LOCUS       sequence-395159-        6142 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Express deltaLight biosensor in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6142)
  AUTHORS   Dong C, Gowrishankar R, Jin Y, He XJ, Gupta A, Wang H, Sayar-Atasoy 
            N, Flores RJ, Mahe K, Tjahjono N, Liang R, Marley A, Or Mizuno G, Lo
            DK, Sun Q, Whistler JL, Li B, Gomes I, Von Zastrow M, Tejeda HA, 
            Atasoy D, Devi LA, Bruchas MR, Banghart MR, Tian L
  TITLE     Unlocking opioid neuropeptide dynamics with genetically encoded 
            biosensors.
  JOURNAL   Nat Neurosci. 2024 Jul 15. doi: 10.1038/s41593-024-01697-1.
  PUBMED    39009835
REFERENCE   2  (bases 1 to 6142)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Neurosci. 2024 Jul 15. doi: 10.1038/s41593-024-01697-1."
COMMENT     Sequence Label: pCMV- delta-Light
FEATURES             Location/Qualifiers
     source          1..6142
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        166..469
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        470..673
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     624..644
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     1780..1801
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     complement(2168..2187)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     polyA_signal    3035..3156
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3072..3091)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     3126..3145
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      complement(3163..3618)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3300..3321)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     3512..3531
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     promoter        3645..3749
                     /gene="bla"
                     /label=AmpR promoter
     promoter        3751..4108
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     enhancer        3751..3942
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(3774..3794)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      3959..4094
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             4143..4937
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             4143..4937
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(4197..4216)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     4807..4826
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     complement(5125..5144)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    5169..5216
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     rep_origin      5545..6133
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6034..6053
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 aacgcggcct ttttacggtt cctggccttt tgctggcctt ttgctcacat gttctttcct
       61 gcgttatccc ctgattctgt ggataaccgt attaccgcca tgcattagtt attaatagta
      121 atcaattacg gggtcattag ttcatagccc atatatggag ttccgcgtta cataacttac
      181 ggtaaatggc ccgcctggct gaccgcccaa cgacccccgc ccattgacgt caataatgac
      241 gtatgttccc atagtaacgc caatagggac tttccattga cgtcaatggg tggagtattt
      301 acggtaaact gcccacttgg cagtacatca agtgtatcat atgccaagta cgccccctat
      361 tgacgtcaat gacggtaaat ggcccgcctg gcattatgcc cagtacatga ccttatggga
      421 ctttcctact tggcagtaca tctacgtatt agtcatcgct attaccatgg tgatgcggtt
      481 ttggcagtac atcaatgggc gtggatagcg gtttgactca cggggatttc caagtctcca
      541 ccccattgac gtcaatggga gtttgttttg gcaccaaaat caacgggact ttccaaaatg
      601 tcgtaacaac tccgccccat tgacgcaaat gggcggtagg cgtgtacggt gggaggtcta
      661 tataagcaga gctggtttag tgaaccgtca gatccgctag cgctaccgga ctcagatctc
      721 gagcttaagc ttaccggtgt gtaacatgaa gacgatcatc gccctgagct acatcttctg
      781 cctggtgttc gccgactaca aggacgatga tgacgccatg gaaccagccc ctagtgcagg
      841 cgcagaattg cagccccctc tgtttgccaa cgcaagtgac gcctaccctt ccgcgtgccc
      901 cagcgcaggg gctaacgcca gcggccctcc gggggcaaga agcgctagca gtctggccct
      961 ggctattgcc attaccgccc tgtattccgc cgtgtgtgca gtgggacttc tgggtaacgt
     1021 gctggtgatg ttcgggattg tgcgctacac caagatgaaa accgcaacca acatttacat
     1081 ttttaacctg gctttggccg acgcactggc caccagtacc ctgccattcc aaagcgccaa
     1141 atacctgatg gaaacgtggc cgtttgggga gttgctgtgt aaggccgtgc tgagcattga
     1201 ttactacaac atgtttacct ccatctttac tctgacaatg atgagtgtgg atcggtatat
     1261 tgcagtctgc cacccaaaga aggccctgga tttccggact cccgcaaagg ccaaactgat
     1321 aaatatatgt atctgggtcc tggcctcagg cgtgggagta cccattatgg tgatggctgt
     1381 gacacggccc cgggatgggg ccgttgtctg catgcttcaa tttcccagcc cctcctggta
     1441 ctgggatacc gtcactaaga tctgtgtctt cctgtttgcg tttgtggtac caatcctgat
     1501 cataacggtc tgctacgggc tgatgttgct gaggctgagg agtgtgaggc tgctgagcct
     1561 gagctcaggc attaacgtct atatcaaggc cgacaagcag aagaacggca tcaaggcgaa
     1621 cttcaagatc cgccacaaca tcgaggacgg cggcgtgcag ctcgcctacc actaccagca
     1681 gaacaccccc atcggcgacg gccccgtgct gctgcccgac aaccactacc tgagcgtgca
     1741 gtccaaactt tcgaaagacc ccaacgagaa gcgcgatcac atggtcctgc tggagttcgt
     1801 gaccgccgcc gggatcactc tcggcatgga cgagctgtac aagggcggta ccggagggag
     1861 catggtgagc aagggcgagg agctgttcac cggggtggtg cccatcctgg tcgagttgga
     1921 cggcgacgta aacggccaca agttcagcgt gtccggcgag ggtgagggcg atgccaccta
     1981 cggcaagctg accctgaagt tcatctgcac caccggcaag ctgcccgtgc cctggcccac
     2041 cctcgtgacc accctgacct acggcgtgca gtgcttcagc cgctaccccg accacatgaa
     2101 gcagcacgac ttcttcaagt ccgccatgcc cgaaggctac atccaggagc gcaccatctt
     2161 cttcaaggac gacggcaact acaagacccg cgccgaggtg aagttcgagg gcgacaccct
     2221 ggtgaaccgc atcgagctga agggcatcga cttcaaggag gacggcaaca tcctggggca
     2281 caagctggag tacaacccac atgaccaact gaaagaaaaa gatcgaagtc tgcgccggat
     2341 tacacggatg gttctggtag tggtgggcgc gttcgtggtg tgttgggccc ccatccacat
     2401 attcgtgatt gtgtggacat tggtcgacat tgaccgccga gatcctctgg tggtagctgc
     2461 tctccatctg tgcatcgcct tgggctatgc caatagttct ctgaatccgg tgctgtatgc
     2521 cttcctggac gagaacttca agagatgttt tcggcagctg tgtaggaagc cttgtgggcg
     2581 acccgaccct tcaagcttca gccgagctcg cgaagcaaca gcaagagaga gggtaaccgc
     2641 ctgtactccc agcgatgggc ccgggggtgg agccgccgcg cagtcccagc ccatcctcaa
     2701 caccaaggag atggccccgc agagcaagcc tccagaggag ctggagatga gcagcatgcc
     2761 cagccccgtg gcccctctgc ccgcacgcac ggagggcgtc atcgacatgc ggagcatgtc
     2821 cagcattgac agcttcatca gctgtgccac ggacttccct gaagccacca gattcttctg
     2881 ctacgagaac gaggtgtgat aatataatat atagcggccg cgactctaga tcataatcag
     2941 ccataccaca tttgtagagg ttttacttgc tttaaaaaac ctcccacacc tccccctgaa
     3001 cctgaaacat aaaatgaatg caattgttgt tgttaacttg tttattgcag cttataatgg
     3061 ttacaaataa agcaatagca tcacaaattt cacaaataaa gcattttttt cactgcattc
     3121 tagttgtggt ttgtccaaac tcatcaatgt atcttaaggc gtaaattgta agcgttaata
     3181 ttttgttaaa attcgcgtta aatttttgtt aaatcagctc attttttaac caataggccg
     3241 aaatcggcaa aatcccttat aaatcaaaag aatagaccga gatagggttg agtgttgttc
     3301 cagtttggaa caagagtcca ctattaaaga acgtggactc caacgtcaaa gggcgaaaaa
     3361 ccgtctatca gggcgatggc ccactacgtg aaccatcacc ctaatcaagt tttttggggt
     3421 cgaggtgccg taaagcacta aatcggaacc ctaaagggag cccccgattt agagcttgac
     3481 ggggaaagcc ggcgaacgtg gcgagaaagg aagggaagaa agcgaaagga gcgggcgcta
     3541 gggcgctggc aagtgtagcg gtcacgctgc gcgtaaccac cacacccgcc gcgcttaatg
     3601 cgccgctaca gggcgcgtca ggtggcactt ttcggggaaa tgtgcgcgga acccctattt
     3661 gtttattttt ctaaatacat tcaaatatgt atccgctcat gagacaataa ccctgataaa
     3721 tgcttcaata atattgaaaa aggaagagtc ctgaggcgga aagaaccagc tgtggaatgt
     3781 gtgtcagtta gggtgtggaa agtccccagg ctccccagca ggcagaagta tgcaaagcat
     3841 gcatctcaat tagtcagcaa ccaggtgtgg aaagtcccca ggctccccag caggcagaag
     3901 tatgcaaagc atgcatctca attagtcagc aaccatagtc ccgcccctaa ctccgcccat
     3961 cccgccccta actccgccca gttccgccca ttctccgccc catggctgac taattttttt
     4021 tatttatgca gaggccgagg ccgcctcggc ctctgagcta ttccagaagt agtgaggagg
     4081 cttttttgga ggcctaggct tttgcaaaga tcgatcaaga gacaggatga ggatcgtttc
     4141 gcatgattga acaagatgga ttgcacgcag gttctccggc cgcttgggtg gagaggctat
     4201 tcggctatga ctgggcacaa cagacaatcg gctgctctga tgccgccgtg ttccggctgt
     4261 cagcgcaggg gcgcccggtt ctttttgtca agaccgacct gtccggtgcc ctgaatgaac
     4321 tgcaagacga ggcagcgcgg ctatcgtggc tggccacgac gggcgttcct tgcgcagctg
     4381 tgctcgacgt tgtcactgaa gcgggaaggg actggctgct attgggcgaa gtgccggggc
     4441 aggatctcct gtcatctcac cttgctcctg ccgagaaagt atccatcatg gctgatgcaa
     4501 tgcggcggct gcatacgctt gatccggcta cctgcccatt cgaccaccaa gcgaaacatc
     4561 gcatcgagcg agcacgtact cggatggaag ccggtcttgt cgatcaggat gatctggacg
     4621 aagagcatca ggggctcgcg ccagccgaac tgttcgccag gctcaaggcg agcatgcccg
     4681 acggcgagga tctcgtcgtg acccatggcg atgcctgctt gccgaatatc atggtggaaa
     4741 atggccgctt ttctggattc atcgactgtg gccggctggg tgtggcggac cgctatcagg
     4801 acatagcgtt ggctacccgt gatattgctg aagagcttgg cggcgaatgg gctgaccgct
     4861 tcctcgtgct ttacggtatc gccgctcccg attcgcagcg catcgccttc tatcgccttc
     4921 ttgacgagtt cttctgagcg ggactctggg gttcgaaatg accgaccaag cgacgcccaa
     4981 cctgccatca cgagatttcg attccaccgc cgccttctat gaaaggttgg gcttcggaat
     5041 cgttttccgg gacgccggct ggatgatcct ccagcgcggg gatctcatgc tggagttctt
     5101 cgcccaccct agggggaggc taactgaaac acggaaggag acaataccgg aaggaacccg
     5161 cgctatgacg gcaataaaaa gacagaataa aacgcacggt gttgggtcgt ttgttcataa
     5221 acgcggggtt cggtcccagg gctggcactc tgtcgatacc ccaccgagac cccattgggg
     5281 ccaatacgcc cgcgtttctt ccttttcccc accccacccc ccaagttcgg gtgaaggccc
     5341 agggctcgca gccaacgtcg gggcggcagg ccctgccata gcctcaggtt actcatatat
     5401 actttagatt gatttaaaac ttcattttta atttaaaagg atctaggtga agatcctttt
     5461 tgataatctc atgaccaaaa tcccttaacg tgagttttcg ttccactgag cgtcagaccc
     5521 cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt
     5581 gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac
     5641 tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg ttcttctagt
     5701 gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct
     5761 gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga
     5821 ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac
     5881 acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagctatg
     5941 agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa gcggcagggt
     6001 cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc
     6061 tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt caggggggcg
     6121 gagcctatgg aaaaacgcca gc
//
