LOCUS       sequence-39503-e        6926 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6926)
  AUTHORS   Kobayashi T, Antar AA, Boehme KW, Danthi P, Eby EA, Guglielmi KM, 
            Holm GH, Johnson EM, Maginnis MS, Naik S, Skelton WB, Wetzel JD, 
            Wilson GJ, Chappell JD, Dermody TS
  TITLE     A plasmid-based reverse genetics system for animal double-stranded 
            RNA viruses.
  JOURNAL   Cell Host Microbe. 2007 Apr 19;1(2):147-57.
  PUBMED    18005692
REFERENCE   2  (bases 1 to 6926)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell Host 
            Microbe."; date: "2007-04-19"; volume: "1(2)"; pages: "147-57"
COMMENT     Sequence Label: pT7-L1T3D
FEATURES             Location/Qualifiers
     source          1..6926
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(4027..4045)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      4041..4088
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     promoter        complement(4156..4174)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(4157..4174)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(4465..4484)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     primer_bind     complement(4695..4714)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     4814..4836
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(4874..4892)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        4960..5064
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5065..5133,5134..5925)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5283..5302)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      6096..6684
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6585..6604
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     6838..6855
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 gggtaatacg actcactata gctacacgtt ccacttccac gacaatgtca tccatgatac
       61 tgactcagtt tggaccgttc attgagagca tttcaggtat cactgatcaa tcgaatgacg
      121 tgtttgaaga tgcagcaaaa gcattctcta tgtttactcg cagcgatgtc tacaaggcgc
      181 tggatgaaat acctttctct gatgatgcga tgcttccaat ccctccaact atatatacga
      241 aaccatctca cgattcatat tattacattg atgctctaaa ccgtgtgcgt cgcaaaacat
      301 atcagggccc tgatgacgtg tacgtaccta attgttctat tgttgaattg ctggagccac
      361 atgagactct gacatcttat gggcggttgt ccgaggccat cgagaatcgt gccaaggatg
      421 gggacagcca agccagaatc gccacaacgt atggtagaat cgctgaatct caagctcgac
      481 agattaaggc tccattggag aagtttgtgt tggcactatt agtggccgaa gcaggggggt
      541 ctttatatga tccagttttg cagaagtatg atgagattcc agatctatcg cataattgcc
      601 ctttatggtg ttttagagag atctgtcgtc acatatctgg tccattacca gatcgggcac
      661 cttatcttta cttatctgca ggggttttct ggttaatgtc accacgaatg acgtctgcaa
      721 tccctccgct actatccgat cttgttaatt tagctatttt gcaacaaact gcgggtttag
      781 atccatcatt agtgaaattg ggagtacaga tatgccttca tgcagcagct agctcaagtt
      841 atgcatggtt tatcttaaag actaagtcta tttttcctca aaacacgttg cacagtatgt
      901 atgaatctct agaaggggga tactgtccta atcttgaatg gttagagcct agatcagact
      961 ataagttcat gtacatggga gtcatgccat tgtccgctaa gtatgctagg tcggcgccgt
     1021 ccaatgataa gaaagcgcgg gaacttggcg agaaatatgg actgagctca gtcgtcggtg
     1081 agcttcgtaa acggacaaag acgtatgtta aacatgactt tgcttcagtg aggtacattc
     1141 gtgacgctat ggcatgtact agcggtattt tcttggtaag aacacccacc gaaacggtat
     1201 tgcaagaata tacgcagagt ccggagatta aggttcccat tccccagaaa gactggacag
     1261 gcccaatagg tgaaatcaga attctaaaag atacaacaag ttccatcgcg cgttacttat
     1321 atagaacatg gtacttggca gcggcgagaa tggcggctca accacgtacg tgggatccat
     1381 tgtttcaagc gattatgaga tctcaatacg tgacagctag gggtggatct ggcgcagcac
     1441 tccgcgaatc tttgtatgcg atcaatgtgt cgttacctga tttcaagggc ttaccagtga
     1501 aggcagcaac taagatattc caggcggcac aattagcgaa cttgccgttc tcccacacat
     1561 cagtggctat actagctgac acttcaatgg gattgcgaaa tcaggtgcag aggcggccac
     1621 gatccattat gccattaaat gtgccccagc agcaggtttc ggcgccccat acattgacag
     1681 cggattacat taactaccac atgaatctat caaccacgtc tggtagtgcg gtcattgaga
     1741 aggtgattcc tttaggtgta tacgcttcga gccctcctaa ccagtcgatc aacattgaca
     1801 tatctgcgtg tgacgctagt attacttggg atttctttct gtcagtgatt atggcggcta
     1861 tacacgaagg tgtcgctagt agctccattg gaaaaccatt tatgggggtt cctgcatcca
     1921 ttgtaaatga tgagtctgtc gttggagtga gagctgctag gccgatatcg ggaatgcaga
     1981 acatgattca gcatctatcg aaactatata aacgtggatt ttcatataga gtaaacgatt
     2041 ctttttctcc aggtaacgat tttactcata tgactaccac tttcccgtca ggttcaacag
     2101 ccacctctac tgagcatact gctaataata gtacgatgat ggaaactttc ctgacagtat
     2161 ggggacccga acatactgac gaccctgacg tcttacgttt aatgaagtct ttaactattc
     2221 aaagaaatta cgtatgtcaa ggtgatgatg gattaatgat tatcgatggg actactgctg
     2281 gtaaggtgaa cagtgaaact attcagaaga tgctagaatt aatctcaaaa tatggtgagg
     2341 aattcggatg gaaatatgac atagcgtacg atgggactgc cgaatactta aagctatact
     2401 tcatatttgg ctgtcgaatt ccaaatctta gtcgccatcc aatcgtgggg aaagaacggg
     2461 cgaattcttc agcagaggag ccatggccag caattctaga tcagattatg ggtgtcttct
     2521 ttaatggtgt tcatgatggg ttacagtggc agcggtggat acgttattca tgggctctat
     2581 gctgtgcttt ctcacgtcaa agaacaatga ttggtgagag cgtgggttac cttcaatatc
     2641 ctatgtggtc ttttgtctac tggggattac cactggttaa agcgtttggg tcagacccat
     2701 ggatattttc ttggtacatg cctactggag atctgggaat gtatagttgg attagcttga
     2761 tacgccctct gatgacaaga tggatggtgg ctaatggtta cgtaactgac agatgctcac
     2821 ccgtattcgg gaacgcagat tatcgcaggt gtttcaatga acttaaacta tatcaaggtt
     2881 attatatggc acaattgccc aggaatccta agaagtctgg acgagcggcc cctcgggagg
     2941 taagagaaca attcactcag gcattatccg actatctaat gcaaaatcca gaactgaagt
     3001 cacgtgtgct acgtggtcgt agtgagtggg agaaatatgg agcggggata attcacaatc
     3061 ctccgtcatt attcgatgtg ccccataaat ggtatcaggg tgcgcaagag gcagcaatcg
     3121 ctacgagaga agagctggca gaaatggatg agacattaat gcgcgctcga aggcacagct
     3181 attcgaactt ttcaaagtta ttagaggcgt atctgctcgt gaaatggcga atgtgcgagg
     3241 cccgcgaacc gtcggttgat ttgcgattac cattatgtgc gggtattgac ccattaaact
     3301 cagatccttt tctcaagatg gtaagcgttg gaccaatgct ccagagtacg agaaagtact
     3361 ttgctcagac actattcatg gcaaagacgg tgtcgggtct tgacgttaac gcgattgata
     3421 gcgcgttatt acgactgcga acattaggtg ctgataagaa agcattaacg gcgcagttat
     3481 taatggtggg gcttcaggag tcagaagcgg acgcattggc cgggaagata atgctacagg
     3541 atgtgaatac tgtgcaatta gccagagtgg ttaacttagc tgtgccagat acttggatgt
     3601 cgttagactt tgactctatg ttcaaacacc acgtcaagct gcttcccaaa gatggacgtc
     3661 atctaaatac tgatattcct cctcgaatgg gatggttacg ggccatttta cgattcttag
     3721 gtgccggaat ggtaatgact gcgactggag ttgctgtcga catctatctg gaggatatac
     3781 atggcggtgg tcggtcactt ggacagagat tcatgacttg gatgcgacag gaaggacggt
     3841 cagcgtgagt ctaccatggg tcgtggtgcg tcaactcatc gggtcggcat ggcatctcca
     3901 cctcctcgcg gtccgacctg ggcatccgaa ggaggacgtc gtccactcgg atggctaagg
     3961 gagagctcaa aaaaaaggat ccggctgcta acaaagcccg aaaggaagct gagttggctg
     4021 ctgccaccgc tgagcaataa ctagcataac cccttggggc ctctaaacgg gtcttgaggg
     4081 gttttttgct gaaaggagga actatatccg gatcgagatc ctctagagtc gacctgcagg
     4141 catgcaagct tgtattctat agtgtcacct aaatcgtatg tgtatgatac ataaggttat
     4201 gtattaattg tagccgcgtt ctaacgacaa tatgtacaag cctaattgtg tagcatctgg
     4261 cttactgaag cagaccctat catctctctc gtaaactgcc gtcagagtcg gtttggttgg
     4321 acgaaccttc tgagtttctg gtaacgccgt tccgcacccc ggaaatggtc agcgaaccaa
     4381 tcagcagggt catcgctagc cagatcctct acgccggacg catcgtggcc ggcatcaccg
     4441 gcgccacagg tgcggttgct ggcgcctata tcgccgacat caccgatggg gaagatcggg
     4501 ctcgccactt cgggctcatg agcgcttgtt tcggcgtggg tatggtggca ggccccgtgg
     4561 ccgggggact gttgggcgcc atctccttgc accattcctt gcggcggcgg tgctcaacgg
     4621 cctcaaccta ctactgggct gcttcctaat gcaggagtcg cataagggag agcgtcgata
     4681 tggtgcactc tcagtacaat ctgctctgat gccgcatagt taagccagcc ccgacacccg
     4741 ccaacacccg ctgacgcgcc ctgacgggct tgtctgctcc cggcatccgc ttacagacaa
     4801 gctgtgaccg tctccgggag ctgcatgtgt cagaggtttt caccgtcatc accgaaacgc
     4861 gcgagacgaa agggcctcgt gatacgccta tttttatagg ttaatgtcat gataataatg
     4921 gtttcttaga cgtcaggtgg cacttttcgg ggaaatgtgc gcggaacccc tatttgttta
     4981 tttttctaaa tacattcaaa tatgtatccg ctcatgagac aataaccctg ataaatgctt
     5041 caataatatt gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc ccttattccc
     5101 ttttttgcgg cattttgcct tcctgttttt gctcacccag aaacgctggt gaaagtaaaa
     5161 gatgctgaag atcagttggg tgcacgagtg ggttacatcg aactggatct caacagcggt
     5221 aagatccttg agagttttcg ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt
     5281 ctgctatgtg gcgcggtatt atcccgtatt gacgccgggc aagagcaact cggtcgccgc
     5341 atacactatt ctcagaatga cttggttgag tactcaccag tcacagaaaa gcatcttacg
     5401 gatggcatga cagtaagaga attatgcagt gctgccataa ccatgagtga taacactgcg
     5461 gccaacttac ttctgacaac gatcggagga ccgaaggagc taaccgcttt tttgcacaac
     5521 atgggggatc atgtaactcg ccttgatcgt tgggaaccgg agctgaatga agccatacca
     5581 aacgacgagc gtgacaccac gatgcctgta gcaatggcaa caacgttgcg caaactatta
     5641 actggcgaac tacttactct agcttcccgg caacaattaa tagactggat ggaggcggat
     5701 aaagttgcag gaccacttct gcgctcggcc cttccggctg gctggtttat tgctgataaa
     5761 tctggagccg gtgagcgtgg gtctcgcggt atcattgcag cactggggcc agatggtaag
     5821 ccctcccgta tcgtagttat ctacacgacg gggagtcagg caactatgga tgaacgaaat
     5881 agacagatcg ctgagatagg tgcctcactg attaagcatt ggtaactgtc agaccaagtt
     5941 tactcatata tactttagat tgatttaaaa cttcattttt aatttaaaag gatctaggtg
     6001 aagatccttt ttgataatct catgaccaaa atcccttaac gtgagttttc gttccactga
     6061 gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta
     6121 atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa
     6181 gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact
     6241 gtccttctag tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca
     6301 tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt
     6361 accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg
     6421 ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag
     6481 cgtgagctat gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta
     6541 agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat
     6601 ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg
     6661 tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc
     6721 ttttgctggc cttttgctca catgttcttt cctgcgttat cccctgattc tgtggataac
     6781 cgtattaccg cctttgagtg agctgatacc gctcgccgca gccgaacgac cgagcgcagc
     6841 gagtcagtga gcgaggaagc ggaagagcgc ccaatacgca aaccgcctct ccccgcgcgt
     6901 tggccgattc attaatgcag ggggat
//
