LOCUS       sequence-39510-e        4587 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4587)
  AUTHORS   Kobayashi T, Antar AA, Boehme KW, Danthi P, Eby EA, Guglielmi KM, 
            Holm GH, Johnson EM, Maginnis MS, Naik S, Skelton WB, Wetzel JD, 
            Wilson GJ, Chappell JD, Dermody TS
  TITLE     A plasmid-based reverse genetics system for animal double-stranded 
            RNA viruses.
  JOURNAL   Cell Host Microbe. 2007 Apr 19;1(2):147-57.
  PUBMED    18005692
REFERENCE   2  (bases 1 to 4587)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell Host 
            Microbe."; date: "2007-04-19"; volume: "1(2)"; pages: "147-57"
COMMENT     Sequence Label: pT7-S2T3D
FEATURES             Location/Qualifiers
     source          1..4587
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(1688..1706)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      1702..1749
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     promoter        complement(1817..1835)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(1818..1835)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(2126..2145)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     primer_bind     complement(2356..2375)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     2475..2497
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(2535..2553)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        2621..2725
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(2726..2794,2795..3586)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(2944..2963)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      3757..4345
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4246..4265
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     4499..4516
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 ctaggttgtt ctctatcttt gtgatgtggc tctgaaacaa accaggtgtg attacagtaa
       61 caatttcaat ttaaattccg atataaattt caatgagagg aaaattatta atcttcctca
      121 tagttattcg cacacaaaag atcctaaaaa ttcttctttc tttttgtgtg tccggggtcg
      181 gcatggctct agataatacg actcactata gctattcgct ggtcagttat ggctcgcgct
      241 gcgttcctat tcaagactgt tgggtttggt ggtctgcaaa atgtgccaat taacgacgaa
      301 ctatcttcac atctactccg agctggtaat tcaccatggc agttaacaca gtttttagac
      361 tggataagcc ttgggagggg tttagctaca tcggctctcg ttccgacggc tgggtcaaga
      421 tactatcaaa tgagttgcct tctaagtggc actctccaga ttccgttccg tcctaaccac
      481 cgatggggag acattaggtt cttacgctta gtgtggtcag ctcctactct cgatggatta
      541 gtcgtagctc caccacaagt tttggctcag cccgctttgc aagcacaggc agatcgagtg
      601 tacgactgcg atgattatcc atttctagcg cgtgatccaa gattcaaaca tcgggtgtat
      661 cagcaattga gtgctgtaac tctacttaac ttgacaggtt ttggcccgat ttcctacgtt
      721 cgagtggatg aagatatgtg gagtggagat gtgaaccagc ttctcatgaa ctatttcggg
      781 cacacgtttg cagagattgc atacacattg tgtcaagcct cggctaatag gccttgggaa
      841 tatgacggta catatgctag gatgactcag attgtgttat ccttgttctg gctatcgtat
      901 gtcggtgtaa tccatcagca gaatacgtat cggacattct attttcagtg taatcggcga
      961 ggtgacgccg ctgaggtgtg gattctttct tgttcgttga accattccgc acaaattaga
     1021 ccgggtaatc gtagcttatt cgttatgcca actagcccag attggaacat ggacgtcaat
     1081 ttgatcctga gttcaacgtt gacggggtgt ttgtgttcgg gttcacagct gccactgatt
     1141 gacaataatt cagtacctgc agtgtcgcgt aacatccatg gctggactgg tagagctggt
     1201 aaccaattgc atgggttcca ggtgagacga atggtgactg aattttgtga caggttgaga
     1261 cgcgatggtg tcatgaccca agctcagcag aatcaagttg aagcgttggc agatcagact
     1321 caacagttta agagggacaa gctcgaaacg tgggcgagag aagacgatca atataatcag
     1381 gctcatccca actccacaat gttccgtacg aaaccattta cgaatgcgca atggggacga
     1441 ggtaatacgg gggcgactag tgccgcgatt gcagccctta tctgatcgtc ttggagtgag
     1501 ggggtccccc cacacccctc acgactgacc acacattcat cgggtcggca tggcatctcc
     1561 acctcctcgc ggtccgacct gggcatccga aggaggacgt cgtccactcg gatggctaag
     1621 ggagagctca aaaaaaagga tccggctgct aacaaagccc gaaaggaagc tgagttggct
     1681 gctgccaccg ctgagcaata actagcataa ccccttgggg cctctaaacg ggtcttgagg
     1741 ggttttttgc tgaaaggagg aactatatcc ggatcgagat cctctagagt cgacctgcag
     1801 gcatgcaagc ttgtattcta tagtgtcacc taaatcgtat gtgtatgata cataaggtta
     1861 tgtattaatt gtagccgcgt tctaacgaca atatgtacaa gcctaattgt gtagcatctg
     1921 gcttactgaa gcagacccta tcatctctct cgtaaactgc cgtcagagtc ggtttggttg
     1981 gacgaacctt ctgagtttct ggtaacgccg ttccgcaccc cggaaatggt cagcgaacca
     2041 atcagcaggg tcatcgctag ccagatcctc tacgccggac gcatcgtggc cggcatcacc
     2101 ggcgccacag gtgcggttgc tggcgcctat atcgccgaca tcaccgatgg ggaagatcgg
     2161 gctcgccact tcgggctcat gagcgcttgt ttcggcgtgg gtatggtggc aggccccgtg
     2221 gccgggggac tgttgggcgc catctccttg caccattcct tgcggcggcg gtgctcaacg
     2281 gcctcaacct actactgggc tgcttcctaa tgcaggagtc gcataaggga gagcgtcgat
     2341 atggtgcact ctcagtacaa tctgctctga tgccgcatag ttaagccagc cccgacaccc
     2401 gccaacaccc gctgacgcgc cctgacgggc ttgtctgctc ccggcatccg cttacagaca
     2461 agctgtgacc gtctccggga gctgcatgtg tcagaggttt tcaccgtcat caccgaaacg
     2521 cgcgagacga aagggcctcg tgatacgcct atttttatag gttaatgtca tgataataat
     2581 ggtttcttag acgtcaggtg gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt
     2641 atttttctaa atacattcaa atatgtatcc gctcatgaga caataaccct gataaatgct
     2701 tcaataatat tgaaaaagga agagtatgag tattcaacat ttccgtgtcg cccttattcc
     2761 cttttttgcg gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa
     2821 agatgctgaa gatcagttgg gtgcacgagt gggttacatc gaactggatc tcaacagcgg
     2881 taagatcctt gagagttttc gccccgaaga acgttttcca atgatgagca cttttaaagt
     2941 tctgctatgt ggcgcggtat tatcccgtat tgacgccggg caagagcaac tcggtcgccg
     3001 catacactat tctcagaatg acttggttga gtactcacca gtcacagaaa agcatcttac
     3061 ggatggcatg acagtaagag aattatgcag tgctgccata accatgagtg ataacactgc
     3121 ggccaactta cttctgacaa cgatcggagg accgaaggag ctaaccgctt ttttgcacaa
     3181 catgggggat catgtaactc gccttgatcg ttgggaaccg gagctgaatg aagccatacc
     3241 aaacgacgag cgtgacacca cgatgcctgt agcaatggca acaacgttgc gcaaactatt
     3301 aactggcgaa ctacttactc tagcttcccg gcaacaatta atagactgga tggaggcgga
     3361 taaagttgca ggaccacttc tgcgctcggc ccttccggct ggctggttta ttgctgataa
     3421 atctggagcc ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc cagatggtaa
     3481 gccctcccgt atcgtagtta tctacacgac ggggagtcag gcaactatgg atgaacgaaa
     3541 tagacagatc gctgagatag gtgcctcact gattaagcat tggtaactgt cagaccaagt
     3601 ttactcatat atactttaga ttgatttaaa acttcatttt taatttaaaa ggatctaggt
     3661 gaagatcctt tttgataatc tcatgaccaa aatcccttaa cgtgagtttt cgttccactg
     3721 agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt
     3781 aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca
     3841 agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac
     3901 tgtccttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac
     3961 atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct
     4021 taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg
     4081 gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga gatacctaca
     4141 gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt
     4201 aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta
     4261 tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc
     4321 gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc
     4381 cttttgctgg ccttttgctc acatgttctt tcctgcgtta tcccctgatt ctgtggataa
     4441 ccgtattacc gcctttgagt gagctgatac cgctcgccgc agccgaacga ccgagcgcag
     4501 cgagtcagtg agcgaggaag cggaagagcg cccaatacgc aaaccgcctc tccccgcgcg
     4561 ttggccgatt cattaatgca gggggat
//
