LOCUS       sequence-39513-e        6929 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6929)
  AUTHORS   Kobayashi T, Antar AA, Boehme KW, Danthi P, Eby EA, Guglielmi KM, 
            Holm GH, Johnson EM, Maginnis MS, Naik S, Skelton WB, Wetzel JD, 
            Wilson GJ, Chappell JD, Dermody TS
  TITLE     A plasmid-based reverse genetics system for animal double-stranded 
            RNA viruses.
  JOURNAL   Cell Host Microbe. 2007 Apr 19;1(2):147-57.
  PUBMED    18005692
REFERENCE   2  (bases 1 to 6929)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell Host 
            Microbe."; date: "2007-04-19"; volume: "1(2)"; pages: "147-57"
COMMENT     Sequence Label: pT7-L1T1L
FEATURES             Location/Qualifiers
     source          1..6929
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(4030..4048)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      4044..4091
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     promoter        complement(4159..4177)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(4160..4177)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(4468..4487)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     primer_bind     complement(4698..4717)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     4817..4839
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(4877..4895)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        4963..5067
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5068..5136,5137..5928)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5286..5305)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      6099..6687
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6588..6607
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     6841..6858
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 atcgatcccg ggtaatacga ctcactatag ctacacgttc cacgacaatg tcatccatga
       61 tactgactca gtttagaccg ttcattgaaa gcatttcagg tatcactgac caatcgaatg
      121 acgtgtttga agatgcagca aaagcattct ctatgtttac tcgcagcgac gtctacaagg
      181 cgctggatga aatacctttc tctgatgatg cgatgcttcc catccctcca actatatata
      241 ctaaaccatc tcacgattca tattattaca ttgatgctct aaaccgtgtg cgtcgcaaaa
      301 catatcaggg ccctgatgac gtgtacgtac ctaattgttc tattgttgaa ttgctggagc
      361 cacatgagac tctgacatct tatgggcgtt tgtctgaggc catcgagaat cgtgccaagg
      421 atggagacag ccaagccaga atcgcaacga cgtatggtag aatcgctgaa tctcaagctc
      481 gacagattaa ggctccattg gagaagtttg tgttggcact actagtgtcc gaagcggggg
      541 ggtctttata tgatccagtt ctgcagaagt atgatgagat tccagatcta tcgcataatt
      601 gccctttatg gtgttttaga gaaatctgtc gtcatatatc tggtccatta ccagaccgag
      661 caccttatct ttacttatct gcaggggttt tctggttaat gtcaccacga atgacgtctg
      721 caatccctcc gttactatcc gatcttgtta atttagctat tttacaacag actgcgggtt
      781 tggatccatc attagtgaaa ttgggagtgc agatatgcct tcatgcggca gctagctcaa
      841 gttatgcatg gtttatctta aagactaagt ctatttttcc tcaaaacacg ttacatagta
      901 tgtatgaatc tctagaaggg gggtattgtc ctaaccttga atggttagag cctagatcgg
      961 actataaatt catgtacatg ggagtcatgc cattgtccac taagtatgct aggtcggcgc
     1021 cgtcaaatga taagaaagcg cgggaacttg gcgagaaata tggactgagc tcagtcgtca
     1081 gtgagcttcg taaacggaca aagacttatg ttaaacatga ctttgcttcg gtaaggtaca
     1141 ttcgtgacgc tatggcatgt actagcggta ttttcttggt aagaacaccc accgaaacgg
     1201 tattgcaaga atatactcag agtccggaga ttaaggttcc catcccccaa aaagattgga
     1261 caggcccagt aggtgagatc agaattctaa aagacacaac aagctccatc gcgcgctact
     1321 tatatagaac atggtactta gcagcggcaa gaatggcggc tcaaccacgt acgtgggatc
     1381 cattgtttca ggcgattatg agatctcaat acgtgacagc taggggtgga tctggcgcag
     1441 cactccgcga gtctctgtac gcaattaatg tgtcgttacc tgattttaag ggcttaccag
     1501 tgaaggcagc aactaagata tttcaggcgg cacaattagc gaacttgccg ttctcccaca
     1561 catcagtggc tatactagct gacacttcga tgggattgcg aaaccaggtg cagaggcggc
     1621 cacgatccat tatgccctta aatgtgcccc aacagcaggt ttcggcgcct catacattga
     1681 ccgcggatta catcaattac cacatgaacc tatcaaccac gtctggtagc gcggtcattg
     1741 agaaagtgat tcccttaggt gtatacgctt cgagccctcc taaccaatcg attaacattg
     1801 acatatctgc atgtgacgca agtattactt gggatttctt tctatccgtg attatggcgg
     1861 ctatacacga aggtgtcgct agtagctcca ttggaaaacc gtttatgggg gttcctgcat
     1921 ccattgtaaa tgatgagtct gtcgttggag tgagagctgc taggccgata tcgggaatgc
     1981 agaacatgat tcagcatcta tcaaaactgt ataaacgtgg attttcatac agagtgaacg
     2041 actctttttc tccaggtaac gactttaccc atatgactac cactttcccg tcaggttcaa
     2101 cagccacctc tactgagcat actgctaata atagtacgat gatggaaact ttcctgacag
     2161 tatggggacc cgaacatact gacgaccccg acgtcttacg tctaatgaag tctttaacta
     2221 ttcaaaggaa ttacgtgtgt caaggtgatg atggattgat gattatcgat gggaatactg
     2281 ctggtaaggt gaacagtgaa actattcaga agatgctgga gttaatctca aaatatggtg
     2341 aggaatttgg atggaaatat gacatagcgt acgatgggac tgccgaatac ctaaagctgt
     2401 atttcatatt tggctgtcga attccaaatc ttagtcgtca tccaatcgtg gggaaagaac
     2461 gggcgaattc ttcagcagag gagccatggc cagcaattct agaccagatt atgggtatct
     2521 tctttaatgg cgttcatgac gggttacagt ggcagcggtg gatacgttat tcatgggctc
     2581 tatgctgtgc tttctcacgt caaagaacaa tgattggtga gagcgtaggt taccttcaat
     2641 atcctatgtg gtcttttgtc tactggggat taccactggt taaagtgttc gggtcagacc
     2701 catggatatt ttcttggtac atgcctactg gggacctggg aatgtatagt tggattagcc
     2761 tgatacgccc tctaatgaca agatggatgg tggctaatgg ttacgcaact gacagatgct
     2821 cacccgtatt cgggaacgca gattatcgca ggtgtttcaa tgaaattaaa ctatatcaag
     2881 ggtattatat ggcacaattg cctaggaatc ctacgaaatc tggacgagcg gcccctcggg
     2941 aggtaagaga acaattcact caggcattat ccgattatct gatgcaaaat ccagagctga
     3001 agtcacgtgt gctacgtggt cgtagtgagt gggagaagta tggagcgggg ataattcaca
     3061 atcctccatc attattcgat gtgccccata agtggtatct gggtgcgcaa gaggcggcaa
     3121 ccgctacgag agaagagctg gcagaaatgg atgagacatt aatgcgcgct cgaaggcaca
     3181 gttattcaag cttttcaaag ttgttggagg catacctgct cgtgaaatgg cgaatgtgcg
     3241 aggcccgcga accgtcggtt gatttgcgat taccattatg tgcgggtatt gacccactaa
     3301 actcagatcc ttttctcaag atggtaagcg ttgggccaat gctccagagt acgagaaagt
     3361 actttgctca gacactattc atggcaaaga cggtgtcggg tctcgacgtt aacgcgattg
     3421 acagcgcgtt attacgactg cgaacattgg gtgctgataa gaaagcatta acagcgcagt
     3481 tattaatggt ggggcttcag gagtcagaag ctgacgcgtt ggctgggaag ataatgctac
     3541 aagatgtaag tactgtgcaa ttagccagag tggtcaactt agctgtgcca gatacttgga
     3601 tgtcgttgga ctttgactct atgttcaaac accatgttaa gttgcttccc aaagatggac
     3661 gtcatctaaa tactgatatt cctcctcgaa tgggatggtt acgggccatt ttacgattct
     3721 taggtgctgg aatggtaatg actgcgactg gagttgctgt cgacatatat ctggaggata
     3781 tacacggcgg tggtcgagca cttggacaga gattcatgac ttggatgcga caggaaggac
     3841 ggtcagcgtg agtctaccat gggtcgtggt gcgtcaactc atcgggtcgg catggcatct
     3901 ccacctcctc gcggtccgac ctgggcatcc gaaggaggac gtcgtccact cggatggcta
     3961 agggagagct caaaaaaaag gatccggctg ctaacaaagc ccgaaaggaa gctgagttgg
     4021 ctgctgccac cgctgagcaa taactagcat aaccccttgg ggcctctaaa cgggtcttga
     4081 ggggtttttt gctgaaagga ggaactatat ccggatcgag atcctctaga gtcgacctgc
     4141 aggcatgcaa gcttgtattc tatagtgtca cctaaatcgt atgtgtatga tacataaggt
     4201 tatgtattaa ttgtagccgc gttctaacga caatatgtac aagcctaatt gtgtagcatc
     4261 tggcttactg aagcagaccc tatcatctct ctcgtaaact gccgtcagag tcggtttggt
     4321 tggacgaacc ttctgagttt ctggtaacgc cgttccgcac cccggaaatg gtcagcgaac
     4381 caatcagcag ggtcatcgct agccagatcc tctacgccgg acgcatcgtg gccggcatca
     4441 ccggcgccac aggtgcggtt gctggcgcct atatcgccga catcaccgat ggggaagatc
     4501 gggctcgcca cttcgggctc atgagcgctt gtttcggcgt gggtatggtg gcaggccccg
     4561 tggccggggg actgttgggc gccatctcct tgcaccattc cttgcggcgg cggtgctcaa
     4621 cggcctcaac ctactactgg gctgcttcct aatgcaggag tcgcataagg gagagcgtcg
     4681 atatggtgca ctctcagtac aatctgctct gatgccgcat agttaagcca gccccgacac
     4741 ccgccaacac ccgctgacgc gccctgacgg gcttgtctgc tcccggcatc cgcttacaga
     4801 caagctgtga ccgtctccgg gagctgcatg tgtcagaggt tttcaccgtc atcaccgaaa
     4861 cgcgcgagac gaaagggcct cgtgatacgc ctatttttat aggttaatgt catgataata
     4921 atggtttctt agacgtcagg tggcactttt cggggaaatg tgcgcggaac ccctatttgt
     4981 ttatttttct aaatacattc aaatatgtat ccgctcatga gacaataacc ctgataaatg
     5041 cttcaataat attgaaaaag gaagagtatg agtattcaac atttccgtgt cgcccttatt
     5101 cccttttttg cggcattttg ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta
     5161 aaagatgctg aagatcagtt gggtgcacga gtgggttaca tcgaactgga tctcaacagc
     5221 ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc caatgatgag cacttttaaa
     5281 gttctgctat gtggcgcggt attatcccgt attgacgccg ggcaagagca actcggtcgc
     5341 cgcatacact attctcagaa tgacttggtt gagtactcac cagtcacaga aaagcatctt
     5401 acggatggca tgacagtaag agaattatgc agtgctgcca taaccatgag tgataacact
     5461 gcggccaact tacttctgac aacgatcgga ggaccgaagg agctaaccgc ttttttgcac
     5521 aacatggggg atcatgtaac tcgccttgat cgttgggaac cggagctgaa tgaagccata
     5581 ccaaacgacg agcgtgacac cacgatgcct gtagcaatgg caacaacgtt gcgcaaacta
     5641 ttaactggcg aactacttac tctagcttcc cggcaacaat taatagactg gatggaggcg
     5701 gataaagttg caggaccact tctgcgctcg gcccttccgg ctggctggtt tattgctgat
     5761 aaatctggag ccggtgagcg tgggtctcgc ggtatcattg cagcactggg gccagatggt
     5821 aagccctccc gtatcgtagt tatctacacg acggggagtc aggcaactat ggatgaacga
     5881 aatagacaga tcgctgagat aggtgcctca ctgattaagc attggtaact gtcagaccaa
     5941 gtttactcat atatacttta gattgattta aaacttcatt tttaatttaa aaggatctag
     6001 gtgaagatcc tttttgataa tctcatgacc aaaatccctt aacgtgagtt ttcgttccac
     6061 tgagcgtcag accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc
     6121 gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat
     6181 caagagctac caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat
     6241 actgtccttc tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct
     6301 acatacctcg ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt
     6361 cttaccgggt tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg
     6421 gggggttcgt gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta
     6481 cagcgtgagc tatgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg
     6541 gtaagcggca gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg
     6601 tatctttata gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc
     6661 tcgtcagggg ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg
     6721 gccttttgct ggccttttgc tcacatgttc tttcctgcgt tatcccctga ttctgtggat
     6781 aaccgtatta ccgcctttga gtgagctgat accgctcgcc gcagccgaac gaccgagcgc
     6841 agcgagtcag tgagcgagga agcggaagag cgcccaatac gcaaaccgcc tctccccgcg
     6901 cgttggccga ttcattaatg cagggggat
//
