LOCUS       sequence-395172-        9477 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Bacterial protein expression of Dcp2 301-970.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9477)
  AUTHORS   Currie SL, Xing W, Muhlrad D, Decker CJ, Parker R, Rosen MK
  TITLE     Quantitative reconstitution of yeast RNA processing bodies.
  JOURNAL   Proc Natl Acad Sci U S A. 2023 Apr 4;120(14):e2214064120. doi: 
            10.1073/pnas.2214064120. Epub 2023 Mar 27.
  PUBMED    36972455
REFERENCE   2  (bases 1 to 9477)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A."; date: "2023-04-4"; volume: "120(14):e2214064120. 
            doi"; pages: " 10.1073/pnas.2214064120. Epub 2023 Mar 27"
COMMENT     Sequence Label: Dcp2 301-970-EGFP
FEATURES             Location/Qualifiers
     source          1..9477
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        join(67..72,73..88,89..95)
                     /label=tac promoter
                     /note="strong E. coli promoter; hybrid between the trp and 
                     lac UV5 promoters"
     protein_bind    103..119
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     108..130
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     CDS             189..1289
                     /codon_start=1
                     /gene="malE (mutated)"
                     /product="maltose binding protein from E. coli"
                     /label=MBP
                     /note="This version of the gene does not encode a signal 
                     sequence, so MBP will remain in the cytosol."
                     /translation="MKTEEGKLVIWINGDKGYNGLAEVGKKFEKDTGIKVTVEHPDKLE
                     EKFPQVAATGDGPDIIFWAHDRFGGYAQSGLLAEITPDKAFQDKLYPFTWDAVRYNGKL
                     IAYPIAVEALSLIYNKDLLPNPPKTWEEIPALDKELKAKGKSALMFNLQEPYFTWPLIA
                     ADGGYAFKYENGKYDIKDVGVDNAGAKAGLTFLVDLIKNKHMNADTDYSIAEAAFNKGE
                     TAMTINGPWAWSNIDTSKVNYGVTVLPTFKGQPSKPFVGVLSAGINAASPNKELAKEFL
                     ENYLLTDEGLEAVNKDKPLGAVALKSYEEELAKDPRIAATMENAQKGEIMPNIPQMSAF
                     WYAVRTAVINAASGRQTVDEALKDAQT"
     regulatory      1146..1155
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     1263..1286
                     /label=MBP-F
                     /note="Maltose binding protein, forward primer"
     CDS             1338..1349
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     CDS             1362..1382
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             join(3426..3428,3429..3431,3432..4142)
                     /codon_start=1
                     /product="brightly fluorescent monomeric variant of GFP"
                     /label=mEmerald
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHKVYITADKQKNGIK
                     VNFKTRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(3471..3492)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(3732..3751)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     4079..4100
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     CDS             4155..4175
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             4176..4193
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(4206..4223)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(4206..4222)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4215..4237)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     complement(4401..4418)
                     /label=pBAD Reverse
                     /note="For vectors with E. coli araBAD promoter, reverse 
                     primer"
     terminator      4571..4657
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      4749..4776
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     promoter        4795..4886
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(4887..4955,4956..5747)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5105..5124)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      complement(5789..6302)
                     /direction=LEFT
                     /label=M13 ori
                     /note="M13 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5984..6005)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     6196..6215
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     rep_origin      6413..7001
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6902..6921
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     7155..7172
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(7278..7297)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     7438..7460
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     CDS             complement(7454..7645)
                     /codon_start=1
                     /gene="rop"
                     /product="Rop protein, which maintains plasmids at low copy
                     number"
                     /label=rop
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
     promoter        8141..8218
                     /gene="lacI (mutant)"
                     /label=lacIq promoter
                     /note="In the lacIq allele, a single base change in the 
                     promoter boosts expression of the lacI gene about 10-fold."
     CDS             8219..9301
                     /codon_start=1
                     /gene="lacI"
                     /product="lac repressor"
                     /label=lacI
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
                     /translation="MKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAEL
                     NYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERSGV
                     EACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNVPALFLDVSDQTPINSIIFSH
                     EDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREGDWSA
                     MSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDTEDSSC
                     YIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLAPNTQTASPR
                     ALADSLMQLARQVSRLESGQ"
     primer_bind     complement(8238..8257)
                     /label=LacI-R
                     /note="LacI, reverse primer"
ORIGIN
        1 ccgttctgga taatgttttt tgcgccgaca tcataacggt tctggcaaat attctgaaat
       61 gagctgttga caattaatca tcggctcgta taatgtgtgg aattgtgagc ggataacaat
      121 ttcacacagg aaacagccag tccgtttagg tgttttcacg agcacttcac caacaaggac
      181 catagattat gaaaactgaa gaaggtaaac tggtaatctg gattaacggc gataaaggct
      241 ataacggtct cgctgaagtc ggtaagaaat tcgagaaaga taccggaatt aaagtcaccg
      301 ttgagcatcc ggataaactg gaagagaaat tcccacaggt tgcggcaact ggcgatggcc
      361 ctgacattat cttctgggca cacgaccgct ttggtggcta cgctcaatct ggcctgttgg
      421 ctgaaatcac cccggacaaa gcgttccagg acaagctgta tccgtttacc tgggatgccg
      481 tacgttacaa cggcaagctg attgcttacc cgatcgctgt tgaagcgtta tcgctgattt
      541 ataacaaaga tctgctgccg aacccgccaa aaacctggga agagatcccg gcgctggata
      601 aagaactgaa agcgaaaggt aagagcgcgc tgatgttcaa cctgcaagaa ccgtacttca
      661 cctggccgct gattgctgct gacgggggtt atgcgttcaa gtatgaaaac ggcaagtacg
      721 acattaaaga cgtgggcgtg gataacgctg gcgcgaaagc gggtctgacc ttcctggttg
      781 acctgattaa aaacaaacac atgaatgcag acaccgatta ctccatcgca gaagctgcct
      841 ttaataaagg cgaaacagcg atgaccatca acggcccgtg ggcatggtcc aacatcgaca
      901 ccagcaaagt gaattatggt gtaacggtac tgccgacctt caagggtcaa ccatccaaac
      961 cgttcgttgg cgtgctgagc gcaggtatta acgccgccag tccgaacaaa gagctggcaa
     1021 aagagttcct cgaaaactat ctgctgactg atgaaggtct ggaagcggtt aataaagaca
     1081 aaccgctggg tgccgtagcg ctgaagtctt acgaggaaga gttggcgaaa gatccacgta
     1141 ttgccgccac catggaaaac gcccagaaag gtgaaatcat gccgaacatc ccgcagatgt
     1201 ccgctttctg gtatgccgtg cgtactgcgg tgatcaacgc cgccagcggt cgtcagactg
     1261 tcgatgaagc cctgaaagac gcgcagacta attcgagctc gaacaacaac aacaataaca
     1321 ataacaacaa cctcgggatc gagggaagga tttcagaatt tgaaaacctg tattttcagg
     1381 gccatatgct tcagcatttg aaagagcaat caggagaaca caaccaacag aaggatcagc
     1441 agtcatcgtt ttcttctcaa caacaacctt caatatttcc atctctttct gaaccgtttg
     1501 ctaacaataa gaatgttata ccacctacta tgccaatggc taacgtattc atgtcaaatc
     1561 ctcaattgtt tgcgacaatg aatggccagc cttttgcacc tttcccattt atgttaccat
     1621 taactaacaa tagtaatagc gctaacccta ttccaactcc ggtcccccct aattttaatg
     1681 ctcctccgaa tccgatggct tttggtgttc caaacatgca taacctttct ggaccagcag
     1741 tatctcaacc gttttccttg cctcctgctc ctttaccgag ggactctggt tacagcagct
     1801 cctcccctgg gcagttgtta gatatactaa attcgaaaaa gcctgacagc aacgtgcaat
     1861 caagcaaaaa gccaaagctt aaaatcttac agagaggaac ggacttgaat tcaatcaagc
     1921 aaaacaataa tgatgaaact gctcattcaa actctcaagc tttgctagat ttgttgaaaa
     1981 aaccaacatc atcgcagaag atacacgctt ccaaaccaga tacttccttt ttaccaaatg
     2041 actccgtatc tggtatacaa gatgcagaat atgaagattt cgagagtagt tcagatgaag
     2101 aggtggagac agctagagat gaaagaaatt cattgaatgt agatattggg gtgaacgtta
     2161 tgccaagcga aaaagacagc cgaagaagtc aaaaggaaaa accaaggaac gacgcaagca
     2221 aaacaaactt gaacgcttct gcagaatcta atagtgtaga atgggggcct ggtaaatctt
     2281 caccctcgac tcagagtaaa cagaattcat cagtaggaat gcagaataaa tatcgtcaag
     2341 aaattcacat tggtgatagt gatgcgtatg aagtcttcga aagcagttct gacgaagaag
     2401 atggaaaaaa attggaagaa ctggaacaaa ctcaagacaa ctccaaatta attagccaag
     2461 atattttaaa agagaacaat ttccaagacg gtgaagtgcc tcatagagat atgcccactg
     2521 aaagtaataa atctataaac gaaactgtcg ggttatcatc aacaacaaat actgtaaaaa
     2581 aggtacccaa agttaaaata ctaaaacgag gtgaaacctt tgccagtctg gcgaatgata
     2641 aaaaagcttt tgattcaagc tccaatgtgt cttcatcaaa agatttatta cagatgctaa
     2701 gaaacccaat ttcctctact gtatcttcaa atcagcaatc accaaaatct caacatctaa
     2761 gtggggatga agaaatcatg atgatgttga agaggaattc tgtgtcaaaa cctcaaaata
     2821 gcgaagaaaa tgcatcaact tcaagtataa acgatgcgaa tgcaagcgaa cttttgggaa
     2881 tgttaaaaca aaaggaaaaa gacattacag caccaaaaca gccctataat gttgactcat
     2941 actcgcagaa aaattcagct aaaggattgt tgaatatttt gaaaaagaac gactctaccg
     3001 ggtaccctcg cactgagggt ggaccgtcaa gtgaaatgtc aacgtcaatg aaacgcaacg
     3061 acgctacaaa taatcaagaa ttagataaga attcaacaga gttattaaac tacttaaagc
     3121 ccaagcctct taatgatggg tatgagaata tttcaaataa ggacagttct catgaattac
     3181 tgaatatatt gcatggtaat aaaaacagca gcgccttcaa taataatgta tatgcaactg
     3241 acggatattc cttggcatct gataacaacg aaaattcctc aaataaacta ctaaatatgc
     3301 tgcaaaacag gtctagtgca ataaatgagc ccaacttcga cgtccgttcg aatggaactt
     3361 cagggtctaa tgaattatta agcattttgc ataggaaggg atccgggtct ggttcaggaa
     3421 tggccatggt gagcaagggc gaggagctgt tcaccggggt ggtgcccatc ctggtcgagc
     3481 tggacggcga cgtaaacggc cacaagttca gcgtgtccgg cgagggcgag ggcgatgcca
     3541 cctacggcaa gctgaccctg aagttcatct gcaccaccgg caagctgccc gtgccctggc
     3601 ccaccctcgt gaccaccttg acctacggcg tgcagtgctt cgcccgctac cccgaccaca
     3661 tgaagcagca cgacttcttc aagtccgcca tgcccgaagg ctacgtccag gagcgcacca
     3721 tcttcttcaa ggacgacggc aactacaaga cccgcgccga ggtgaagttc gagggcgaca
     3781 ccctggtgaa ccgcatcgag ctgaagggca tcgacttcaa ggaggacggc aacatcctgg
     3841 ggcacaagct ggagtacaac tacaacagcc acaaggtcta tatcaccgcc gacaagcaga
     3901 agaacggcat caaggtgaac ttcaagaccc gccacaacat cgaggacggc agcgtgcagc
     3961 tcgccgacca ctaccagcag aacaccccca tcggcgacgg ccccgtgctg ctgcccgaca
     4021 accactacct gagcacccag tccaagctga gcaaagaccc caacgagaag cgcgatcaca
     4081 tggtcctgct ggagttcgtg accgccgccg ggatcactct cggcatggac gagctgtaca
     4141 agggaagcgg ccgcgaaaac ctgtattttc agggccatca tcatcatcat cactgaaagc
     4201 ttggcactgg ccgtcgtttt acaacgtcgt gactgggaaa accctggcgt tacccaactt
     4261 aatcgccttg cagcacatcc ccctttcgcc agctggcgta atagcgaaga ggcccgcacc
     4321 gatcgccctt cccaacagtt gcgcagcctg aatggcgaat ggcagcttgg ctgttttggc
     4381 ggatgagata agattttcag cctgatacag attaaatcag aacgcagaag cggtctgata
     4441 aaacagaatt tgcctggcgg cagtagcgcg gtggtcccac ctgaccccat gccgaactca
     4501 gaagtgaaac gccgtagcgc cgatggtagt gtggggtctc cccatgcgag agtagggaac
     4561 tgccaggcat caaataaaac gaaaggctca gtcgaaagac tgggcctttc gttttatctg
     4621 ttgtttgtcg gtgaacgctc tcctgagtag gacaaatccg ccgggagcgg atttgaacgt
     4681 tgcgaagcaa cggcccggag ggtggcgggc aggacgcccg ccataaactg ccaggcatca
     4741 aattaagcag aaggccatcc tgacggatgg cctttttgcg tttctacaaa ctcttttgtt
     4801 tatttttcta aatacattca aatatgtatc cgctcatgag acaataaccc tgataaatgc
     4861 ttcaataata ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc gcccttattc
     4921 ccttttttgc ggcattttgc cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa
     4981 aagatgctga agatcagttg ggtgcacgag tgggttacat cgaactggat ctcaacagcg
     5041 gtaagatcct tgagagtttt cgccccgaag aacgtttccc aatgatgagc acttttaaag
     5101 ttctgctatg tggcgcggta ttatcccgtg ttgacgccgg gcaagagcaa ctcggtcgcc
     5161 gcatacacta ttctcagaat gacttggttg agtactcacc agtcacagaa aagcatctta
     5221 cggatggcat gacagtaaga gaattatgca gtgctgccat aaccatgagt gataacactg
     5281 cggccaactt acttctgaca acgatcggag gaccgaagga gctaaccgct tttttgcaca
     5341 acatggggga tcatgtaact cgccttgatc gttgggaacc ggagctgaat gaagccatac
     5401 caaacgacga gcgtgacacc acgatgcctg tagcaatggc aacaacgttg cgcaaactat
     5461 taactggcga actacttact ctagcttccc ggcaacaatt aatagactgg atggaggcgg
     5521 ataaagttgc aggaccactt ctgcgctcgg cccttccggc tggctggttt attgctgata
     5581 aatctggagc cggtgagcgt gggtctcgcg gtatcattgc agcactgggg ccagatggta
     5641 agccctcccg tatcgtagtt atctacacga cggggagtca ggcaactatg gatgaacgaa
     5701 atagacagat cgctgagata ggtgcctcac tgattaagca ttggtaactg tcagaccaag
     5761 tttactcata tatactttag attgatttac cccggttgat aatcagaaaa gccccaaaaa
     5821 caggaagatt gtataagcaa atatttaaat tgtaaacgtt aatattttgt taaaattcgc
     5881 gttaaatttt tgttaaatca gctcattttt taaccaatag gccgaaatcg gcaaaatccc
     5941 ttataaatca aaagaatagc ccgagatagg gttgagtgtt gttccagttt ggaacaagag
     6001 tccactatta aagaacgtgg actccaacgt caaagggcga aaaaccgtct atcagggcga
     6061 tggcccacta cgtgaaccat cacccaaatc aagttttttg gggtcgaggt gccgtaaagc
     6121 actaaatcgg aaccctaaag ggagcccccg atttagagct tgacggggaa agccggcgaa
     6181 cgtggcgaga aaggaaggga agaaagcgaa aggagcgggc gctagggcgc tggcaagtgt
     6241 agcggtcacg ctgcgcgtaa ccaccacacc cgccgcgctt aatgcgccgc tacagggcgc
     6301 gtaaaaggat ctaggtgaag atcctttttg ataatctcat gaccaaaatc ccttaacgtg
     6361 agttttcgtt ccactgagcg tcagaccccg tagaaaagat caaaggatct tcttgagatc
     6421 ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg
     6481 tttgtttgcc ggatcaagag ctaccaactc tttttccgaa ggtaactggc ttcagcagag
     6541 cgcagatacc aaatactgtc cttctagtgt agccgtagtt aggccaccac ttcaagaact
     6601 ctgtagcacc gcctacatac ctcgctctgc taatcctgtt accagtggct gctgccagtg
     6661 gcgataagtc gtgtcttacc gggttggact caagacgata gttaccggat aaggcgcagc
     6721 ggtcgggctg aacggggggt tcgtgcacac agcccagctt ggagcgaacg acctacaccg
     6781 aactgagata cctacagcgt gagctatgag aaagcgccac gcttcccgaa gggagaaagg
     6841 cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg gagcttccag
     6901 ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga cttgagcgtc
     6961 gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccagc aacgcggcct
     7021 ttttacggtt cctggccttt tgctggcctt ttgctcacat gttctttcct gcgttatccc
     7081 ctgattctgt ggataaccgt attaccgcct ttgagtgagc tgataccgct cgccgcagcc
     7141 gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga agagcgcctg atgcggtatt
     7201 ttctccttac gcatctgtgc ggtatttcac accgcatgtg gtgcactctc agtacaccgc
     7261 atgtggtgca ctctcagtac aatctgctct gatgccgcat agttaagcca gtatacactc
     7321 cgctatcgct acgtgactgg gtcatggctg cgccccgaca cccgccaaca cccgctgacg
     7381 cgccctgacg ggcttgtctg ctcccggcat ccgcttacag acaagctgtg accgtctccg
     7441 ggagctgcat gtgtcagagg ttttcaccgt catcaccgaa acgcgcgagg cagctgcggt
     7501 aaagctcatc agcgtggtcg tgcagcgatt cacagatgtc tgcctgttca tccgcgtcca
     7561 gctcgttgag tttctccaga agcgttaatg tctggcttct gataaagcgg gccatgttaa
     7621 gggcggtttt ttcctgtttg gtcactgatg cctccgtgta agggggattt ctgttcatgg
     7681 gggtaatgat accgatgaaa cgagagagga tgctcacgat acgggttact gatgatgaac
     7741 atgcccggtt actggaacgt tgtgagggta aacaactggc ggtatggatg cggcgggacc
     7801 agagaaaaat cactcagggt caatgccagc gcttcgttaa tacagatgta ggtgttccac
     7861 agggtagcca gcagcatcct gcgatgcaga tccggaacat aatggtgcag ggcgctgact
     7921 tccgcgtttc cagactttac gaaacacgga aaccgaagac cattcatgtt gttgctcagg
     7981 tcgcagacgt tttgcagcag cagtcgcttc acgttcgctc gcgtatcggt gattcattct
     8041 gctaaccagt aaggcaaccc cgccagccta gccgggtcct caacgacagg agcacgatca
     8101 tgcgcacccg tggccaggac ccaacgctgc ccgaaattcc gacaccatcg aatggtgcaa
     8161 aacctttcgc ggtatggcat gatagcgccc ggaagagagt caattcaggg tggtgaatgt
     8221 gaaaccagta acgttatacg atgtcgcaga gtatgccggt gtctcttatc agaccgtttc
     8281 ccgcgtggtg aaccaggcca gccacgtttc tgcgaaaacg cgggaaaaag tggaagcggc
     8341 gatggcggag ctgaattaca ttcccaaccg cgtggcacaa caactggcgg gcaaacagtc
     8401 gttgctgatt ggcgttgcca cctccagtct ggccctgcac gcgccgtcgc aaattgtcgc
     8461 ggcgattaaa tctcgcgccg atcaactggg tgccagcgtg gtggtgtcga tggtagaacg
     8521 aagcggcgtc gaagcctgta aagcggcggt gcacaatctt ctcgcgcaac gcgtcagtgg
     8581 gctgatcatt aactatccgc tggatgacca ggatgccatt gctgtggaag ctgcctgcac
     8641 taatgttccg gcgttatttc ttgatgtctc tgaccagaca cccatcaaca gtattatttt
     8701 ctcccatgaa gacggtacgc gactgggcgt ggagcatctg gtcgcattgg gtcaccagca
     8761 aatcgcgctg ttagcgggcc cattaagttc tgtctcggcg cgtctgcgtc tggctggctg
     8821 gcataaatat ctcactcgca atcaaattca gccgatagcg gaacgggaag gcgactggag
     8881 tgccatgtcc ggttttcaac aaaccatgca aatgctgaat gagggcatcg ttcccactgc
     8941 gatgctggtt gccaacgatc agatggcgct gggcgcaatg cgcgccatta ccgagtccgg
     9001 gctgcgcgtt ggtgcggata tctcggtagt gggatacgac gataccgaag acagctcatg
     9061 ttatatcccg ccgttaacca ccatcaaaca ggattttcgc ctgctggggc aaaccagcgt
     9121 ggaccgcttg ctgcaactct ctcagggcca ggcggtgaag ggcaatcagc tgttgcccgt
     9181 ctcactggtg aaaagaaaaa ccaccctggc gcccaatacg caaaccgcct ctccccgcgc
     9241 gttggccgat tcattaatgc agctggcacg acaggtttcc cgactggaaa gcgggcagtg
     9301 agcgcaacgc aattaatgta agttagctca ctcattaggc acaattctca tgtttgacag
     9361 cttatcatcg actgcacggt gcaccaatgc ttctggcgtc aggcagccat cggaagctgt
     9421 ggtatggctg tgcaggtcgt aaatcactgc ataattcgtg tcgctcaagg cgcactc
//
