LOCUS       sequence-395179-        8670 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Bacterial protein expression of Dhh1 25-425.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8670)
  AUTHORS   Currie SL, Xing W, Muhlrad D, Decker CJ, Parker R, Rosen MK
  TITLE     Quantitative reconstitution of yeast RNA processing bodies.
  JOURNAL   Proc Natl Acad Sci U S A. 2023 Apr 4;120(14):e2214064120. doi: 
            10.1073/pnas.2214064120. Epub 2023 Mar 27.
  PUBMED    36972455
REFERENCE   2  (bases 1 to 8670)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A."; date: "2023-04-4"; volume: "120(14):e2214064120. 
            doi"; pages: " 10.1073/pnas.2214064120. Epub 2023 Mar 27"
COMMENT     Sequence Label: Dhh1 25-425-EGFP
FEATURES             Location/Qualifiers
     source          1..8670
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        join(31..36,37..52,53..59)
                     /label=tac promoter
                     /note="strong E. coli promoter; hybrid between the trp and 
                     lac UV5 promoters"
     protein_bind    67..83
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     72..94
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     CDS             153..1253
                     /codon_start=1
                     /gene="malE (mutated)"
                     /product="maltose binding protein from E. coli"
                     /label=MBP
                     /note="This version of the gene does not encode a signal 
                     sequence, so MBP will remain in the cytosol."
                     /translation="MKTEEGKLVIWINGDKGYNGLAEVGKKFEKDTGIKVTVEHPDKLE
                     EKFPQVAATGDGPDIIFWAHDRFGGYAQSGLLAEITPDKAFQDKLYPFTWDAVRYNGKL
                     IAYPIAVEALSLIYNKDLLPNPPKTWEEIPALDKELKAKGKSALMFNLQEPYFTWPLIA
                     ADGGYAFKYENGKYDIKDVGVDNAGAKAGLTFLVDLIKNKHMNADTDYSIAEAAFNKGE
                     TAMTINGPWAWSNIDTSKVNYGVTVLPTFKGQPSKPFVGVLSAGINAASPNKELAKEFL
                     ENYLLTDEGLEAVNKDKPLGAVALKSYEEELAKDPRIAATMENAQKGEIMPNIPQMSAF
                     WYAVRTAVINAASGRQTVDEALKDAQT"
     regulatory      1110..1119
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     1227..1250
                     /label=MBP-F
                     /note="Maltose binding protein, forward primer"
     CDS             1302..1313
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     CDS             1326..1346
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             join(2583..2585,2586..2588,2589..3299)
                     /codon_start=1
                     /product="brightly fluorescent monomeric variant of GFP"
                     /label=mEmerald
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHKVYITADKQKNGIK
                     VNFKTRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(2628..2649)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(2889..2908)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     3236..3257
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     CDS             3312..3332
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             3333..3350
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(3363..3380)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(3363..3379)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3372..3394)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     complement(3558..3575)
                     /label=pBAD Reverse
                     /note="For vectors with E. coli araBAD promoter, reverse 
                     primer"
     terminator      3728..3814
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      3906..3933
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     promoter        3952..4043
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(4044..4112,4113..4904)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(4262..4281)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      complement(4946..5459)
                     /direction=LEFT
                     /label=M13 ori
                     /note="M13 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5141..5162)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     5353..5372
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     rep_origin      5570..6158
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6059..6078
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     6312..6329
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(6435..6454)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     6595..6617
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     CDS             complement(6611..6802)
                     /codon_start=1
                     /gene="rop"
                     /product="Rop protein, which maintains plasmids at low copy
                     number"
                     /label=rop
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
     promoter        7298..7375
                     /gene="lacI (mutant)"
                     /label=lacIq promoter
                     /note="In the lacIq allele, a single base change in the 
                     promoter boosts expression of the lacI gene about 10-fold."
     CDS             7376..8458
                     /codon_start=1
                     /gene="lacI"
                     /product="lac repressor"
                     /label=lacI
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
                     /translation="MKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAEL
                     NYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERSGV
                     EACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNVPALFLDVSDQTPINSIIFSH
                     EDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREGDWSA
                     MSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDTEDSSC
                     YIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLAPNTQTASPR
                     ALADSLMQLARQVSRLESGQ"
     primer_bind     complement(7395..7414)
                     /label=LacI-R
                     /note="LacI, reverse primer"
ORIGIN
        1 cggttctggc aaatattctg aaatgagctg ttgacaatta atcatcggct cgtataatgt
       61 gtggaattgt gagcggataa caatttcaca caggaaacag ccagtccgtt taggtgtttt
      121 cacgagcact tcaccaacaa ggaccataga ttatgaaaac tgaagaaggt aaactggtaa
      181 tctggattaa cggcgataaa ggctataacg gtctcgctga agtcggtaag aaattcgaga
      241 aagataccgg aattaaagtc accgttgagc atccggataa actggaagag aaattcccac
      301 aggttgcggc aactggcgat ggccctgaca ttatcttctg ggcacacgac cgctttggtg
      361 gctacgctca atctggcctg ttggctgaaa tcaccccgga caaagcgttc caggacaagc
      421 tgtatccgtt tacctgggat gccgtacgtt acaacggcaa gctgattgct tacccgatcg
      481 ctgttgaagc gttatcgctg atttataaca aagatctgct gccgaacccg ccaaaaacct
      541 gggaagagat cccggcgctg gataaagaac tgaaagcgaa aggtaagagc gcgctgatgt
      601 tcaacctgca agaaccgtac ttcacctggc cgctgattgc tgctgacggg ggttatgcgt
      661 tcaagtatga aaacggcaag tacgacatta aagacgtggg cgtggataac gctggcgcga
      721 aagcgggtct gaccttcctg gttgacctga ttaaaaacaa acacatgaat gcagacaccg
      781 attactccat cgcagaagct gcctttaata aaggcgaaac agcgatgacc atcaacggcc
      841 cgtgggcatg gtccaacatc gacaccagca aagtgaatta tggtgtaacg gtactgccga
      901 ccttcaaggg tcaaccatcc aaaccgttcg ttggcgtgct gagcgcaggt attaacgccg
      961 ccagtccgaa caaagagctg gcaaaagagt tcctcgaaaa ctatctgctg actgatgaag
     1021 gtctggaagc ggttaataaa gacaaaccgc tgggtgccgt agcgctgaag tcttacgagg
     1081 aagagttggc gaaagatcca cgtattgccg ccaccatgga aaacgcccag aaaggtgaaa
     1141 tcatgccgaa catcccgcag atgtccgctt tctggtatgc cgtgcgtact gcggtgatca
     1201 acgccgccag cggtcgtcag actgtcgatg aagccctgaa agacgcgcag actaattcga
     1261 gctcgaacaa caacaacaat aacaataaca acaacctcgg gatcgaggga aggatttcag
     1321 aatttgaaaa cctgtatttt cagggccata tggcattgaa tattcccaag aaggatacca
     1381 gaccacagac agatgatgtc ttaaatacaa agggtaatac ttttgaagat ttttatttga
     1441 aaagagagct gttaatgggt atttttgaag ccggttttga aaaaccatcc cctattcaag
     1501 aagaggctat tccggtagca ataactggta gagacatatt ggccagagca aagaatggta
     1561 caggtaagac agccgcattt gttattccta cgttagagaa agtcaaacca aagttgaata
     1621 aaattcaggc tttgatcatg gttcccacaa gagagttagc tctgcaaact tcccaagtcg
     1681 tccgtacatt gggaaaacac tgtggtattt catgtatggt gaccactggt ggtaccaatt
     1741 tgagagacga tattctaaga ttaaatgaaa cagtccatat tttggttggt actcctggta
     1801 gagtgttgga tttagcttcg agaaaagtag cagatctgtc tgattgttca ttattcatca
     1861 tggacgaagc cgataaaatg ttatctcgtg acttcaagac aataattgaa caaatcttat
     1921 ccttcttacc tccaactcac caatctttat tatttagcgc tactttccca ctaacggtta
     1981 aggaattcat ggttaaacat ctgcataagc cttatgaaat taatttgatg gaagaattga
     2041 ctctaaaggg tattactcaa tactacgcct ttgttgaaga aaggcaaaag ctacattgtt
     2101 taaatacttt attctctaag cttcaaatta atcaagccat tattttttgt aattctacta
     2161 accgtgtcga attactagcc aagaaaatca ctgatttagg ctattcctgt tattactctc
     2221 atgcaagaat gaagcaacaa gaaagaaata aagttttcca cgaatttcgt caaggtaagg
     2281 ttcgtacatt ggtctgctcc gatttattga cccgtggtat cgatattcaa gccgttaatg
     2341 tcgttatcaa tttcgatttc cccaaaacgg cagaaacata tttacatcgt attggtagat
     2401 ccggtaggtt tggccacttg ggtttagcaa tcaatttgat taactggaat gatcggttta
     2461 acctttataa aatcgaacaa gaactgggca ctgaaattgc ggctattccg gccactatcg
     2521 ataagtcatt gtatgtggct gaaaatgacg aaacgggatc cgggtctggt tcaggaatgg
     2581 ccatggtgag caagggcgag gagctgttca ccggggtggt gcccatcctg gtcgagctgg
     2641 acggcgacgt aaacggccac aagttcagcg tgtccggcga gggcgagggc gatgccacct
     2701 acggcaagct gaccctgaag ttcatctgca ccaccggcaa gctgcccgtg ccctggccca
     2761 ccctcgtgac caccttgacc tacggcgtgc agtgcttcgc ccgctacccc gaccacatga
     2821 agcagcacga cttcttcaag tccgccatgc ccgaaggcta cgtccaggag cgcaccatct
     2881 tcttcaagga cgacggcaac tacaagaccc gcgccgaggt gaagttcgag ggcgacaccc
     2941 tggtgaaccg catcgagctg aagggcatcg acttcaagga ggacggcaac atcctggggc
     3001 acaagctgga gtacaactac aacagccaca aggtctatat caccgccgac aagcagaaga
     3061 acggcatcaa ggtgaacttc aagacccgcc acaacatcga ggacggcagc gtgcagctcg
     3121 ccgaccacta ccagcagaac acccccatcg gcgacggccc cgtgctgctg cccgacaacc
     3181 actacctgag cacccagtcc aagctgagca aagaccccaa cgagaagcgc gatcacatgg
     3241 tcctgctgga gttcgtgacc gccgccggga tcactctcgg catggacgag ctgtacaagg
     3301 gaagcggccg cgaaaacctg tattttcagg gccatcatca tcatcatcac tgaaagcttg
     3361 gcactggccg tcgttttaca acgtcgtgac tgggaaaacc ctggcgttac ccaacttaat
     3421 cgccttgcag cacatccccc tttcgccagc tggcgtaata gcgaagaggc ccgcaccgat
     3481 cgcccttccc aacagttgcg cagcctgaat ggcgaatggc agcttggctg ttttggcgga
     3541 tgagataaga ttttcagcct gatacagatt aaatcagaac gcagaagcgg tctgataaaa
     3601 cagaatttgc ctggcggcag tagcgcggtg gtcccacctg accccatgcc gaactcagaa
     3661 gtgaaacgcc gtagcgccga tggtagtgtg gggtctcccc atgcgagagt agggaactgc
     3721 caggcatcaa ataaaacgaa aggctcagtc gaaagactgg gcctttcgtt ttatctgttg
     3781 tttgtcggtg aacgctctcc tgagtaggac aaatccgccg ggagcggatt tgaacgttgc
     3841 gaagcaacgg cccggagggt ggcgggcagg acgcccgcca taaactgcca ggcatcaaat
     3901 taagcagaag gccatcctga cggatggcct ttttgcgttt ctacaaactc ttttgtttat
     3961 ttttctaaat acattcaaat atgtatccgc tcatgagaca ataaccctga taaatgcttc
     4021 aataatattg aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc cttattccct
     4081 tttttgcggc attttgcctt cctgtttttg ctcacccaga aacgctggtg aaagtaaaag
     4141 atgctgaaga tcagttgggt gcacgagtgg gttacatcga actggatctc aacagcggta
     4201 agatccttga gagttttcgc cccgaagaac gtttcccaat gatgagcact tttaaagttc
     4261 tgctatgtgg cgcggtatta tcccgtgttg acgccgggca agagcaactc ggtcgccgca
     4321 tacactattc tcagaatgac ttggttgagt actcaccagt cacagaaaag catcttacgg
     4381 atggcatgac agtaagagaa ttatgcagtg ctgccataac catgagtgat aacactgcgg
     4441 ccaacttact tctgacaacg atcggaggac cgaaggagct aaccgctttt ttgcacaaca
     4501 tgggggatca tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa gccataccaa
     4561 acgacgagcg tgacaccacg atgcctgtag caatggcaac aacgttgcgc aaactattaa
     4621 ctggcgaact acttactcta gcttcccggc aacaattaat agactggatg gaggcggata
     4681 aagttgcagg accacttctg cgctcggccc ttccggctgg ctggtttatt gctgataaat
     4741 ctggagccgg tgagcgtggg tctcgcggta tcattgcagc actggggcca gatggtaagc
     4801 cctcccgtat cgtagttatc tacacgacgg ggagtcaggc aactatggat gaacgaaata
     4861 gacagatcgc tgagataggt gcctcactga ttaagcattg gtaactgtca gaccaagttt
     4921 actcatatat actttagatt gatttacccc ggttgataat cagaaaagcc ccaaaaacag
     4981 gaagattgta taagcaaata tttaaattgt aaacgttaat attttgttaa aattcgcgtt
     5041 aaatttttgt taaatcagct cattttttaa ccaataggcc gaaatcggca aaatccctta
     5101 taaatcaaaa gaatagcccg agatagggtt gagtgttgtt ccagtttgga acaagagtcc
     5161 actattaaag aacgtggact ccaacgtcaa agggcgaaaa accgtctatc agggcgatgg
     5221 cccactacgt gaaccatcac ccaaatcaag ttttttgggg tcgaggtgcc gtaaagcact
     5281 aaatcggaac cctaaaggga gcccccgatt tagagcttga cggggaaagc cggcgaacgt
     5341 ggcgagaaag gaagggaaga aagcgaaagg agcgggcgct agggcgctgg caagtgtagc
     5401 ggtcacgctg cgcgtaacca ccacacccgc cgcgcttaat gcgccgctac agggcgcgta
     5461 aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt
     5521 tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt
     5581 tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt
     5641 gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc
     5701 agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg
     5761 tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg
     5821 ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt
     5881 cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac
     5941 tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg
     6001 acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg
     6061 gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat
     6121 ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt
     6181 tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg
     6241 attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa
     6301 cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc
     6361 tccttacgca tctgtgcggt atttcacacc gcatgtggtg cactctcagt acaccgcatg
     6421 tggtgcactc tcagtacaat ctgctctgat gccgcatagt taagccagta tacactccgc
     6481 tatcgctacg tgactgggtc atggctgcgc cccgacaccc gccaacaccc gctgacgcgc
     6541 cctgacgggc ttgtctgctc ccggcatccg cttacagaca agctgtgacc gtctccggga
     6601 gctgcatgtg tcagaggttt tcaccgtcat caccgaaacg cgcgaggcag ctgcggtaaa
     6661 gctcatcagc gtggtcgtgc agcgattcac agatgtctgc ctgttcatcc gcgtccagct
     6721 cgttgagttt ctccagaagc gttaatgtct ggcttctgat aaagcgggcc atgttaaggg
     6781 cggttttttc ctgtttggtc actgatgcct ccgtgtaagg gggatttctg ttcatggggg
     6841 taatgatacc gatgaaacga gagaggatgc tcacgatacg ggttactgat gatgaacatg
     6901 cccggttact ggaacgttgt gagggtaaac aactggcggt atggatgcgg cgggaccaga
     6961 gaaaaatcac tcagggtcaa tgccagcgct tcgttaatac agatgtaggt gttccacagg
     7021 gtagccagca gcatcctgcg atgcagatcc ggaacataat ggtgcagggc gctgacttcc
     7081 gcgtttccag actttacgaa acacggaaac cgaagaccat tcatgttgtt gctcaggtcg
     7141 cagacgtttt gcagcagcag tcgcttcacg ttcgctcgcg tatcggtgat tcattctgct
     7201 aaccagtaag gcaaccccgc cagcctagcc gggtcctcaa cgacaggagc acgatcatgc
     7261 gcacccgtgg ccaggaccca acgctgcccg aaattccgac accatcgaat ggtgcaaaac
     7321 ctttcgcggt atggcatgat agcgcccgga agagagtcaa ttcagggtgg tgaatgtgaa
     7381 accagtaacg ttatacgatg tcgcagagta tgccggtgtc tcttatcaga ccgtttcccg
     7441 cgtggtgaac caggccagcc acgtttctgc gaaaacgcgg gaaaaagtgg aagcggcgat
     7501 ggcggagctg aattacattc ccaaccgcgt ggcacaacaa ctggcgggca aacagtcgtt
     7561 gctgattggc gttgccacct ccagtctggc cctgcacgcg ccgtcgcaaa ttgtcgcggc
     7621 gattaaatct cgcgccgatc aactgggtgc cagcgtggtg gtgtcgatgg tagaacgaag
     7681 cggcgtcgaa gcctgtaaag cggcggtgca caatcttctc gcgcaacgcg tcagtgggct
     7741 gatcattaac tatccgctgg atgaccagga tgccattgct gtggaagctg cctgcactaa
     7801 tgttccggcg ttatttcttg atgtctctga ccagacaccc atcaacagta ttattttctc
     7861 ccatgaagac ggtacgcgac tgggcgtgga gcatctggtc gcattgggtc accagcaaat
     7921 cgcgctgtta gcgggcccat taagttctgt ctcggcgcgt ctgcgtctgg ctggctggca
     7981 taaatatctc actcgcaatc aaattcagcc gatagcggaa cgggaaggcg actggagtgc
     8041 catgtccggt tttcaacaaa ccatgcaaat gctgaatgag ggcatcgttc ccactgcgat
     8101 gctggttgcc aacgatcaga tggcgctggg cgcaatgcgc gccattaccg agtccgggct
     8161 gcgcgttggt gcggatatct cggtagtggg atacgacgat accgaagaca gctcatgtta
     8221 tatcccgccg ttaaccacca tcaaacagga ttttcgcctg ctggggcaaa ccagcgtgga
     8281 ccgcttgctg caactctctc agggccaggc ggtgaagggc aatcagctgt tgcccgtctc
     8341 actggtgaaa agaaaaacca ccctggcgcc caatacgcaa accgcctctc cccgcgcgtt
     8401 ggccgattca ttaatgcagc tggcacgaca ggtttcccga ctggaaagcg ggcagtgagc
     8461 gcaacgcaat taatgtaagt tagctcactc attaggcaca attctcatgt ttgacagctt
     8521 atcatcgact gcacggtgca ccaatgcttc tggcgtcagg cagccatcgg aagctgtggt
     8581 atggctgtgc aggtcgtaaa tcactgcata attcgtgtcg ctcaaggcgc actcccgttc
     8641 tggataatgt tttttgcgcc gacatcataa
//
