LOCUS       sequence-39512-e        4466 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4466)
  AUTHORS   Kobayashi T, Antar AA, Boehme KW, Danthi P, Eby EA, Guglielmi KM, 
            Holm GH, Johnson EM, Maginnis MS, Naik S, Skelton WB, Wetzel JD, 
            Wilson GJ, Chappell JD, Dermody TS
  TITLE     A plasmid-based reverse genetics system for animal double-stranded 
            RNA viruses.
  JOURNAL   Cell Host Microbe. 2007 Apr 19;1(2):147-57.
  PUBMED    18005692
REFERENCE   2  (bases 1 to 4466)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell Host 
            Microbe."; date: "2007-04-19"; volume: "1(2)"; pages: "147-57"
COMMENT     Sequence Label: pT7-S4T3D
FEATURES             Location/Qualifiers
     source          1..4466
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(1567..1585)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      1581..1628
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     promoter        complement(1696..1714)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(1697..1714)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(2005..2024)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     primer_bind     complement(2235..2254)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     2354..2376
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(2414..2432)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        2500..2604
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(2605..2673,2674..3465)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(2823..2842)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      3636..4224
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4125..4144
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     4378..4395
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 ctaggttgtt ctctatcttt gtgatgtggc tctgaaacaa accaggtgtg attacagtaa
       61 caatttcaat ttaaattccg atataaattt caatgagagg aaaattatta atcttcctca
      121 tagttattcg cacacaaaag atcctaaaaa ttcttctttc tttttgtgtg tccggggtcg
      181 gcatggcatc tccacctcct cgcgggttaa tacgactcac tatagctatt tttgcctctt
      241 cccagacgtt gtcgcaatgg aggtgtgctt gcccaacggt catcaggtcg tggacttgat
      301 taacaacgct tttgaaggtc gtgtatcaat ctacagcgcg caagagggat gggacaaaac
      361 aatctcagca cagccagata tgatggtatg tggtggcgcc gtcgtttgca tgcattgtct
      421 aggtgttgtc ggatctctac aacgcaagct gaagcatttg cctcaccata gatgtaatca
      481 acagatccgt catcaggatt acgtcgatgt acagttcgca gaccgtgtta ctgctcactg
      541 gaagcggggt atgctgtcct tcgttgcgca gatgcacgag atgatgaatg acgtgtcgcc
      601 agatgacctg gatcgtgtgc gtactgaggg aggttcacta gtggagctga actggcttca
      661 ggttgaccca aattcaatgt ttagatcaat acactcaagt tggacagatc ctttgcaggt
      721 ggtggacgac cttgacacta agctggatca gtactggaca gccttaaacc tgatgatcga
      781 ctcatccgac ttgataccca actttatgat gagagaccca tcacacgcgt tcaatggtgt
      841 gaaactgggg ggagatgctc gtcaaaccca attctccagg acttttgatt cgagatcgag
      901 tttggaatgg ggtgtgatgg tttatgatta ctctgagctg gagcatgatc catcgaaggg
      961 ccgtgcttac agaaaggaat tggtgacgcc agctcgagat ttcggtcact ttggattatc
     1021 ccattattct agggcgacta ccccaatcct tggaaagatg ccggccgtat tctcaggaat
     1081 gttgactggg aactgtaaaa tgtatccatt cattaaagga acggctaagc tgaagacagt
     1141 gcgcaagcta gtggaggcag tcaatcatgc ttggggtgtc gagaagatta gatatgctct
     1201 tgggccaggt ggcatgacgg gatggtacaa taggactatg caacaggccc ccattgtgct
     1261 aactcctgct gctctcacaa tgttcccaga taccatcaag tttggggatt tgaattatcc
     1321 agtgatgatt ggcgatccga tgattcttgg ctaaacaccc ccatcttcac agcgccgggc
     1381 ttgaccaacc tggtgtgacg tgggacaggc ttcattcatc gggtcggcat ggcatctcca
     1441 cctcctcgcg gtccgacctg ggcatccgaa ggaggacgtc gtccactcgg atggctaagg
     1501 gagagctcaa aaaaaaggat ccggctgcta acaaagcccg aaaggaagct gagttggctg
     1561 ctgccaccgc tgagcaataa ctagcataac cccttggggc ctctaaacgg gtcttgaggg
     1621 gttttttgct gaaaggagga actatatccg gatcgagatc ctctagagtc gacctgcagg
     1681 catgcaagct tgtattctat agtgtcacct aaatcgtatg tgtatgatac ataaggttat
     1741 gtattaattg tagccgcgtt ctaacgacaa tatgtacaag cctaattgtg tagcatctgg
     1801 cttactgaag cagaccctat catctctctc gtaaactgcc gtcagagtcg gtttggttgg
     1861 acgaaccttc tgagtttctg gtaacgccgt tccgcacccc ggaaatggtc agcgaaccaa
     1921 tcagcagggt catcgctagc cagatcctct acgccggacg catcgtggcc ggcatcaccg
     1981 gcgccacagg tgcggttgct ggcgcctata tcgccgacat caccgatggg gaagatcggg
     2041 ctcgccactt cgggctcatg agcgcttgtt tcggcgtggg tatggtggca ggccccgtgg
     2101 ccgggggact gttgggcgcc atctccttgc accattcctt gcggcggcgg tgctcaacgg
     2161 cctcaaccta ctactgggct gcttcctaat gcaggagtcg cataagggag agcgtcgata
     2221 tggtgcactc tcagtacaat ctgctctgat gccgcatagt taagccagcc ccgacacccg
     2281 ccaacacccg ctgacgcgcc ctgacgggct tgtctgctcc cggcatccgc ttacagacaa
     2341 gctgtgaccg tctccgggag ctgcatgtgt cagaggtttt caccgtcatc accgaaacgc
     2401 gcgagacgaa agggcctcgt gatacgccta tttttatagg ttaatgtcat gataataatg
     2461 gtttcttaga cgtcaggtgg cacttttcgg ggaaatgtgc gcggaacccc tatttgttta
     2521 tttttctaaa tacattcaaa tatgtatccg ctcatgagac aataaccctg ataaatgctt
     2581 caataatatt gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc ccttattccc
     2641 ttttttgcgg cattttgcct tcctgttttt gctcacccag aaacgctggt gaaagtaaaa
     2701 gatgctgaag atcagttggg tgcacgagtg ggttacatcg aactggatct caacagcggt
     2761 aagatccttg agagttttcg ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt
     2821 ctgctatgtg gcgcggtatt atcccgtatt gacgccgggc aagagcaact cggtcgccgc
     2881 atacactatt ctcagaatga cttggttgag tactcaccag tcacagaaaa gcatcttacg
     2941 gatggcatga cagtaagaga attatgcagt gctgccataa ccatgagtga taacactgcg
     3001 gccaacttac ttctgacaac gatcggagga ccgaaggagc taaccgcttt tttgcacaac
     3061 atgggggatc atgtaactcg ccttgatcgt tgggaaccgg agctgaatga agccatacca
     3121 aacgacgagc gtgacaccac gatgcctgta gcaatggcaa caacgttgcg caaactatta
     3181 actggcgaac tacttactct agcttcccgg caacaattaa tagactggat ggaggcggat
     3241 aaagttgcag gaccacttct gcgctcggcc cttccggctg gctggtttat tgctgataaa
     3301 tctggagccg gtgagcgtgg gtctcgcggt atcattgcag cactggggcc agatggtaag
     3361 ccctcccgta tcgtagttat ctacacgacg gggagtcagg caactatgga tgaacgaaat
     3421 agacagatcg ctgagatagg tgcctcactg attaagcatt ggtaactgtc agaccaagtt
     3481 tactcatata tactttagat tgatttaaaa cttcattttt aatttaaaag gatctaggtg
     3541 aagatccttt ttgataatct catgaccaaa atcccttaac gtgagttttc gttccactga
     3601 gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta
     3661 atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa
     3721 gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact
     3781 gtccttctag tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca
     3841 tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt
     3901 accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg
     3961 ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag
     4021 cgtgagctat gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta
     4081 agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat
     4141 ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg
     4201 tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc
     4261 ttttgctggc cttttgctca catgttcttt cctgcgttat cccctgattc tgtggataac
     4321 cgtattaccg cctttgagtg agctgatacc gctcgccgca gccgaacgac cgagcgcagc
     4381 gagtcagtga gcgaggaagc ggaagagcgc ccaatacgca aaccgcctct ccccgcgcgt
     4441 tggccgattc attaatgcag ggggat
//
