LOCUS       sequence-39490-e         643 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expression of the eGFP reporter gene was not detected in the brain 
            and eye..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 643)
  AUTHORS   Portales-Casamar E, Swanson DJ, Liu L, de Leeuw CN, Banks KG, Ho Sui
            SJ, Fulton DL, Ali J, Amirabbasi M, Arenillas DJ, Babyak N, Black 
            SF, Bonaguro RJ, Brauer E, Candido TR, Castellarin M, Chen J, Chen 
            Y, Cheng JC, Chopra V, Docking TR, Dreolini L, D'Souza CA, Flynn EK,
            Glenn R, Hatakka K, Hearty TG, Imanian B, Jiang S, Khorasan-zadeh S,
            Komljenovic I, Laprise S, Liao NY, Lim JS, Lithwick S, Liu F, Liu J,
            Lu M, McConechy M, McLeod AJ, Milisavljevic M, Mis J, O'Connor K, 
            Palma B, Palmquist DL, Schmouth JF, Swanson MI, Tam B, Ticoll A, 
            Turner JL, Varhol R, Vermeulen J, Watkins RF, Wilson G, Wong BK, 
            Wong SH, Wong TY, Yang GS, Ypsilanti AR, Jones SJ, Holt RA, 
            Goldowitz D, Wasserman WW, Simpson EM
  TITLE     A regulatory toolbox of MiniPromoters to drive selective expression 
            in the brain.
  JOURNAL   Proc Natl Acad Sci U S A. 2010 Sep 21. 107(38):16589-94.
  PUBMED    20807748
REFERENCE   2  (bases 1 to 643)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A. 2010 Sep 21. 107(38):16589-94."
COMMENT     Sequence Label: pEMS1366
FEATURES             Location/Qualifiers
     source          1..643
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      2..11
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 accatggtgg cggaagttcc tatacctttt gaagaatagg aacttcggcg cgcccgcagg
       61 gcagcacgta gtaggcagga tggtggcagg ctccaggagg ctccgcgtgc gccaggaagc
      121 aaaggcgcgg tagcaggtgc tagtcaactg cggttgggtt tagcagcctg caagcgccca
      181 gactgagcgg ccgctgcttt aaatcccctc ggaggagcgg ggcggggctg ggcactgctg
      241 accaatcgga gggtggggct gggcgctgag aaggcggagg cggggcccgc agccagactg
      301 ggccgggtgc aactttcgcg gcaactggag cacgcggagc tgcaccctcc gcccagagga
      361 cgccgcgtgt ggaaggaaaa gcggcaagag aattggcacg gggatgagac gtgcacaccc
      421 agtttcctag ggaaaagtac ctgccgacct ctagtaacag caggaaagtc acgcagcggt
      481 gtcgtgttga gagagtttgc acgggggtgg tgggtctggg aggcacacag ggctttcttt
      541 gatcgcaggc aagtctgagc ctgctgggtc ctgaggacag gcgcacaggg tgcaaacctg
      601 ggcaaaacag catctgatta tactgataag tacccggggt tgc
//
