LOCUS       sequence-39518-e        5492 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5492)
  AUTHORS   Kobayashi T, Antar AA, Boehme KW, Danthi P, Eby EA, Guglielmi KM, 
            Holm GH, Johnson EM, Maginnis MS, Naik S, Skelton WB, Wetzel JD, 
            Wilson GJ, Chappell JD, Dermody TS
  TITLE     A plasmid-based reverse genetics system for animal double-stranded 
            RNA viruses.
  JOURNAL   Cell Host Microbe. 2007 Apr 19;1(2):147-57.
  PUBMED    18005692
REFERENCE   2  (bases 1 to 5492)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell Host 
            Microbe."; date: "2007-04-19"; volume: "1(2)"; pages: "147-57"
COMMENT     Sequence Label: pT7-M2T1L
FEATURES             Location/Qualifiers
     source          1..5492
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(2593..2611)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      2607..2654
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     promoter        complement(2722..2740)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(2723..2740)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(3031..3050)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     primer_bind     complement(3261..3280)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     3380..3402
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(3440..3458)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        3526..3630
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(3631..3699,3700..4491)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(3849..3868)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      4662..5250
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5151..5170
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     5404..5421
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 atcgatcccc taggttgttc tctatctttg tgatgtggct ctgaaacaaa ccaggtgtga
       61 ttacagtaac aatttcaatt taaattccga tataaatttc aatgagagga aaattattaa
      121 tcttcctcat agttattcgc acacaaaaga tcctaaaaat tcttctttct ttttgtgtgt
      181 ccggggtcgg catggcatct ccacctcctc gcggtccgac ctggggttaa tacgactcac
      241 tatagctaat ctgctgaccg tcactctgca aagatgggga acgcttcctc tatcgttcag
      301 acgatcaacg ttactggaga tggcaatgta ttcaaaccct cagctgagac ttcatccacc
      361 gctgtaccgt cactgagtct atcacctgga atgttaaatc ctggaggagt accatggatt
      421 gcgattggag atgaaacatc cgttacttca ccgggtgcat tgcggcgaat gacttcgaag
      481 gatatcccag agacagcaat aatcaatacg gataattcat caggcgcggt gccaagtgaa
      541 tcagcattag tgccctataa tgatgagcca ttggtagtgg tgacggagca cgctatcgca
      601 aacttcacca aagccgagat ggcacttgag ttcaatcgtg agttcctcga taaattgcgc
      661 gtgctgtcag tgtcaccaaa atattccgac cttctgacgt atgttgattg ttacgttggt
      721 gtgtcggccc gtcaggccct aaataatttc cagaagcagg tgcctgtgat tacacctact
      781 agacaaacaa tgtatgttga ctccatacag gcggccttga aagccttaga gaaatgggaa
      841 attgatttga gagtggctca gacactgttg cctacaaatg tcccaattgg ggaggtctct
      901 tgtccaatgc agtcagtagt gaaactatta gacgatcagc tgccggacga tagccttata
      961 cggaggtatc ctaaggaggc cgctgttgct ttggctaaaa ggaatggagg gatacagtgg
     1021 atggacgtat cagaaggaac tgtgatgaat gaggccgtga atgctgtcgc agcaagtgcc
     1081 ctggcacctt ccgcatcagc cccaccctta gaagagaaat cgaaattgac tgagcaagcg
     1141 atggatcttg taactgcagc tgaacctgag ataatcgcat ctctcgtacc agttccagcg
     1201 cccgtgtttg ccattccacc taagccagcc gactataatg tgcgtacttt gaagatcgat
     1261 gaggccacat ggttacgaat gatcccaaaa actatgggta cgccttttca aatccaagtg
     1321 actgacaaca caggaactaa ctggcacctt aacttgagag gagggacacg cgtagtgaac
     1381 ttggatcaga ttgctccgat gagattcgtt ctggatctgg ggggaaaaag ctataaggag
     1441 acaagttggg atccaaatgg taagaaggtt gggtttatcg tatttcaatc taagattcct
     1501 tttgagcttt ggaccgccgc ctcacagatt ggccaagcta cagtggttaa ctatgttcag
     1561 ctatatgctg aagacagctc atttaccgcc cagtccatca tcgccactac atcgttggct
     1621 tataattatg aacctgagca attgaataag actgaccctg agatgaacta ttacctttta
     1681 gcgactttca tagactcagc tgctataaca ccgacaaaca tgacacagcc tgatgtttgg
     1741 gatgctctgt tgacgatgtc tccactgtcc gctggagagg tgactgtgaa gggtgcggta
     1801 gtaagcgagg tggtcccagc ggagttgata gggagctata ctccagagtc actaaatgcc
     1861 tcacttccga atgacgctgc tagatgtatg atcgatagag cttcgaagat agccgaggct
     1921 ataaaaattg atgatgacgc cgggccagac gaatactctc ccaactctgt accgatccaa
     1981 ggtcagcttg ctatttctca acttgagact gggtatggtg tacggatatt caatcccaaa
     2041 ggaattctct caaaaatcgc atctagagct atgcaggctt ttattggtga tccaagtaca
     2101 attattacgc aggcagcacc ggtgctgtca gataagaata attggattgc attggcacaa
     2161 ggagttaaga ctagtttgcg tactaaaagt ttatcggcgg gggtgaagac ggcggtgagt
     2221 aagctgagct catccgagtc tattcaaaat tggactcaag gattcttgga taaggtatcg
     2281 acgcactttc cagcgcctaa gcccgactgt ccgaccaacg gagatggcag tgaaccgtct
     2341 gctcggcgag tgaagcgcga ctcatacgca ggagtggtta agcgtgggta tacacgctaa
     2401 gctgctcgcc ctggtgacgc ggggttaagg gtgcaggcac atcatcgggt cggcatggca
     2461 tctccacctc ctcgcggtcc gacctgggca tccgaaggag gacgtcgtcc actcggatgg
     2521 ctaagggaga gctcaaaaaa aaggatccgg ctgctaacaa agcccgaaag gaagctgagt
     2581 tggctgctgc caccgctgag caataactag cataacccct tggggcctct aaacgggtct
     2641 tgaggggttt tttgctgaaa ggaggaacta tatccggatc gagatcctct agagtcgacc
     2701 tgcaggcatg caagcttgta ttctatagtg tcacctaaat cgtatgtgta tgatacataa
     2761 ggttatgtat taattgtagc cgcgttctaa cgacaatatg tacaagccta attgtgtagc
     2821 atctggctta ctgaagcaga ccctatcatc tctctcgtaa actgccgtca gagtcggttt
     2881 ggttggacga accttctgag tttctggtaa cgccgttccg caccccggaa atggtcagcg
     2941 aaccaatcag cagggtcatc gctagccaga tcctctacgc cggacgcatc gtggccggca
     3001 tcaccggcgc cacaggtgcg gttgctggcg cctatatcgc cgacatcacc gatggggaag
     3061 atcgggctcg ccacttcggg ctcatgagcg cttgtttcgg cgtgggtatg gtggcaggcc
     3121 ccgtggccgg gggactgttg ggcgccatct ccttgcacca ttccttgcgg cggcggtgct
     3181 caacggcctc aacctactac tgggctgctt cctaatgcag gagtcgcata agggagagcg
     3241 tcgatatggt gcactctcag tacaatctgc tctgatgccg catagttaag ccagccccga
     3301 cacccgccaa cacccgctga cgcgccctga cgggcttgtc tgctcccggc atccgcttac
     3361 agacaagctg tgaccgtctc cgggagctgc atgtgtcaga ggttttcacc gtcatcaccg
     3421 aaacgcgcga gacgaaaggg cctcgtgata cgcctatttt tataggttaa tgtcatgata
     3481 ataatggttt cttagacgtc aggtggcact tttcggggaa atgtgcgcgg aacccctatt
     3541 tgtttatttt tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa
     3601 atgcttcaat aatattgaaa aaggaagagt atgagtattc aacatttccg tgtcgccctt
     3661 attccctttt ttgcggcatt ttgccttcct gtttttgctc acccagaaac gctggtgaaa
     3721 gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt acatcgaact ggatctcaac
     3781 agcggtaaga tccttgagag ttttcgcccc gaagaacgtt ttccaatgat gagcactttt
     3841 aaagttctgc tatgtggcgc ggtattatcc cgtattgacg ccgggcaaga gcaactcggt
     3901 cgccgcatac actattctca gaatgacttg gttgagtact caccagtcac agaaaagcat
     3961 cttacggatg gcatgacagt aagagaatta tgcagtgctg ccataaccat gagtgataac
     4021 actgcggcca acttacttct gacaacgatc ggaggaccga aggagctaac cgcttttttg
     4081 cacaacatgg gggatcatgt aactcgcctt gatcgttggg aaccggagct gaatgaagcc
     4141 ataccaaacg acgagcgtga caccacgatg cctgtagcaa tggcaacaac gttgcgcaaa
     4201 ctattaactg gcgaactact tactctagct tcccggcaac aattaataga ctggatggag
     4261 gcggataaag ttgcaggacc acttctgcgc tcggcccttc cggctggctg gtttattgct
     4321 gataaatctg gagccggtga gcgtgggtct cgcggtatca ttgcagcact ggggccagat
     4381 ggtaagccct cccgtatcgt agttatctac acgacgggga gtcaggcaac tatggatgaa
     4441 cgaaatagac agatcgctga gataggtgcc tcactgatta agcattggta actgtcagac
     4501 caagtttact catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc
     4561 taggtgaaga tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc
     4621 cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg
     4681 cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg
     4741 gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca
     4801 aatactgtcc ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg
     4861 cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg
     4921 tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga
     4981 acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac
     5041 ctacagcgtg agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat
     5101 ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc
     5161 tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga
     5221 tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc
     5281 ctggcctttt gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg
     5341 gataaccgta ttaccgcctt tgagtgagct gataccgctc gccgcagccg aacgaccgag
     5401 cgcagcgagt cagtgagcga ggaagcggaa gagcgcccaa tacgcaaacc gcctctcccc
     5461 gcgcgttggc cgattcatta atgcaggggg at
//
