LOCUS       sequence-395171-        8982 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Bacterial protein expression of Dhh1.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8982)
  AUTHORS   Currie SL, Xing W, Muhlrad D, Decker CJ, Parker R, Rosen MK
  TITLE     Quantitative reconstitution of yeast RNA processing bodies.
  JOURNAL   Proc Natl Acad Sci U S A. 2023 Apr 4;120(14):e2214064120. doi: 
            10.1073/pnas.2214064120. Epub 2023 Mar 27.
  PUBMED    36972455
REFERENCE   2  (bases 1 to 8982)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A."; date: "2023-04-4"; volume: "120(14):e2214064120. 
            doi"; pages: " 10.1073/pnas.2214064120. Epub 2023 Mar 27"
COMMENT     Sequence Label: Dhh1-EGFP
FEATURES             Location/Qualifiers
     source          1..8982
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        join(78..83,84..99,100..106)
                     /label=tac promoter
                     /note="strong E. coli promoter; hybrid between the trp and 
                     lac UV5 promoters"
     protein_bind    114..130
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     119..141
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     CDS             200..1300
                     /codon_start=1
                     /gene="malE (mutated)"
                     /product="maltose binding protein from E. coli"
                     /label=MBP
                     /note="This version of the gene does not encode a signal 
                     sequence, so MBP will remain in the cytosol."
                     /translation="MKTEEGKLVIWINGDKGYNGLAEVGKKFEKDTGIKVTVEHPDKLE
                     EKFPQVAATGDGPDIIFWAHDRFGGYAQSGLLAEITPDKAFQDKLYPFTWDAVRYNGKL
                     IAYPIAVEALSLIYNKDLLPNPPKTWEEIPALDKELKAKGKSALMFNLQEPYFTWPLIA
                     ADGGYAFKYENGKYDIKDVGVDNAGAKAGLTFLVDLIKNKHMNADTDYSIAEAAFNKGE
                     TAMTINGPWAWSNIDTSKVNYGVTVLPTFKGQPSKPFVGVLSAGINAASPNKELAKEFL
                     ENYLLTDEGLEAVNKDKPLGAVALKSYEEELAKDPRIAATMENAQKGEIMPNIPQMSAF
                     WYAVRTAVINAASGRQTVDEALKDAQT"
     regulatory      1157..1166
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     1274..1297
                     /label=MBP-F
                     /note="Maltose binding protein, forward primer"
     CDS             1349..1360
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     CDS             1373..1393
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             join(2942..2944,2945..2947,2948..3658)
                     /codon_start=1
                     /product="brightly fluorescent monomeric variant of GFP"
                     /label=mEmerald
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHKVYITADKQKNGIK
                     VNFKTRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(2987..3008)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(3248..3267)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     3595..3616
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     CDS             3671..3691
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             3692..3709
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(3722..3739)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(3722..3738)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3731..3753)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     complement(3917..3934)
                     /label=pBAD Reverse
                     /note="For vectors with E. coli araBAD promoter, reverse 
                     primer"
     terminator      4087..4173
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      4265..4292
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     promoter        4311..4402
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(4403..4471,4472..5263)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(4621..4640)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      complement(5305..5818)
                     /direction=LEFT
                     /label=M13 ori
                     /note="M13 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5500..5521)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     5712..5731
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     rep_origin      5929..6517
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6418..6437
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     6671..6688
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(6794..6813)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     6954..6976
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     CDS             complement(6970..7161)
                     /codon_start=1
                     /gene="rop"
                     /product="Rop protein, which maintains plasmids at low copy
                     number"
                     /label=rop
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
     promoter        7657..7734
                     /gene="lacI (mutant)"
                     /label=lacIq promoter
                     /note="In the lacIq allele, a single base change in the 
                     promoter boosts expression of the lacI gene about 10-fold."
     CDS             7735..8817
                     /codon_start=1
                     /gene="lacI"
                     /product="lac repressor"
                     /label=lacI
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
                     /translation="MKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAEL
                     NYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERSGV
                     EACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNVPALFLDVSDQTPINSIIFSH
                     EDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREGDWSA
                     MSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDTEDSSC
                     YIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLAPNTQTASPR
                     ALADSLMQLARQVSRLESGQ"
     primer_bind     complement(7754..7773)
                     /label=LacI-R
                     /note="LacI, reverse primer"
ORIGIN
        1 aaggcgcact cccgttctgg ataatgtttt ttgcgccgac atcataacgg ttctggcaaa
       61 tattctgaaa tgagctgttg acaattaatc atcggctcgt ataatgtgtg gaattgtgag
      121 cggataacaa tttcacacag gaaacagcca gtccgtttag gtgttttcac gagcacttca
      181 ccaacaagga ccatagatta tgaaaactga agaaggtaaa ctggtaatct ggattaacgg
      241 cgataaaggc tataacggtc tcgctgaagt cggtaagaaa ttcgagaaag ataccggaat
      301 taaagtcacc gttgagcatc cggataaact ggaagagaaa ttcccacagg ttgcggcaac
      361 tggcgatggc cctgacatta tcttctgggc acacgaccgc tttggtggct acgctcaatc
      421 tggcctgttg gctgaaatca ccccggacaa agcgttccag gacaagctgt atccgtttac
      481 ctgggatgcc gtacgttaca acggcaagct gattgcttac ccgatcgctg ttgaagcgtt
      541 atcgctgatt tataacaaag atctgctgcc gaacccgcca aaaacctggg aagagatccc
      601 ggcgctggat aaagaactga aagcgaaagg taagagcgcg ctgatgttca acctgcaaga
      661 accgtacttc acctggccgc tgattgctgc tgacgggggt tatgcgttca agtatgaaaa
      721 cggcaagtac gacattaaag acgtgggcgt ggataacgct ggcgcgaaag cgggtctgac
      781 cttcctggtt gacctgatta aaaacaaaca catgaatgca gacaccgatt actccatcgc
      841 agaagctgcc tttaataaag gcgaaacagc gatgaccatc aacggcccgt gggcatggtc
      901 caacatcgac accagcaaag tgaattatgg tgtaacggta ctgccgacct tcaagggtca
      961 accatccaaa ccgttcgttg gcgtgctgag cgcaggtatt aacgccgcca gtccgaacaa
     1021 agagctggca aaagagttcc tcgaaaacta tctgctgact gatgaaggtc tggaagcggt
     1081 taataaagac aaaccgctgg gtgccgtagc gctgaagtct tacgaggaag agttggcgaa
     1141 agatccacgt attgccgcca ccatggaaaa cgcccagaaa ggtgaaatca tgccgaacat
     1201 cccgcagatg tccgctttct ggtatgccgt gcgtactgcg gtgatcaacg ccgccagcgg
     1261 tcgtcagact gtcgatgaag ccctgaaaga cgcgcagact aattcgagct cgaacaacaa
     1321 caacaataac aataacaaca acctcgggat cgagggaagg atttcagaat ttgaaaacct
     1381 gtattttcag ggccatatgg gttccatcaa taataacttc aacactaata ataacagtaa
     1441 tacggatctc gatcgggact ggaaaactgc attgaatatt cccaagaagg ataccagacc
     1501 acagacagat gatgtcttaa atacaaaggg taatactttt gaagattttt atttgaaaag
     1561 agagctgtta atgggtattt ttgaagccgg ttttgaaaaa ccatccccta ttcaagaaga
     1621 ggctattccg gtagcaataa ctggtagaga catattggcc agagcaaaga atggtacagg
     1681 taagacagcc gcatttgtta ttcctacgtt agagaaagtc aaaccaaagt tgaataaaat
     1741 tcaggctttg atcatggttc ccacaagaga gttagctctg caaacttccc aagtcgtccg
     1801 tacattggga aaacactgtg gtatttcatg tatggtgacc actggtggta ccaatttgag
     1861 agacgatatt ctaagattaa atgaaacagt ccatattttg gttggtactc ctggtagagt
     1921 gttggattta gcttcgagaa aagtagcaga tctgtctgat tgttcattat tcatcatgga
     1981 cgaagccgat aaaatgttat ctcgtgactt caagacaata attgaacaaa tcttatcctt
     2041 cttacctcca actcaccaat ctttattatt tagcgctact ttcccactaa cggttaagga
     2101 attcatggtt aaacatctgc ataagcctta tgaaattaat ttgatggaag aattgactct
     2161 aaagggtatt actcaatact acgcctttgt tgaagaaagg caaaagctac attgtttaaa
     2221 tactttattc tctaagcttc aaattaatca agccattatt ttttgtaatt ctactaaccg
     2281 tgtcgaatta ctagccaaga aaatcactga tttaggctat tcctgttatt actctcatgc
     2341 aagaatgaag caacaagaaa gaaataaagt tttccacgaa tttcgtcaag gtaaggttcg
     2401 tacattggtc tgctccgatt tattgacccg tggtatcgat attcaagccg ttaatgtcgt
     2461 tatcaatttc gatttcccca aaacggcaga aacatattta catcgtattg gtagatccgg
     2521 taggtttggc cacttgggtt tagcaatcaa tttgattaac tggaatgatc ggtttaacct
     2581 ttataaaatc gaacaagaac tgggcactga aattgcggct attccggcca ctatcgataa
     2641 gtcattgtat gtggctgaaa atgacgaaac ggtacctgtc ccattcccga tagagcaaca
     2701 gagctatcat caacaagcga tacctcagca acaattacca tctcagcaac aatttgctat
     2761 tcctccgcag caacatcatc cacaattcat ggttcctcct tcgcatcagc aacaacaggc
     2821 gtatcctcca ccgcaaatgc catcgcagca ggggtatcct ccacagcagg aacatttcat
     2881 ggcgatgcca cctggtcagt cacaacccca gtatggatcc gggtctggtt caggaatggc
     2941 catggtgagc aagggcgagg agctgttcac cggggtggtg cccatcctgg tcgagctgga
     3001 cggcgacgta aacggccaca agttcagcgt gtccggcgag ggcgagggcg atgccaccta
     3061 cggcaagctg accctgaagt tcatctgcac caccggcaag ctgcccgtgc cctggcccac
     3121 cctcgtgacc accttgacct acggcgtgca gtgcttcgcc cgctaccccg accacatgaa
     3181 gcagcacgac ttcttcaagt ccgccatgcc cgaaggctac gtccaggagc gcaccatctt
     3241 cttcaaggac gacggcaact acaagacccg cgccgaggtg aagttcgagg gcgacaccct
     3301 ggtgaaccgc atcgagctga agggcatcga cttcaaggag gacggcaaca tcctggggca
     3361 caagctggag tacaactaca acagccacaa ggtctatatc accgccgaca agcagaagaa
     3421 cggcatcaag gtgaacttca agacccgcca caacatcgag gacggcagcg tgcagctcgc
     3481 cgaccactac cagcagaaca cccccatcgg cgacggcccc gtgctgctgc ccgacaacca
     3541 ctacctgagc acccagtcca agctgagcaa agaccccaac gagaagcgcg atcacatggt
     3601 cctgctggag ttcgtgaccg ccgccgggat cactctcggc atggacgagc tgtacaaggg
     3661 aagcggccgc gaaaacctgt attttcaggg ccatcatcat catcatcact gaaagcttgg
     3721 cactggccgt cgttttacaa cgtcgtgact gggaaaaccc tggcgttacc caacttaatc
     3781 gccttgcagc acatccccct ttcgccagct ggcgtaatag cgaagaggcc cgcaccgatc
     3841 gcccttccca acagttgcgc agcctgaatg gcgaatggca gcttggctgt tttggcggat
     3901 gagataagat tttcagcctg atacagatta aatcagaacg cagaagcggt ctgataaaac
     3961 agaatttgcc tggcggcagt agcgcggtgg tcccacctga ccccatgccg aactcagaag
     4021 tgaaacgccg tagcgccgat ggtagtgtgg ggtctcccca tgcgagagta gggaactgcc
     4081 aggcatcaaa taaaacgaaa ggctcagtcg aaagactggg cctttcgttt tatctgttgt
     4141 ttgtcggtga acgctctcct gagtaggaca aatccgccgg gagcggattt gaacgttgcg
     4201 aagcaacggc ccggagggtg gcgggcagga cgcccgccat aaactgccag gcatcaaatt
     4261 aagcagaagg ccatcctgac ggatggcctt tttgcgtttc tacaaactct tttgtttatt
     4321 tttctaaata cattcaaata tgtatccgct catgagacaa taaccctgat aaatgcttca
     4381 ataatattga aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc ttattccctt
     4441 ttttgcggca ttttgccttc ctgtttttgc tcacccagaa acgctggtga aagtaaaaga
     4501 tgctgaagat cagttgggtg cacgagtggg ttacatcgaa ctggatctca acagcggtaa
     4561 gatccttgag agttttcgcc ccgaagaacg tttcccaatg atgagcactt ttaaagttct
     4621 gctatgtggc gcggtattat cccgtgttga cgccgggcaa gagcaactcg gtcgccgcat
     4681 acactattct cagaatgact tggttgagta ctcaccagtc acagaaaagc atcttacgga
     4741 tggcatgaca gtaagagaat tatgcagtgc tgccataacc atgagtgata acactgcggc
     4801 caacttactt ctgacaacga tcggaggacc gaaggagcta accgcttttt tgcacaacat
     4861 gggggatcat gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa
     4921 cgacgagcgt gacaccacga tgcctgtagc aatggcaaca acgttgcgca aactattaac
     4981 tggcgaacta cttactctag cttcccggca acaattaata gactggatgg aggcggataa
     5041 agttgcagga ccacttctgc gctcggccct tccggctggc tggtttattg ctgataaatc
     5101 tggagccggt gagcgtgggt ctcgcggtat cattgcagca ctggggccag atggtaagcc
     5161 ctcccgtatc gtagttatct acacgacggg gagtcaggca actatggatg aacgaaatag
     5221 acagatcgct gagataggtg cctcactgat taagcattgg taactgtcag accaagttta
     5281 ctcatatata ctttagattg atttaccccg gttgataatc agaaaagccc caaaaacagg
     5341 aagattgtat aagcaaatat ttaaattgta aacgttaata ttttgttaaa attcgcgtta
     5401 aatttttgtt aaatcagctc attttttaac caataggccg aaatcggcaa aatcccttat
     5461 aaatcaaaag aatagcccga gatagggttg agtgttgttc cagtttggaa caagagtcca
     5521 ctattaaaga acgtggactc caacgtcaaa gggcgaaaaa ccgtctatca gggcgatggc
     5581 ccactacgtg aaccatcacc caaatcaagt tttttggggt cgaggtgccg taaagcacta
     5641 aatcggaacc ctaaagggag cccccgattt agagcttgac ggggaaagcc ggcgaacgtg
     5701 gcgagaaagg aagggaagaa agcgaaagga gcgggcgcta gggcgctggc aagtgtagcg
     5761 gtcacgctgc gcgtaaccac cacacccgcc gcgcttaatg cgccgctaca gggcgcgtaa
     5821 aaggatctag gtgaagatcc tttttgataa tctcatgacc aaaatccctt aacgtgagtt
     5881 ttcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt gagatccttt
     5941 ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg
     6001 tttgccggat caagagctac caactctttt tccgaaggta actggcttca gcagagcgca
     6061 gataccaaat actgtccttc tagtgtagcc gtagttaggc caccacttca agaactctgt
     6121 agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg ccagtggcga
     6181 taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg cgcagcggtc
     6241 gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct acaccgaact
     6301 gagataccta cagcgtgagc tatgagaaag cgccacgctt cccgaaggga gaaaggcgga
     6361 caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc ttccaggggg
     6421 aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt
     6481 tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg cggccttttt
     6541 acggttcctg gccttttgct ggccttttgc tcacatgttc tttcctgcgt tatcccctga
     6601 ttctgtggat aaccgtatta ccgcctttga gtgagctgat accgctcgcc gcagccgaac
     6661 gaccgagcgc agcgagtcag tgagcgagga agcggaagag cgcctgatgc ggtattttct
     6721 ccttacgcat ctgtgcggta tttcacaccg catgtggtgc actctcagta caccgcatgt
     6781 ggtgcactct cagtacaatc tgctctgatg ccgcatagtt aagccagtat acactccgct
     6841 atcgctacgt gactgggtca tggctgcgcc ccgacacccg ccaacacccg ctgacgcgcc
     6901 ctgacgggct tgtctgctcc cggcatccgc ttacagacaa gctgtgaccg tctccgggag
     6961 ctgcatgtgt cagaggtttt caccgtcatc accgaaacgc gcgaggcagc tgcggtaaag
     7021 ctcatcagcg tggtcgtgca gcgattcaca gatgtctgcc tgttcatccg cgtccagctc
     7081 gttgagtttc tccagaagcg ttaatgtctg gcttctgata aagcgggcca tgttaagggc
     7141 ggttttttcc tgtttggtca ctgatgcctc cgtgtaaggg ggatttctgt tcatgggggt
     7201 aatgataccg atgaaacgag agaggatgct cacgatacgg gttactgatg atgaacatgc
     7261 ccggttactg gaacgttgtg agggtaaaca actggcggta tggatgcggc gggaccagag
     7321 aaaaatcact cagggtcaat gccagcgctt cgttaataca gatgtaggtg ttccacaggg
     7381 tagccagcag catcctgcga tgcagatccg gaacataatg gtgcagggcg ctgacttccg
     7441 cgtttccaga ctttacgaaa cacggaaacc gaagaccatt catgttgttg ctcaggtcgc
     7501 agacgttttg cagcagcagt cgcttcacgt tcgctcgcgt atcggtgatt cattctgcta
     7561 accagtaagg caaccccgcc agcctagccg ggtcctcaac gacaggagca cgatcatgcg
     7621 cacccgtggc caggacccaa cgctgcccga aattccgaca ccatcgaatg gtgcaaaacc
     7681 tttcgcggta tggcatgata gcgcccggaa gagagtcaat tcagggtggt gaatgtgaaa
     7741 ccagtaacgt tatacgatgt cgcagagtat gccggtgtct cttatcagac cgtttcccgc
     7801 gtggtgaacc aggccagcca cgtttctgcg aaaacgcggg aaaaagtgga agcggcgatg
     7861 gcggagctga attacattcc caaccgcgtg gcacaacaac tggcgggcaa acagtcgttg
     7921 ctgattggcg ttgccacctc cagtctggcc ctgcacgcgc cgtcgcaaat tgtcgcggcg
     7981 attaaatctc gcgccgatca actgggtgcc agcgtggtgg tgtcgatggt agaacgaagc
     8041 ggcgtcgaag cctgtaaagc ggcggtgcac aatcttctcg cgcaacgcgt cagtgggctg
     8101 atcattaact atccgctgga tgaccaggat gccattgctg tggaagctgc ctgcactaat
     8161 gttccggcgt tatttcttga tgtctctgac cagacaccca tcaacagtat tattttctcc
     8221 catgaagacg gtacgcgact gggcgtggag catctggtcg cattgggtca ccagcaaatc
     8281 gcgctgttag cgggcccatt aagttctgtc tcggcgcgtc tgcgtctggc tggctggcat
     8341 aaatatctca ctcgcaatca aattcagccg atagcggaac gggaaggcga ctggagtgcc
     8401 atgtccggtt ttcaacaaac catgcaaatg ctgaatgagg gcatcgttcc cactgcgatg
     8461 ctggttgcca acgatcagat ggcgctgggc gcaatgcgcg ccattaccga gtccgggctg
     8521 cgcgttggtg cggatatctc ggtagtggga tacgacgata ccgaagacag ctcatgttat
     8581 atcccgccgt taaccaccat caaacaggat tttcgcctgc tggggcaaac cagcgtggac
     8641 cgcttgctgc aactctctca gggccaggcg gtgaagggca atcagctgtt gcccgtctca
     8701 ctggtgaaaa gaaaaaccac cctggcgccc aatacgcaaa ccgcctctcc ccgcgcgttg
     8761 gccgattcat taatgcagct ggcacgacag gtttcccgac tggaaagcgg gcagtgagcg
     8821 caacgcaatt aatgtaagtt agctcactca ttaggcacaa ttctcatgtt tgacagctta
     8881 tcatcgactg cacggtgcac caatgcttct ggcgtcaggc agccatcgga agctgtggta
     8941 tggctgtgca ggtcgtaaat cactgcataa ttcgtgtcgc tc
//
