LOCUS       sequence-395176-       10374 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Bacterial protein expression of Dcp2 H237A.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10374)
  AUTHORS   Currie SL, Xing W, Muhlrad D, Decker CJ, Parker R, Rosen MK
  TITLE     Quantitative reconstitution of yeast RNA processing bodies.
  JOURNAL   Proc Natl Acad Sci U S A. 2023 Apr 4;120(14):e2214064120. doi: 
            10.1073/pnas.2214064120. Epub 2023 Mar 27.
  PUBMED    36972455
REFERENCE   2  (bases 1 to 10374)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A."; date: "2023-04-4"; volume: "120(14):e2214064120. 
            doi"; pages: " 10.1073/pnas.2214064120. Epub 2023 Mar 27"
COMMENT     Sequence Label: Dcp2 H237A-EGFP
FEATURES             Location/Qualifiers
     source          1..10374
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        join(16..21,22..37,38..44)
                     /label=tac promoter
                     /note="strong E. coli promoter; hybrid between the trp and 
                     lac UV5 promoters"
     protein_bind    52..68
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     57..79
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     CDS             138..1238
                     /codon_start=1
                     /gene="malE (mutated)"
                     /product="maltose binding protein from E. coli"
                     /label=MBP
                     /note="This version of the gene does not encode a signal 
                     sequence, so MBP will remain in the cytosol."
                     /translation="MKTEEGKLVIWINGDKGYNGLAEVGKKFEKDTGIKVTVEHPDKLE
                     EKFPQVAATGDGPDIIFWAHDRFGGYAQSGLLAEITPDKAFQDKLYPFTWDAVRYNGKL
                     IAYPIAVEALSLIYNKDLLPNPPKTWEEIPALDKELKAKGKSALMFNLQEPYFTWPLIA
                     ADGGYAFKYENGKYDIKDVGVDNAGAKAGLTFLVDLIKNKHMNADTDYSIAEAAFNKGE
                     TAMTINGPWAWSNIDTSKVNYGVTVLPTFKGQPSKPFVGVLSAGINAASPNKELAKEFL
                     ENYLLTDEGLEAVNKDKPLGAVALKSYEEELAKDPRIAATMENAQKGEIMPNIPQMSAF
                     WYAVRTAVINAASGRQTVDEALKDAQT"
     regulatory      1095..1104
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     1212..1235
                     /label=MBP-F
                     /note="Maltose binding protein, forward primer"
     CDS             1287..1298
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     CDS             1311..1331
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             join(4272..4274,4275..4277,4278..4988)
                     /codon_start=1
                     /product="brightly fluorescent monomeric variant of GFP"
                     /label=mEmerald
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHKVYITADKQKNGIK
                     VNFKTRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(4317..4338)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(4578..4597)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     4925..4946
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     CDS             5001..5021
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             5022..5039
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(5052..5069)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(5052..5068)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5061..5083)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     complement(5247..5264)
                     /label=pBAD Reverse
                     /note="For vectors with E. coli araBAD promoter, reverse 
                     primer"
     terminator      5417..5503
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      5595..5622
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     promoter        5641..5732
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5733..5801,5802..6593)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5951..5970)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      complement(6635..7148)
                     /direction=LEFT
                     /label=M13 ori
                     /note="M13 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6830..6851)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     7042..7061
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     rep_origin      7259..7847
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7748..7767
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     8001..8018
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(8124..8143)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     8284..8306
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     CDS             complement(8300..8491)
                     /codon_start=1
                     /gene="rop"
                     /product="Rop protein, which maintains plasmids at low copy
                     number"
                     /label=rop
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
     promoter        8987..9064
                     /gene="lacI (mutant)"
                     /label=lacIq promoter
                     /note="In the lacIq allele, a single base change in the 
                     promoter boosts expression of the lacI gene about 10-fold."
     CDS             9065..10147
                     /codon_start=1
                     /gene="lacI"
                     /product="lac repressor"
                     /label=lacI
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
                     /translation="MKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAEL
                     NYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERSGV
                     EACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNVPALFLDVSDQTPINSIIFSH
                     EDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREGDWSA
                     MSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDTEDSSC
                     YIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLAPNTQTASPR
                     ALADSLMQLARQVSRLESGQ"
     primer_bind     complement(9084..9103)
                     /label=LacI-R
                     /note="LacI, reverse primer"
ORIGIN
        1 ttctgaaatg agctgttgac aattaatcat cggctcgtat aatgtgtgga attgtgagcg
       61 gataacaatt tcacacagga aacagccagt ccgtttaggt gttttcacga gcacttcacc
      121 aacaaggacc atagattatg aaaactgaag aaggtaaact ggtaatctgg attaacggcg
      181 ataaaggcta taacggtctc gctgaagtcg gtaagaaatt cgagaaagat accggaatta
      241 aagtcaccgt tgagcatccg gataaactgg aagagaaatt cccacaggtt gcggcaactg
      301 gcgatggccc tgacattatc ttctgggcac acgaccgctt tggtggctac gctcaatctg
      361 gcctgttggc tgaaatcacc ccggacaaag cgttccagga caagctgtat ccgtttacct
      421 gggatgccgt acgttacaac ggcaagctga ttgcttaccc gatcgctgtt gaagcgttat
      481 cgctgattta taacaaagat ctgctgccga acccgccaaa aacctgggaa gagatcccgg
      541 cgctggataa agaactgaaa gcgaaaggta agagcgcgct gatgttcaac ctgcaagaac
      601 cgtacttcac ctggccgctg attgctgctg acgggggtta tgcgttcaag tatgaaaacg
      661 gcaagtacga cattaaagac gtgggcgtgg ataacgctgg cgcgaaagcg ggtctgacct
      721 tcctggttga cctgattaaa aacaaacaca tgaatgcaga caccgattac tccatcgcag
      781 aagctgcctt taataaaggc gaaacagcga tgaccatcaa cggcccgtgg gcatggtcca
      841 acatcgacac cagcaaagtg aattatggtg taacggtact gccgaccttc aagggtcaac
      901 catccaaacc gttcgttggc gtgctgagcg caggtattaa cgccgccagt ccgaacaaag
      961 agctggcaaa agagttcctc gaaaactatc tgctgactga tgaaggtctg gaagcggtta
     1021 ataaagacaa accgctgggt gccgtagcgc tgaagtctta cgaggaagag ttggcgaaag
     1081 atccacgtat tgccgccacc atggaaaacg cccagaaagg tgaaatcatg ccgaacatcc
     1141 cgcagatgtc cgctttctgg tatgccgtgc gtactgcggt gatcaacgcc gccagcggtc
     1201 gtcagactgt cgatgaagcc ctgaaagacg cgcagactaa ttcgagctcg aacaacaaca
     1261 acaataacaa taacaacaac ctcgggatcg agggaaggat ttcagaattt gaaaacctgt
     1321 attttcaggg ccatatgtca ctgccgctac gacacgcatt ggagaacgtt acttctgttg
     1381 atagaatttt agaggactta ttagtacgtt ttattataaa ttgtccgaat gaagatttat
     1441 cgagtgtcga gagagagtta tttcattttg aagaagcctc atggttttac acggatttca
     1501 tcaaattgat gaatccaact ttaccctccc taaagattaa atcatttgct caattgatca
     1561 taaaactatg tcctctggtt tggaaatggg acataagagt ggatgaggca ctccagcaat
     1621 tctccaagta taagaaaagt ataccggtga ggggcgctgc catatttaac gagaacctga
     1681 gtaaaatttt attggtacag ggtactgaat cggattcttg gtcattccca agggggaaga
     1741 tatctaaaga tgaaaatgac atagattgtt gcattagaga agtgaaagaa gaaattggtt
     1801 tcgatttgac ggactatatt gacgacaacc aattcattga aagaaatatt caaggtaaaa
     1861 attacaaaat atttttgata tctggtgttt cagaagtctt caattttaaa cctcaagtta
     1921 gaaatgaaat tgataagata gaatggttcg attttaagaa aatttctaaa acaatgtaca
     1981 aatcaaatat caagtattat ctgattaatt ccatgatgag acccttatca atgtggttaa
     2041 gggctcagag gcaaataaaa aatgaagatc aattgaaatc ctatgcggaa gaacaattga
     2101 aattgttgtt gggtatcact aaggaggagc agattgatcc cggtagagag ttgctgaata
     2161 tgttacatac tgcagtgcaa gctaacagta ataataatgt ggtctccaac ggacaggtac
     2221 cctcgagcca agagcttcag catttgaaag agcaatcagg agaacacaac caacagaagg
     2281 atcagcagtc atcgttttct tctcaacaac aaccttcaat atttccatct ctttctgaac
     2341 cgtttgctaa caataagaat gttataccac ctactatgcc aatggctaac gtattcatgt
     2401 caaatcctca attgtttgcg acaatgaatg gccagccttt tgcacctttc ccatttatgt
     2461 taccattaac taacaatagt aatagcgcta accctattcc aactccggtc ccccctaatt
     2521 ttaatgctcc tccgaatccg atggcttttg gtgttccaaa catgcataac ctttctggac
     2581 cagcagtatc tcaaccgttt tccttgcctc ctgctccttt accgagggac tctggttaca
     2641 gcagctcctc ccctgggcag ttgttagata tactaaattc gaaaaagcct gacagcaacg
     2701 tgcaatcaag caaaaagcca aagcttaaaa tcttacagag aggaacggac ttgaattcaa
     2761 tcaagcaaaa caataatgat gaaactgctc attcaaactc tcaagctttg ctagatttgt
     2821 tgaaaaaacc aacatcatcg cagaagatac acgcttccaa accagatact tcctttttac
     2881 caaatgactc cgtatctggt atacaagatg cagaatatga agatttcgag agtagttcag
     2941 atgaagaggt ggagacagct agagatgaaa gaaattcatt gaatgtagat attggggtga
     3001 acgttatgcc aagcgaaaaa gacagccgaa gaagtcaaaa ggaaaaacca aggaacgacg
     3061 caagcaaaac aaacttgaac gcttctgcag aatctaatag tgtagaatgg gggcctggta
     3121 aatcttcacc ctcgactcag agtaaacaga attcatcagt aggaatgcag aataaatatc
     3181 gtcaagaaat tcacattggt gatagtgatg cgtatgaagt cttcgaaagc agttctgacg
     3241 aagaagatgg aaaaaaattg gaagaactgg aacaaactca agacaactcc aaattaatta
     3301 gccaagatat tttaaaagag aacaatttcc aagacggtga agtgcctcat agagatatgc
     3361 ccactgaaag taataaatct ataaacgaaa ctgtcgggtt atcatcaaca acaaatactg
     3421 taaaaaaggt acccaaagtt aaaatactaa aacgaggtga aacctttgcc agtctggcga
     3481 atgataaaaa agcttttgat tcaagctcca atgtgtcttc atcaaaagat ttattacaga
     3541 tgctaagaaa cccaatttcc tctactgtat cttcaaatca gcaatcacca aaatctcaac
     3601 atctaagtgg ggatgaagaa atcatgatga tgttgaagag gaattctgtg tcaaaacctc
     3661 aaaatagcga agaaaatgca tcaacttcaa gtataaacga tgcgaatgca agcgaacttt
     3721 tgggaatgtt aaaacaaaag gaaaaagaca ttacagcacc aaaacagccc tataatgttg
     3781 actcatactc gcagaaaaat tcagctaaag gattgttgaa tattttgaaa aagaacgact
     3841 ctaccgggta ccctcgcact gagggtggac cgtcaagtga aatgtcaacg tcaatgaaac
     3901 gcaacgacgc tacaaataat caagaattag ataagaattc aacagagtta ttaaactact
     3961 taaagcccaa gcctcttaat gatgggtatg agaatatttc aaataaggac agttctcatg
     4021 aattactgaa tatattgcat ggtaataaaa acagcagcgc cttcaataat aatgtatatg
     4081 caactgacgg atattccttg gcatctgata acaacgaaaa ttcctcaaat aaactactaa
     4141 atatgctgca aaacaggtct agtgcaataa atgagcccaa cttcgacgtc cgttcgaatg
     4201 gaacttcagg gtctaatgaa ttattaagca ttttgcatag gaagggatcc gggtctggtt
     4261 caggaatggc catggtgagc aagggcgagg agctgttcac cggggtggtg cccatcctgg
     4321 tcgagctgga cggcgacgta aacggccaca agttcagcgt gtccggcgag ggcgagggcg
     4381 atgccaccta cggcaagctg accctgaagt tcatctgcac caccggcaag ctgcccgtgc
     4441 cctggcccac cctcgtgacc accttgacct acggcgtgca gtgcttcgcc cgctaccccg
     4501 accacatgaa gcagcacgac ttcttcaagt ccgccatgcc cgaaggctac gtccaggagc
     4561 gcaccatctt cttcaaggac gacggcaact acaagacccg cgccgaggtg aagttcgagg
     4621 gcgacaccct ggtgaaccgc atcgagctga agggcatcga cttcaaggag gacggcaaca
     4681 tcctggggca caagctggag tacaactaca acagccacaa ggtctatatc accgccgaca
     4741 agcagaagaa cggcatcaag gtgaacttca agacccgcca caacatcgag gacggcagcg
     4801 tgcagctcgc cgaccactac cagcagaaca cccccatcgg cgacggcccc gtgctgctgc
     4861 ccgacaacca ctacctgagc acccagtcca agctgagcaa agaccccaac gagaagcgcg
     4921 atcacatggt cctgctggag ttcgtgaccg ccgccgggat cactctcggc atggacgagc
     4981 tgtacaaggg aagcggccgc gaaaacctgt attttcaggg ccatcatcat catcatcact
     5041 gaaagcttgg cactggccgt cgttttacaa cgtcgtgact gggaaaaccc tggcgttacc
     5101 caacttaatc gccttgcagc acatccccct ttcgccagct ggcgtaatag cgaagaggcc
     5161 cgcaccgatc gcccttccca acagttgcgc agcctgaatg gcgaatggca gcttggctgt
     5221 tttggcggat gagataagat tttcagcctg atacagatta aatcagaacg cagaagcggt
     5281 ctgataaaac agaatttgcc tggcggcagt agcgcggtgg tcccacctga ccccatgccg
     5341 aactcagaag tgaaacgccg tagcgccgat ggtagtgtgg ggtctcccca tgcgagagta
     5401 gggaactgcc aggcatcaaa taaaacgaaa ggctcagtcg aaagactggg cctttcgttt
     5461 tatctgttgt ttgtcggtga acgctctcct gagtaggaca aatccgccgg gagcggattt
     5521 gaacgttgcg aagcaacggc ccggagggtg gcgggcagga cgcccgccat aaactgccag
     5581 gcatcaaatt aagcagaagg ccatcctgac ggatggcctt tttgcgtttc tacaaactct
     5641 tttgtttatt tttctaaata cattcaaata tgtatccgct catgagacaa taaccctgat
     5701 aaatgcttca ataatattga aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc
     5761 ttattccctt ttttgcggca ttttgccttc ctgtttttgc tcacccagaa acgctggtga
     5821 aagtaaaaga tgctgaagat cagttgggtg cacgagtggg ttacatcgaa ctggatctca
     5881 acagcggtaa gatccttgag agttttcgcc ccgaagaacg tttcccaatg atgagcactt
     5941 ttaaagttct gctatgtggc gcggtattat cccgtgttga cgccgggcaa gagcaactcg
     6001 gtcgccgcat acactattct cagaatgact tggttgagta ctcaccagtc acagaaaagc
     6061 atcttacgga tggcatgaca gtaagagaat tatgcagtgc tgccataacc atgagtgata
     6121 acactgcggc caacttactt ctgacaacga tcggaggacc gaaggagcta accgcttttt
     6181 tgcacaacat gggggatcat gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag
     6241 ccataccaaa cgacgagcgt gacaccacga tgcctgtagc aatggcaaca acgttgcgca
     6301 aactattaac tggcgaacta cttactctag cttcccggca acaattaata gactggatgg
     6361 aggcggataa agttgcagga ccacttctgc gctcggccct tccggctggc tggtttattg
     6421 ctgataaatc tggagccggt gagcgtgggt ctcgcggtat cattgcagca ctggggccag
     6481 atggtaagcc ctcccgtatc gtagttatct acacgacggg gagtcaggca actatggatg
     6541 aacgaaatag acagatcgct gagataggtg cctcactgat taagcattgg taactgtcag
     6601 accaagttta ctcatatata ctttagattg atttaccccg gttgataatc agaaaagccc
     6661 caaaaacagg aagattgtat aagcaaatat ttaaattgta aacgttaata ttttgttaaa
     6721 attcgcgtta aatttttgtt aaatcagctc attttttaac caataggccg aaatcggcaa
     6781 aatcccttat aaatcaaaag aatagcccga gatagggttg agtgttgttc cagtttggaa
     6841 caagagtcca ctattaaaga acgtggactc caacgtcaaa gggcgaaaaa ccgtctatca
     6901 gggcgatggc ccactacgtg aaccatcacc caaatcaagt tttttggggt cgaggtgccg
     6961 taaagcacta aatcggaacc ctaaagggag cccccgattt agagcttgac ggggaaagcc
     7021 ggcgaacgtg gcgagaaagg aagggaagaa agcgaaagga gcgggcgcta gggcgctggc
     7081 aagtgtagcg gtcacgctgc gcgtaaccac cacacccgcc gcgcttaatg cgccgctaca
     7141 gggcgcgtaa aaggatctag gtgaagatcc tttttgataa tctcatgacc aaaatccctt
     7201 aacgtgagtt ttcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt
     7261 gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag
     7321 cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta actggcttca
     7381 gcagagcgca gataccaaat actgtccttc tagtgtagcc gtagttaggc caccacttca
     7441 agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg
     7501 ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg
     7561 cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct
     7621 acaccgaact gagataccta cagcgtgagc tatgagaaag cgccacgctt cccgaaggga
     7681 gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc
     7741 ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg
     7801 agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg
     7861 cggccttttt acggttcctg gccttttgct ggccttttgc tcacatgttc tttcctgcgt
     7921 tatcccctga ttctgtggat aaccgtatta ccgcctttga gtgagctgat accgctcgcc
     7981 gcagccgaac gaccgagcgc agcgagtcag tgagcgagga agcggaagag cgcctgatgc
     8041 ggtattttct ccttacgcat ctgtgcggta tttcacaccg catgtggtgc actctcagta
     8101 caccgcatgt ggtgcactct cagtacaatc tgctctgatg ccgcatagtt aagccagtat
     8161 acactccgct atcgctacgt gactgggtca tggctgcgcc ccgacacccg ccaacacccg
     8221 ctgacgcgcc ctgacgggct tgtctgctcc cggcatccgc ttacagacaa gctgtgaccg
     8281 tctccgggag ctgcatgtgt cagaggtttt caccgtcatc accgaaacgc gcgaggcagc
     8341 tgcggtaaag ctcatcagcg tggtcgtgca gcgattcaca gatgtctgcc tgttcatccg
     8401 cgtccagctc gttgagtttc tccagaagcg ttaatgtctg gcttctgata aagcgggcca
     8461 tgttaagggc ggttttttcc tgtttggtca ctgatgcctc cgtgtaaggg ggatttctgt
     8521 tcatgggggt aatgataccg atgaaacgag agaggatgct cacgatacgg gttactgatg
     8581 atgaacatgc ccggttactg gaacgttgtg agggtaaaca actggcggta tggatgcggc
     8641 gggaccagag aaaaatcact cagggtcaat gccagcgctt cgttaataca gatgtaggtg
     8701 ttccacaggg tagccagcag catcctgcga tgcagatccg gaacataatg gtgcagggcg
     8761 ctgacttccg cgtttccaga ctttacgaaa cacggaaacc gaagaccatt catgttgttg
     8821 ctcaggtcgc agacgttttg cagcagcagt cgcttcacgt tcgctcgcgt atcggtgatt
     8881 cattctgcta accagtaagg caaccccgcc agcctagccg ggtcctcaac gacaggagca
     8941 cgatcatgcg cacccgtggc caggacccaa cgctgcccga aattccgaca ccatcgaatg
     9001 gtgcaaaacc tttcgcggta tggcatgata gcgcccggaa gagagtcaat tcagggtggt
     9061 gaatgtgaaa ccagtaacgt tatacgatgt cgcagagtat gccggtgtct cttatcagac
     9121 cgtttcccgc gtggtgaacc aggccagcca cgtttctgcg aaaacgcggg aaaaagtgga
     9181 agcggcgatg gcggagctga attacattcc caaccgcgtg gcacaacaac tggcgggcaa
     9241 acagtcgttg ctgattggcg ttgccacctc cagtctggcc ctgcacgcgc cgtcgcaaat
     9301 tgtcgcggcg attaaatctc gcgccgatca actgggtgcc agcgtggtgg tgtcgatggt
     9361 agaacgaagc ggcgtcgaag cctgtaaagc ggcggtgcac aatcttctcg cgcaacgcgt
     9421 cagtgggctg atcattaact atccgctgga tgaccaggat gccattgctg tggaagctgc
     9481 ctgcactaat gttccggcgt tatttcttga tgtctctgac cagacaccca tcaacagtat
     9541 tattttctcc catgaagacg gtacgcgact gggcgtggag catctggtcg cattgggtca
     9601 ccagcaaatc gcgctgttag cgggcccatt aagttctgtc tcggcgcgtc tgcgtctggc
     9661 tggctggcat aaatatctca ctcgcaatca aattcagccg atagcggaac gggaaggcga
     9721 ctggagtgcc atgtccggtt ttcaacaaac catgcaaatg ctgaatgagg gcatcgttcc
     9781 cactgcgatg ctggttgcca acgatcagat ggcgctgggc gcaatgcgcg ccattaccga
     9841 gtccgggctg cgcgttggtg cggatatctc ggtagtggga tacgacgata ccgaagacag
     9901 ctcatgttat atcccgccgt taaccaccat caaacaggat tttcgcctgc tggggcaaac
     9961 cagcgtggac cgcttgctgc aactctctca gggccaggcg gtgaagggca atcagctgtt
    10021 gcccgtctca ctggtgaaaa gaaaaaccac cctggcgccc aatacgcaaa ccgcctctcc
    10081 ccgcgcgttg gccgattcat taatgcagct ggcacgacag gtttcccgac tggaaagcgg
    10141 gcagtgagcg caacgcaatt aatgtaagtt agctcactca ttaggcacaa ttctcatgtt
    10201 tgacagctta tcatcgactg cacggtgcac caatgcttct ggcgtcaggc agccatcgga
    10261 agctgtggta tggctgtgca ggtcgtaaat cactgcataa ttcgtgtcgc tcaaggcgca
    10321 ctcccgttct ggataatgtt ttttgcgccg acatcataac ggttctggca aata
//
