LOCUS       sequence-39507-e        5370 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5370)
  AUTHORS   Kobayashi T, Antar AA, Boehme KW, Danthi P, Eby EA, Guglielmi KM, 
            Holm GH, Johnson EM, Maginnis MS, Naik S, Skelton WB, Wetzel JD, 
            Wilson GJ, Chappell JD, Dermody TS
  TITLE     A plasmid-based reverse genetics system for animal double-stranded 
            RNA viruses.
  JOURNAL   Cell Host Microbe. 2007 Apr 19;1(2):147-57.
  PUBMED    18005692
REFERENCE   2  (bases 1 to 5370)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell Host 
            Microbe."; date: "2007-04-19"; volume: "1(2)"; pages: "147-57"
COMMENT     Sequence Label: pT7-M1T3D
FEATURES             Location/Qualifiers
     source          1..5370
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             1121..1132
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(2471..2489)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      2485..2532
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     promoter        complement(2600..2618)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(2601..2618)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(2909..2928)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     primer_bind     complement(3139..3158)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     3258..3280
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(3318..3336)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        3404..3508
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(3509..3577,3578..4369)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(3727..3746)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      4540..5128
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5029..5048
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     5282..5299
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 gggtaatacg actcactata gctattcgcg gtcatggctt acatcgcagt tcctgcggtg
       61 gtggattcac gttcgagtga ggctattgga ctgctagaat cgtttggagt agacgctggg
      121 gctgacgcga atgacgtttc atatcaagat catgactatg tgttggatca gttacagtac
      181 atgttagatg gatatgaggc tggtgacgtt atcgatgcac tcgtccacaa gaattggtta
      241 catcactctg tctattgctt gttgccaccc aaaagtcaac tattagagta ttggaaaagt
      301 aatccttcag cgataccgga caacgttgat cgtcggcttc gtaaacgact aatgctaaag
      361 aaagatctca ggaaagatga tgaatacaat cagctagcgc gtgctttcaa gatatcggat
      421 gtctacgcac ctctcatctc atccacgacg tcaccgatga caatgataca gaacttgaat
      481 caaggcgaga tcgtgtacac cacgacggac agggtaatag gggctagaat cttgttatat
      541 gctcctagaa agtactatgc gtcaactctg tcatttacta tgactaagtg catcattccg
      601 tttggtaaag aggtgggtcg tgttcctcac tctcgattta atgttggcac attttcgtca
      661 attgctaccc cgaaatgttt tgtcatgagt ggggttgata ttgagtccat cccaaatgaa
      721 tttatcaagt tgttttacca gcgcgtcaag agtgttcacg ctaacatact aaatgacata
      781 tctcctcaga tcgtctctga catgataaac agaaagcgtc tgcgcgttca tactccatca
      841 gatcgtcgag ccgcgcagtt gatgcatttg ccttaccatg ttaaacgagg agcgtctcac
      901 gtcgacgttt acaaggtgga tgttgtagac atgttgttcg aggtagtgga tgtggccgat
      961 gggttgcgca acgtatctag gaaactaact atgcataccg ttccggtatg tattcttgaa
     1021 atgttgggta ttgagattgc ggactattgc attcgtcgag aggatggaat gctcacagat
     1081 tggttcctac ttttaaccat gctatctgat ggcttgactg atagaaggac gcattgtcaa
     1141 tacttgatta atccgtcaag tgtgcctcct gatgtgatac ttaacatctc aattactgga
     1201 tttataaata gacatacaat cgatgtcatg cctgacatat atgactttgt taaacccatt
     1261 ggcgctgtgc tgcctaaggg atcatttaaa tcaacaatta tgagagttct tgattcaata
     1321 tcaatattag gaatccaaat catgccgcgc gcgcatgtag ttgactcaga tgaggtgggc
     1381 gagcaaatgg agcctacgtt tgagcaggcg gttatggaga tatacaaagg gattgctggc
     1441 gttgactcgc tggatgatct catcaagtgg gtgctgaact cggatctcat tccgcatgat
     1501 gacaggcttg gtcaattatt tcaagcgttt ttgcctctcg caaaggactt attagctcca
     1561 atggccagaa agttttatga taactcaatg agtgagggta gattgctaac attcgctcat
     1621 gccgacagtg agttgctgaa cgcaaattat tttggtcatt tattgcgact aaaaatacca
     1681 tatattacag aggttaatct gatgattcgc aagaatcgtg agggtggaga gctatttcag
     1741 cttgtgttat cttatctata taaaatgtat gctactagcg cgcagcctaa atggtttgga
     1801 tcattattgc gattgttaat atgtccctgg ttacatatgg agaaattaat aggagaagca
     1861 gacccggcat ctacgtcggc tgaaattggg tggcatatcc ctcgtgaaca gctgatgcaa
     1921 gatggatggt gtggatgtga agacggattc attccctatg ttagcatacg tgcgccaaga
     1981 ctggttatag aggagttgat ggagaagaac tggggccaat atcatgccca agttattgtc
     2041 actgatcagc ttgtcgtagg cgaaccgcgg agggtatctg ctaaggctgt gatcaagggt
     2101 aaccacttac cagttaagtt agtttcacga tttgcatgtt tcacattgac ggcgaagtat
     2161 gagatgaggc tttcgtgcgg ccatagcact ggacgtggag ctgcatacag tgcgagacta
     2221 gctttccgat ctgacttggc gtgatccgtg acatgcgtag tgtgacacct gctcctaggt
     2281 caatgggggt agggggcggg ctaagactac gtacgcgctt catcgggtcg gcatggcatc
     2341 tccacctcct cgcggtccga cctgggcatc cgaaggagga cgtcgtccac tcggatggct
     2401 aagggagagc tcaaaaaaaa ggatccggct gctaacaaag cccgaaagga agctgagttg
     2461 gctgctgcca ccgctgagca ataactagca taaccccttg gggcctctaa acgggtcttg
     2521 aggggttttt tgctgaaagg aggaactata tccggatcga gatcctctag agtcgacctg
     2581 caggcatgca agcttgtatt ctatagtgtc acctaaatcg tatgtgtatg atacataagg
     2641 ttatgtatta attgtagccg cgttctaacg acaatatgta caagcctaat tgtgtagcat
     2701 ctggcttact gaagcagacc ctatcatctc tctcgtaaac tgccgtcaga gtcggtttgg
     2761 ttggacgaac cttctgagtt tctggtaacg ccgttccgca ccccggaaat ggtcagcgaa
     2821 ccaatcagca gggtcatcgc tagccagatc ctctacgccg gacgcatcgt ggccggcatc
     2881 accggcgcca caggtgcggt tgctggcgcc tatatcgccg acatcaccga tggggaagat
     2941 cgggctcgcc acttcgggct catgagcgct tgtttcggcg tgggtatggt ggcaggcccc
     3001 gtggccgggg gactgttggg cgccatctcc ttgcaccatt ccttgcggcg gcggtgctca
     3061 acggcctcaa cctactactg ggctgcttcc taatgcagga gtcgcataag ggagagcgtc
     3121 gatatggtgc actctcagta caatctgctc tgatgccgca tagttaagcc agccccgaca
     3181 cccgccaaca cccgctgacg cgccctgacg ggcttgtctg ctcccggcat ccgcttacag
     3241 acaagctgtg accgtctccg ggagctgcat gtgtcagagg ttttcaccgt catcaccgaa
     3301 acgcgcgaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat
     3361 aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg
     3421 tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat
     3481 gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat
     3541 tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt
     3601 aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag
     3661 cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa
     3721 agttctgcta tgtggcgcgg tattatcccg tattgacgcc gggcaagagc aactcggtcg
     3781 ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct
     3841 tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac
     3901 tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca
     3961 caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat
     4021 accaaacgac gagcgtgaca ccacgatgcc tgtagcaatg gcaacaacgt tgcgcaaact
     4081 attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc
     4141 ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga
     4201 taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg
     4261 taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg
     4321 aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca
     4381 agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta
     4441 ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca
     4501 ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg
     4561 cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga
     4621 tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa
     4681 tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc
     4741 tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg
     4801 tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac
     4861 ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct
     4921 acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc
     4981 ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg
     5041 gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg
     5101 ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct
     5161 ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga
     5221 taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg
     5281 cagcgagtca gtgagcgagg aagcggaaga gcgcccaata cgcaaaccgc ctctccccgc
     5341 gcgttggccg attcattaat gcagggggat
//
