LOCUS       sequence-39511-e        4361 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4361)
  AUTHORS   Kobayashi T, Antar AA, Boehme KW, Danthi P, Eby EA, Guglielmi KM, 
            Holm GH, Johnson EM, Maginnis MS, Naik S, Skelton WB, Wetzel JD, 
            Wilson GJ, Chappell JD, Dermody TS
  TITLE     A plasmid-based reverse genetics system for animal double-stranded 
            RNA viruses.
  JOURNAL   Cell Host Microbe. 2007 Apr 19;1(2):147-57.
  PUBMED    18005692
REFERENCE   2  (bases 1 to 4361)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell Host 
            Microbe."; date: "2007-04-19"; volume: "1(2)"; pages: "147-57"
COMMENT     Sequence Label: pT7-S3T3D
FEATURES             Location/Qualifiers
     source          1..4361
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(1462..1480)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      1476..1523
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     promoter        complement(1591..1609)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(1592..1609)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(1900..1919)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     primer_bind     complement(2130..2149)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     2249..2271
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(2309..2327)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        2395..2499
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(2500..2568,2569..3360)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(2718..2737)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      3531..4119
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4020..4039
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     4273..4290
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 ggaaacagct atgaccatga ttacgccaag cttggtaccg agctcggatc cactagtaac
       61 ggccgccagt gtgctggaat tcggcttctc gcggtccggt taatacgact cactatagct
      121 aaagtcacgc ctgtcgtcgt cactatggct tcctcactca gagctgcgat ctccaagatc
      181 aagagggatg acgtcggtca gcaagtttgt cctaattatg tcatgctgcg gtcctctgtc
      241 acaacaaagg tggtacgaaa tgtggttgag tatcaaattc gtacgggcgg attcttttcg
      301 tgcttagcta tgctaaggcc actccagtac gctaagcgtg agcgtttgct tggtcagagg
      361 aatctggaac gtatatcgac tagggatatc cttcagactc gtgatttaca ctcactatgt
      421 atgccaactc ctgatgcgcc aatgtctaat catcaagcat ccaccatgag agagctgatt
      481 tgcagttact tcaaggtcga tcatgcggat gggttgaaat atatacccat ggatgagaga
      541 tactctccgt catcacttgc cagattgttt accatgggca tggctgggct gcacattacc
      601 actgagccat cttataagcg tgttccgatt atgcacttag ctgcggactt ggactgtatg
      661 acgctggctc taccttacat gattacgctt gatggtgata ctgtggttcc tgtcgctcca
      721 acactgtcag cggaacagct tctggacgac ggactcaaag gattagcatg catggatatc
      781 tcctatggat gtgaggtgga cgcgaatagc cggccggctg gtgatcagag tatggactct
      841 tcacgctgca tcaacgagtt gtattgcgag gagacagcag aagccatctg tgtgcttaag
      901 acatgccttg tgttaaattg catgcagttt aaacttgaga tggatgacct agcacataac
      961 gctgctgagc tggacaagat acagatgatg atacccttca gtgagcgtgt ttttaggatg
     1021 gcctcgtcct ttgcgactat tgatgcccag tgttttaggt tttgcgtgat gatgaaggat
     1081 aaaaatctga aaatagatat gcgtgaaacg acgagactgt ggactcgttc agcatcagat
     1141 gattctgtgg ccacgtcatc tttaagtatt tccctggacc ggggtcgatg ggtggcggct
     1201 gacgccagtg atgctagact gctggttttt ccgattcgcg tgtaatgggt gagtgagctg
     1261 atgtggtcgc caagacatgt gccggtgtct tggtggtggg tgacgcctaa tcatcgggtc
     1321 ggcatggcat ctccacctcc tcgcggtccg acctgggcat ccgaaggagg acgtcgtcca
     1381 ctcggatggc taagggagag ctcaaaaaaa aggatccggc tgctaacaaa gcccgaaagg
     1441 aagctgagtt ggctgctgcc accgctgagc aataactagc ataacccctt ggggcctcta
     1501 aacgggtctt gaggggtttt ttgctgaaag gaggaactat atccggatcg agatcctcta
     1561 gagtcgacct gcaggcatgc aagcttgtat tctatagtgt cacctaaatc gtatgtgtat
     1621 gatacataag gttatgtatt aattgtagcc gcgttctaac gacaatatgt acaagcctaa
     1681 ttgtgtagca tctggcttac tgaagcagac cctatcatct ctctcgtaaa ctgccgtcag
     1741 agtcggtttg gttggacgaa ccttctgagt ttctggtaac gccgttccgc accccggaaa
     1801 tggtcagcga accaatcagc agggtcatcg ctagccagat cctctacgcc ggacgcatcg
     1861 tggccggcat caccggcgcc acaggtgcgg ttgctggcgc ctatatcgcc gacatcaccg
     1921 atggggaaga tcgggctcgc cacttcgggc tcatgagcgc ttgtttcggc gtgggtatgg
     1981 tggcaggccc cgtggccggg ggactgttgg gcgccatctc cttgcaccat tccttgcggc
     2041 ggcggtgctc aacggcctca acctactact gggctgcttc ctaatgcagg agtcgcataa
     2101 gggagagcgt cgatatggtg cactctcagt acaatctgct ctgatgccgc atagttaagc
     2161 cagccccgac acccgccaac acccgctgac gcgccctgac gggcttgtct gctcccggca
     2221 tccgcttaca gacaagctgt gaccgtctcc gggagctgca tgtgtcagag gttttcaccg
     2281 tcatcaccga aacgcgcgag acgaaagggc ctcgtgatac gcctattttt ataggttaat
     2341 gtcatgataa taatggtttc ttagacgtca ggtggcactt ttcggggaaa tgtgcgcgga
     2401 acccctattt gtttattttt ctaaatacat tcaaatatgt atccgctcat gagacaataa
     2461 ccctgataaa tgcttcaata atattgaaaa aggaagagta tgagtattca acatttccgt
     2521 gtcgccctta ttcccttttt tgcggcattt tgccttcctg tttttgctca cccagaaacg
     2581 ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac gagtgggtta catcgaactg
     2641 gatctcaaca gcggtaagat ccttgagagt tttcgccccg aagaacgttt tccaatgatg
     2701 agcactttta aagttctgct atgtggcgcg gtattatccc gtattgacgc cgggcaagag
     2761 caactcggtc gccgcataca ctattctcag aatgacttgg ttgagtactc accagtcaca
     2821 gaaaagcatc ttacggatgg catgacagta agagaattat gcagtgctgc cataaccatg
     2881 agtgataaca ctgcggccaa cttacttctg acaacgatcg gaggaccgaa ggagctaacc
     2941 gcttttttgc acaacatggg ggatcatgta actcgccttg atcgttggga accggagctg
     3001 aatgaagcca taccaaacga cgagcgtgac accacgatgc ctgtagcaat ggcaacaacg
     3061 ttgcgcaaac tattaactgg cgaactactt actctagctt cccggcaaca attaatagac
     3121 tggatggagg cggataaagt tgcaggacca cttctgcgct cggcccttcc ggctggctgg
     3181 tttattgctg ataaatctgg agccggtgag cgtgggtctc gcggtatcat tgcagcactg
     3241 gggccagatg gtaagccctc ccgtatcgta gttatctaca cgacggggag tcaggcaact
     3301 atggatgaac gaaatagaca gatcgctgag ataggtgcct cactgattaa gcattggtaa
     3361 ctgtcagacc aagtttactc atatatactt tagattgatt taaaacttca tttttaattt
     3421 aaaaggatct aggtgaagat cctttttgat aatctcatga ccaaaatccc ttaacgtgag
     3481 ttttcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct
     3541 ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt
     3601 tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg
     3661 cagataccaa atactgtcct tctagtgtag ccgtagttag gccaccactt caagaactct
     3721 gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc
     3781 gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg
     3841 tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa
     3901 ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg
     3961 gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg
     4021 ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga
     4081 tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt
     4141 ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc gttatcccct
     4201 gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg ccgcagccga
     4261 acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcccaat acgcaaaccg
     4321 cctctccccg cgcgttggcc gattcattaa tgcaggggga t
//
