LOCUS       sequence-39509-e        5528 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5528)
  AUTHORS   Kobayashi T, Antar AA, Boehme KW, Danthi P, Eby EA, Guglielmi KM, 
            Holm GH, Johnson EM, Maginnis MS, Naik S, Skelton WB, Wetzel JD, 
            Wilson GJ, Chappell JD, Dermody TS
  TITLE     A plasmid-based reverse genetics system for animal double-stranded 
            RNA viruses.
  JOURNAL   Cell Host Microbe. 2007 Apr 19;1(2):147-57.
  PUBMED    18005692
REFERENCE   2  (bases 1 to 5528)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell Host 
            Microbe."; date: "2007-04-19"; volume: "1(2)"; pages: "147-57"
COMMENT     Sequence Label: pT7-M3T3D
FEATURES             Location/Qualifiers
     source          1..5528
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(2629..2647)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      2643..2690
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     promoter        complement(2758..2776)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(2759..2776)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(3067..3086)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     primer_bind     complement(3297..3316)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     3416..3438
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(3476..3494)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        3562..3666
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(3667..3735,3736..4527)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(3885..3904)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      4698..5286
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5187..5206
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     5440..5457
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 atcgatcccc taggttgttc tctatctttg tgatgtggct ctgaaacaaa ccaggtgtga
       61 ttacagtaac aatttcaatt taaattccga tataaatttc aatgagagga aaattattaa
      121 tcttcctcat agttattcgc acacaaaaga tcctaaaaat tcttctttct ttttgtgtgt
      181 ccggggtcgg catggcatct ccacctcctc gcggtccgac cggttaatac gactcactat
      241 agctaaagtg accgtggtca tggcttcatt caagggattc tccgccaaca ctgttccagt
      301 ttctaaggcc aagcgtgaca tatcatctct tgccgctact cctggacttc gttcacaatc
      361 cttcactccg tctgtggata tgtctcaatc gcgtgaattc ctcacaaagg caattgagca
      421 agggtccatg tctatacctt atcagcatgt gaatgtaccg aaagttgatc gtaaagttgt
      481 tagcctggta gtgcgacctt tctcttcagg tgctttctct atctctggag tgatttcgcc
      541 agcccatgcc tatctactag agtgtctacc ccagcttgag caggcgatgg cttttgttgc
      601 ttcacctgag tctttccagg cttccgacgt cgcgaagcgc tttgccataa agccaggtat
      661 gagcctccag gatgccatca ctgcctttat taactttgtg tccgcgatgc tgaaaatgac
      721 ggtgactcgt caaaactttg acgttattgt ggctgagatc gagaggcttg cttcaaccag
      781 cgtgtccgtc aggactaaag aagcgaaggt tgctgatgag gagctaatgc tattcgggtt
      841 agatcataga gggccacagc agctggatgt ttctgacgct aaagggataa tgaaggctgc
      901 tgatattcag acaactcatg atgtccattt ggcaccaggc gttggtaata ttgatcctga
      961 aatctataac gaggggcggt tcatgttcat gcagcacaag ccacttgcgg cggatcaatc
     1021 gtatttcacc ttggagactg cggattattt caagatttat ccaacatacg atgaacatga
     1081 tggcaggatg gctgaccaaa agcagtcggg attgatactg tgtactaagg acgaggtatt
     1141 ggctgagcaa actatattta aactggacgc ccctgatgac aagactgttc atctgttgga
     1201 tcgcgatgac gaccacgttg ttgccagatt tactaaggta tttatagagg acgtggctcc
     1261 cgggcatcat gctgctcaaa gatcgggaca acgctctgtg cttgatgacc tatatgcgaa
     1321 tacgcaagtg atttccatta cttctgctgc tttaaagtgg gtggtcaagc acggcgtatc
     1381 tgatggaatc gtgaacagga agaatgtcaa agtgtgtgtt ggttttgacc ccctgtacac
     1441 cttgtctaca cataacgggg tgtccttatg tgccctgctg atggacgaaa aactctctgt
     1501 gctgaacagt gcgtgtcgta tgacgttacg ctcactcatg aagaccggac gcgacgttga
     1561 tgcacacaga gcttttcagc gagtcctctc tcaaggatac acatcgctaa tgtgctacta
     1621 tcatccttca cggaagttgg catatggtga ggtgctcttt ctagaacgat ccaatgacgt
     1681 gacagatggg atcaagcttc agttggacgc atctagacag tgtcatgaat gtcctgtgtt
     1741 gcagcagaaa gtggttgagt tagagaaaca gattattatg cagaagtcaa tccagtcaga
     1801 ccctacccca gtggcgctgc aaccattgtt gtctcagttg cgtgagttgt ctagtgaagt
     1861 tactaggcta cagatggagt tgagtcgagc tcagtccctg aatgctcagt tggaggcgga
     1921 tgtcaagtca gctcaatcat gtagcttgga tatgtatctg agacaccaca cttgcattaa
     1981 tggtcatgct aaagaagatg aattgcttga cgctgtgcgt gtcgcgccgg atgtgaggag
     2041 agaaatcatg gaaaagagga gtgaagtgag acaaggttgg tgcgaacgta tttctaagga
     2101 agcagctgcc aaatgtcaaa ctgttattga tgacctgact ttgatgaatg gaaagcaagc
     2161 acaagagata acagaattac gtgattcggc tgaaaaatat gagaaacaga ttgcagagct
     2221 ggtgagtacc atcacccaaa accagataac gtatcagcaa gagctacaag ccttggtagc
     2281 gaaaaatgtg gaattggacg cgttgaatca gcgtcaggct aagtctttgc gtattactcc
     2341 ctctcttcta tcagccactc ctatcgattc agctgatggt gttgctgact taattgattt
     2401 ctctgttcca actgatgagt tgtaaataat ccgtgatgca gtgttgccct aatcccttaa
     2461 gccttcccga cccccattca tcgggtcggc atggcatctc cacctcctcg cggtccgacc
     2521 tgggcatccg aaggaggacg tcgtccactc ggatggctaa gggagagctc aaaaaaaagg
     2581 atccggctgc taacaaagcc cgaaaggaag ctgagttggc tgctgccacc gctgagcaat
     2641 aactagcata accccttggg gcctctaaac gggtcttgag gggttttttg ctgaaaggag
     2701 gaactatatc cggatcgaga tcctctagag tcgacctgca ggcatgcaag cttgtattct
     2761 atagtgtcac ctaaatcgta tgtgtatgat acataaggtt atgtattaat tgtagccgcg
     2821 ttctaacgac aatatgtaca agcctaattg tgtagcatct ggcttactga agcagaccct
     2881 atcatctctc tcgtaaactg ccgtcagagt cggtttggtt ggacgaacct tctgagtttc
     2941 tggtaacgcc gttccgcacc ccggaaatgg tcagcgaacc aatcagcagg gtcatcgcta
     3001 gccagatcct ctacgccgga cgcatcgtgg ccggcatcac cggcgccaca ggtgcggttg
     3061 ctggcgccta tatcgccgac atcaccgatg gggaagatcg ggctcgccac ttcgggctca
     3121 tgagcgcttg tttcggcgtg ggtatggtgg caggccccgt ggccggggga ctgttgggcg
     3181 ccatctcctt gcaccattcc ttgcggcggc ggtgctcaac ggcctcaacc tactactggg
     3241 ctgcttccta atgcaggagt cgcataaggg agagcgtcga tatggtgcac tctcagtaca
     3301 atctgctctg atgccgcata gttaagccag ccccgacacc cgccaacacc cgctgacgcg
     3361 ccctgacggg cttgtctgct cccggcatcc gcttacagac aagctgtgac cgtctccggg
     3421 agctgcatgt gtcagaggtt ttcaccgtca tcaccgaaac gcgcgagacg aaagggcctc
     3481 gtgatacgcc tatttttata ggttaatgtc atgataataa tggtttctta gacgtcaggt
     3541 ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt tatttttcta aatacattca
     3601 aatatgtatc cgctcatgag acaataaccc tgataaatgc ttcaataata ttgaaaaagg
     3661 aagagtatga gtattcaaca tttccgtgtc gcccttattc ccttttttgc ggcattttgc
     3721 cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga agatcagttg
     3781 ggtgcacgag tgggttacat cgaactggat ctcaacagcg gtaagatcct tgagagtttt
     3841 cgccccgaag aacgttttcc aatgatgagc acttttaaag ttctgctatg tggcgcggta
     3901 ttatcccgta ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat
     3961 gacttggttg agtactcacc agtcacagaa aagcatctta cggatggcat gacagtaaga
     4021 gaattatgca gtgctgccat aaccatgagt gataacactg cggccaactt acttctgaca
     4081 acgatcggag gaccgaagga gctaaccgct tttttgcaca acatggggga tcatgtaact
     4141 cgccttgatc gttgggaacc ggagctgaat gaagccatac caaacgacga gcgtgacacc
     4201 acgatgcctg tagcaatggc aacaacgttg cgcaaactat taactggcga actacttact
     4261 ctagcttccc ggcaacaatt aatagactgg atggaggcgg ataaagttgc aggaccactt
     4321 ctgcgctcgg cccttccggc tggctggttt attgctgata aatctggagc cggtgagcgt
     4381 gggtctcgcg gtatcattgc agcactgggg ccagatggta agccctcccg tatcgtagtt
     4441 atctacacga cggggagtca ggcaactatg gatgaacgaa atagacagat cgctgagata
     4501 ggtgcctcac tgattaagca ttggtaactg tcagaccaag tttactcata tatactttag
     4561 attgatttaa aacttcattt ttaatttaaa aggatctagg tgaagatcct ttttgataat
     4621 ctcatgacca aaatccctta acgtgagttt tcgttccact gagcgtcaga ccccgtagaa
     4681 aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca
     4741 aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt
     4801 ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgtccttct agtgtagccg
     4861 tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc
     4921 ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga
     4981 cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc
     5041 agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc
     5101 gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca
     5161 ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg
     5221 tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta
     5281 tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct
     5341 cacatgttct ttcctgcgtt atcccctgat tctgtggata accgtattac cgcctttgag
     5401 tgagctgata ccgctcgccg cagccgaacg accgagcgca gcgagtcagt gagcgaggaa
     5461 gcggaagagc gcccaatacg caaaccgcct ctccccgcgc gttggccgat tcattaatgc
     5521 agggggat
//
