LOCUS       sequence-394869-        8811 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Allows for in vitro transcription of original 
            pCMV-T7-ABEmax(7.10)-SpRY-P2A-EGFP (RTW5025) without EGFP.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8811)
  AUTHORS   Lederer, CW
  TITLE     pCMV-T7-ABEmax editors
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8811)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pCMV-T7-ABEmax-SpRY
FEATURES             Location/Qualifiers
     source          1..8811
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        165..368
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     319..339
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     410..429
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        410..428
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             478..498
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             1687..5787
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="DKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
                     LIGALLFDSGETAERTRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
                     LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
                     RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE
                     NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
                     GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
                     QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR
                     KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
                     SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
                     TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
                     VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
                     KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
                     LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
                     KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
                     YYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS
                     EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
                     AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
                     AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
                     ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
                     SIRPKRNSDKLIARKKDWDPKKYGGFLWPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
                     IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAKQLQKGNEL
                     ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
                     NLDKVLSAYNKHRDKPIREQAENIIHLFTLTRLGAPRAFKYFDTTIDPKQYRSTKEVLD
                     ATLIHQSITGLYETRIDLSQLGGD"
     CDS             5830..5850
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             5859..5876
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(5899..5916)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    5905..6129
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(6200..6216)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6200..6216)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(6213..6235)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    6224..6240
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(6248..6254,6255..6272,6273..6278))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6293..6314
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(6431..6448)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6602..7190)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6682..6701)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7361..8152,8153..8221))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     7984..8003
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8222..8326)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(8405..8424)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        join(8596..8811,1..164)
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
ORIGIN
        1 aaactgccca cttggcagta catcaagtgt atcatatgcc aagtacgccc cctattgacg
       61 tcaatgacgg taaatggccc gcctggcatt atgcccagta catgacctta tgggactttc
      121 ctacttggca gtacatctac gtattagtca tcgctattac catggtgatg cggttttggc
      181 agtacatcaa tgggcgtgga tagcggtttg actcacgggg atttccaagt ctccacccca
      241 ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg ggactttcca aaatgtcgta
      301 acaactccgc cccattgacg caaatgggcg gtaggcgtgt acggtgggag gtctatataa
      361 gcagagctgg tttagtgaac cgtcagatcc gctagagatc cgcggccgct aatacgactc
      421 actataggga gagccgccac catgaaacgg acagccgacg gaagcgagtt cgagtcacca
      481 aagaagaagc ggaaagtctc tgaagtcgag tttagccacg agtattggat gaggcacgca
      541 ctgaccctgg caaagcgagc atgggatgaa agagaagtcc ccgtgggcgc cgtgctggtg
      601 cacaacaata gagtgatcgg agagggatgg aacaggccaa tcggccgcca cgaccctacc
      661 gcacacgcag agatcatggc actgaggcag ggaggcctgg tcatgcagaa ttaccgcctg
      721 atcgatgcca ccctgtatgt gacactggag ccatgcgtga tgtgcgcagg agcaatgatc
      781 cacagcagga tcggaagagt ggtgttcgga gcacgggacg ccaagaccgg cgcagcaggc
      841 tccctgatgg atgtgctgca ccaccccggc atgaaccacc gggtggagat cacagaggga
      901 atcctggcag acgagtgcgc cgccctgctg agcgatttct ttagaatgcg gagacaggag
      961 atcaaggccc agaagaaggc acagagctcc accgactctg gaggatctag cggaggatcc
     1021 tctggaagcg agacaccagg cacaagcgag tccgccacac cagagagctc cggcggctcc
     1081 tccggaggat cctctgaggt ggagttttcc cacgagtact ggatgagaca tgccctgacc
     1141 ctggccaaga gggcacgcga tgagagggag gtgcctgtgg gagccgtgct ggtgctgaac
     1201 aatagagtga tcggcgaggg ctggaacaga gccatcggcc tgcacgaccc aacagcccat
     1261 gccgaaatta tggccctgag acagggcggc ctggtcatgc agaactacag actgattgac
     1321 gccaccctgt acgtgacatt cgagccttgc gtgatgtgcg ccggcgccat gatccactct
     1381 aggatcggcc gcgtggtgtt tggcgtgagg aacgcaaaaa ccggcgccgc aggctccctg
     1441 atggacgtgc tgcactaccc cggcatgaat caccgcgtcg aaattaccga gggaatcctg
     1501 gcagatgaat gtgccgccct gctgtgctat ttctttcgga tgcctagaca ggtgttcaat
     1561 gctcagaaga aggcccagag ctccaccgac tccggaggat ctagcggagg ctcctctggc
     1621 tctgagacac ctggcacaag cgagagcgca acacctgaaa gcagcggggg cagcagcggg
     1681 gggtcagaca agaagtacag catcggcctg gccatcggca ccaactctgt gggctgggcc
     1741 gtgatcaccg acgagtacaa ggtgcccagc aagaaattca aggtgctggg caacaccgac
     1801 cggcacagca tcaagaagaa cctgatcgga gccctgctgt tcgacagcgg cgaaacagcc
     1861 gagagaaccc ggctgaagag aaccgccaga agaagataca ccagacggaa gaaccggatc
     1921 tgctatctgc aagagatctt cagcaacgag atggccaagg tggacgacag cttcttccac
     1981 agactggaag agtccttcct ggtggaagag gataagaagc acgagcggca ccccatcttc
     2041 ggcaacatcg tggacgaggt ggcctaccac gagaagtacc ccaccatcta ccacctgaga
     2101 aagaaactgg tggacagcac cgacaaggcc gacctgcggc tgatctatct ggccctggcc
     2161 cacatgatca agttccgggg ccacttcctg atcgagggcg acctgaaccc cgacaacagc
     2221 gacgtggaca agctgttcat ccagctggtg cagacctaca accagctgtt cgaggaaaac
     2281 cccatcaacg ccagcggcgt ggacgccaag gccatcctgt ctgccagact gagcaagagc
     2341 agacggctgg aaaatctgat cgcccagctg cccggcgaga agaagaatgg cctgttcgga
     2401 aacctgattg ccctgagcct gggcctgacc cccaacttca agagcaactt cgacctggcc
     2461 gaggatgcca aactgcagct gagcaaggac acctacgacg acgacctgga caacctgctg
     2521 gcccagatcg gcgaccagta cgccgacctg tttctggccg ccaagaacct gtccgacgcc
     2581 atcctgctga gcgacatcct gagagtgaac accgagatca ccaaggcccc cctgagcgcc
     2641 tctatgatca agagatacga cgagcaccac caggacctga ccctgctgaa agctctcgtg
     2701 cggcagcagc tgcctgagaa gtacaaagag attttcttcg accagagcaa gaacggctac
     2761 gccggctaca ttgacggcgg agccagccag gaagagttct acaagttcat caagcccatc
     2821 ctggaaaaga tggacggcac cgaggaactg ctcgtgaagc tgaacagaga ggacctgctg
     2881 cggaagcagc ggaccttcga caacggcagc atcccccacc agatccacct gggagagctg
     2941 cacgccattc tgcggcggca ggaagatttt tacccattcc tgaaggacaa ccgggaaaag
     3001 atcgagaaga tcctgacctt ccgcatcccc tactacgtgg gccctctggc caggggaaac
     3061 agcagattcg cctggatgac cagaaagagc gaggaaacca tcaccccctg gaacttcgag
     3121 gaagtggtgg acaagggcgc ttccgcccag agcttcatcg agcggatgac caacttcgat
     3181 aagaacctgc ccaacgagaa ggtgctgccc aagcacagcc tgctgtacga gtacttcacc
     3241 gtgtataacg agctgaccaa agtgaaatac gtgaccgagg gaatgagaaa gcccgccttc
     3301 ctgagcggcg agcagaaaaa ggccatcgtg gacctgctgt tcaagaccaa ccggaaagtg
     3361 accgtgaagc agctgaaaga ggactacttc aagaaaatcg agtgcttcga ctccgtggaa
     3421 atctccggcg tggaagatcg gttcaacgcc tccctgggca cataccacga tctgctgaaa
     3481 attatcaagg acaaggactt cctggacaat gaggaaaacg aggacattct ggaagatatc
     3541 gtgctgaccc tgacactgtt tgaggacaga gagatgatcg aggaacggct gaaaacctat
     3601 gcccacctgt tcgacgacaa agtgatgaag cagctgaagc ggcggagata caccggctgg
     3661 ggcaggctga gccggaagct gatcaacggc atccgggaca agcagtccgg caagacaatc
     3721 ctggatttcc tgaagtccga cggcttcgcc aacagaaact tcatgcagct gatccacgac
     3781 gacagcctga cctttaaaga ggacatccag aaagcccagg tgtccggcca gggcgatagc
     3841 ctgcacgagc acattgccaa tctggccggc agccccgcca ttaagaaggg catcctgcag
     3901 acagtgaagg tggtggacga gctcgtgaaa gtgatgggcc ggcacaagcc cgagaacatc
     3961 gtgatcgaaa tggccagaga gaaccagacc acccagaagg gacagaagaa cagccgcgag
     4021 agaatgaagc ggatcgaaga gggcatcaaa gagctgggca gccagatcct gaaagaacac
     4081 cccgtggaaa acacccagct gcagaacgag aagctgtacc tgtactacct gcagaatggg
     4141 cgggatatgt acgtggacca ggaactggac atcaaccggc tgtccgacta cgatgtggac
     4201 catatcgtgc ctcagagctt tctgaaggac gactccatcg acaacaaggt gctgaccaga
     4261 agcgacaaga accggggcaa gagcgacaac gtgccctccg aagaggtcgt gaagaagatg
     4321 aagaactact ggcggcagct gctgaacgcc aagctgatta cccagagaaa gttcgacaat
     4381 ctgaccaagg ccgagagagg cggcctgagc gaactggata aggccggctt catcaagaga
     4441 cagctggtgg aaacccggca gatcacaaag cacgtggcac agatcctgga ctcccggatg
     4501 aacactaagt acgacgagaa tgacaagctg atccgggaag tgaaagtgat caccctgaag
     4561 tccaagctgg tgtccgattt ccggaaggat ttccagtttt acaaagtgcg cgagatcaac
     4621 aactaccacc acgcccacga cgcctacctg aacgccgtcg tgggaaccgc cctgatcaaa
     4681 aagtacccta agctggaaag cgagttcgtg tacggcgact acaaggtgta cgacgtgcgg
     4741 aagatgatcg ccaagagcga gcaggaaatc ggcaaggcta ccgccaagta cttcttctac
     4801 agcaacatca tgaacttttt caagaccgag attaccctgg ccaacggcga gatccggaag
     4861 cggcctctga tcgagacaaa cggcgaaacc ggggagatcg tgtgggataa gggccgggat
     4921 tttgccaccg tgcggaaagt gctgagcatg ccccaagtga atatcgtgaa aaagaccgag
     4981 gtgcagacag gcggcttcag caaagagtct atcagaccca agaggaacag cgataagctg
     5041 atcgccagaa agaaggactg ggaccctaag aagtacggcg gcttcctgtg gcccaccgtg
     5101 gcctattctg tgctggtggt ggccaaagtg gaaaagggca agtccaagaa actgaagagt
     5161 gtgaaagagc tgctggggat caccatcatg gaaagaagca gcttcgagaa gaatcccatc
     5221 gactttctgg aagccaaggg ctacaaagaa gtgaaaaagg acctgatcat caagctgcct
     5281 aagtactccc tgttcgagct ggaaaacggc cggaagagaa tgctggcctc tgccaagcag
     5341 ctgcagaagg gaaacgaact ggccctgccc tccaaatatg tgaacttcct gtacctggcc
     5401 agccactatg agaagctgaa gggctccccc gaggataatg agcagaaaca gctgtttgtg
     5461 gaacagcaca agcactacct ggacgagatc atcgagcaga tcagcgagtt ctccaagaga
     5521 gtgatcctgg ccgacgctaa tctggacaaa gtgctgtccg cctacaacaa gcaccgggat
     5581 aagcccatca gagagcaggc cgagaatatc atccacctgt ttaccctgac cagactggga
     5641 gcccctagag ccttcaagta ctttgacacc accatcgacc ccaagcagta cagaagcacc
     5701 aaagaggtgc tggacgccac cctgatccac cagagcatca ccggcctgta cgagacacgg
     5761 atcgacctgt ctcagctggg aggtgactct ggcggctcaa aaagaaccgc cgacggcagc
     5821 gaattcgagc ccaagaagaa gaggaaagtc taaccggtca tcatcaccat caccattgag
     5881 tttaaacccg ctgatcagcc tcgactgtgc cttctagttg ccagccatct gttgtttgcc
     5941 cctcccccgt gccttccttg accctggaag gtgccactcc cactgtcctt tcctaataaa
     6001 atgaggaaat tgcatcgcat tgtctgagta ggtgtcattc tattctgggg ggtggggtgg
     6061 ggcaggacag caagggggag gattgggaag acaatagcag gcatgctggg gatgcggtgg
     6121 gctctatggc ttctgaggcg gaaagaacca gctggggctc gataccgtcg acctctagct
     6181 agagcttggc gtaatcatgg tcatagctgt ttcctgtgtg aaattgttat ccgctcacaa
     6241 ttccacacaa catacgagcc ggaagcataa agtgtaaagc ctagggtgcc taatgagtga
     6301 gctaactcac attaattgcg ttgcgctcac tgcccgcttt ccagtcggga aacctgtcgt
     6361 gccagctgca ttaatgaatc ggccaacgcg cggggagagg cggtttgcgt attgggcgct
     6421 cttccgcttc ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat
     6481 cagctcactc aaaggcggta atacggttat ccacagaatc aggggataac gcaggaaaga
     6541 acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt
     6601 ttttccatag gctccgcccc cctgacgagc atcacaaaaa tcgacgctca agtcagaggt
     6661 ggcgaaaccc gacaggacta taaagatacc aggcgtttcc ccctggaagc tccctcgtgc
     6721 gctctcctgt tccgaccctg ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa
     6781 gcgtggcgct ttctcatagc tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct
     6841 ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc ttatccggta
     6901 actatcgtct tgagtccaac ccggtaagac acgacttatc gccactggca gcagccactg
     6961 gtaacaggat tagcagagcg aggtatgtag gcggtgctac agagttcttg aagtggtggc
     7021 ctaactacgg ctacactaga agaacagtat ttggtatctg cgctctgctg aagccagtta
     7081 ccttcggaaa aagagttggt agctcttgat ccggcaaaca aaccaccgct ggtagcggtg
     7141 gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa gaagatcctt
     7201 tgatcttttc tacggggtct gacactcagt ggaacgaaaa ctcacgttaa gggattttgg
     7261 tcatgagatt atcaaaaagg atcttcacct agatcctttt aaattaaaaa tgaagtttta
     7321 aatcaatcta aagtatatat gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg
     7381 aggcacctat ctcagcgatc tgtctatttc gttcatccat agttgcctga ctccccgtcg
     7441 tgtagataac tacgatacgg gagggcttac catctggccc cagtgctgca atgataccgc
     7501 gagacccacg ctcaccggct ccagatttat cagcaataaa ccagccagcc ggaagggccg
     7561 agcgcagaag tggtcctgca actttatccg cctccatcca gtctattaat tgttgccggg
     7621 aagctagagt aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc attgctacag
     7681 gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt cagctccggt tcccaacgat
     7741 caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc
     7801 cgatcgttgt cagaagtaag ttggccgcag tgttatcact catggttatg gcagcactgc
     7861 ataattctct tactgtcatg ccatccgtaa gatgcttttc tgtgactggt gagtactcaa
     7921 ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg gcgtcaatac
     7981 gggataatac cgcgccacat agcagaactt taaaagtgct catcattgga aaacgttctt
     8041 cggggcgaaa actctcaagg atcttaccgc tgttgagatc cagttcgatg taacccactc
     8101 gtgcacccaa ctgatcttca gcatctttta ctttcaccag cgtttctggg tgagcaaaaa
     8161 caggaaggca aaatgccgca aaaaagggaa taagggcgac acggaaatgt tgaatactca
     8221 tactcttcct ttttcaatat tattgaagca tttatcaggg ttattgtctc atgagcggat
     8281 acatatttga atgtatttag aaaaataaac aaataggggt tccgcgcaca tttccccgaa
     8341 aagtgccacc tgacgtcgac ggatcgggag atcgatctcc cgatccccta gggtcgactc
     8401 tcagtacaat ctgctctgat gccgcatagt taagccagta tctgctccct gcttgtgtgt
     8461 tggaggtcgc tgagtagtgc gcgagcaaaa tttaagctac aacaaggcaa ggcttgaccg
     8521 acaattgcat gaagaatctg cttagggtta ggcgttttgc gctgcttcgc gatgtacggg
     8581 ccagatatac gcgttgacat tgattattga ctagttatta atagtaatca attacggggt
     8641 cattagttca tagcccatat atggagttcc gcgttacata acttacggta aatggcccgc
     8701 ctggctgacc gcccaacgac ccccgcccat tgacgtcaat aatgacgtat gttcccatag
     8761 taacgccaat agggactttc cattgacgtc aatgggtgga gtatttacgg t
//
