LOCUS       sequence-39516-e        5379 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5379)
  AUTHORS   Kobayashi T, Antar AA, Boehme KW, Danthi P, Eby EA, Guglielmi KM, 
            Holm GH, Johnson EM, Maginnis MS, Naik S, Skelton WB, Wetzel JD, 
            Wilson GJ, Chappell JD, Dermody TS
  TITLE     A plasmid-based reverse genetics system for animal double-stranded 
            RNA viruses.
  JOURNAL   Cell Host Microbe. 2007 Apr 19;1(2):147-57.
  PUBMED    18005692
REFERENCE   2  (bases 1 to 5379)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell Host 
            Microbe."; date: "2007-04-19"; volume: "1(2)"; pages: "147-57"
COMMENT     Sequence Label: pT7-M1T1L
FEATURES             Location/Qualifiers
     source          1..5379
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             1130..1141
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(2480..2498)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      2494..2541
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     promoter        complement(2609..2627)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(2610..2627)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(2918..2937)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     primer_bind     complement(3148..3167)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     3267..3289
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(3327..3345)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        3413..3517
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(3518..3586,3587..4378)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(3736..3755)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      4549..5137
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5038..5057
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     5291..5308
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 atcgatcccg ggtaatacga ctcactatag ctattcgcgg tcatggctta catcgcagtt
       61 cctgcggtgg tggattcacg ttcaagtgag gctattggac tgctagaatc gtttggagta
      121 gacgctgggg ctgatgcgaa tgacgtttca tatcaagatc atgactatgt gttggatcag
      181 ttacagtata tgttagatgg atatgaggct ggcgacgtta tcgatgcact cgtccacaag
      241 aattggttac atcactccgt ctattgcttg ttgccaccca aaagtcaact actagagtat
      301 tggaaaagta atccttcagt gataccggac aacgttgatc gtcggcttcg taaacgacta
      361 atgctaaaga aagatctcag aaaagatgat gaatacaatc aactagcgcg tgctttcaag
      421 atatcggatg tctacgcacc tctcatctca tccacgacgt caccgatgac aatgatccag
      481 aacttgaatc aaggcgagat cgtgtacacc acgacggaca gggtaattgg ggctagaatc
      541 ttgttatatg ctcctagaaa gtactatgcg tcaactctat catttactat gactaagtgc
      601 atcattccgt ttggcaaaga ggtgggtcgt gttcctcact ctagatttaa tgttggcaca
      661 tttccatcaa ttgctacccc gaaatgtttt gtcatgagtg gggttgatat tgagtccatc
      721 ccaaatgaat tcatcaagtt gttttaccag cgcgtcaaga gtgttcacgc caatatacta
      781 aatgacatat cacctcagat cgtctctgac atgataaaca gaaagcgttt gcgcgttcat
      841 actccatcag atcgtcgagc cgcgcagttg atgcatttgc cctaccatgt taaacgagga
      901 gcgtctcacg tcgacgttta caaggtggat gttgtagacg tgttgtttga ggtagtggat
      961 gtggccgatg ggttgcgcaa cgtatctagg aaactaacta tgcataccgt tccggtatgt
     1021 attcttgaaa tgttgggtat tgagattgcg gactattgca ttcgtcaaga ggatggaatg
     1081 ttcacagatt ggttcctact tttaaccatg ctatctgatg gcttaactga tagaaggacg
     1141 cattgtcaat acttgattaa tccgtcaagt gtgcctcctg atgtgatact taacatctca
     1201 attactggat ttataaatag gcatacaatc gatgtcatgc ctgatatata tgacttcgtt
     1261 aaacccattg gcgctgtgct gcctaaggga tcatttaaat caacaattat gagagttctt
     1321 gattcaatat caatattagg agtccagatc atgccgcgcg cgcatgtagt tgactcggat
     1381 gaggtgggcg agcaaatgga gcctacgttt gagcatgcgg ttatggagat atacaaaggg
     1441 attgctggcg ttgactcgct ggatgatctc atcaagtggg tgctgaactc ggatctcatt
     1501 ccgcatgatg acaggcttgg ccaattattt caagcgtttc tgcctctcgc aaaggacttg
     1561 ttagctccaa tggccagaaa gttttatgat aactcaatga gtgagggtag attgctgaca
     1621 ttcgctcatg ccgacagtga gttgctgaac gcaaattact ttggtcattt attgcgacta
     1681 aaaataccat atattacaga ggttaatctg atgattcgca agaatcgtga gggtggagag
     1741 ctatttcagc ttgtgttatc gtatctatat aaaatgtatg ctactagcgc gcagcctaaa
     1801 tggtttggat cattattgcg attgttaata tgtccctggt tacatatgga gaaattaata
     1861 ggagaagcag acccggcatc tacgtcggct gaaattggat ggcatatccc tcgtgaacag
     1921 ctgatgcaag atggatggtg tggatgtgaa gatggattca ttccctatgt tagcatacgt
     1981 gcgccaagac tggttatgga ggagttgatg gagaagaact ggggccaata tcatgcccaa
     2041 gttattgtca ctgatcagct tgtcgtaggc gaaccgcgga gggtatctgc caaggctgtg
     2101 atcaagggta atcacttacc agttaagtta gtttcacgat ttgcatgttt cacattgacg
     2161 gcgaagtatg agatgaggct ctcgtgcggc catagcactg gacggggggc tgcatacaat
     2221 gcgagactag ctttccgatc tgacttggcg tgatccgtga catgcgtagt gtgacacctg
     2281 cccctaggtc aatgggggta gggggcgggc taagactacg tacgcgcttc atcgggtcgg
     2341 catggcatct ccacctcctc gcggtccgac ctgggcatcc gaaggaggac gtcgtccact
     2401 cggatggcta agggagagct caaaaaaaag gatccggctg ctaacaaagc ccgaaaggaa
     2461 gctgagttgg ctgctgccac cgctgagcaa taactagcat aaccccttgg ggcctctaaa
     2521 cgggtcttga ggggtttttt gctgaaagga ggaactatat ccggatcgag atcctctaga
     2581 gtcgacctgc aggcatgcaa gcttgtattc tatagtgtca cctaaatcgt atgtgtatga
     2641 tacataaggt tatgtattaa ttgtagccgc gttctaacga caatatgtac aagcctaatt
     2701 gtgtagcatc tggcttactg aagcagaccc tatcatctct ctcgtaaact gccgtcagag
     2761 tcggtttggt tggacgaacc ttctgagttt ctggtaacgc cgttccgcac cccggaaatg
     2821 gtcagcgaac caatcagcag ggtcatcgct agccagatcc tctacgccgg acgcatcgtg
     2881 gccggcatca ccggcgccac aggtgcggtt gctggcgcct atatcgccga catcaccgat
     2941 ggggaagatc gggctcgcca cttcgggctc atgagcgctt gtttcggcgt gggtatggtg
     3001 gcaggccccg tggccggggg actgttgggc gccatctcct tgcaccattc cttgcggcgg
     3061 cggtgctcaa cggcctcaac ctactactgg gctgcttcct aatgcaggag tcgcataagg
     3121 gagagcgtcg atatggtgca ctctcagtac aatctgctct gatgccgcat agttaagcca
     3181 gccccgacac ccgccaacac ccgctgacgc gccctgacgg gcttgtctgc tcccggcatc
     3241 cgcttacaga caagctgtga ccgtctccgg gagctgcatg tgtcagaggt tttcaccgtc
     3301 atcaccgaaa cgcgcgagac gaaagggcct cgtgatacgc ctatttttat aggttaatgt
     3361 catgataata atggtttctt agacgtcagg tggcactttt cggggaaatg tgcgcggaac
     3421 ccctatttgt ttatttttct aaatacattc aaatatgtat ccgctcatga gacaataacc
     3481 ctgataaatg cttcaataat attgaaaaag gaagagtatg agtattcaac atttccgtgt
     3541 cgcccttatt cccttttttg cggcattttg ccttcctgtt tttgctcacc cagaaacgct
     3601 ggtgaaagta aaagatgctg aagatcagtt gggtgcacga gtgggttaca tcgaactgga
     3661 tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc caatgatgag
     3721 cacttttaaa gttctgctat gtggcgcggt attatcccgt attgacgccg ggcaagagca
     3781 actcggtcgc cgcatacact attctcagaa tgacttggtt gagtactcac cagtcacaga
     3841 aaagcatctt acggatggca tgacagtaag agaattatgc agtgctgcca taaccatgag
     3901 tgataacact gcggccaact tacttctgac aacgatcgga ggaccgaagg agctaaccgc
     3961 ttttttgcac aacatggggg atcatgtaac tcgccttgat cgttgggaac cggagctgaa
     4021 tgaagccata ccaaacgacg agcgtgacac cacgatgcct gtagcaatgg caacaacgtt
     4081 gcgcaaacta ttaactggcg aactacttac tctagcttcc cggcaacaat taatagactg
     4141 gatggaggcg gataaagttg caggaccact tctgcgctcg gcccttccgg ctggctggtt
     4201 tattgctgat aaatctggag ccggtgagcg tgggtctcgc ggtatcattg cagcactggg
     4261 gccagatggt aagccctccc gtatcgtagt tatctacacg acggggagtc aggcaactat
     4321 ggatgaacga aatagacaga tcgctgagat aggtgcctca ctgattaagc attggtaact
     4381 gtcagaccaa gtttactcat atatacttta gattgattta aaacttcatt tttaatttaa
     4441 aaggatctag gtgaagatcc tttttgataa tctcatgacc aaaatccctt aacgtgagtt
     4501 ttcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt gagatccttt
     4561 ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg
     4621 tttgccggat caagagctac caactctttt tccgaaggta actggcttca gcagagcgca
     4681 gataccaaat actgtccttc tagtgtagcc gtagttaggc caccacttca agaactctgt
     4741 agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg ccagtggcga
     4801 taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg cgcagcggtc
     4861 gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct acaccgaact
     4921 gagataccta cagcgtgagc tatgagaaag cgccacgctt cccgaaggga gaaaggcgga
     4981 caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc ttccaggggg
     5041 aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt
     5101 tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg cggccttttt
     5161 acggttcctg gccttttgct ggccttttgc tcacatgttc tttcctgcgt tatcccctga
     5221 ttctgtggat aaccgtatta ccgcctttga gtgagctgat accgctcgcc gcagccgaac
     5281 gaccgagcgc agcgagtcag tgagcgagga agcggaagag cgcccaatac gcaaaccgcc
     5341 tctccccgcg cgttggccga ttcattaatg cagggggat
//
