LOCUS       sequence-395187-        9117 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Bacterial protein expression of Edc3.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9117)
  AUTHORS   Currie SL, Xing W, Muhlrad D, Decker CJ, Parker R, Rosen MK
  TITLE     Quantitative reconstitution of yeast RNA processing bodies.
  JOURNAL   Proc Natl Acad Sci U S A. 2023 Apr 4;120(14):e2214064120. doi: 
            10.1073/pnas.2214064120. Epub 2023 Mar 27.
  PUBMED    36972455
REFERENCE   2  (bases 1 to 9117)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A."; date: "2023-04-4"; volume: "120(14):e2214064120. 
            doi"; pages: " 10.1073/pnas.2214064120. Epub 2023 Mar 27"
COMMENT     Sequence Label: Edc3-EGFP
FEATURES             Location/Qualifiers
     source          1..9117
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        join(53..58,59..74,75..81)
                     /label=tac promoter
                     /note="strong E. coli promoter; hybrid between the trp and 
                     lac UV5 promoters"
     protein_bind    89..105
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     94..116
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     CDS             175..1275
                     /codon_start=1
                     /gene="malE (mutated)"
                     /product="maltose binding protein from E. coli"
                     /label=MBP
                     /note="This version of the gene does not encode a signal 
                     sequence, so MBP will remain in the cytosol."
                     /translation="MKTEEGKLVIWINGDKGYNGLAEVGKKFEKDTGIKVTVEHPDKLE
                     EKFPQVAATGDGPDIIFWAHDRFGGYAQSGLLAEITPDKAFQDKLYPFTWDAVRYNGKL
                     IAYPIAVEALSLIYNKDLLPNPPKTWEEIPALDKELKAKGKSALMFNLQEPYFTWPLIA
                     ADGGYAFKYENGKYDIKDVGVDNAGAKAGLTFLVDLIKNKHMNADTDYSIAEAAFNKGE
                     TAMTINGPWAWSNIDTSKVNYGVTVLPTFKGQPSKPFVGVLSAGINAASPNKELAKEFL
                     ENYLLTDEGLEAVNKDKPLGAVALKSYEEELAKDPRIAATMENAQKGEIMPNIPQMSAF
                     WYAVRTAVINAASGRQTVDEALKDAQT"
     regulatory      1132..1141
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     1249..1272
                     /label=MBP-F
                     /note="Maltose binding protein, forward primer"
     CDS             1324..1335
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     CDS             1348..1368
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             join(3052..3054,3055..3057,3058..3768)
                     /codon_start=1
                     /product="brightly fluorescent monomeric variant of GFP"
                     /label=mEmerald
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHKVYITADKQKNGIK
                     VNFKTRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(3097..3118)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(3358..3377)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     3705..3726
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     CDS             3781..3801
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             3802..3819
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(3832..3849)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(3832..3848)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3841..3863)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     complement(4027..4044)
                     /label=pBAD Reverse
                     /note="For vectors with E. coli araBAD promoter, reverse 
                     primer"
     terminator      4197..4283
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      4375..4402
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     promoter        4421..4512
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(4513..4581,4582..5373)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(4731..4750)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      complement(5415..5928)
                     /direction=LEFT
                     /label=M13 ori
                     /note="M13 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5610..5631)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     5822..5841
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     rep_origin      6039..6627
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6528..6547
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     6781..6798
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(6904..6923)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     7064..7086
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     CDS             complement(7080..7271)
                     /codon_start=1
                     /gene="rop"
                     /product="Rop protein, which maintains plasmids at low copy
                     number"
                     /label=rop
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
     promoter        7767..7844
                     /gene="lacI (mutant)"
                     /label=lacIq promoter
                     /note="In the lacIq allele, a single base change in the 
                     promoter boosts expression of the lacI gene about 10-fold."
     CDS             7845..8927
                     /codon_start=1
                     /gene="lacI"
                     /product="lac repressor"
                     /label=lacI
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
                     /translation="MKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAEL
                     NYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERSGV
                     EACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNVPALFLDVSDQTPINSIIFSH
                     EDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREGDWSA
                     MSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDTEDSSC
                     YIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLAPNTQTASPR
                     ALADSLMQLARQVSRLESGQ"
     primer_bind     complement(7864..7883)
                     /label=LacI-R
                     /note="LacI, reverse primer"
ORIGIN
        1 gttttttgcg ccgacatcat aacggttctg gcaaatattc tgaaatgagc tgttgacaat
       61 taatcatcgg ctcgtataat gtgtggaatt gtgagcggat aacaatttca cacaggaaac
      121 agccagtccg tttaggtgtt ttcacgagca cttcaccaac aaggaccata gattatgaaa
      181 actgaagaag gtaaactggt aatctggatt aacggcgata aaggctataa cggtctcgct
      241 gaagtcggta agaaattcga gaaagatacc ggaattaaag tcaccgttga gcatccggat
      301 aaactggaag agaaattccc acaggttgcg gcaactggcg atggccctga cattatcttc
      361 tgggcacacg accgctttgg tggctacgct caatctggcc tgttggctga aatcaccccg
      421 gacaaagcgt tccaggacaa gctgtatccg tttacctggg atgccgtacg ttacaacggc
      481 aagctgattg cttacccgat cgctgttgaa gcgttatcgc tgatttataa caaagatctg
      541 ctgccgaacc cgccaaaaac ctgggaagag atcccggcgc tggataaaga actgaaagcg
      601 aaaggtaaga gcgcgctgat gttcaacctg caagaaccgt acttcacctg gccgctgatt
      661 gctgctgacg ggggttatgc gttcaagtat gaaaacggca agtacgacat taaagacgtg
      721 ggcgtggata acgctggcgc gaaagcgggt ctgaccttcc tggttgacct gattaaaaac
      781 aaacacatga atgcagacac cgattactcc atcgcagaag ctgcctttaa taaaggcgaa
      841 acagcgatga ccatcaacgg cccgtgggca tggtccaaca tcgacaccag caaagtgaat
      901 tatggtgtaa cggtactgcc gaccttcaag ggtcaaccat ccaaaccgtt cgttggcgtg
      961 ctgagcgcag gtattaacgc cgccagtccg aacaaagagc tggcaaaaga gttcctcgaa
     1021 aactatctgc tgactgatga aggtctggaa gcggttaata aagacaaacc gctgggtgcc
     1081 gtagcgctga agtcttacga ggaagagttg gcgaaagatc cacgtattgc cgccaccatg
     1141 gaaaacgccc agaaaggtga aatcatgccg aacatcccgc agatgtccgc tttctggtat
     1201 gccgtgcgta ctgcggtgat caacgccgcc agcggtcgtc agactgtcga tgaagccctg
     1261 aaagacgcgc agactaattc gagctcgaac aacaacaaca ataacaataa caacaacctc
     1321 gggatcgagg gaaggatttc agaatttgaa aacctgtatt ttcagggcca tatgtcacaa
     1381 tttgttggtt tcggagtaca agtggagcta aaagatggga agctcattca ggggaaaatt
     1441 gccaaagcaa cctcaaaagg attgacttta aatgacgttc aattcggcga tggtggtaaa
     1501 tctcaagctt tcaaagtgag ggcgtcaagg ctaaaggact taaaggttct aactgttgcc
     1561 tctcaatccg ggaaaaggaa gcagcaaaga caacagcagc aacaaaacga ttataatcaa
     1621 aatcgcggtg aacatattga ttggcaagat gatgatgtta gtaagataaa acaacaggaa
     1681 gatttcgatt tccaaagaaa tttgggcatg tttaacaaaa aagacgtctt cgcacaatta
     1741 aagcaaaatg acgatatatt accggagaat agattacagg gacataacag aaagcaaacc
     1801 caattgcagc aaaataatta tcaaaatgat gaattggtta ttccagatgc aaagaaagat
     1861 tcatggaaca agatttcttc aagaaatgag caaagcacac accaatctca gccgcaacaa
     1921 gatgctcaag atgatctggt tttggaagat gatgaacatg aatacgatgt cgatgatatc
     1981 gatgatccca aatacctacc aataactcag tctttgaata ttacacattt aattcactct
     2041 gcaactaact ctccatccat aaatgataaa acgaaaggta cagttataaa tgataaggat
     2101 caggtactag ctaaattagg ccagatgatc atcagccagt caagatccaa ctcaacatcc
     2161 ttgccagctg caaataaaca aacaaccatc agatcaaaga acactaagca gaacattcct
     2221 atggctacac cagtacaact actagaaatg gagagcatca cgtccgaatt tttcagtatt
     2281 aactcggcag ggctactaga aaattttgct gtaaacgcat cgttcttctt aaagcagaaa
     2341 ctaggtggcc gtgcacgttt acgtttacaa aattctaatc cggaaccttt agtagtaata
     2401 ctagcctcag attccaacag atctggtgcg aaagctctgg cgttgggtag acatctttgc
     2461 caaacggggc atatccgtgt cataacatta tttacatgtt ctcaaaatga actacaggat
     2521 tccatggtca aaaagcaaac agatatttac aagaagtgtg gcggaaaaat tgttaatagt
     2581 gtatcgtcgc tggaatctgc tatggaaaca ttaaatagcc ctgtagaaat agtcatcgat
     2641 gccatgcagg gatatgactg tacattgagc gatctggcgg ggacgtcgga agttattgaa
     2701 agcagaatta aaagcatgat atcatggtgt aacaaacagc gaggatctac taaagtgtgg
     2761 tctttggata ttccaaatgg gtttgatgcg ggctcgggca tgccagatat tttcttttca
     2821 gacaggattg aagcaacagg aattatttgt tctggctggc ctttgattgc catcaacaac
     2881 ttaattgcaa atttgccaag tctagaagat gctgttttga ttgatatagg tataccacag
     2941 ggcgcctatt cacagagaac ttctttgcgt aagttccaaa actgtgatct tttcgtcact
     3001 gacgggtccc tgctattaga tttgggatcc gggtctggtt caggaatggc catggtgagc
     3061 aagggcgagg agctgttcac cggggtggtg cccatcctgg tcgagctgga cggcgacgta
     3121 aacggccaca agttcagcgt gtccggcgag ggcgagggcg atgccaccta cggcaagctg
     3181 accctgaagt tcatctgcac caccggcaag ctgcccgtgc cctggcccac cctcgtgacc
     3241 accttgacct acggcgtgca gtgcttcgcc cgctaccccg accacatgaa gcagcacgac
     3301 ttcttcaagt ccgccatgcc cgaaggctac gtccaggagc gcaccatctt cttcaaggac
     3361 gacggcaact acaagacccg cgccgaggtg aagttcgagg gcgacaccct ggtgaaccgc
     3421 atcgagctga agggcatcga cttcaaggag gacggcaaca tcctggggca caagctggag
     3481 tacaactaca acagccacaa ggtctatatc accgccgaca agcagaagaa cggcatcaag
     3541 gtgaacttca agacccgcca caacatcgag gacggcagcg tgcagctcgc cgaccactac
     3601 cagcagaaca cccccatcgg cgacggcccc gtgctgctgc ccgacaacca ctacctgagc
     3661 acccagtcca agctgagcaa agaccccaac gagaagcgcg atcacatggt cctgctggag
     3721 ttcgtgaccg ccgccgggat cactctcggc atggacgagc tgtacaaggg aagcggccgc
     3781 gaaaacctgt attttcaggg ccatcatcat catcatcact gaaagcttgg cactggccgt
     3841 cgttttacaa cgtcgtgact gggaaaaccc tggcgttacc caacttaatc gccttgcagc
     3901 acatccccct ttcgccagct ggcgtaatag cgaagaggcc cgcaccgatc gcccttccca
     3961 acagttgcgc agcctgaatg gcgaatggca gcttggctgt tttggcggat gagataagat
     4021 tttcagcctg atacagatta aatcagaacg cagaagcggt ctgataaaac agaatttgcc
     4081 tggcggcagt agcgcggtgg tcccacctga ccccatgccg aactcagaag tgaaacgccg
     4141 tagcgccgat ggtagtgtgg ggtctcccca tgcgagagta gggaactgcc aggcatcaaa
     4201 taaaacgaaa ggctcagtcg aaagactggg cctttcgttt tatctgttgt ttgtcggtga
     4261 acgctctcct gagtaggaca aatccgccgg gagcggattt gaacgttgcg aagcaacggc
     4321 ccggagggtg gcgggcagga cgcccgccat aaactgccag gcatcaaatt aagcagaagg
     4381 ccatcctgac ggatggcctt tttgcgtttc tacaaactct tttgtttatt tttctaaata
     4441 cattcaaata tgtatccgct catgagacaa taaccctgat aaatgcttca ataatattga
     4501 aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc ttattccctt ttttgcggca
     4561 ttttgccttc ctgtttttgc tcacccagaa acgctggtga aagtaaaaga tgctgaagat
     4621 cagttgggtg cacgagtggg ttacatcgaa ctggatctca acagcggtaa gatccttgag
     4681 agttttcgcc ccgaagaacg tttcccaatg atgagcactt ttaaagttct gctatgtggc
     4741 gcggtattat cccgtgttga cgccgggcaa gagcaactcg gtcgccgcat acactattct
     4801 cagaatgact tggttgagta ctcaccagtc acagaaaagc atcttacgga tggcatgaca
     4861 gtaagagaat tatgcagtgc tgccataacc atgagtgata acactgcggc caacttactt
     4921 ctgacaacga tcggaggacc gaaggagcta accgcttttt tgcacaacat gggggatcat
     4981 gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt
     5041 gacaccacga tgcctgtagc aatggcaaca acgttgcgca aactattaac tggcgaacta
     5101 cttactctag cttcccggca acaattaata gactggatgg aggcggataa agttgcagga
     5161 ccacttctgc gctcggccct tccggctggc tggtttattg ctgataaatc tggagccggt
     5221 gagcgtgggt ctcgcggtat cattgcagca ctggggccag atggtaagcc ctcccgtatc
     5281 gtagttatct acacgacggg gagtcaggca actatggatg aacgaaatag acagatcgct
     5341 gagataggtg cctcactgat taagcattgg taactgtcag accaagttta ctcatatata
     5401 ctttagattg atttaccccg gttgataatc agaaaagccc caaaaacagg aagattgtat
     5461 aagcaaatat ttaaattgta aacgttaata ttttgttaaa attcgcgtta aatttttgtt
     5521 aaatcagctc attttttaac caataggccg aaatcggcaa aatcccttat aaatcaaaag
     5581 aatagcccga gatagggttg agtgttgttc cagtttggaa caagagtcca ctattaaaga
     5641 acgtggactc caacgtcaaa gggcgaaaaa ccgtctatca gggcgatggc ccactacgtg
     5701 aaccatcacc caaatcaagt tttttggggt cgaggtgccg taaagcacta aatcggaacc
     5761 ctaaagggag cccccgattt agagcttgac ggggaaagcc ggcgaacgtg gcgagaaagg
     5821 aagggaagaa agcgaaagga gcgggcgcta gggcgctggc aagtgtagcg gtcacgctgc
     5881 gcgtaaccac cacacccgcc gcgcttaatg cgccgctaca gggcgcgtaa aaggatctag
     5941 gtgaagatcc tttttgataa tctcatgacc aaaatccctt aacgtgagtt ttcgttccac
     6001 tgagcgtcag accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc
     6061 gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat
     6121 caagagctac caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat
     6181 actgtccttc tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct
     6241 acatacctcg ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt
     6301 cttaccgggt tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg
     6361 gggggttcgt gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta
     6421 cagcgtgagc tatgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg
     6481 gtaagcggca gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg
     6541 tatctttata gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc
     6601 tcgtcagggg ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg
     6661 gccttttgct ggccttttgc tcacatgttc tttcctgcgt tatcccctga ttctgtggat
     6721 aaccgtatta ccgcctttga gtgagctgat accgctcgcc gcagccgaac gaccgagcgc
     6781 agcgagtcag tgagcgagga agcggaagag cgcctgatgc ggtattttct ccttacgcat
     6841 ctgtgcggta tttcacaccg catgtggtgc actctcagta caccgcatgt ggtgcactct
     6901 cagtacaatc tgctctgatg ccgcatagtt aagccagtat acactccgct atcgctacgt
     6961 gactgggtca tggctgcgcc ccgacacccg ccaacacccg ctgacgcgcc ctgacgggct
     7021 tgtctgctcc cggcatccgc ttacagacaa gctgtgaccg tctccgggag ctgcatgtgt
     7081 cagaggtttt caccgtcatc accgaaacgc gcgaggcagc tgcggtaaag ctcatcagcg
     7141 tggtcgtgca gcgattcaca gatgtctgcc tgttcatccg cgtccagctc gttgagtttc
     7201 tccagaagcg ttaatgtctg gcttctgata aagcgggcca tgttaagggc ggttttttcc
     7261 tgtttggtca ctgatgcctc cgtgtaaggg ggatttctgt tcatgggggt aatgataccg
     7321 atgaaacgag agaggatgct cacgatacgg gttactgatg atgaacatgc ccggttactg
     7381 gaacgttgtg agggtaaaca actggcggta tggatgcggc gggaccagag aaaaatcact
     7441 cagggtcaat gccagcgctt cgttaataca gatgtaggtg ttccacaggg tagccagcag
     7501 catcctgcga tgcagatccg gaacataatg gtgcagggcg ctgacttccg cgtttccaga
     7561 ctttacgaaa cacggaaacc gaagaccatt catgttgttg ctcaggtcgc agacgttttg
     7621 cagcagcagt cgcttcacgt tcgctcgcgt atcggtgatt cattctgcta accagtaagg
     7681 caaccccgcc agcctagccg ggtcctcaac gacaggagca cgatcatgcg cacccgtggc
     7741 caggacccaa cgctgcccga aattccgaca ccatcgaatg gtgcaaaacc tttcgcggta
     7801 tggcatgata gcgcccggaa gagagtcaat tcagggtggt gaatgtgaaa ccagtaacgt
     7861 tatacgatgt cgcagagtat gccggtgtct cttatcagac cgtttcccgc gtggtgaacc
     7921 aggccagcca cgtttctgcg aaaacgcggg aaaaagtgga agcggcgatg gcggagctga
     7981 attacattcc caaccgcgtg gcacaacaac tggcgggcaa acagtcgttg ctgattggcg
     8041 ttgccacctc cagtctggcc ctgcacgcgc cgtcgcaaat tgtcgcggcg attaaatctc
     8101 gcgccgatca actgggtgcc agcgtggtgg tgtcgatggt agaacgaagc ggcgtcgaag
     8161 cctgtaaagc ggcggtgcac aatcttctcg cgcaacgcgt cagtgggctg atcattaact
     8221 atccgctgga tgaccaggat gccattgctg tggaagctgc ctgcactaat gttccggcgt
     8281 tatttcttga tgtctctgac cagacaccca tcaacagtat tattttctcc catgaagacg
     8341 gtacgcgact gggcgtggag catctggtcg cattgggtca ccagcaaatc gcgctgttag
     8401 cgggcccatt aagttctgtc tcggcgcgtc tgcgtctggc tggctggcat aaatatctca
     8461 ctcgcaatca aattcagccg atagcggaac gggaaggcga ctggagtgcc atgtccggtt
     8521 ttcaacaaac catgcaaatg ctgaatgagg gcatcgttcc cactgcgatg ctggttgcca
     8581 acgatcagat ggcgctgggc gcaatgcgcg ccattaccga gtccgggctg cgcgttggtg
     8641 cggatatctc ggtagtggga tacgacgata ccgaagacag ctcatgttat atcccgccgt
     8701 taaccaccat caaacaggat tttcgcctgc tggggcaaac cagcgtggac cgcttgctgc
     8761 aactctctca gggccaggcg gtgaagggca atcagctgtt gcccgtctca ctggtgaaaa
     8821 gaaaaaccac cctggcgccc aatacgcaaa ccgcctctcc ccgcgcgttg gccgattcat
     8881 taatgcagct ggcacgacag gtttcccgac tggaaagcgg gcagtgagcg caacgcaatt
     8941 aatgtaagtt agctcactca ttaggcacaa ttctcatgtt tgacagctta tcatcgactg
     9001 cacggtgcac caatgcttct ggcgtcaggc agccatcgga agctgtggta tggctgtgca
     9061 ggtcgtaaat cactgcataa ttcgtgtcgc tcaaggcgca ctcccgttct ggataat
//
