LOCUS       sequence-395127-       11830 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  This plasmid expresses the Cas9-CtIP-dnRNF168 in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11830)
  AUTHORS   Carusillo A, Haider S, Schafer R, Rhiel M, Turk D, Chmielewski KO, 
            Klermund J, Mosti L, Andrieux G, Schafer R, Cornu TI, Cathomen T, 
            Mussolino C
  TITLE     A novel Cas9 fusion protein promotes targeted genome editing with 
            reduced mutational burden in primary human cells.
  JOURNAL   Nucleic Acids Res. 2023 May 22;51(9):4660-4673. doi: 
            10.1093/nar/gkad255.
  PUBMED    37070192
REFERENCE   2  (bases 1 to 11830)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10"; journalName: 
            "Nucleic Acids Res"; date: "2023-05-22- 22"; volume: "51"; issue: 
            "9"; pages: "4660-4673"
COMMENT     Sequence Label: 2333_Cas9-CtIP-dnRNF168
FEATURES             Location/Qualifiers
     source          1..11830
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        34..413
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        414..617
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     568..588
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     614..638
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     promoter        848..866
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     848..865
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     868..887
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        868..886
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     901..920
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     regulatory      973..982
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             985..1011
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             1096..1116
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             1126..5226
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="DKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
                     LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
                     LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
                     RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE
                     NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
                     GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
                     QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR
                     KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
                     SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
                     TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
                     VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
                     KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
                     LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
                     KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
                     YYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS
                     EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
                     AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
                     AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
                     ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
                     SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
                     IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNEL
                     ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
                     NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD
                     ATLIHQSITGLYETRIDLSQLGGD"
     CDS             1126..5226
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="DKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
                     LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
                     LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
                     RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE
                     NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
                     GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
                     QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR
                     KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
                     SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
                     TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
                     VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
                     KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
                     LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
                     KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
                     YYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS
                     EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
                     AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
                     AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
                     ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
                     SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
                     IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNEL
                     ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
                     NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD
                     ATLIHQSITGLYETRIDLSQLGGD"
     CDS             5269..5289
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             6678..6689
                     /codon_start=1
                     /product="WELQut protease recognition and cleavage site"
                     /label=WELQut site
                     /translation="WELQ"
     primer_bind     complement(9653..9670)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    9659..9883
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     CDS             10056..10850
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             10056..10850
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(10110..10129)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     10720..10739
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     rep_origin      11175..11763
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     11664..11683
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 tgcttcgcga tgtacgggcc agatatacgc gttgacattg attattgact agttattaat
       61 agtaatcaat tacggggtca ttagttcata gcccatatat ggagttccgc gttacataac
      121 ttacggtaaa tggcccgcct ggctgaccgc ccaacgaccc ccgcccattg acgtcaataa
      181 tgacgtatgt tcccatagta acgccaatag ggactttcca ttgacgtcaa tgggtggagt
      241 atttacggta aactgcccac ttggcagtac atcaagtgta tcatatgcca agtacgcccc
      301 ctattgacgt caatgacggt aaatggcccg cctggcatta tgcccagtac atgaccttat
      361 gggactttcc tacttggcag tacatctacg tattagtcat cgctattacc atggtgatgc
      421 ggttttggca gtacatcaat gggcgtggat agcggtttga ctcacgggga tttccaagtc
      481 tccaccccat tgacgtcaat gggagtttgt tttggcacca aaatcaacgg gactttccaa
      541 aatgtcgtaa caactccgcc ccattgacgc aaatgggcgg taggcgtgta cggtgggagg
      601 tctatataag cagagctcgt ttagtgaacc gtcagatcgc ctggagacgc catccacgct
      661 gttttgacct ccatagatga caccgggacc gatccagcct ccgcggccgg gaacggtgca
      721 ttggaacgcg gattccccgt gccaagagtg acgtaagtac cgcctataga gtctataggc
      781 ccaccccctt ggcttcgtta gaacgcggct acaattaata cataacctta tgtatcatac
      841 acatacgatt taggtgacac tatagaataa tacgactcac tatagggagt aacatccact
      901 ttgcctttct ctccacaggt gtccactccc aggtccaact gcacctcggt tctatcgatt
      961 gaattcaagg aggccaccat ggaatatcca tacgacgtac ccgattacgc ggcgcgcctt
     1021 ggtagtggtt accggtcagc cgcgggaggt ggcggaagcg gtaaaagaac tgccgacgga
     1081 tcagagtttg aatctccgaa aaaaaagaga aaagtcggag ctagcgacaa gaagtacagc
     1141 atcggcctgg acatcggcac caactctgtg ggctgggccg tgatcaccga cgagtacaag
     1201 gtgcccagca agaaattcaa ggtgctgggc aacaccgacc ggcacagcat caagaagaac
     1261 ctgatcggag ccctgctgtt cgacagcggc gaaacagccg aggccacccg gctgaagaga
     1321 accgccagaa gaagatacac cagacggaag aaccggatct gctatctgca agagatcttc
     1381 agcaacgaga tggccaaggt ggacgacagc ttcttccaca gactggaaga gtccttcctg
     1441 gtggaagagg ataagaagca cgagcggcac cccatcttcg gcaacatcgt ggacgaggtg
     1501 gcctaccacg agaagtaccc caccatctac cacctgagaa agaaactggt ggacagcacc
     1561 gacaaggccg acctgcggct gatctatctg gccctggccc acatgatcaa gttccggggc
     1621 cacttcctga tcgagggcga cctgaacccc gacaacagcg acgtggacaa gctgttcatc
     1681 cagctggtgc agacctacaa ccagctgttc gaggaaaacc ccatcaacgc cagcggcgtg
     1741 gacgccaagg ccatcctgtc tgccagactg agcaagagca gacggctgga aaatctgatc
     1801 gcccagctgc ccggcgagaa gaagaatggc ctgttcggaa acctgattgc cctgagcctg
     1861 ggcctgaccc ccaacttcaa gagcaacttc gacctggccg aggatgccaa actgcagctg
     1921 agcaaggaca cctacgacga cgacctggac aacctgctgg cccagatcgg cgaccagtac
     1981 gccgacctgt ttctggccgc caagaacctg tccgacgcca tcctgctgag cgacatcctg
     2041 agagtgaaca ccgagatcac caaggccccc ctgagcgcct ctatgatcaa gagatacgac
     2101 gagcaccacc aggacctgac cctgctgaaa gctctcgtgc ggcagcagct gcctgagaag
     2161 tacaaagaga ttttcttcga ccagagcaag aacggctacg ccggctacat tgacggcgga
     2221 gccagccagg aagagttcta caagttcatc aagcccatcc tggaaaagat ggacggcacc
     2281 gaggaactgc tcgtgaagct gaacagagag gacctgctgc ggaagcagcg gaccttcgac
     2341 aacggcagca tcccccacca gatccacctg ggagagctgc acgccattct gcggcggcag
     2401 gaagattttt acccattcct gaaggacaac cgggaaaaga tcgagaagat cctgaccttc
     2461 cgcatcccct actacgtggg ccctctggcc aggggaaaca gcagattcgc ctggatgacc
     2521 agaaagagcg aggaaaccat caccccctgg aacttcgagg aagtggtgga caagggcgct
     2581 tccgcccaga gcttcatcga gcggatgacc aacttcgata agaacctgcc caacgagaag
     2641 gtgctgccca agcacagcct gctgtacgag tacttcaccg tgtataacga gctgaccaaa
     2701 gtgaaatacg tgaccgaggg aatgagaaag cccgccttcc tgagcggcga gcagaaaaag
     2761 gccatcgtgg acctgctgtt caagaccaac cggaaagtga ccgtgaagca gctgaaagag
     2821 gactacttca agaaaatcga gtgcttcgac tccgtggaaa tctccggcgt ggaagatcgg
     2881 ttcaacgcct ccctgggcac ataccacgat ctgctgaaaa ttatcaagga caaggacttc
     2941 ctggacaatg aggaaaacga ggacattctg gaagatatcg tgctgaccct gacactgttt
     3001 gaggacagag agatgatcga ggaacggctg aaaacctatg cccacctgtt cgacgacaaa
     3061 gtgatgaagc agctgaagcg gcggagatac accggctggg gcaggctgag ccggaagctg
     3121 atcaacggca tccgggacaa gcagtccggc aagacaatcc tggatttcct gaagtccgac
     3181 ggcttcgcca acagaaactt catgcagctg atccacgacg acagcctgac ctttaaagag
     3241 gacatccaga aagcccaggt gtccggccag ggcgatagcc tgcacgagca cattgccaat
     3301 ctggccggca gccccgccat taagaagggc atcctgcaga cagtgaaggt ggtggacgag
     3361 ctcgtgaaag tgatgggccg gcacaagccc gagaacatcg tgatcgaaat ggccagagag
     3421 aaccagacca cccagaaggg acagaagaac agccgcgaga gaatgaagcg gatcgaagag
     3481 ggcatcaaag agctgggcag ccagatcctg aaagaacacc ccgtggaaaa cacccagctg
     3541 cagaacgaga agctgtacct gtactacctg cagaatgggc gggatatgta cgtggaccag
     3601 gaactggaca tcaaccggct gtccgactac gatgtggacc atatcgtgcc tcagagcttt
     3661 ctgaaggacg actccatcga caacaaggtg ctgaccagaa gcgacaagaa ccggggcaag
     3721 agcgacaacg tgccctccga agaggtcgtg aagaagatga agaactactg gcggcagctg
     3781 ctgaacgcca agctgattac ccagagaaag ttcgacaatc tgaccaaggc cgagagaggc
     3841 ggcctgagcg aactggataa ggccggcttc atcaagagac agctggtgga aacccggcag
     3901 atcacaaagc acgtggcaca gatcctggac tcccggatga acactaagta cgacgagaat
     3961 gacaagctga tccgggaagt gaaagtgatc accctgaagt ccaagctggt gtccgatttc
     4021 cggaaggatt tccagtttta caaagtgcgc gagatcaaca actaccacca cgcccacgac
     4081 gcctacctga acgccgtcgt gggaaccgcc ctgatcaaaa agtaccctaa gctggaaagc
     4141 gagttcgtgt acggcgacta caaggtgtac gacgtgcgga agatgatcgc caagagcgag
     4201 caggaaatcg gcaaggctac cgccaagtac ttcttctaca gcaacatcat gaactttttc
     4261 aagaccgaga ttaccctggc caacggcgag atccggaagc ggcctctgat cgagacaaac
     4321 ggcgaaaccg gggagatcgt gtgggataag ggccgggatt ttgccaccgt gcggaaagtg
     4381 ctgagcatgc cccaagtgaa tatcgtgaaa aagaccgagg tgcagacagg cggcttcagc
     4441 aaagagtcta tcctgcccaa gaggaacagc gataagctga tcgccagaaa gaaggactgg
     4501 gaccctaaga agtacggcgg cttcgacagc cccaccgtgg cctattctgt gctggtggtg
     4561 gccaaagtgg aaaagggcaa gtccaagaaa ctgaagagtg tgaaagagct gctggggatc
     4621 accatcatgg aaagaagcag cttcgagaag aatcccatcg actttctgga agccaagggc
     4681 tacaaagaag tgaaaaagga cctgatcatc aagctgccta agtactccct gttcgagctg
     4741 gaaaacggcc ggaagagaat gctggcctct gccggcgaac tgcagaaggg aaacgaactg
     4801 gccctgccct ccaaatatgt gaacttcctg tacctggcca gccactatga gaagctgaag
     4861 ggctcccccg aggataatga gcagaaacag ctgtttgtgg aacagcacaa gcactacctg
     4921 gacgagatca tcgagcagat cagcgagttc tccaagagag tgatcctggc cgacgctaat
     4981 ctggacaaag tgctgtccgc ctacaacaag caccgggata agcccatcag agagcaggcc
     5041 gagaatatca tccacctgtt taccctgacc aatctgggag cccctgccgc cttcaagtac
     5101 tttgacacca ccatcgaccg gaagaggtac accagcacca aagaggtgct ggacgccacc
     5161 ctgatccacc agagcatcac cggcctgtac gagacacgga tcgacctgtc tcagctggga
     5221 ggcgacctcg agggtaaacg aacagcggac ggttctgagt tcgagagccc gaaaaaaaaa
     5281 aggaaggtaa gtgccgcagg cggtgggggt agtggtccta ggatggctct acccaaagac
     5341 gccatcccct cgctgtccga gtgctcgtcg tggactcggt accatacccg aagaaattct
     5401 ctcgtcaacg tggaactgtg gacgataatt caaaaacact atcccaggga gtgcaagctt
     5461 agagcgtctg gccaagaatc agaggaagtg gctgatgact atcagccagt tcgtctgctc
     5521 agtaaacctg gggaactgag aagagaatat gaagaggaaa taagcaaggt ggcggcagag
     5581 cgacgggcca gcgaggaaga agaaaacaaa gccagtgaag aatacataca gaggttgttg
     5641 gcagaggagg aagaagagga aaaaagacag gcagaaaaaa ggcgaagagc gatggaagaa
     5701 caactgaaaa gtgatgagga actggcaaga aagctaagca ttgatattaa caatttctgt
     5761 gagggaagta tctcggcttc tcccttgaat tccagaaaat ctgatccagt tacacccaag
     5821 tctgaaaaga aaagtaagaa caaacaaaga aacactggag atattcagaa gtatttgaca
     5881 ccgaaatctc agtttgggtc agcctcacac tctgaagctg tacaagaagt caggaaagac
     5941 tccgtatcta aggacattga cagtagtgat aggaaaagcc caacagggca agacacagaa
     6001 atagaagata tgccgacact ttctccacag atatcccttg gagttggaga acaaggtgca
     6061 gattcttcaa tagagtcccc tatgccatgg ttatgtgcct gtggtgccga atggtaccat
     6121 gaaggaaacg tcaaaacaag accaagcaat catgggaaag agttatgtgt cttaagtcac
     6181 gagcgaccta aaaccagagt tccctactcg aaagaaactg cagttatgcc ttgtggcaga
     6241 acagaaagtg ggtgcgcccc cacatcaggg gtgacacaga caaatggaaa caacacaggt
     6301 gagacagaaa atgaagagtc gtgcctactg atcagtaagg agatttccaa aagaaaaaac
     6361 caagaatctt cctttgaagc agtcaaggat caatgctttt ctgcaaaaag aagaaaagtg
     6421 tcccccgaat cttccccaga tcaagaggaa acagaaataa actttaccca aaaactgata
     6481 gatttggagc atctactgtt tgagagacat aaacaagaag aacaggacag gttattggca
     6541 ttacaacttc agaaggaggt ggataaagag caaatggtgc caaaccggca aaaaggatcc
     6601 ccagatgagt atcacttacg cgctacatcc tcccctccag acaaagtgct aaatggacag
     6661 aggaagaatc ccaaagatgg gaacttcaaa aggcaaactc acacaaagca tccaacacca
     6721 gagagaggct caagggacaa aaataggcaa gtgtctttaa agatgcagtt gaagcagtca
     6781 gttaatagaa gaaagatgcc aaattctact agagatcact gtaaggtatc caaaagtgct
     6841 cactccctac agcctagcat ttcacagaaa agtgtttttc agatgtttca gagatgcaca
     6901 aaggtcgaca gtgccgcagg cggtgggggt agtggtccta gtatgaacat ctcgggaagc
     6961 agctgtggaa gccctaactc tgcagataca tctagtgact ttaaggacct ttggacaaaa
     7021 ctaaaagaat gtcatgatag agaagtacaa ggtttacaag taaaagtaac caagctaaaa
     7081 caggaacgaa tcttagatgc acaaagacta gaagaattct tcaccaaaaa tcaacagctg
     7141 agggaacagc agaaagtcct tcatgaaacc attaaagttt tagaagatcg gttaagagca
     7201 ggcttatgtg atcgctgtgc agtaactgaa gaacatatgc ggaaaaaaca gcaagagttt
     7261 gaaaatatcc ggcagcagaa tcttaaactt attacagaac ttatgaatga aaggaatact
     7321 ctacaggaag aaaataaaaa gctttctgaa caactccagc agaaaattga gaatgatcaa
     7381 cagcatcaag cagctgagct tgaatgtgag gaagacgtta ttccagattc accgataaca
     7441 gccttctcat tttctggcgt taaccggcta cgaagaaagg agaaccccca tgtccgatac
     7501 atagaacaaa cacatactaa attggagcac tctgtgtgtg caaatgaaat gagaaaagtt
     7561 tccaagtctt caactcatcc acaacataat cctaatgaaa atgaaattct agtagctgac
     7621 acttatgacc aaagtcaatc tccaatggcc aaagcacatg gaacaagcag ctatacccct
     7681 gataagtcat cttttaattt agctacagtt gttgctgaaa cacttggact tggtgttcaa
     7741 gaagaatctg aaactcaagg tcccatgagc ccccttggtg atgagctcta ccactgtctg
     7801 gaaggaaatc acaagaaaca gccttttgag gaatctacaa gaaatactga agatagttta
     7861 agattttcag attctacttc aaagactcct cctcaagaag aattacctac tcgagtgtca
     7921 tctcctgtat ttggagctac ctctagtatc aaaagtggtt tagatttgaa tacaagtttg
     7981 tccccttctc ttttacagcc tgggaaaaaa aaacatctga aaacactccc ttttagcaac
     8041 acttgtatat ctagattaga aaaaactaga tcaaaatctg aagatagtgc ccttttcaca
     8101 catcacagtc ttgggtctga agtgaacaag atcattatcc agtcatctaa taaacagata
     8161 cttataaata aaaatataag tgaatcccta ggtgaacaga ataggactga gtacggtaaa
     8221 gattctaaca ctgataaaca tttggagccc ctgaaatcat tgggaggccg aacatccaaa
     8281 aggaagaaaa ctgaggaaga aagtgaacat gaagtaagct gcccccaagc ttcttttgat
     8341 aaagaaaatg ctttcccttt tccaatggat aatcagtttt ccatgaatgg agactgtgtg
     8401 atggataaac ctctggatct gtctgatcga ttttcagcta ttcagcgtca agagaaaagc
     8461 caaggaagtg agacttctaa aaacaaattt aggcaagtga ctctttatga ggctttgaag
     8521 accattccaa agggcttttc ctcaagccgt aaggcctcag atggcaactg cacgttgccc
     8581 aaagattccc caggggagcc ctgttcacag gaatgcatca tccttcagcc cttgaataaa
     8641 tgctctccag acaataaacc atcattacaa ataaaagaag aaaatgctgt ctttaaaatt
     8701 cctctacgtc cacgtgaaag tttggagact gagaatgttt tagatgacat aaagagtgct
     8761 ggttctcatg agccaataaa aatacaaacc aggtcagacc atggaggatg tgaacttgca
     8821 tcagttcttc agttaaatcc atgtagaact ggtaaaataa agtctctaca aaacaaccaa
     8881 gatgtatcct ttgaaaatat ccagtggagt atagatccgg gagcagacct ttctcagtat
     8941 aaaatggatg ttactgtaat agatacaaag gatggcagtc agtcaaaatt aggaggagag
     9001 acagtggaca tggactgtac attggttagt gaaaccgttc tcttaaaaat gaagaagcaa
     9061 gagcagaagg gagaaaaaag ttcaaatgaa gaaagaaaaa tgaatgatag cttggaagat
     9121 atgtttgatc ggacaacaca tgaagagtat gaatcctgtt tggcagacag tttctcccaa
     9181 gcagcagatg aagaggagga attgtctact gccacaaaga aactacacac tcatggtgat
     9241 aaacaagaca aagtcaagca gaaagcgttt gtggagccgt attttaaagg tgatgaaaga
     9301 gagactagct tgcaaaattt tcctcatatt gaggtggttc ggaaaaaaga ggagagaaga
     9361 aaactgcttg ggcacacgtg taaggaatgt gaaatttatt atgcagatat gccagcagaa
     9421 gaaagagaaa agaaattggc ttcctgctca agacaccgat tccgctacat tccacccaac
     9481 acaccagaga atttttggga agttggtttt ccttccactc agacttgtat ggaaagaggt
     9541 tatattaagg aagatcttga tccttgtcct cgtccaaaaa gacgtcagcc ttacaacgca
     9601 atattttctc caaaaggcaa ggagcagaag acagtttaaa cccgctgatc agcctcgact
     9661 gtgccttcta gttgccagcc atctgttgtt tgcccctccc ccgtgccttc cttgaccctg
     9721 gaaggtgcca ctcccactgt cctttcctaa taaaatgagg aaattgcatc gcattgtctg
     9781 agtaggtgtc attctattct ggggggtggg gtggggcagg acagcaaggg ggaggattgg
     9841 gaagaaaata gcaggcatgc tggggatgcg gtgggctcta tggcttctac tgggcggttt
     9901 tatggacagc aagcgaaccg gaattgccag ctggggcgcc ctctggtaag gttgggaagc
     9961 cctgcaaagt aaactggatg gctttcttgc cgccaaggat ctgatggcgc aggggatcaa
    10021 gctctgatca agagacagga tgaggatcgt ttcgcatgat tgaacaagat ggattgcacg
    10081 caggttctcc ggccgcttgg gtggagaggc tattcggcta tgactgggca caacagacaa
    10141 tcggctgctc tgatgccgcc gtgttccggc tgtcagcgca ggggcgcccg gttctttttg
    10201 tcaagaccga cctgtccggt gccctgaatg aactgcaaga cgaggcagcg cggctatcgt
    10261 ggctggccac gacgggcgtt ccttgcgcag ctgtgctcga cgttgtcact gaagcgggaa
    10321 gggactggct gctattgggc gaagtgccgg ggcaggatct cctgtcatct caccttgctc
    10381 ctgccgagaa agtatccatc atggctgatg caatgcggcg gctgcatacg cttgatccgg
    10441 ctacctgccc attcgaccac caagcgaaac atcgcatcga gcgagcacgt actcggatgg
    10501 aagccggtct tgtcgatcag gatgatctgg acgaagagca tcaggggctc gcgccagccg
    10561 aactgttcgc caggctcaag gcgagcatgc ccgacggcga ggatctcgtc gtgacccatg
    10621 gcgatgcctg cttgccgaat atcatggtgg aaaatggccg cttttctgga ttcatcgact
    10681 gtggccggct gggtgtggcg gaccgctatc aggacatagc gttggctacc cgtgatattg
    10741 ctgaagagct tggcggcgaa tgggctgacc gcttcctcgt gctttacggt atcgccgctc
    10801 ccgattcgca gcgcatcgcc ttctatcgcc ttcttgacga gttcttctga attattaacg
    10861 cttacaattt cctgatgcgg tattttctcc ttacgcatct gtgcggtatt tcacaccgca
    10921 tcaggtggca cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt tttctaaata
    10981 cattcaaata tgtatccgct catgagacaa taaccctgat aaatgcttca ataatagcac
    11041 gtgctaaaac ttcattttta atttaaaagg atctaggtga agatcctttt tgataatctc
    11101 atgaccaaaa tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag
    11161 atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa
    11221 aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg
    11281 aaggtaactg gcttcagcag agcgcagata ccaaatactg ttcttctagt gtagccgtag
    11341 ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg
    11401 ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga
    11461 tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc
    11521 ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc
    11581 acgcttcccg aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga
    11641 gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt
    11701 cgccacctct gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg
    11761 aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac
    11821 atgttcttgc
//
