LOCUS       sequence-395173-       10374 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Bacterial protein expression of Dcp2.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10374)
  AUTHORS   Currie SL, Xing W, Muhlrad D, Decker CJ, Parker R, Rosen MK
  TITLE     Quantitative reconstitution of yeast RNA processing bodies.
  JOURNAL   Proc Natl Acad Sci U S A. 2023 Apr 4;120(14):e2214064120. doi: 
            10.1073/pnas.2214064120. Epub 2023 Mar 27.
  PUBMED    36972455
REFERENCE   2  (bases 1 to 10374)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A."; date: "2023-04-4"; volume: "120(14):e2214064120. 
            doi"; pages: " 10.1073/pnas.2214064120. Epub 2023 Mar 27"
COMMENT     Sequence Label: Dcp2-EGFP
FEATURES             Location/Qualifiers
     source          1..10374
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        join(45..50,51..66,67..73)
                     /label=tac promoter
                     /note="strong E. coli promoter; hybrid between the trp and 
                     lac UV5 promoters"
     protein_bind    81..97
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     86..108
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     CDS             167..1267
                     /codon_start=1
                     /gene="malE (mutated)"
                     /product="maltose binding protein from E. coli"
                     /label=MBP
                     /note="This version of the gene does not encode a signal 
                     sequence, so MBP will remain in the cytosol."
                     /translation="MKTEEGKLVIWINGDKGYNGLAEVGKKFEKDTGIKVTVEHPDKLE
                     EKFPQVAATGDGPDIIFWAHDRFGGYAQSGLLAEITPDKAFQDKLYPFTWDAVRYNGKL
                     IAYPIAVEALSLIYNKDLLPNPPKTWEEIPALDKELKAKGKSALMFNLQEPYFTWPLIA
                     ADGGYAFKYENGKYDIKDVGVDNAGAKAGLTFLVDLIKNKHMNADTDYSIAEAAFNKGE
                     TAMTINGPWAWSNIDTSKVNYGVTVLPTFKGQPSKPFVGVLSAGINAASPNKELAKEFL
                     ENYLLTDEGLEAVNKDKPLGAVALKSYEEELAKDPRIAATMENAQKGEIMPNIPQMSAF
                     WYAVRTAVINAASGRQTVDEALKDAQT"
     regulatory      1124..1133
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     1241..1264
                     /label=MBP-F
                     /note="Maltose binding protein, forward primer"
     CDS             1316..1327
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     CDS             1340..1360
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             join(4301..4303,4304..4306,4307..5017)
                     /codon_start=1
                     /product="brightly fluorescent monomeric variant of GFP"
                     /label=mEmerald
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHKVYITADKQKNGIK
                     VNFKTRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(4346..4367)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(4607..4626)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     4954..4975
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     CDS             5030..5050
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             5051..5068
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(5081..5098)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(5081..5097)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5090..5112)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     complement(5276..5293)
                     /label=pBAD Reverse
                     /note="For vectors with E. coli araBAD promoter, reverse 
                     primer"
     terminator      5446..5532
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      5624..5651
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     promoter        5670..5761
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5762..5830,5831..6622)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5980..5999)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      complement(6664..7177)
                     /direction=LEFT
                     /label=M13 ori
                     /note="M13 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6859..6880)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     7071..7090
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     rep_origin      7288..7876
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7777..7796
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     8030..8047
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(8153..8172)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     8313..8335
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     CDS             complement(8329..8520)
                     /codon_start=1
                     /gene="rop"
                     /product="Rop protein, which maintains plasmids at low copy
                     number"
                     /label=rop
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
     promoter        9016..9093
                     /gene="lacI (mutant)"
                     /label=lacIq promoter
                     /note="In the lacIq allele, a single base change in the 
                     promoter boosts expression of the lacI gene about 10-fold."
     CDS             9094..10176
                     /codon_start=1
                     /gene="lacI"
                     /product="lac repressor"
                     /label=lacI
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
                     /translation="MKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAEL
                     NYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERSGV
                     EACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNVPALFLDVSDQTPINSIIFSH
                     EDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREGDWSA
                     MSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDTEDSSC
                     YIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLAPNTQTASPR
                     ALADSLMQLARQVSRLESGQ"
     primer_bind     complement(9113..9132)
                     /label=LacI-R
                     /note="LacI, reverse primer"
ORIGIN
        1 cgccgacatc ataacggttc tggcaaatat tctgaaatga gctgttgaca attaatcatc
       61 ggctcgtata atgtgtggaa ttgtgagcgg ataacaattt cacacaggaa acagccagtc
      121 cgtttaggtg ttttcacgag cacttcacca acaaggacca tagattatga aaactgaaga
      181 aggtaaactg gtaatctgga ttaacggcga taaaggctat aacggtctcg ctgaagtcgg
      241 taagaaattc gagaaagata ccggaattaa agtcaccgtt gagcatccgg ataaactgga
      301 agagaaattc ccacaggttg cggcaactgg cgatggccct gacattatct tctgggcaca
      361 cgaccgcttt ggtggctacg ctcaatctgg cctgttggct gaaatcaccc cggacaaagc
      421 gttccaggac aagctgtatc cgtttacctg ggatgccgta cgttacaacg gcaagctgat
      481 tgcttacccg atcgctgttg aagcgttatc gctgatttat aacaaagatc tgctgccgaa
      541 cccgccaaaa acctgggaag agatcccggc gctggataaa gaactgaaag cgaaaggtaa
      601 gagcgcgctg atgttcaacc tgcaagaacc gtacttcacc tggccgctga ttgctgctga
      661 cgggggttat gcgttcaagt atgaaaacgg caagtacgac attaaagacg tgggcgtgga
      721 taacgctggc gcgaaagcgg gtctgacctt cctggttgac ctgattaaaa acaaacacat
      781 gaatgcagac accgattact ccatcgcaga agctgccttt aataaaggcg aaacagcgat
      841 gaccatcaac ggcccgtggg catggtccaa catcgacacc agcaaagtga attatggtgt
      901 aacggtactg ccgaccttca agggtcaacc atccaaaccg ttcgttggcg tgctgagcgc
      961 aggtattaac gccgccagtc cgaacaaaga gctggcaaaa gagttcctcg aaaactatct
     1021 gctgactgat gaaggtctgg aagcggttaa taaagacaaa ccgctgggtg ccgtagcgct
     1081 gaagtcttac gaggaagagt tggcgaaaga tccacgtatt gccgccacca tggaaaacgc
     1141 ccagaaaggt gaaatcatgc cgaacatccc gcagatgtcc gctttctggt atgccgtgcg
     1201 tactgcggtg atcaacgccg ccagcggtcg tcagactgtc gatgaagccc tgaaagacgc
     1261 gcagactaat tcgagctcga acaacaacaa caataacaat aacaacaacc tcgggatcga
     1321 gggaaggatt tcagaatttg aaaacctgta ttttcagggc catatgtcac tgccgctacg
     1381 acacgcattg gagaacgtta cttctgttga tagaatttta gaggacttat tagtacgttt
     1441 tattataaat tgtccgaatg aagatttatc gagtgtcgag agagagttat ttcattttga
     1501 agaagcctca tggttttaca cggatttcat caaattgatg aatccaactt taccctccct
     1561 aaagattaaa tcatttgctc aattgatcat aaaactatgt cctctggttt ggaaatggga
     1621 cataagagtg gatgaggcac tccagcaatt ctccaagtat aagaaaagta taccggtgag
     1681 gggcgctgcc atatttaacg agaacctgag taaaatttta ttggtacagg gtactgaatc
     1741 ggattcttgg tcattcccaa gggggaagat atctaaagat gaaaatgaca tagattgttg
     1801 cattagagaa gtgaaagaag aaattggttt cgatttgacg gactatattg acgacaacca
     1861 attcattgaa agaaatattc aaggtaaaaa ttacaaaata tttttgatat ctggtgtttc
     1921 agaagtcttc aattttaaac ctcaagttag aaatgaaatt gataagatag aatggttcga
     1981 ttttaagaaa atttctaaaa caatgtacaa atcaaatatc aagtattatc tgattaattc
     2041 catgatgaga cccttatcaa tgtggttaag gcatcagagg caaataaaaa atgaagatca
     2101 attgaaatcc tatgcggaag aacaattgaa attgttgttg ggtatcacta aggaggagca
     2161 gattgatccc ggtagagagt tgctgaatat gttacatact gcagtgcaag ctaacagtaa
     2221 taataatgcg gtctccaacg gacaggtacc ctcgagccaa gagcttcagc atttgaaaga
     2281 gcaatcagga gaacacaacc aacagaagga tcagcagtca tcgttttctt ctcaacaaca
     2341 accttcaata tttccatctc tttctgaacc gtttgctaac aataagaatg ttataccacc
     2401 tactatgcca atggctaacg tattcatgtc aaatcctcaa ttgtttgcga caatgaatgg
     2461 ccagcctttt gcacctttcc catttatgtt accattaact aacaatagta atagcgctaa
     2521 ccctattcca actccggtcc cccctaattt taatgctcct ccgaatccga tggcttttgg
     2581 tgttccaaac atgcataacc tttctggacc agcagtatct caaccgtttt ccttgcctcc
     2641 tgctccttta ccgagggact ctggttacag cagctcctcc cctgggcagt tgttagatat
     2701 actaaattcg aaaaagcctg acagcaacgt gcaatcaagc aaaaagccaa agcttaaaat
     2761 cttacagaga ggaacggact tgaattcaat caagcaaaac aataatgatg aaactgctca
     2821 ttcaaactct caagctttgc tagatttgtt gaaaaaacca acatcatcgc agaagataca
     2881 cgcttccaaa ccagatactt cctttttacc aaatgactcc gtatctggta tacaagatgc
     2941 agaatatgaa gatttcgaga gtagttcaga tgaagaggtg gagacagcta gagatgaaag
     3001 aaattcattg aatgtagata ttggggtgaa cgttatgcca agcgaaaaag acagccgaag
     3061 aagtcaaaag gaaaaaccaa ggaacgacgc aagcaaaaca aacttgaacg cttctgcaga
     3121 atctaatagt gtagaatggg ggcctggtaa atcttcaccc tcgactcaga gtaaacagaa
     3181 ttcatcagta ggaatgcaga ataaatatcg tcaagaaatt cacattggtg atagtgatgc
     3241 gtatgaagtc ttcgaaagca gttctgacga agaagatgga aaaaaattgg aagaactgga
     3301 acaaactcaa gacaactcca aattaattag ccaagatatt ttaaaagaga acaatttcca
     3361 agacggtgaa gtgcctcata gagatatgcc cactgaaagt aataaatcta taaacgaaac
     3421 tgtcgggtta tcatcaacaa caaatactgt aaaaaaggta cccaaagtta aaatactaaa
     3481 acgaggtgaa acctttgcca gtctggcgaa tgataaaaaa gcttttgatt caagctccaa
     3541 tgtgtcttca tcaaaagatt tattacagat gctaagaaac ccaatttcct ctactgtatc
     3601 ttcaaatcag caatcaccaa aatctcaaca tctaagtggg gatgaagaaa tcatgatgat
     3661 gttgaagagg aattctgtgt caaaacctca aaatagcgaa gaaaatgcat caacttcaag
     3721 tataaacgat gcgaatgcaa gcgaactttt gggaatgtta aaacaaaagg aaaaagacat
     3781 tacagcacca aaacagccct ataatgttga ctcatactcg cagaaaaatt cagctaaagg
     3841 attgttgaat attttgaaaa agaacgactc taccgggtac cctcgcactg agggtggacc
     3901 gtcaagtgaa atgtcaacgt caatgaaacg caacgacgct acaaataatc aagaattaga
     3961 taagaattca acagagttat taaactactt aaagcccaag cctcttaatg atgggtatga
     4021 gaatatttca aataaggaca gttctcatga attactgaat atattgcatg gtaataaaaa
     4081 cagcagcgcc ttcaataata atgtatatgc aactgacgga tattccttgg catctgataa
     4141 caacgaaaat tcctcaaata aactactaaa tatgctgcaa aacaggtcta gtgcaataaa
     4201 tgagcccaac ttcgacgtcc gttcgaatgg aacttcaggg tctaatgaat tattaagcat
     4261 tttgcatagg aagggatccg ggtctggttc aggaatggcc atggtgagca agggcgagga
     4321 gctgttcacc ggggtggtgc ccatcctggt cgagctggac ggcgacgtaa acggccacaa
     4381 gttcagcgtg tccggcgagg gcgagggcga tgccacctac ggcaagctga ccctgaagtt
     4441 catctgcacc accggcaagc tgcccgtgcc ctggcccacc ctcgtgacca ccttgaccta
     4501 cggcgtgcag tgcttcgccc gctaccccga ccacatgaag cagcacgact tcttcaagtc
     4561 cgccatgccc gaaggctacg tccaggagcg caccatcttc ttcaaggacg acggcaacta
     4621 caagacccgc gccgaggtga agttcgaggg cgacaccctg gtgaaccgca tcgagctgaa
     4681 gggcatcgac ttcaaggagg acggcaacat cctggggcac aagctggagt acaactacaa
     4741 cagccacaag gtctatatca ccgccgacaa gcagaagaac ggcatcaagg tgaacttcaa
     4801 gacccgccac aacatcgagg acggcagcgt gcagctcgcc gaccactacc agcagaacac
     4861 ccccatcggc gacggccccg tgctgctgcc cgacaaccac tacctgagca cccagtccaa
     4921 gctgagcaaa gaccccaacg agaagcgcga tcacatggtc ctgctggagt tcgtgaccgc
     4981 cgccgggatc actctcggca tggacgagct gtacaaggga agcggccgcg aaaacctgta
     5041 ttttcagggc catcatcatc atcatcactg aaagcttggc actggccgtc gttttacaac
     5101 gtcgtgactg ggaaaaccct ggcgttaccc aacttaatcg ccttgcagca catccccctt
     5161 tcgccagctg gcgtaatagc gaagaggccc gcaccgatcg cccttcccaa cagttgcgca
     5221 gcctgaatgg cgaatggcag cttggctgtt ttggcggatg agataagatt ttcagcctga
     5281 tacagattaa atcagaacgc agaagcggtc tgataaaaca gaatttgcct ggcggcagta
     5341 gcgcggtggt cccacctgac cccatgccga actcagaagt gaaacgccgt agcgccgatg
     5401 gtagtgtggg gtctccccat gcgagagtag ggaactgcca ggcatcaaat aaaacgaaag
     5461 gctcagtcga aagactgggc ctttcgtttt atctgttgtt tgtcggtgaa cgctctcctg
     5521 agtaggacaa atccgccggg agcggatttg aacgttgcga agcaacggcc cggagggtgg
     5581 cgggcaggac gcccgccata aactgccagg catcaaatta agcagaaggc catcctgacg
     5641 gatggccttt ttgcgtttct acaaactctt ttgtttattt ttctaaatac attcaaatat
     5701 gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa aaaggaagag
     5761 tatgagtatt caacatttcc gtgtcgccct tattcccttt tttgcggcat tttgccttcc
     5821 tgtttttgct cacccagaaa cgctggtgaa agtaaaagat gctgaagatc agttgggtgc
     5881 acgagtgggt tacatcgaac tggatctcaa cagcggtaag atccttgaga gttttcgccc
     5941 cgaagaacgt ttcccaatga tgagcacttt taaagttctg ctatgtggcg cggtattatc
     6001 ccgtgttgac gccgggcaag agcaactcgg tcgccgcata cactattctc agaatgactt
     6061 ggttgagtac tcaccagtca cagaaaagca tcttacggat ggcatgacag taagagaatt
     6121 atgcagtgct gccataacca tgagtgataa cactgcggcc aacttacttc tgacaacgat
     6181 cggaggaccg aaggagctaa ccgctttttt gcacaacatg ggggatcatg taactcgcct
     6241 tgatcgttgg gaaccggagc tgaatgaagc cataccaaac gacgagcgtg acaccacgat
     6301 gcctgtagca atggcaacaa cgttgcgcaa actattaact ggcgaactac ttactctagc
     6361 ttcccggcaa caattaatag actggatgga ggcggataaa gttgcaggac cacttctgcg
     6421 ctcggccctt ccggctggct ggtttattgc tgataaatct ggagccggtg agcgtgggtc
     6481 tcgcggtatc attgcagcac tggggccaga tggtaagccc tcccgtatcg tagttatcta
     6541 cacgacgggg agtcaggcaa ctatggatga acgaaataga cagatcgctg agataggtgc
     6601 ctcactgatt aagcattggt aactgtcaga ccaagtttac tcatatatac tttagattga
     6661 tttaccccgg ttgataatca gaaaagcccc aaaaacagga agattgtata agcaaatatt
     6721 taaattgtaa acgttaatat tttgttaaaa ttcgcgttaa atttttgtta aatcagctca
     6781 ttttttaacc aataggccga aatcggcaaa atcccttata aatcaaaaga atagcccgag
     6841 atagggttga gtgttgttcc agtttggaac aagagtccac tattaaagaa cgtggactcc
     6901 aacgtcaaag ggcgaaaaac cgtctatcag ggcgatggcc cactacgtga accatcaccc
     6961 aaatcaagtt ttttggggtc gaggtgccgt aaagcactaa atcggaaccc taaagggagc
     7021 ccccgattta gagcttgacg gggaaagccg gcgaacgtgg cgagaaagga agggaagaaa
     7081 gcgaaaggag cgggcgctag ggcgctggca agtgtagcgg tcacgctgcg cgtaaccacc
     7141 acacccgccg cgcttaatgc gccgctacag ggcgcgtaaa aggatctagg tgaagatcct
     7201 ttttgataat ctcatgacca aaatccctta acgtgagttt tcgttccact gagcgtcaga
     7261 ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg
     7321 cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc
     7381 aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgtccttct
     7441 agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc
     7501 tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt
     7561 ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg
     7621 cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct
     7681 atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag
     7741 ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag
     7801 tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg
     7861 gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg
     7921 gccttttgct cacatgttct ttcctgcgtt atcccctgat tctgtggata accgtattac
     7981 cgcctttgag tgagctgata ccgctcgccg cagccgaacg accgagcgca gcgagtcagt
     8041 gagcgaggaa gcggaagagc gcctgatgcg gtattttctc cttacgcatc tgtgcggtat
     8101 ttcacaccgc atgtggtgca ctctcagtac accgcatgtg gtgcactctc agtacaatct
     8161 gctctgatgc cgcatagtta agccagtata cactccgcta tcgctacgtg actgggtcat
     8221 ggctgcgccc cgacacccgc caacacccgc tgacgcgccc tgacgggctt gtctgctccc
     8281 ggcatccgct tacagacaag ctgtgaccgt ctccgggagc tgcatgtgtc agaggttttc
     8341 accgtcatca ccgaaacgcg cgaggcagct gcggtaaagc tcatcagcgt ggtcgtgcag
     8401 cgattcacag atgtctgcct gttcatccgc gtccagctcg ttgagtttct ccagaagcgt
     8461 taatgtctgg cttctgataa agcgggccat gttaagggcg gttttttcct gtttggtcac
     8521 tgatgcctcc gtgtaagggg gatttctgtt catgggggta atgataccga tgaaacgaga
     8581 gaggatgctc acgatacggg ttactgatga tgaacatgcc cggttactgg aacgttgtga
     8641 gggtaaacaa ctggcggtat ggatgcggcg ggaccagaga aaaatcactc agggtcaatg
     8701 ccagcgcttc gttaatacag atgtaggtgt tccacagggt agccagcagc atcctgcgat
     8761 gcagatccgg aacataatgg tgcagggcgc tgacttccgc gtttccagac tttacgaaac
     8821 acggaaaccg aagaccattc atgttgttgc tcaggtcgca gacgttttgc agcagcagtc
     8881 gcttcacgtt cgctcgcgta tcggtgattc attctgctaa ccagtaaggc aaccccgcca
     8941 gcctagccgg gtcctcaacg acaggagcac gatcatgcgc acccgtggcc aggacccaac
     9001 gctgcccgaa attccgacac catcgaatgg tgcaaaacct ttcgcggtat ggcatgatag
     9061 cgcccggaag agagtcaatt cagggtggtg aatgtgaaac cagtaacgtt atacgatgtc
     9121 gcagagtatg ccggtgtctc ttatcagacc gtttcccgcg tggtgaacca ggccagccac
     9181 gtttctgcga aaacgcggga aaaagtggaa gcggcgatgg cggagctgaa ttacattccc
     9241 aaccgcgtgg cacaacaact ggcgggcaaa cagtcgttgc tgattggcgt tgccacctcc
     9301 agtctggccc tgcacgcgcc gtcgcaaatt gtcgcggcga ttaaatctcg cgccgatcaa
     9361 ctgggtgcca gcgtggtggt gtcgatggta gaacgaagcg gcgtcgaagc ctgtaaagcg
     9421 gcggtgcaca atcttctcgc gcaacgcgtc agtgggctga tcattaacta tccgctggat
     9481 gaccaggatg ccattgctgt ggaagctgcc tgcactaatg ttccggcgtt atttcttgat
     9541 gtctctgacc agacacccat caacagtatt attttctccc atgaagacgg tacgcgactg
     9601 ggcgtggagc atctggtcgc attgggtcac cagcaaatcg cgctgttagc gggcccatta
     9661 agttctgtct cggcgcgtct gcgtctggct ggctggcata aatatctcac tcgcaatcaa
     9721 attcagccga tagcggaacg ggaaggcgac tggagtgcca tgtccggttt tcaacaaacc
     9781 atgcaaatgc tgaatgaggg catcgttccc actgcgatgc tggttgccaa cgatcagatg
     9841 gcgctgggcg caatgcgcgc cattaccgag tccgggctgc gcgttggtgc ggatatctcg
     9901 gtagtgggat acgacgatac cgaagacagc tcatgttata tcccgccgtt aaccaccatc
     9961 aaacaggatt ttcgcctgct ggggcaaacc agcgtggacc gcttgctgca actctctcag
    10021 ggccaggcgg tgaagggcaa tcagctgttg cccgtctcac tggtgaaaag aaaaaccacc
    10081 ctggcgccca atacgcaaac cgcctctccc cgcgcgttgg ccgattcatt aatgcagctg
    10141 gcacgacagg tttcccgact ggaaagcggg cagtgagcgc aacgcaatta atgtaagtta
    10201 gctcactcat taggcacaat tctcatgttt gacagcttat catcgactgc acggtgcacc
    10261 aatgcttctg gcgtcaggca gccatcggaa gctgtggtat ggctgtgcag gtcgtaaatc
    10321 actgcataat tcgtgtcgct caaggcgcac tcccgttctg gataatgttt tttg
//
