    5169..5185
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(5193..5199,5200..5217,5218..5223))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    5238..5259
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(5376..5393)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(5547..6135)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(5627..5646)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(6306..7097,7098..7166))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     6929..6948
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(7167..7271)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(7297..7752)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(7434..7455)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     7646..7665
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     7879..7901
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     7893..7910
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     7894..7910
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     join(7920..7936,1..3)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        join(7920..7936,1..2)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 gggcgaattg ggtacgtccc taggcctcca aaaaagcctc ctcactactt ctggaatagc
       61 tcagaggccg aggcggcctc ggcctctgca taaataaaaa aaattagtca gccatggggc
      121 ggagaatggg cggaactggg cggagttagg ggcgggatgg gcggagttag gggcgggata
      181 gctagagcca gacatgataa gatacattga tgagtttgga caaaccacaa ctagaatgca
      241 gtgaaaaaaa tgctttattt gtgaaatttg tgatgctatt gctttatttg taaccattat
      301 aagctgcaat aaacaagttc ctctcactct ctgatattca tttctttgca agttataaat
      361 actgaataat aagatgacat gaactactac tgctagagat tttccacact gactaaaagg
      421 gtctgaggga tctctagtta ccagagtcac acaacagacg ggcacacact acttgaagca
      481 ctcaaggcaa gctttattga ggcttaagca gtgggttccc tagttagcca gagagctccc
      541 aggctcagat ctggtctaac cagagagacc cagtacaagc aaaaagcaga tcttgtcttc
      601 gttgggagtg aattagccct tccagtcccc ccttttcttt taaaaagtgg ctaagatcta
      661 cagctgcctt gtaagtcatt ggtcttaaag gtaccgagct cgaattccag gcggggaggc
      721 ggcccaaagg gagatccgac tcgtctgagg gcgaaggcga agacgcggaa gaggccgcag
      781 agccggcagc aggccgcggg aaggaaggtc cgctggattg agggccgaag ggacgtagca
      841 gaaggacgtc ccgcgcagaa tccaggtggc aacacaggcg agcagccatg gaaaggacgt
      901 cagcttcccc gacaacacca cggaattgtc agtgcccaac agccgagccc ctgtccagca
      961 gcgggcaagg caggcggcga tgagttccgc cgtggcaata gggaggggga aagcgaaagt
     1021 cccggaaagg agctgacagg tggtggcaat gccccaacca gtgggggttg cgtcagcaaa
     1081 cacagtgcac accacgccac gttgcctgac aacgggccac aactcctcat aaagagacag
     1141 caaccaggat ttatacaagg aggagaaaat gaaagccata cgggaagcaa tagcatgata
     1201 caaaggcatt aaagcagcgt atccacatag cgtaaaagga gcaacatagt taagaatacc
     1261 agtcaatctt tcacaaattt tgtaatccag aggttgattg tcgacgcggc cgctttactt
     1321 gtacagctcg tccatgccga gagtgatccc ggcggcggtc acgaactcca gcaggaccat
     1381 gtgatcgcgc ttctcgttgg ggtctttgct cagggcggac tgggtgctca ggtagtggtt
     1441 gtcgggcagc agcacggggc cgtcgccgat gggggtgttc tgctggtagt ggtcggcgag
     1501 ctgcacgctg ccgtcctcga tgttgtggcg gatcttgaag ttcaccttga tgccgttctt
     1561 ctgcttgtcg gccatgatat agacgttgtg gctgttgtag ttgtactcca gcttgtgccc
     1621 caggatgttg ccgtcctcct tgaagtcgat gcccttcagc tcgatgcggt tcaccagggt
     1681 gtcgccctcg aacttcacct cggcgcgggt cttgtagttg ccgtcgtcct tgaagaagat
     1741 ggtgcgctcc tggacgtagc cttcgggcat ggcggacttg aagaagtcgt gctgcttcat
     1801 gtggtcgggg tagcggctga agcactgcac gccgtaggtc agggtggtca cgagggtggg
     1861 ccagggcacg ggcagcttgc cggtggtgca gatgaacttc agggtcagct tgccgtaggt
     1921 ggcatcgccc tcgccctcgc cggacacgct gaacttgtgg ccgtttacgt cgccgtccag
     1981 ctcgaccagg atgggcacca ccccggtgaa cagctcttcg cccttgctca ccattctaga
     2041 agggccggga ttctcttcca cgtcaccgca tgtcagcaga ctgcctctgc cctcgtagta
     2101 aaaggtaatg tcattcatgc agaagtcagg tttcaggctt tcgaaggtgc agtcggacgt
     2161 atccatggag cagcggccac agcggcagct caaagccacg gggtaggtga cgatggggtc
     2221 gactccaggg agacagtcag gaagctcaaa cgtcttgtag gaaaagttct gataggtgca
     2281 cacatgctgg tacactttgc tgaatcgtat gggcccgacg gggtatttgg tgaagcagtg
     2341 cccactgcag atggttgttt ccactggatg acacccgaag cagccctcct tctccagaga
     2401 aatggtctgg ttggtgagct ggcacaaagg cagctggaac gccactgctg gaggaagatg
     2461 ccagaagata gaggaggatc ctaaaagtaa actcaacatc aacgggaata tctctttact
     2521 ggcctgaagc accatggtgg cgaccggtgg atcctctaga gtcggtgtct tctatggagg
     2581 tcaaaacagc gtggatggcg tctccaggcg atctgacggt tcactaaacg agctctgctt
     2641 atatagacct cccaccgtac acgcctaccg cccatttgcg tcaatggggc ggagttgtta
     2701 cgacattttg gaaagtcccg ttgattttgg tgccaaaaca aactcccatt gacgtcaatg
     2761 gggtggagac ttggaaatcc ccgtgagtca aaccgctatc cacgcccatt gatgtactgc
     2821 caaaaccgca tcaccatggt aatagcgatg actaatacgt agatgtactg ccaagtagga
     2881 aagtcccata aggtcatgta ctgggcataa tgccaggcgg gccatttacc gtcattgacg
     2941 tcaatagggg gcgtacttgg catatgatac acttgatgta ctgccaagtg ggcagtttac
     3001 cgtaaatact ccacccattg acgtcaatgg aaagtcccta ttggcgttac tatgggaaca
     3061 tacgtcatta ttgacgtcaa tgggcggggg tcgttgggcg gtcagccagg cgggccattt
     3121 accgtaagtt atgtaacgcg gaactcccaa gcttatcgat aaaattttga attttgtaat
     3181 ttgtttttgt aattctttag tttgtatgtc tgttgctatt atgtctacta ttctttcccc
     3241 tgcactatac cccccaatcc ccccttttct tttaaaagtt aaccgatacc gtcgagatcc
     3301 gttcactaat cgaatggatc tgtctctgtc tctctctcca ccttcttctt ctattccttc
     3361 gggcctgtcg ggtcccctcg gggttgggag gtgggtctga aacgataatg gtgaatatcc
     3421 ctgcctaact ctattcacta tagaaagtac agcaaaaact attcttaaac ctaccaagcc
     3481 tcctactatc attatgaata attttatata ccacagccaa tttgttatgt taaaccaatt
     3541 ccacaaactt gcccatttat ctaattccaa taattcttgt tcattctttt cttgctggtt
     3601 ttgcgattct tcaattaagg agtgtattaa gcttgtgtaa ttgttaattt ctctgtccca
     3661 ctccatccag gtcgtgtgat tccaaatctg ttccagagat ttattactcc aactagcatt
     3721 ccaaggcaca gcagtggtgc aaatgagttt tccagagcaa ccccaaatcc ccaggagctg
     3781 ttgatccttt aggtatcttt ccacagccag gattcttgcc tggagctgct tgatgcccca
     3841 gactgtgagt tgcaacagat gctgttgcgc ctcaatagcc ctcagcaaat tgttctgctg
     3901 ctgcactata ccagacaata attgtctggc ctgtaccgtc agcgtcattg acgctgcgcc
     3961 catagtgctt cctgctgctc ccaagaaccc aaggaacaaa gctcctattc ccactgctct
     4021 tttttctctc tgcaccactc ttctctttgc cttggtgggt gctactccta atggttcaat
     4081 ttttactact ttatatttat ataattcact tctccaattg tccctcatat ctcctcctcc
     4141 aggtctgaag atcagcggcc gcttgctgtg cggtggtctt acttttgttt tgctcttcct
     4201 ctatcttgtc taaagcttcc ttggtgtctt ttatctctat cctttgatgc acacaataga
     4261 gggttgctac tgtattatat aatgatctaa gttcttctga tcctgtctga agggatggtt
     4321 gtagctgtcc cagtatttgt ctacagcctt ctgatgtttc taacaggcca ggattaactg
     4381 cgaatcgttc tagctccctg cttgcccata ctatatgttt taatttatat tttttctttc
     4441 cccctggcct taaccgaatt ttttcccatc gcgatctaat tctcccccgc ttaatactga
     4501 cgctctcgca cccatctctc tccttctagc ctccgctagt caaaattttt ggcgtactca
     4561 ccagtcgccg cccctcgcct cttgccgtgc gcgcttcagc aagccgagtc ctgcgtcgag
     4621 agagctcctc tggtttccct ttcgctttca agtccctgtt cgggcgccac tgctagagat
     4681 tttccacact gactaaaagg gtctgaggga tctctagtta ccagagtcac acaacagacg
     4741 ggcacacact acttgaagca ctcaaggcaa gctttattga ggcttaagca gtgggttccc
     4801 tagttagcca gagagctccc aggctcagat ctggtctaac cagagagacc cgtttatgta
     4861 tcgagctagg cacttaaata caatatctct gcaatgcggc aattcagtgg ttcgtccaat
     4921 ccatgtcaga cccgtctgtt gccttcctaa taaggcacga tcgtaccacc ttacttccac
     4981 caatcggcat gcacggtgct ttttctctcc ttgtaaggca tgttgctaac tcatcgttac
     5041 catgttgcaa gactacaaga gtattgcata agactacatt aagcttgcag ctccagcttt
     5101 tgttcccttt agtgagggtt aattgcgcgc ttggcgtaat catggtcata gctgtttcct
     5161 gtgtgaaatt gttatccgct cacaattcca cacaacatac gagccggaag cataaagtgg
     5221 aaagcctggg gtgcctaatg agtgagctaa ctcacattaa ttgcgttgcg ctcactgccc
     5281 gctttccagt cgggaaacct gtcgtgccag ctgcattaat gaatcggcca acgcgcgggg
     5341 agaggcggtt tgcgtattgg gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg
     5401 gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg cggtaatacg gttatccaca
     5461 gaatcagggg ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac
     5521 cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc gcccccctga cgagcatcac
     5581 aaaaatcgac gctcaagtca gaggtggcga aacccgacag gactataaag ataccaggcg
     5641 tttccccctg gaagctccct cgtgcgctct cctgttccga ccctgccgct taccggatac
     5701 ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc atagctcacg ctgtaggtat
     5761 ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag
     5821 cccgaccgct gcgccttatc cggtaactat cgtcttgagt ccaacccggt aagacacgac
     5881 ttatcgccac tggcagcagc cactggtaac aggattagca gagcgaggta tgtaggcggt
     5941 gctacagagt tcttgaagtg gtggcctaac tacggctaca ctagaagaac agtatttggt
     6001 atctgcgctc tgctgaagcc agttaccttc ggaaaaagag ttggtagctc ttgatccggc
     6061 aaacaaacca ccgctggtag cggtggtttt tttgtttgca agcagcagat tacgcgcaga
     6121 aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc tcagtggaac
     6181 gaaaactcac gttaagggat tttggtcatg agattatcaa aaaggatctt cacctagatc
     6241 cttttaaatt aaaaatgaag ttttaaatca atctaaagta tatatgagta aacttggtct
     6301 gacagttacc aatgcttaat cagtgaggca cctatctcag cgatctgtct atttcgttca
     6361 tccatagttg cctgactccc cgtcgtgtag ataactacga tacgggaggg cttaccatct
     6421 ggccccagtg ctgcaatgat accgcgagac ccacgctcac cggctccaga tttatcagca
     6481 ataaaccagc cagccggaag ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc
     6541 atccagtcta ttaattgttg ccgggaagct agagtaagta gttcgccagt taatagtttg
     6601 cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt tggtatggct
     6661 tcattcagct ccggttccca acgatcaagg cgagttacat gatcccccat gttgtgcaaa
     6721 aaagcggtta gctccttcgg tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta
     6781 tcactcatgg ttatggcagc actgcataat tctcttactg tcatgccatc cgtaagatgc
     6841 ttttctgtga ctggtgagta ctcaaccaag tcattctgag aatagtgtat gcggcgaccg
     6901 agttgctctt gcccggcgtc aatacgggat aataccgcgc cacatagcag aactttaaaa
     6961 gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct caaggatctt accgctgttg
     7021 agatccagtt cgatgtaacc cactcgtgca cccaactgat cttcagcatc ttttactttc
     7081 accagcgttt ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg
     7141 gcgacacgga aatgttgaat actcatactc ttcctttttc aatattattg aagcatttat
     7201 cagggttatt gtctcatgag cggatacata tttgaatgta tttagaaaaa taaacaaata
     7261 ggggttccgc gcacatttcc ccgaaaagtg ccacctaaat tgtaagcgtt aatattttgt
     7321 taaaattcgc gttaaatttt tgttaaatca gctcattttt taaccaatag gccgaaatcg
     7381 gcaaaatccc ttataaatca aaagaataga ccgagatagg gttgagtgtt gttccagttt
     7441 ggaacaagag tccactatta aagaacgtgg actccaacgt caaagggcga aaaaccgtct
     7501 atcagggcga tggcccacta cgtgaaccat caccctaatc aagttttttg gggtcgaggt
     7561 gccgtaaagc actaaatcgg aaccctaaag ggagcccccg atttagagct tgacggggaa
     7621 agccggcgaa cgtggcgaga aaggaaggga agaaagcgaa aggagcgggc gctagggcgc
     7681 tggcaagtgt agcggtcacg ctgcgcgtaa ccaccacacc cgccgcgctt aatgcgccgc
     7741 tacagggcgc gtcccattcg ccattcaggc tgcgcaactg ttgggaaggg cgatcggtgc
     7801 gggcctcttc gctattacgc cagctggcga aagggggatg tgctgcaagg cgattaagtt
     7861 gggtaacgcc agggttttcc cagtcacgac gttgtaaaac gacggccagt gagcgcgcgt
     7921 aatacgactc actata
//
