LOCUS       sequence-394989-        1073 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  SPAX8-related truncated mutation (E189*) containing the amino acids 
            1 to 188 of human NKX6-2 fused with the Venus fluorescent protein.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1073)
  AUTHORS   Ferreira-Peralta P, Franca B, Murtinheira F, Rodrigues MS, Herrera F
  TITLE     Is spastic ataxia 8 a protein misfolding disorder?
  JOURNAL   Biochim Biophys Acta Mol Basis Dis. 2023 Sep 9;1870(1):166882. doi: 
            10.1016/j.bbadis.2023.166882.
  PUBMED    37696463
REFERENCE   2  (bases 1 to 1073)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Biochim 
            Biophys Acta Mol Basis Dis."; date: "2023-09-9"; volume: 
            "1870(1):166882. doi"; pages: " 10.1016/j.bbadis.2023.166882"
COMMENT     Sequence Label: pcDNA3.1 NKX6-2(1-188)-Venus
FEATURES             Location/Qualifiers
     source          1..1073
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(904..923)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
ORIGIN
        1 aacagacctg gcgcctttgt gctgtctagc gctcctctgg ccgctctgca caatatggcc
       61 gagatgaaga ccagcctgtt tccatacgct ctgcagggac ctgccggctt taaggctcct
      121 gctcttggag gactgggagc acaactgcct ctgggaacac ctcacggcat cagcgatatt
      181 ctgggcagac ctgttggagc tgctggcgga ggattgctcg gaggactgcc tagactgaat
      241 ggactggctt cttctgccgg cgtgtacttt ggacctgctg ctgccgttgc cagaggctat
      301 cctaaacctc tggctgagct tcccggcaga cctcctattt tttggcctgg tgttgtgcag
      361 ggcgctcctt ggagagatcc tagacttgct ggaccagctc ctgcaggcgg agtgctggat
      421 aaggatggca agaagaagca cagcagaccc accttcagcg gccagcagat ctttgccctg
      481 gaaaagacct tcgagcagac caagtatctg gctggccctg agagagccag actggcctat
      541 tctctgggca tgaccaagct tggcggaggt ggaagcggcg gaggcggatc tggtaccatg
      601 gtttctaagg gcgaagaact gttcaccggc gtggtgccta tcctggtgga actggatggg
      661 gatgtgaacg gccacaagtt ctctgtgtct ggcgaaggcg aaggggatgc cacatacggc
      721 aagctgaccc tgaagctgat ctgcaccaca ggcaaactgc ccgtgccttg gcctacactg
      781 gtcacaacac tcggctacgg cctgcagtgc tttgccagat atcccgacca catgaagcag
      841 cacgatttct tcaagagcgc catgcctgag ggctacgtgc aagagcggac catcttcttc
      901 aaggacgacg gcaactacaa gaccagggcc gaagtgaagt tcgagggcga caccctggtc
      961 aacagaatcg agctgaaggg catcgacttt aaagaggacg gcaacatcct gggccacaag
     1021 ctcgagtaca actacaacag ccacaacgtg tacatcaccg ccgacaagca gaa
//
