LOCUS       sequence-39515-e        6981 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6981)
  AUTHORS   Kobayashi T, Antar AA, Boehme KW, Danthi P, Eby EA, Guglielmi KM, 
            Holm GH, Johnson EM, Maginnis MS, Naik S, Skelton WB, Wetzel JD, 
            Wilson GJ, Chappell JD, Dermody TS
  TITLE     A plasmid-based reverse genetics system for animal double-stranded 
            RNA viruses.
  JOURNAL   Cell Host Microbe. 2007 Apr 19;1(2):147-57.
  PUBMED    18005692
REFERENCE   2  (bases 1 to 6981)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell Host 
            Microbe."; date: "2007-04-19"; volume: "1(2)"; pages: "147-57"
COMMENT     Sequence Label: pT7-L3T1L
FEATURES             Location/Qualifiers
     source          1..6981
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(4082..4100)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      4096..4143
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     promoter        complement(4211..4229)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(4212..4229)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(4520..4539)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     primer_bind     complement(4750..4769)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     4869..4891
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(4929..4947)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        5015..5119
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5120..5188,5189..5980)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5338..5357)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      6151..6739
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6640..6659
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     6893..6910
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 atcgatcccg ggaattctaa tacgactcac tatagctaat cgtcaggatg aagcggattc
       61 caaggaagac aaagggcaaa tccagcggaa agggcaatga ctcaacagaa agatcggacg
      121 atggctcgag ccaattacga gacaagcaaa acaataaggc tggccctgcc actacggagc
      181 ctggcacatc taaccgagag caatacagag cccgaccagg tattgcatct gtgcagaggg
      241 ccactgaaag tgcagaactg cccatgaaga ataatgacga agggacgcca gataagaaag
      301 gaaatactag gggcgactta gttaatgagc atagtgaggc taaagacgag gcggatgaag
      361 cgacgcagaa gcaggcaaaa gacacagaca aaagtaaagc gcaagtcaca tattcagaca
      421 ctggtatcaa taatgctaat gaactgtcaa gatctgggaa tgtggataat gagggtggaa
      481 gtaatcagaa gccgatgtcc accagaatag ctgaagcaac gtctactata gtgtcgaaac
      541 atcctgcgcg tgttgggtta ccacctaccg ctagcagtgg tcatgggtat cagtgccacg
      601 tctgttctgc agtcctgttt agtcctttag acctagatgc ccacgtcgcc tcacatggtt
      661 tgcatggtaa catgacatta acatcgagtg agattcagcg acatataact gagttcatca
      721 gctcatggca aaatcatcct attgttcaag tttcggctga tgtcgaaaat aagaagactg
      781 ctcaactgct tcacgctgac actcctcgac tcgtcacttg ggatgctggt ttgtgtactt
      841 cgttcaaaat cgtcccgatt gtgccagctc aggtgccgca ggatgtactg gcctatacgt
      901 ttttcacctc ttcatatgcc atccaatcac cgtttccaga ggcggcagtg tctaggattg
      961 tggtgcatac gagatgggca tctaatgttg actttgaccg agattcgtct gtcatcatgg
     1021 cgccacctac agaaaacaat atccatttgt ttaagcagtt actaaatact gaaaccctgt
     1081 ctgtaagggg ggctaatccg ctaatgttta gagcgaacgt gttgcatatg ttgctggagt
     1141 tcgtattaga taacttgtat ctgaacagac atacgggatt ctctcaagac cacacaccat
     1201 ttactgaggg tgctaatctg cgctcacttc ctggccccga tgctgagaaa tggtactcga
     1261 ttatgtatcc aacgcgcatg ggaacgccga atgtatcaaa aatatgtaat ttcgtcgcct
     1321 cttgtgtgcg aaatcgggtt ggacgatttg atcgagcaca gatgatgaac ggagctatgt
     1381 cagagtgggt ggatgtcttc gagacttcag acgcgcttac cgtctccatt cgaggtcgat
     1441 ggatggctag actagctcgc atgaacataa atccaacaga gatcgaatgg gcgttgactg
     1501 aatgtgcaca aggatatgtg actgtcacaa gtccttacgc tcctagcgta aatagattga
     1561 tgccctatcg tatctccaac gctgagcggc aaatatcaca gataatcagg atcatgaaca
     1621 ttggcaataa cgcgacggtg atacaacctg ttctgcaaga tatttcagta ctccttcaac
     1681 gcatatcacc actccaaata gatccaacta ttatttccaa cactatgtca acagtctcgg
     1741 agtctactac tcagaccctc agccccgcgt cctcaatttt gggtaaacta cgaccgagta
     1801 actcagattt ctctagtttt agagtcgcgt tggctggatg gctttataat ggagttgtga
     1861 cgacggtgat tgatgatagt tcatatccaa aagacggtgg cagcgtgacc tcacttgaaa
     1921 atctgtggga tttcttcatc cttgcgcttg ctctaccact gacaactgac ccctgtgcac
     1981 ctgtgaaagc attcatgacc ttagctaaca tgatggttgg tttcgagaca atccctatgg
     2041 ataatcagat ctatactcaa tcgagacgcg cgagtgcttt ctcaacgcct cacacgtggc
     2101 cacgatgctt catgaatatc cagttaattt ctccaatcga tgctcctatc ttgcgacagt
     2161 gggctgaaat tattcataga tactggccta acccttcaca gattcgttat ggtgcaccga
     2221 acgttttcgg ctcggcaaat ttgttcactc cacctgaggt gctgttattg ccaatcgatc
     2281 atcaaccagc taatgtaaca acgccaacgc tggacttcac caatgagttg actaattggc
     2341 gcgctcgtgt ctgtgagctt atgaagaatc ttgttgataa tcaaagatat caacctggat
     2401 ggacacaaag tctagtctcg tcaatgcgcg gaacgctaga caaattgaag ttgattaaat
     2461 cgatgacacc catgtatctg caacagctgg ctccggtaga gttagcagtg atagctccca
     2521 tgttgccttt cccacctttc caggtgccct acgtccgtct cgatcgtgat agagttccaa
     2581 caatggtcgg agtaacacga cagtcacgag atactattac tcagccagcg ctatcgctgt
     2641 cgacgaccaa tactactgtt ggcgtgccac tagctctaga cgcgagggct atcaccgttg
     2701 cgctgttgtc agggaaatat ccgccggatt tagtgacaaa tgtatggtac gctgatgcca
     2761 tttatccaat gtatgcagac actgaggtgt tctctaatct tcagagagac atgattacct
     2821 gcgaggccgt gcagacatta gtgactctgg tggcgcaaat atcagagacc cagtatcctg
     2881 tagataggta tcttgattgg atcccatcac tgagagcatc ggcggcgacg gcggcgacat
     2941 ttgctgagtg ggttaatact tcaatgaaga cggcgtttga tttgtctgac atgctgttag
     3001 agcctctcct aagcggtgat ccgaggatga ctcaactagc gattcagtat cagcaataca
     3061 atggcagaac gtttaatgtc atacctgaaa tgccaggttc agttattgct gactgcgttc
     3121 aactaacagc agaagtcttc aatcacgaat ataacctgtt tgggattgcg cggggtgata
     3181 tcatcattgg ccgtgttcag tcgacacatt tgtggtcacc gctggctcct ccacctgacc
     3241 tggtgtttga tcgtgacact cctggtgttc acatcttcgg acgagattgc cgcatatcgt
     3301 ttggaatgaa tggcgccgcg ccaatgatta gagatgagac tggaatgatg gtgccttttg
     3361 aaggaaattg gatcttccca ctggcgcttt ggcaaatgaa tacacgatat tttaatcaac
     3421 aattcgacgc gtggattaag acaggagagt tgcgaatccg tattgagatg ggcgcgtatc
     3481 catatatgtt gcattactat gatccacgtc agtacgctaa tgcatggaat ttaacatccg
     3541 cctggcttga agaaattaca ccgacgagca tcccatccgt gcctttcatg gtgcccattt
     3601 caagtgatca tgacatttcc tctgccccag ctgtccaata catcatctcg actgaatata
     3661 atgatcggtc tttattctgc actaactcat catctcccca aactatcgct ggaccagaca
     3721 aacacattcc agttgaaaga tataacattc tgaccaaccc cgacgctcca cccacgcaga
     3781 tacaactgcc tgaagttgtt gacttgtata acgtcgtcac acgctatgcg tatgagactc
     3841 cgcctattac cgctgtcgtt atgggtgttc cttgatcctc atcctcccaa caggtgctag
     3901 agcatcgcgc tcgatgctag ttgggccgat tcatcgggtc ggcatggcat ctccacctcc
     3961 tcgcggtccg acctgggcat ccgaaggagg acgtcgtcca ctcggatggc taagggagag
     4021 ctcaaaaaaa aggatccggc tgctaacaaa gcccgaaagg aagctgagtt ggctgctgcc
     4081 accgctgagc aataactagc ataacccctt ggggcctcta aacgggtctt gaggggtttt
     4141 ttgctgaaag gaggaactat atccggatcg agatcctcta gagtcgacct gcaggcatgc
     4201 aagcttgtat tctatagtgt cacctaaatc gtatgtgtat gatacataag gttatgtatt
     4261 aattgtagcc gcgttctaac gacaatatgt acaagcctaa ttgtgtagca tctggcttac
     4321 tgaagcagac cctatcatct ctctcgtaaa ctgccgtcag agtcggtttg gttggacgaa
     4381 ccttctgagt ttctggtaac gccgttccgc accccggaaa tggtcagcga accaatcagc
     4441 agggtcatcg ctagccagat cctctacgcc ggacgcatcg tggccggcat caccggcgcc
     4501 acaggtgcgg ttgctggcgc ctatatcgcc gacatcaccg atggggaaga tcgggctcgc
     4561 cacttcgggc tcatgagcgc ttgtttcggc gtgggtatgg tggcaggccc cgtggccggg
     4621 ggactgttgg gcgccatctc cttgcaccat tccttgcggc ggcggtgctc aacggcctca
     4681 acctactact gggctgcttc ctaatgcagg agtcgcataa gggagagcgt cgatatggtg
     4741 cactctcagt acaatctgct ctgatgccgc atagttaagc cagccccgac acccgccaac
     4801 acccgctgac gcgccctgac gggcttgtct gctcccggca tccgcttaca gacaagctgt
     4861 gaccgtctcc gggagctgca tgtgtcagag gttttcaccg tcatcaccga aacgcgcgag
     4921 acgaaagggc ctcgtgatac gcctattttt ataggttaat gtcatgataa taatggtttc
     4981 ttagacgtca ggtggcactt ttcggggaaa tgtgcgcgga acccctattt gtttattttt
     5041 ctaaatacat tcaaatatgt atccgctcat gagacaataa ccctgataaa tgcttcaata
     5101 atattgaaaa aggaagagta tgagtattca acatttccgt gtcgccctta ttcccttttt
     5161 tgcggcattt tgccttcctg tttttgctca cccagaaacg ctggtgaaag taaaagatgc
     5221 tgaagatcag ttgggtgcac gagtgggtta catcgaactg gatctcaaca gcggtaagat
     5281 ccttgagagt tttcgccccg aagaacgttt tccaatgatg agcactttta aagttctgct
     5341 atgtggcgcg gtattatccc gtattgacgc cgggcaagag caactcggtc gccgcataca
     5401 ctattctcag aatgacttgg ttgagtactc accagtcaca gaaaagcatc ttacggatgg
     5461 catgacagta agagaattat gcagtgctgc cataaccatg agtgataaca ctgcggccaa
     5521 cttacttctg acaacgatcg gaggaccgaa ggagctaacc gcttttttgc acaacatggg
     5581 ggatcatgta actcgccttg atcgttggga accggagctg aatgaagcca taccaaacga
     5641 cgagcgtgac accacgatgc ctgtagcaat ggcaacaacg ttgcgcaaac tattaactgg
     5701 cgaactactt actctagctt cccggcaaca attaatagac tggatggagg cggataaagt
     5761 tgcaggacca cttctgcgct cggcccttcc ggctggctgg tttattgctg ataaatctgg
     5821 agccggtgag cgtgggtctc gcggtatcat tgcagcactg gggccagatg gtaagccctc
     5881 ccgtatcgta gttatctaca cgacggggag tcaggcaact atggatgaac gaaatagaca
     5941 gatcgctgag ataggtgcct cactgattaa gcattggtaa ctgtcagacc aagtttactc
     6001 atatatactt tagattgatt taaaacttca tttttaattt aaaaggatct aggtgaagat
     6061 cctttttgat aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc
     6121 agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg
     6181 ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct
     6241 accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa atactgtcct
     6301 tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct
     6361 cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg
     6421 gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc
     6481 gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga
     6541 gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg
     6601 cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta
     6661 tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg
     6721 ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg
     6781 ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtat
     6841 taccgccttt gagtgagctg ataccgctcg ccgcagccga acgaccgagc gcagcgagtc
     6901 agtgagcgag gaagcggaag agcgcccaat acgcaaaccg cctctccccg cgcgttggcc
     6961 gattcattaa tgcaggggga t
//
