LOCUS       sequence-39506-e        6981 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6981)
  AUTHORS   Kobayashi T, Antar AA, Boehme KW, Danthi P, Eby EA, Guglielmi KM, 
            Holm GH, Johnson EM, Maginnis MS, Naik S, Skelton WB, Wetzel JD, 
            Wilson GJ, Chappell JD, Dermody TS
  TITLE     A plasmid-based reverse genetics system for animal double-stranded 
            RNA viruses.
  JOURNAL   Cell Host Microbe. 2007 Apr 19;1(2):147-57.
  PUBMED    18005692
REFERENCE   2  (bases 1 to 6981)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell Host 
            Microbe."; date: "2007-04-19"; volume: "1(2)"; pages: "147-57"
COMMENT     Sequence Label: pT7-L3T3D
FEATURES             Location/Qualifiers
     source          1..6981
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(4082..4100)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      4096..4143
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     promoter        complement(4211..4229)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(4212..4229)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(4520..4539)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     primer_bind     complement(4750..4769)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     4869..4891
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(4929..4947)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        5015..5119
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5120..5188,5189..5980)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5338..5357)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      6151..6739
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6640..6659
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     6893..6910
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 atcgatcccg ggaattctaa tacgactcac tatagctaat cgtcaggatg aagcggattc
       61 caaggaagac aaagggcaaa tccagcggaa agggcaatga ctcaacagag agagcggacg
      121 atggctcgag ccaattaaga gacaagcaaa acaataaggc tggccccgcc actacggagc
      181 ctggcacatc caaccgagag caatacaaag ctcgaccagg tattgcatct gtgcagaggg
      241 ccactgaaag tgcagaaatg cccatgaaga ataatgacga agggacgcca gataagaaag
      301 gaaatactaa gggcgaccta gttaatgagc atagtgaggc taaagacgag gcggatgaag
      361 cgacgaagaa gcaggcaaag gatacagaca aaagtaaagc gcaagtcaca tattcagaca
      421 ctggtatcaa taatgctaat gaactgtcaa gatctgggaa tgtggataat gagggtggaa
      481 gtaatcagaa gccgatgtct accagaatag ctgaggcaac gtctgctata gtgtcgaaac
      541 atcctgcgcg tgttgggctg ccacctaccg ctagcagtgg tcatgggtat cagtgccatg
      601 tctgttctgc agtcctgttt agtcctttag acctagatgc ccacgtcgcc tcacatggtt
      661 tgcatggtaa catgacatta acatcgagtg atatccagcg acatataact gagttcatca
      721 gctcatggca aaatcatcct attgttcaag tttcggctga tgtcgaaaat aagaaaactg
      781 ctcaattgct tcacgctgac actcctcgac tcgtcacttg ggatgctggt ttgtgtactt
      841 cattcaaaat cgtcccgatt gtgccagctc aggtgccgca ggatgtactg gcctatacgt
      901 ttttcacctc ttcatacgct atccaatcac cgtttccaga ggcggcagtg tctaggattg
      961 tggtgcatac gagatgggca tctaatgttg actttgaccg agactcgtct gtcatcatgg
     1021 cgccacctac agaaaacaat atccatttgt ttaaacagtt actaaatact gaaaccctgt
     1081 ctgtaagggg ggctaatccg ctaatgttca gggcgaatgt gttgcatatg ttgctagagt
     1141 tcgtattaga taacttgtat ctgaacagac atacgggatt ctctcaagac cacacgccat
     1201 ttactgaggg tgctaatttg cgttcacttc ctggccccga tgctgagaaa tggtactcga
     1261 ttatgtatcc aacgcgcatg ggaacgccga atgtatccaa aatatgtaat ttcgtcgcct
     1321 cttgtgtgcg aaatcgggtt ggacggtttg atcgagcaca gatgatgaac ggagctatgt
     1381 cagagtgggt ggatgtcttc gagacttcag acgcgctaac cgtctccatt cgaggtcgat
     1441 ggatggctag actagctcgc atgaacataa atccaacaga gatcgaatgg gcattgactg
     1501 aatgtgcaca aggatatgtg actgtcacaa gtccttacgc tcctatcgta aatagattga
     1561 tgccctatcg tatctccaac gctgagcggc aaatatcaca gataatcagg atcatgaaca
     1621 ttggcaataa cgcgacggtg atacaacctg ttctgcaaga tatttcggta ctccttcaac
     1681 gcatatcacc actccaaata gatccaacta ttatttccaa cactatgtca acagtctcgg
     1741 agtctactac tcagaccctc agccccgcgt cctcaatttt gggtaaacta cgaccaagca
     1801 actcagattt ttctagtttt agagtcgcgt tggctggatg gctttataat ggggttgtga
     1861 cgacggtgat tgatgatagt tcatatccaa aagacggcgg cagcgtgacc tcacttgaaa
     1921 atctgtggga tttcttcatc cttgcgcttg ctctaccact gacaactgac ccctgtgcac
     1981 ctgtgaaagc attcatgacc ctagccaaca tgatggttgg tttcgagaca atccctatgg
     2041 ataatcagat ctatactcaa tcgagacgcg cgagtgcttt ctcaacgcct cacacgtggc
     2101 cacgatgctt tatgaacatc cagttaattt ctccaatcga cgctcccatc ttgcgacagt
     2161 gggctgaaat tattcataga tactggccta acccttcaca gattcgttat ggtgcaccga
     2221 acgttttcgg ctcggcaaat ttgttcactc cacctgaggt gctgttattg ccaatcgatc
     2281 atcaaccagc taatgtaaca acgccaacgc tggacttcac caatgagtta actaattggc
     2341 gcgctcgtgt ctgtgagctt atgaagaatc tcgttgataa tcaaagatat caacctggat
     2401 ggacacaaag tctagtctcg tcaatgcgcg gaacgctaga caaattgaag ttgattaaat
     2461 cgatgacacc aatgtatctg caacagctgg ctccggtaga gttagcagtg atagctccca
     2521 tgttgccttt tccacctttc caggtgccat acgtccgtct cgatcgtgac agagttccaa
     2581 caatggttgg agtaacacga cagtcacgag atactattac tcagccggcg ctatcgctgt
     2641 cgacaaccaa tactactgtt ggcgtgccac tagctctaga cgcgagggct atcaccgttg
     2701 cgctgttgtc agggaaatat ccgccggatt tggtgacaaa tgtatggtac gctgatgcca
     2761 tttacccaat gtatgcagac acggaggtgt tctctaatct tcagagagac atgattacct
     2821 gcgaggccgt gcagacatta gtgactctgg tggcgcaaat atcagagacc cagtatcctg
     2881 tagataggta tcttgattgg atcccatcac tgagagcatc ggcggcgacg gcggcgacat
     2941 ttgctgagtg ggttaatact tcaatgaaga cggcgtttga tttgtctgat atgctgttag
     3001 agcctctcct aagcggtgat ccgaggatga ctcaactagc gattcagtat cagcagtaca
     3061 atggcagaac gtttaatatc atacctgaaa tgccaggttc agtaattgct gactgcgttc
     3121 aattaacagc agaagtcttt aatcacgaat ataacctgtt tgggattgcg cggggtgata
     3181 tcatcattgg ccgtgttcag tcgacacatt tgtggtcacc gctggctcct ccacctgacc
     3241 tggtgtttga tcgtgatacc cctggtgttc acatcttcgg acgagattgc cgtatatcgt
     3301 ttggaatgaa tggcgccgcg ccaatgatta gagatgagac tggactgatg gtgccttttg
     3361 aaggaaattg gattttccca ctggcgcttt ggcaaatgaa tacacgatat tttaatcaac
     3421 agttcgacgc gtggattaag acaggagagt tgcgaatccg cattgagatg ggcgcgtatc
     3481 catatatgtt gcattactat gatccacgtc agtacgctaa tgcatggaat ttaacatccg
     3541 cctggcttga agaaattacg ccgacgagca tcccatccgt gcctttcatg gtgcccattt
     3601 caagtgatca tgacatttcc tctgccccag ctgtccaata tatcatttca actgaatata
     3661 atgatcggtc tctgttctgc actaactcat catctcccca aaccatcgct ggaccagaca
     3721 aacacattcc agttgagaga tataacattc tgaccaaccc cgacgctcca cccacgcaga
     3781 tacaactgcc tgaagtcgtt gacttgtaca acgtcgtcac acgctatgcg tatgagactc
     3841 cgcctattac cgctgttgtt atgggtgttc cttgatcctc atcctcccaa caggtgctag
     3901 agcattgcgc tcaatgctag ttgggccgat tcatcgggtc ggcatggcat ctccacctcc
     3961 tcgcggtccg acctgggcat ccgaaggagg acgtcgtcca ctcggatggc taagggagag
     4021 ctcaaaaaaa aggatccggc tgctaacaaa gcccgaaagg aagctgagtt ggctgctgcc
     4081 accgctgagc aataactagc ataacccctt ggggcctcta aacgggtctt gaggggtttt
     4141 ttgctgaaag gaggaactat atccggatcg agatcctcta gagtcgacct gcaggcatgc
     4201 aagcttgtat tctatagtgt cacctaaatc gtatgtgtat gatacataag gttatgtatt
     4261 aattgtagcc gcgttctaac gacaatatgt acaagcctaa ttgtgtagca tctggcttac
     4321 tgaagcagac cctatcatct ctctcgtaaa ctgccgtcag agtcggtttg gttggacgaa
     4381 ccttctgagt ttctggtaac gccgttccgc accccggaaa tggtcagcga accaatcagc
     4441 agggtcatcg ctagccagat cctctacgcc ggacgcatcg tggccggcat caccggcgcc
     4501 acaggtgcgg ttgctggcgc ctatatcgcc gacatcaccg atggggaaga tcgggctcgc
     4561 cacttcgggc tcatgagcgc ttgtttcggc gtgggtatgg tggcaggccc cgtggccggg
     4621 ggactgttgg gcgccatctc cttgcaccat tccttgcggc ggcggtgctc aacggcctca
     4681 acctactact gggctgcttc ctaatgcagg agtcgcataa gggagagcgt cgatatggtg
     4741 cactctcagt acaatctgct ctgatgccgc atagttaagc cagccccgac acccgccaac
     4801 acccgctgac gcgccctgac gggcttgtct gctcccggca tccgcttaca gacaagctgt
     4861 gaccgtctcc gggagctgca tgtgtcagag gttttcaccg tcatcaccga aacgcgcgag
     4921 acgaaagggc ctcgtgatac gcctattttt ataggttaat gtcatgataa taatggtttc
     4981 ttagacgtca ggtggcactt ttcggggaaa tgtgcgcgga acccctattt gtttattttt
     5041 ctaaatacat tcaaatatgt atccgctcat gagacaataa ccctgataaa tgcttcaata
     5101 atattgaaaa aggaagagta tgagtattca acatttccgt gtcgccctta ttcccttttt
     5161 tgcggcattt tgccttcctg tttttgctca cccagaaacg ctggtgaaag taaaagatgc
     5221 tgaagatcag ttgggtgcac gagtgggtta catcgaactg gatctcaaca gcggtaagat
     5281 ccttgagagt tttcgccccg aagaacgttt tccaatgatg agcactttta aagttctgct
     5341 atgtggcgcg gtattatccc gtattgacgc cgggcaagag caactcggtc gccgcataca
     5401 ctattctcag aatgacttgg ttgagtactc accagtcaca gaaaagcatc ttacggatgg
     5461 catgacagta agagaattat gcagtgctgc cataaccatg agtgataaca ctgcggccaa
     5521 cttacttctg acaacgatcg gaggaccgaa ggagctaacc gcttttttgc acaacatggg
     5581 ggatcatgta actcgccttg atcgttggga accggagctg aatgaagcca taccaaacga
     5641 cgagcgtgac accacgatgc ctgtagcaat ggcaacaacg ttgcgcaaac tattaactgg
     5701 cgaactactt actctagctt cccggcaaca attaatagac tggatggagg cggataaagt
     5761 tgcaggacca cttctgcgct cggcccttcc ggctggctgg tttattgctg ataaatctgg
     5821 agccggtgag cgtgggtctc gcggtatcat tgcagcactg gggccagatg gtaagccctc
     5881 ccgtatcgta gttatctaca cgacggggag tcaggcaact atggatgaac gaaatagaca
     5941 gatcgctgag ataggtgcct cactgattaa gcattggtaa ctgtcagacc aagtttactc
     6001 atatatactt tagattgatt taaaacttca tttttaattt aaaaggatct aggtgaagat
     6061 cctttttgat aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc
     6121 agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg
     6181 ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct
     6241 accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa atactgtcct
     6301 tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct
     6361 cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg
     6421 gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc
     6481 gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga
     6541 gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg
     6601 cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta
     6661 tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg
     6721 ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg
     6781 ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtat
     6841 taccgccttt gagtgagctg ataccgctcg ccgcagccga acgaccgagc gcagcgagtc
     6901 agtgagcgag gaagcggaag agcgcccaat acgcaaaccg cctctccccg cgcgttggcc
     6961 gattcattaa tgcaggggga t
//
