LOCUS       sequence-39497-e         636 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expression of the eGFP reporter gene was not detected in the brain 
            and eye..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 636)
  AUTHORS   Portales-Casamar E, Swanson DJ, Liu L, de Leeuw CN, Banks KG, Ho Sui
            SJ, Fulton DL, Ali J, Amirabbasi M, Arenillas DJ, Babyak N, Black 
            SF, Bonaguro RJ, Brauer E, Candido TR, Castellarin M, Chen J, Chen 
            Y, Cheng JC, Chopra V, Docking TR, Dreolini L, D'Souza CA, Flynn EK,
            Glenn R, Hatakka K, Hearty TG, Imanian B, Jiang S, Khorasan-zadeh S,
            Komljenovic I, Laprise S, Liao NY, Lim JS, Lithwick S, Liu F, Liu J,
            Lu M, McConechy M, McLeod AJ, Milisavljevic M, Mis J, O'Connor K, 
            Palma B, Palmquist DL, Schmouth JF, Swanson MI, Tam B, Ticoll A, 
            Turner JL, Varhol R, Vermeulen J, Watkins RF, Wilson G, Wong BK, 
            Wong SH, Wong TY, Yang GS, Ypsilanti AR, Jones SJ, Holt RA, 
            Goldowitz D, Wasserman WW, Simpson EM
  TITLE     A regulatory toolbox of MiniPromoters to drive selective expression 
            in the brain.
  JOURNAL   Proc Natl Acad Sci U S A. 2010 Sep 21. 107(38):16589-94.
  PUBMED    20807748
REFERENCE   2  (bases 1 to 636)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A. 2010 Sep 21. 107(38):16589-94."
COMMENT     Sequence Label: pEMS1372
FEATURES             Location/Qualifiers
     source          1..636
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      2..11
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 accatggtgg cggaagttcc tatacctttt gaagaatagg aacttcggcg cgccacgcgt
       61 gacgggaagc ccttggagcg cgccggagag gctgggaaga gcagagactg gtgacccgga
      121 cctcggagga gctccgccgc cgcagccgct ggtgctactg ctgcggcctc cactagggcc
      181 cctactttat atggaagccc gggcgggggg gctggggggc tcttctatcg cggaccagag
      241 cgctctgacg tctccctata gctaaccggg gcgctgggac gcccggtccg gaaaaccatc
      301 catctccccg ccgagtgatg aaaaggctga gtgtacattg ccccctcttt tttcctcgta
      361 gccccgtccg ccttccccac atcggattaa ttcctccggt cctcgacgcc ttccatccaa
      421 cacaatttag tgaactcctc cctctccagg ctcgccggcg cctccagcct tagctgcctt
      481 gagagtcctg gagagggcga ccccgctgcc tgatctgggc cgggaggggt ggggggtgta
      541 gctggagacg ggtgagctct aaagatggat gggctttgct ggagggattt ggaaccacca
      601 tttgaaggcc ttctgattct ccagaccttt ctcttc
//
