LOCUS       sequence-394986-        1054 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  SPAX8-related truncated mutation (K41*) containing the amino acids 1
            to 40 of human NKX6-2, fused with the Venus fluorescent protein..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1054)
  AUTHORS   Ferreira-Peralta P, Franca B, Murtinheira F, Rodrigues MS, Herrera F
  TITLE     Is spastic ataxia 8 a protein misfolding disorder?
  JOURNAL   Biochim Biophys Acta Mol Basis Dis. 2023 Sep 9;1870(1):166882. doi: 
            10.1016/j.bbadis.2023.166882.
  PUBMED    37696463
REFERENCE   2  (bases 1 to 1054)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Biochim 
            Biophys Acta Mol Basis Dis."; date: "2023-09-9"; volume: 
            "1870(1):166882. doi"; pages: " 10.1016/j.bbadis.2023.166882"
COMMENT     Sequence Label: pcDNA3.1 NKX6-2(1-40)-Venus
FEATURES             Location/Qualifiers
     source          1..1054
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(465..484)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     complement(913..930)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    919..1030
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
ORIGIN
        1 acacaaacag acctggcgcc tttgtgctgt ctagcgctcc tctggccgct ctgcacaata
       61 tggccgagat gaagaccagc ctgtttccat acgctctgca gggacctgcc ggctttaagc
      121 ttggcggagg tggaagcggc ggaggcggat ctggtaccat ggtttctaag ggcgaagaac
      181 tgttcaccgg cgtggtgcct atcctggtgg aactggatgg ggatgtgaac ggccacaagt
      241 tctctgtgtc tggcgaaggc gaaggggatg ccacatacgg caagctgacc ctgaagctga
      301 tctgcaccac aggcaaactg cccgtgcctt ggcctacact ggtcacaaca ctcggctacg
      361 gcctgcagtg ctttgccaga tatcccgacc acatgaagca gcacgatttc ttcaagagcg
      421 ccatgcctga gggctacgtg caagagcgga ccatcttctt caaggacgac ggcaactaca
      481 agaccagggc cgaagtgaag ttcgagggcg acaccctggt caacagaatc gagctgaagg
      541 gcatcgactt taaagaggac ggcaacatcc tgggccacaa gctcgagtac aactacaaca
      601 gccacaacgt gtacatcacc gccgacaagc agaagaacgg catcaaggcc aacttcaaga
      661 tccggcacaa catcgaggat ggcggcgtgc agctggccga tcactatcag cagaacaccc
      721 ctatcggaga tggccccgtc ctgctgcctg ataaccacta cctgagctac cagtctgccc
      781 tgagcaagga ccccaatgag aagagggatc acatggtgct gctggaattc gtgacagccg
      841 ccggaatcac actcggcatg gacgagctgt acaaatgagg atcctaaggg cccgtttaaa
      901 cccgctgatc agcctcgact gtgccttcta gttgccagcc atctgttgtt tgcccctccc
      961 ccgtgccttc cttgaccctg gaaggtgcca ctcccactgt cctttcctaa taaaatgagg
     1021 aaattgcatc gcattgtctg agtaggtgtc attc
//
