LOCUS       sequence-39508-e        5269 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5269)
  AUTHORS   Kobayashi T, Antar AA, Boehme KW, Danthi P, Eby EA, Guglielmi KM, 
            Holm GH, Johnson EM, Maginnis MS, Naik S, Skelton WB, Wetzel JD, 
            Wilson GJ, Chappell JD, Dermody TS
  TITLE     A plasmid-based reverse genetics system for animal double-stranded 
            RNA viruses.
  JOURNAL   Cell Host Microbe. 2007 Apr 19;1(2):147-57.
  PUBMED    18005692
REFERENCE   2  (bases 1 to 5269)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell Host 
            Microbe."; date: "2007-04-19"; volume: "1(2)"; pages: "147-57"
COMMENT     Sequence Label: pT7-M2T3D
FEATURES             Location/Qualifiers
     source          1..5269
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(2370..2388)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      2384..2431
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     promoter        complement(2499..2517)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(2500..2517)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(2808..2827)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     primer_bind     complement(3038..3057)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     3157..3179
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(3217..3235)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        3303..3407
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(3408..3476,3477..4268)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(3626..3645)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      4439..5027
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4928..4947
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     5181..5198
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 agataatacg actcactata gctaatctgc tgaccgttac tctgcaaaga tggggaacgc
       61 ttcctctatc gttcagacga tcaacgtcac tggagatggc aatgtattta aaccatcagc
      121 tgaaacttca tctaccgctg taccatcgtt aagcttatca cctggaatgc tgaatcccgg
      181 aggggtacca tggattgctg ttggagatga gacatctgtg acttcaccag gcgcattacg
      241 tcgaatgacg tcaaaggaca tcccggaaac ggcaataatc aacacagaca attcatcagg
      301 cgccgtgcca agcgaatcag ccttggtgcc ctacatcgat gagccgctgg tagtggttac
      361 agagcatgct attaccaact tcaccaaagc tgagatggca cttgaattca atcgtgagtt
      421 ccttgacaag atgcgtgtgc tgtcagtgtc accaaaatat tcggatcttc tgacctatgt
      481 tgactgctac gtcggtgtgt ctgctcgtca ggctttaaac aattttcaga aacaagtgcc
      541 tgtgattaca cctactaggc agacgatgta tgtcgactcg atacaagcgg ccttgaaagc
      601 tttagaaaag tgggagattg atctgagagt ggctcaaacg ttgctgccta cgaacgttcc
      661 gattggagaa gtctcttgtc caatgcagtc ggtagtgaaa ctgctggatg atcagctgcc
      721 agatgacagc ctgatacgga ggtatcccaa ggaagccgcc gtcgctttgg ctaaacgaaa
      781 cgggggaata caatggatgg acgtatcaga aggcaccgtg atgaacgagg ctgtcaacgc
      841 tgttgcagct agtgcactgg caccttcagc atcagcccca cccttagaag agaagtcaaa
      901 gttaaccgaa caagcgatgg atctcgtgac cgcggctgag cctgagataa ttgcctcact
      961 cgcgccagtt cccgcacccg tgtttgccat accacctaaa ccagcagatt ataatgtgcg
     1021 tactctgagg atcgacgagg ccacttggct gcgaatgatt ccaaaatcaa tgaacacacc
     1081 ttttcaaatc caggtgactg ataacacagg aactaattgg catctcaatt tgaggggggg
     1141 gactcgtgta gtgaatctgg accaaatcgc tccgatgcgg tttgtattag atttaggggg
     1201 aaagagttat aaagagacga gctgggatcc aaacggcaag aaggtcggat tcatcgtttt
     1261 tcaatcgaag ataccattcg aactttggac tgctgcttca cagatcggtc aagccacggt
     1321 ggttaactat gtccaactat acgctgaaga cagctcattt accgcgcagt ctatcattgc
     1381 tactacctct ttggcttata actatgagcc tgagcagttg aataagactg accctgagat
     1441 gaattattat cttttggcga cctttataga ctcagccgct ataacgccaa cgaatatgac
     1501 acagcctgat gtttgggatg ccttgctgac gatgtcccca ctatcagctg gcgaggtgac
     1561 agtgaagggt gcggtagtga gtgaagtagt ccctgcagac ttgataggta gctacactcc
     1621 agaatcccta aacgcctcac ttccgaatga tgctgctaga tgcatgatcg atagagcttc
     1681 gaagatagcc gaagcaatca agattgatga tgatgctgga ccagatgaat attccccaaa
     1741 ctctgtacca attcaaggtc agcttgctat ctcgcaactc gaaactggat atggtgtgcg
     1801 aatattcaac cctaaaggga tcctttctaa aattgcatct agggcaatgc aggctttcat
     1861 tggtgacccg agcacaatca tcacgcaggc ggcgccagtg ttatcagaca agaataattg
     1921 gattgcattg gcacagggag tgaaaactag tctgcgtact aaaagtctat cagcgggagt
     1981 gaagactgca gtgagtaagc tgagctcatc tgagtctatc cagaattgga ctcaaggatt
     2041 cttggataaa gtgtcagcgc attttccagc accaaagccc gattgtccga ctagcggaga
     2101 tagtggtgaa tcgtctaatc gccgagtgaa gcgcgactca tacgcaggag tggtcaaacg
     2161 tgggtacaca cgttaggccg ctcgccctgg tgacgcgggg ttaagggatg caggcaaatc
     2221 atcgggtcgg catggcatct ccacctcctc gcggtccgac ctgggcatcc gaaggaggac
     2281 gtcgtccact cggatggcta agggagagct caaaaaaaag gatccggctg ctaacaaagc
     2341 ccgaaaggaa gctgagttgg ctgctgccac cgctgagcaa taactagcat aaccccttgg
     2401 ggcctctaaa cgggtcttga ggggtttttt gctgaaagga ggaactatat ccggatcgag
     2461 atcctctaga gtcgacctgc aggcatgcaa gcttgtattc tatagtgtca cctaaatcgt
     2521 atgtgtatga tacataaggt tatgtattaa ttgtagccgc gttctaacga caatatgtac
     2581 aagcctaatt gtgtagcatc tggcttactg aagcagaccc tatcatctct ctcgtaaact
     2641 gccgtcagag tcggtttggt tggacgaacc ttctgagttt ctggtaacgc cgttccgcac
     2701 cccggaaatg gtcagcgaac caatcagcag ggtcatcgct agccagatcc tctacgccgg
     2761 acgcatcgtg gccggcatca ccggcgccac aggtgcggtt gctggcgcct atatcgccga
     2821 catcaccgat ggggaagatc gggctcgcca cttcgggctc atgagcgctt gtttcggcgt
     2881 gggtatggtg gcaggccccg tggccggggg actgttgggc gccatctcct tgcaccattc
     2941 cttgcggcgg cggtgctcaa cggcctcaac ctactactgg gctgcttcct aatgcaggag
     3001 tcgcataagg gagagcgtcg atatggtgca ctctcagtac aatctgctct gatgccgcat
     3061 agttaagcca gccccgacac ccgccaacac ccgctgacgc gccctgacgg gcttgtctgc
     3121 tcccggcatc cgcttacaga caagctgtga ccgtctccgg gagctgcatg tgtcagaggt
     3181 tttcaccgtc atcaccgaaa cgcgcgagac gaaagggcct cgtgatacgc ctatttttat
     3241 aggttaatgt catgataata atggtttctt agacgtcagg tggcactttt cggggaaatg
     3301 tgcgcggaac ccctatttgt ttatttttct aaatacattc aaatatgtat ccgctcatga
     3361 gacaataacc ctgataaatg cttcaataat attgaaaaag gaagagtatg agtattcaac
     3421 atttccgtgt cgcccttatt cccttttttg cggcattttg ccttcctgtt tttgctcacc
     3481 cagaaacgct ggtgaaagta aaagatgctg aagatcagtt gggtgcacga gtgggttaca
     3541 tcgaactgga tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc
     3601 caatgatgag cacttttaaa gttctgctat gtggcgcggt attatcccgt attgacgccg
     3661 ggcaagagca actcggtcgc cgcatacact attctcagaa tgacttggtt gagtactcac
     3721 cagtcacaga aaagcatctt acggatggca tgacagtaag agaattatgc agtgctgcca
     3781 taaccatgag tgataacact gcggccaact tacttctgac aacgatcgga ggaccgaagg
     3841 agctaaccgc ttttttgcac aacatggggg atcatgtaac tcgccttgat cgttgggaac
     3901 cggagctgaa tgaagccata ccaaacgacg agcgtgacac cacgatgcct gtagcaatgg
     3961 caacaacgtt gcgcaaacta ttaactggcg aactacttac tctagcttcc cggcaacaat
     4021 taatagactg gatggaggcg gataaagttg caggaccact tctgcgctcg gcccttccgg
     4081 ctggctggtt tattgctgat aaatctggag ccggtgagcg tgggtctcgc ggtatcattg
     4141 cagcactggg gccagatggt aagccctccc gtatcgtagt tatctacacg acggggagtc
     4201 aggcaactat ggatgaacga aatagacaga tcgctgagat aggtgcctca ctgattaagc
     4261 attggtaact gtcagaccaa gtttactcat atatacttta gattgattta aaacttcatt
     4321 tttaatttaa aaggatctag gtgaagatcc tttttgataa tctcatgacc aaaatccctt
     4381 aacgtgagtt ttcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt
     4441 gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag
     4501 cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta actggcttca
     4561 gcagagcgca gataccaaat actgtccttc tagtgtagcc gtagttaggc caccacttca
     4621 agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg
     4681 ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg
     4741 cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct
     4801 acaccgaact gagataccta cagcgtgagc tatgagaaag cgccacgctt cccgaaggga
     4861 gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc
     4921 ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg
     4981 agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg
     5041 cggccttttt acggttcctg gccttttgct ggccttttgc tcacatgttc tttcctgcgt
     5101 tatcccctga ttctgtggat aaccgtatta ccgcctttga gtgagctgat accgctcgcc
     5161 gcagccgaac gaccgagcgc agcgagtcag tgagcgagga agcggaagag cgcccaatac
     5221 gcaaaccgcc tctccccgcg cgttggccga ttcattaatg cagggggat
//
