LOCUS       sequence-394868-        8811 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Allows for in vitro transcription of original 
            pCMV-T7-ABEmax(7.10)-SpG-P2A-EGFP (RTW4562) without EGFP.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8811)
  AUTHORS   Lederer, CW
  TITLE     pCMV-T7-ABEmax editors
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8811)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pCMV-T7-ABEmax-SpG
FEATURES             Location/Qualifiers
     source          1..8811
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        115..318
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     269..289
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     360..379
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        360..378
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             428..448
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             1637..5737
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="DKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
                     LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
                     LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
                     RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE
                     NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
                     GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
                     QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR
                     KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
                     SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
                     TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
                     VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
                     KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
                     LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
                     KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
                     YYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS
                     EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
                     AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
                     AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
                     ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
                     SILPKRNSDKLIARKKDWDPKKYGGFLWPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
                     IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAKQLQKGNEL
                     ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
                     NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKQYRSTKEVLD
                     ATLIHQSITGLYETRIDLSQLGGD"
     CDS             5780..5800
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             5809..5826
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(5849..5866)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    5855..6079
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(6150..6166)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6150..6166)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(6163..6185)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    6174..6190
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(6198..6204,6205..6222,6223..6228))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6243..6264
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(6381..6398)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6552..7140)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6632..6651)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7311..8102,8103..8171))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     7934..7953
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8172..8276)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(8355..8374)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        join(8546..8811,1..114)
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
ORIGIN
        1 cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta catgacctta
       61 tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac catggtgatg
      121 cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg atttccaagt
      181 ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg ggactttcca
      241 aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt acggtgggag
      301 gtctatataa gcagagctgg tttagtgaac cgtcagatcc gctagagatc cgcggccgct
      361 aatacgactc actataggga gagccgccac catgaaacgg acagccgacg gaagcgagtt
      421 cgagtcacca aagaagaagc ggaaagtctc tgaagtcgag tttagccacg agtattggat
      481 gaggcacgca ctgaccctgg caaagcgagc atgggatgaa agagaagtcc ccgtgggcgc
      541 cgtgctggtg cacaacaata gagtgatcgg agagggatgg aacaggccaa tcggccgcca
      601 cgaccctacc gcacacgcag agatcatggc actgaggcag ggaggcctgg tcatgcagaa
      661 ttaccgcctg atcgatgcca ccctgtatgt gacactggag ccatgcgtga tgtgcgcagg
      721 agcaatgatc cacagcagga tcggaagagt ggtgttcgga gcacgggacg ccaagaccgg
      781 cgcagcaggc tccctgatgg atgtgctgca ccaccccggc atgaaccacc gggtggagat
      841 cacagaggga atcctggcag acgagtgcgc cgccctgctg agcgatttct ttagaatgcg
      901 gagacaggag atcaaggccc agaagaaggc acagagctcc accgactctg gaggatctag
      961 cggaggatcc tctggaagcg agacaccagg cacaagcgag tccgccacac cagagagctc
     1021 cggcggctcc tccggaggat cctctgaggt ggagttttcc cacgagtact ggatgagaca
     1081 tgccctgacc ctggccaaga gggcacgcga tgagagggag gtgcctgtgg gagccgtgct
     1141 ggtgctgaac aatagagtga tcggcgaggg ctggaacaga gccatcggcc tgcacgaccc
     1201 aacagcccat gccgaaatta tggccctgag acagggcggc ctggtcatgc agaactacag
     1261 actgattgac gccaccctgt acgtgacatt cgagccttgc gtgatgtgcg ccggcgccat
     1321 gatccactct aggatcggcc gcgtggtgtt tggcgtgagg aacgcaaaaa ccggcgccgc
     1381 aggctccctg atggacgtgc tgcactaccc cggcatgaat caccgcgtcg aaattaccga
     1441 gggaatcctg gcagatgaat gtgccgccct gctgtgctat ttctttcgga tgcctagaca
     1501 ggtgttcaat gctcagaaga aggcccagag ctccaccgac tccggaggat ctagcggagg
     1561 ctcctctggc tctgagacac ctggcacaag cgagagcgca acacctgaaa gcagcggggg
     1621 cagcagcggg gggtcagaca agaagtacag catcggcctg gccatcggca ccaactctgt
     1681 gggctgggcc gtgatcaccg acgagtacaa ggtgcccagc aagaaattca aggtgctggg
     1741 caacaccgac cggcacagca tcaagaagaa cctgatcgga gccctgctgt tcgacagcgg
     1801 cgaaacagcc gaggccaccc ggctgaagag aaccgccaga agaagataca ccagacggaa
     1861 gaaccggatc tgctatctgc aagagatctt cagcaacgag atggccaagg tggacgacag
     1921 cttcttccac agactggaag agtccttcct ggtggaagag gataagaagc acgagcggca
     1981 ccccatcttc ggcaacatcg tggacgaggt ggcctaccac gagaagtacc ccaccatcta
     2041 ccacctgaga aagaaactgg tggacagcac cgacaaggcc gacctgcggc tgatctatct
     2101 ggccctggcc cacatgatca agttccgggg ccacttcctg atcgagggcg acctgaaccc
     2161 cgacaacagc gacgtggaca agctgttcat ccagctggtg cagacctaca accagctgtt
     2221 cgaggaaaac cccatcaacg ccagcggcgt ggacgccaag gccatcctgt ctgccagact
     2281 gagcaagagc agacggctgg aaaatctgat cgcccagctg cccggcgaga agaagaatgg
     2341 cctgttcgga aacctgattg ccctgagcct gggcctgacc cccaacttca agagcaactt
     2401 cgacctggcc gaggatgcca aactgcagct gagcaaggac acctacgacg acgacctgga
     2461 caacctgctg gcccagatcg gcgaccagta cgccgacctg tttctggccg ccaagaacct
     2521 gtccgacgcc atcctgctga gcgacatcct gagagtgaac accgagatca ccaaggcccc
     2581 cctgagcgcc tctatgatca agagatacga cgagcaccac caggacctga ccctgctgaa
     2641 agctctcgtg cggcagcagc tgcctgagaa gtacaaagag attttcttcg accagagcaa
     2701 gaacggctac gccggctaca ttgacggcgg agccagccag gaagagttct acaagttcat
     2761 caagcccatc ctggaaaaga tggacggcac cgaggaactg ctcgtgaagc tgaacagaga
     2821 ggacctgctg cggaagcagc ggaccttcga caacggcagc atcccccacc agatccacct
     2881 gggagagctg cacgccattc tgcggcggca ggaagatttt tacccattcc tgaaggacaa
     2941 ccgggaaaag atcgagaaga tcctgacctt ccgcatcccc tactacgtgg gccctctggc
     3001 caggggaaac agcagattcg cctggatgac cagaaagagc gaggaaacca tcaccccctg
     3061 gaacttcgag gaagtggtgg acaagggcgc ttccgcccag agcttcatcg agcggatgac
     3121 caacttcgat aagaacctgc ccaacgagaa ggtgctgccc aagcacagcc tgctgtacga
     3181 gtacttcacc gtgtataacg agctgaccaa agtgaaatac gtgaccgagg gaatgagaaa
     3241 gcccgccttc ctgagcggcg agcagaaaaa ggccatcgtg gacctgctgt tcaagaccaa
     3301 ccggaaagtg accgtgaagc agctgaaaga ggactacttc aagaaaatcg agtgcttcga
     3361 ctccgtggaa atctccggcg tggaagatcg gttcaacgcc tccctgggca cataccacga
     3421 tctgctgaaa attatcaagg acaaggactt cctggacaat gaggaaaacg aggacattct
     3481 ggaagatatc gtgctgaccc tgacactgtt tgaggacaga gagatgatcg aggaacggct
     3541 gaaaacctat gcccacctgt tcgacgacaa agtgatgaag cagctgaagc ggcggagata
     3601 caccggctgg ggcaggctga gccggaagct gatcaacggc atccgggaca agcagtccgg
     3661 caagacaatc ctggatttcc tgaagtccga cggcttcgcc aacagaaact tcatgcagct
     3721 gatccacgac gacagcctga cctttaaaga ggacatccag aaagcccagg tgtccggcca
     3781 gggcgatagc ctgcacgagc acattgccaa tctggccggc agccccgcca ttaagaaggg
     3841 catcctgcag acagtgaagg tggtggacga gctcgtgaaa gtgatgggcc ggcacaagcc
     3901 cgagaacatc gtgatcgaaa tggccagaga gaaccagacc acccagaagg gacagaagaa
     3961 cagccgcgag agaatgaagc ggatcgaaga gggcatcaaa gagctgggca gccagatcct
     4021 gaaagaacac cccgtggaaa acacccagct gcagaacgag aagctgtacc tgtactacct
     4081 gcagaatggg cgggatatgt acgtggacca ggaactggac atcaaccggc tgtccgacta
     4141 cgatgtggac catatcgtgc ctcagagctt tctgaaggac gactccatcg acaacaaggt
     4201 gctgaccaga agcgacaaga accggggcaa gagcgacaac gtgccctccg aagaggtcgt
     4261 gaagaagatg aagaactact ggcggcagct gctgaacgcc aagctgatta cccagagaaa
     4321 gttcgacaat ctgaccaagg ccgagagagg cggcctgagc gaactggata aggccggctt
     4381 catcaagaga cagctggtgg aaacccggca gatcacaaag cacgtggcac agatcctgga
     4441 ctcccggatg aacactaagt acgacgagaa tgacaagctg atccgggaag tgaaagtgat
     4501 caccctgaag tccaagctgg tgtccgattt ccggaaggat ttccagtttt acaaagtgcg
     4561 cgagatcaac aactaccacc acgcccacga cgcctacctg aacgccgtcg tgggaaccgc
     4621 cctgatcaaa aagtacccta agctggaaag cgagttcgtg tacggcgact acaaggtgta
     4681 cgacgtgcgg aagatgatcg ccaagagcga gcaggaaatc ggcaaggcta ccgccaagta
     4741 cttcttctac agcaacatca tgaacttttt caagaccgag attaccctgg ccaacggcga
     4801 gatccggaag cggcctctga tcgagacaaa cggcgaaacc ggggagatcg tgtgggataa
     4861 gggccgggat tttgccaccg tgcggaaagt gctgagcatg ccccaagtga atatcgtgaa
     4921 aaagaccgag gtgcagacag gcggcttcag caaagagtct atcctgccca agaggaacag
     4981 cgataagctg atcgccagaa agaaggactg ggaccctaag aagtacggcg gcttcctgtg
     5041 gcccaccgtg gcctattctg tgctggtggt ggccaaagtg gaaaagggca agtccaagaa
     5101 actgaagagt gtgaaagagc tgctggggat caccatcatg gaaagaagca gcttcgagaa
     5161 gaatcccatc gactttctgg aagccaaggg ctacaaagaa gtgaaaaagg acctgatcat
     5221 caagctgcct aagtactccc tgttcgagct ggaaaacggc cggaagagaa tgctggcctc
     5281 tgccaagcag ctgcagaagg gaaacgaact ggccctgccc tccaaatatg tgaacttcct
     5341 gtacctggcc agccactatg agaagctgaa gggctccccc gaggataatg agcagaaaca
     5401 gctgtttgtg gaacagcaca agcactacct ggacgagatc atcgagcaga tcagcgagtt
     5461 ctccaagaga gtgatcctgg ccgacgctaa tctggacaaa gtgctgtccg cctacaacaa
     5521 gcaccgggat aagcccatca gagagcaggc cgagaatatc atccacctgt ttaccctgac
     5581 caatctggga gcccctgccg ccttcaagta ctttgacacc accatcgacc ggaagcagta
     5641 cagaagcacc aaagaggtgc tggacgccac cctgatccac cagagcatca ccggcctgta
     5701 cgagacacgg atcgacctgt ctcagctggg aggtgactct ggcggctcaa aaagaaccgc
     5761 cgacggcagc gaattcgagc ccaagaagaa gaggaaagtc taaccggtca tcatcaccat
     5821 caccattgag tttaaacccg ctgatcagcc tcgactgtgc cttctagttg ccagccatct
     5881 gttgtttgcc cctcccccgt gccttccttg accctggaag gtgccactcc cactgtcctt
     5941 tcctaataaa atgaggaaat tgcatcgcat tgtctgagta ggtgtcattc tattctgggg
     6001 ggtggggtgg ggcaggacag caagggggag gattgggaag acaatagcag gcatgctggg
     6061 gatgcggtgg gctctatggc ttctgaggcg gaaagaacca gctggggctc gataccgtcg
     6121 acctctagct agagcttggc gtaatcatgg tcatagctgt ttcctgtgtg aaattgttat
     6181 ccgctcacaa ttccacacaa catacgagcc ggaagcataa agtgtaaagc ctagggtgcc
     6241 taatgagtga gctaactcac attaattgcg ttgcgctcac tgcccgcttt ccagtcggga
     6301 aacctgtcgt gccagctgca ttaatgaatc ggccaacgcg cggggagagg cggtttgcgt
     6361 attgggcgct cttccgcttc ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg
     6421 cgagcggtat cagctcactc aaaggcggta atacggttat ccacagaatc aggggataac
     6481 gcaggaaaga acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg
     6541 ttgctggcgt ttttccatag gctccgcccc cctgacgagc atcacaaaaa tcgacgctca
     6601 agtcagaggt ggcgaaaccc gacaggacta taaagatacc aggcgtttcc ccctggaagc
     6661 tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg gatacctgtc cgcctttctc
     6721 ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta ggtatctcag ttcggtgtag
     6781 gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc
     6841 ttatccggta actatcgtct tgagtccaac ccggtaagac acgacttatc gccactggca
     6901 gcagccactg gtaacaggat tagcagagcg aggtatgtag gcggtgctac agagttcttg
     6961 aagtggtggc ctaactacgg ctacactaga agaacagtat ttggtatctg cgctctgctg
     7021 aagccagtta ccttcggaaa aagagttggt agctcttgat ccggcaaaca aaccaccgct
     7081 ggtagcggtg gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa
     7141 gaagatcctt tgatcttttc tacggggtct gacactcagt ggaacgaaaa ctcacgttaa
     7201 gggattttgg tcatgagatt atcaaaaagg atcttcacct agatcctttt aaattaaaaa
     7261 tgaagtttta aatcaatcta aagtatatat gagtaaactt ggtctgacag ttaccaatgc
     7321 ttaatcagtg aggcacctat ctcagcgatc tgtctatttc gttcatccat agttgcctga
     7381 ctccccgtcg tgtagataac tacgatacgg gagggcttac catctggccc cagtgctgca
     7441 atgataccgc gagacccacg ctcaccggct ccagatttat cagcaataaa ccagccagcc
     7501 ggaagggccg agcgcagaag tggtcctgca actttatccg cctccatcca gtctattaat
     7561 tgttgccggg aagctagagt aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc
     7621 attgctacag gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt cagctccggt
     7681 tcccaacgat caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc
     7741 ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag tgttatcact catggttatg
     7801 gcagcactgc ataattctct tactgtcatg ccatccgtaa gatgcttttc tgtgactggt
     7861 gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg
     7921 gcgtcaatac gggataatac cgcgccacat agcagaactt taaaagtgct catcattgga
     7981 aaacgttctt cggggcgaaa actctcaagg atcttaccgc tgttgagatc cagttcgatg
     8041 taacccactc gtgcacccaa ctgatcttca gcatctttta ctttcaccag cgtttctggg
     8101 tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa taagggcgac acggaaatgt
     8161 tgaatactca tactcttcct ttttcaatat tattgaagca tttatcaggg ttattgtctc
     8221 atgagcggat acatatttga atgtatttag aaaaataaac aaataggggt tccgcgcaca
     8281 tttccccgaa aagtgccacc tgacgtcgac ggatcgggag atcgatctcc cgatccccta
     8341 gggtcgactc tcagtacaat ctgctctgat gccgcatagt taagccagta tctgctccct
     8401 gcttgtgtgt tggaggtcgc tgagtagtgc gcgagcaaaa tttaagctac aacaaggcaa
     8461 ggcttgaccg acaattgcat gaagaatctg cttagggtta ggcgttttgc gctgcttcgc
     8521 gatgtacggg ccagatatac gcgttgacat tgattattga ctagttatta atagtaatca
     8581 attacggggt cattagttca tagcccatat atggagttcc gcgttacata acttacggta
     8641 aatggcccgc ctggctgacc gcccaacgac ccccgcccat tgacgtcaat aatgacgtat
     8701 gttcccatag taacgccaat agggactttc cattgacgtc aatgggtgga gtatttacgg
     8761 taaactgccc acttggcagt acatcaagtg tatcatatgc caagtacgcc c
//
